(Circular) (Six-base) MAPSORT of: pFN21AB3987.seq Check: 2027 from: 1 to: 5848 pFN21AB3987 5848 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2985 3658 278 Size: 53 83 186 936 1449 673 2468 Fragments arranged by size: 2468 1449 936 673 186 83 53 AccI GT'mk_AC Cuts at: 1937 3191 1937 Size: 1254 4594 Acc65I G'GTAC_C Cuts at: 3169 3169 Size: 5848 AclI AA'CG_TT Cuts at: 1563 3975 4348 1563 Size: 2412 373 3063 Fragments arranged by size: 3063 2412 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2938 3149 3911 5295 5563 1632 Size: 1306 211 762 1384 268 1917 Fragments arranged by size: 1917 1384 1306 762 268 211 AfeI AGC'GCT Cuts at: 2990 2990 Size: 5848 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5651 AflIII A'CryG_T Cuts at: 1203 4747 5724 1203 Size: 3544 977 1327 Fragments arranged by size: 3544 1327 977 AgeI A'CCGG_T Cuts at: 4679 4679 Size: 5848 AhdI GACnn_n'nnGTC Cuts at: 4577 4577 Size: 5848 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5848 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5848 AlwNI CAG_nnn'CTG Cuts at: 2034 3591 5163 2034 Size: 1557 1572 2719 Fragments arranged by size: 2719 1572 1557 ApaI G_GGCC'C Cuts at: 2065 2065 Size: 5848 ApaLI G'TGCA_C Cuts at: 3905 5061 3905 Size: 1156 4692 ApoI r'AATT_y Cuts at: 1493 3157 3383 3535 5644 1493 Size: 1664 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 226 152 AseI AT'TA_AT Cuts at: 160 1032 4402 160 Size: 872 3370 1606 Fragments arranged by size: 3370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 AvaI C'yCGr_G Cuts at: 1870 1943 3173 1870 Size: 73 1230 4545 Fragments arranged by size: 4545 1230 73 BamHI G'GATC_C Cuts at: 3178 3545 3178 Size: 367 5481 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 3169 4624 618 Size: 325 217 627 382 1000 1455 1842 Fragments arranged by size: 1842 1455 1000 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 2065 2318 3167 1391 Size: 674 253 849 4072 Fragments arranged by size: 4072 849 674 253 BbeI G_GCGC'C Cuts at: 2173 2173 Size: 5848 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2927 928 Size: 971 1028 3849 Fragments arranged by size: 3849 1028 971 BbvCI CC'TCA_GC Cuts at: 2027 2027 Size: 5848 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4039 4073 1903 Size: 34 2102 34 3678 Fragments arranged by size: 3678 2102 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3740 4950 3740 Size: 1210 4638 BclI T'GATC_A Cuts at: 1548 1548 Size: 5848 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 4459 136 Size: 107 122 71 631 605 388 2399 1525 Fragments arranged by size: 2399 1525 631 605 388 122 107 71 BglII A'GATC_T Cuts at: 5843 5843 Size: 5848 BlpI GC'TnA_GC Cuts at: 3265 3265 Size: 5848 Bme1580I G_kGCm'C Cuts at: 1790 2065 3909 5065 1790 Size: 275 1844 1156 2573 Fragments arranged by size: 2573 1844 1156 275 BmgBI CAC'GTC Cuts at: 1318 2742 1318 Size: 1424 4424 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3579 4537 442 Size: 416 53 83 399 24 2162 958 1753 Fragments arranged by size: 2162 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2497 2749 2996 4508 1239 Size: 1058 200 252 247 1512 2579 Fragments arranged by size: 2579 1512 1058 252 247 200 Bpu10I CC'TnA_GC Cuts at: 2027 2465 2027 Size: 438 5410 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2294 2302 3973 4838 5136 2210 Size: 84 8 1671 865 298 2922 Fragments arranged by size: 2922 1671 865 298 84 8 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2764 2831 4511 882 Size: 617 1265 67 1680 2219 Fragments arranged by size: 2219 1680 1265 617 67 BsaAI yAC'GTr Cuts at: 493 1204 3639 493 Size: 711 2435 2702 Fragments arranged by size: 2702 2435 711 BsaBI GATnn'nnATC Cuts at: 2237 5842 2237 Size: 3605 2243 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2170 2982 3655 4037 Size: 53 83 186 936 637 812 673 382 Cuts at: 4037 4672 275 Size: 635 1451 Fragments arranged by size: 1451 936 812 673 637 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2565 4281 4679 4953 5100 5511 2565 Size: 1716 398 274 147 411 2902 Fragments arranged by size: 2902 1716 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5623 Fragments arranged by size: 5623 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2752 1186 Size: 1566 4282 BseYI C'CCAG_C Cuts at: 2191 2527 2703 5051 5547 2191 Size: 336 176 2348 496 2492 Fragments arranged by size: 2492 2348 496 336 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 2251 3298 4059 5087 1540 Size: 275 186 48 202 1047 761 1028 2301 Fragments arranged by size: 2301 1047 1028 761 275 202 186 48 BsiHKAI G_wGCw'C Cuts at: 3909 3994 5065 3909 Size: 85 1071 4692 Fragments arranged by size: 4692 1071 85 BsmI GAATG_Cn' Cuts at: 2352 3359 3452 2352 Size: 1007 93 4748 Fragments arranged by size: 4748 1007 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2875 3013 2875 Size: 138 5710 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2065 2318 3167 3909 3994 5065 1391 Size: 399 275 253 849 742 85 1071 2174 Fragments arranged by size: 2174 1071 849 742 399 275 253 85 BspEI T'CCGG_A Cuts at: 5511 5511 Size: 5848 BspHI T'CATG_A Cuts at: 3737 5467 3737 Size: 1730 4118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3136 3203 844 Size: 2292 67 3489 Fragments arranged by size: 3489 2292 67 BsrBI CCG'CTC Cuts at: 1394 1823 2155 2208 3735 1394 Size: 429 332 53 1527 3507 Fragments arranged by size: 3507 1527 429 332 53 BsrDI GCAATG_nn' Cuts at: 65 1248 4343 4517 65 Size: 1183 3095 174 1396 Fragments arranged by size: 3095 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 2054 2434 2808 4492 4679 1700 Size: 110 234 10 380 374 1684 187 2869 Fragments arranged by size: 2869 1684 380 374 234 187 110 10 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5848 BssHII G'CGCG_C Cuts at: 1925 2088 2090 2158 1925 Size: 163 2 68 5615 Fragments arranged by size: 5615 163 68 2 BssSI C'ACGA_G Cuts at: 2337 3908 4920 2337 Size: 1571 1012 3265 Fragments arranged by size: 3265 1571 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5848 BstBI TT'CG_AA Cuts at: 4652 4652 Size: 5848 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5848 BstXI CCAn_nnnn'nTGG Cuts at: 2812 2812 Size: 5848 BstYI r'GATC_y Cuts at: 1972 2181 2664 3178 3545 3930 3947 5399 5485 Size: 209 483 514 367 385 17 1452 86 Cuts at: 5485 5497 5843 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 514 483 385 367 346 209 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2245 513 Size: 552 1180 4116 Fragments arranged by size: 4116 1180 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5109 Fragments arranged by size: 5109 683 56 BtsI GCAGTG_nn' Cuts at: 3364 4158 4178 3364 Size: 794 20 5034 Fragments arranged by size: 5034 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5848 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5813 DraI TTT'AAA Cuts at: 3152 3152 Size: 5848 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4855 809 Size: 507 3539 1802 Fragments arranged by size: 3539 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2248 2436 Size: 54 1425 50 275 18 216 202 188 Cuts at: 2436 2572 2692 3295 4184 5813 8 Size: 136 120 603 889 1629 43 Fragments arranged by size: 1629 1425 889 603 275 216 202 188 136 120 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 2248 3295 1537 Size: 275 234 202 1047 4090 Fragments arranged by size: 4090 1047 275 234 202 EarI CTCTTCn'nnn_ Cuts at: 1353 2864 2888 3011 3047 3778 1353 Size: 1511 24 123 36 731 3423 Fragments arranged by size: 3423 1511 731 123 36 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4435 4809 4955 387 Size: 1064 138 2846 374 146 1280 Fragments arranged by size: 2846 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2497 2749 2938 2996 3149 3911 Size: 393 665 200 252 189 58 153 762 Cuts at: 3911 4508 5295 5563 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 762 665 597 393 268 252 200 189 153 58 EcoNI CCTnn'n_nnAGG Cuts at: 2789 2789 Size: 5848 EcoO109I rG'GnC_Cy Cuts at: 2061 2782 2061 Size: 721 5127 EcoRI G'AATT_C Cuts at: 3157 3535 3157 Size: 378 5470 EcoRV GAT'ATC Cuts at: 2605 2605 Size: 5848 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2686 2878 2959 3058 2686 Size: 192 81 99 5476 Fragments arranged by size: 5476 192 99 81 FspI TGC'GCA Cuts at: 2237 4354 2237 Size: 2117 3731 FspAI rTGC'GCAy Cuts at: 2237 2237 Size: 5848 HaeII r_GCGC'y Cuts at: 1678 2173 2273 2710 2992 4995 1678 Size: 495 100 437 282 2003 2531 Fragments arranged by size: 2531 2003 495 437 282 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4489 4521 4563 4595 1373 Size: 32 135 32 2917 32 42 32 2626 Fragments arranged by size: 2917 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2848 3192 3438 669 Size: 988 281 910 344 246 3079 Fragments arranged by size: 3079 988 910 344 281 246 HindIII A'AGCT_T Cuts at: 748 2345 748 Size: 1597 4251 HpaI GTT'AAC Cuts at: 3438 3438 Size: 5848 KasI G'GCGC_C Cuts at: 2169 2169 Size: 5848 KpnI G_GTAC'C Cuts at: 3173 3173 Size: 5848 MfeI C'AATT_G Cuts at: 3447 3447 Size: 5848 MluI A'CGCG_T Cuts at: 5724 5724 Size: 5848 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4962 5146 1305 Size: 3657 184 2007 Fragments arranged by size: 3657 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4026 Fragments arranged by size: 4026 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3806 4165 4324 518 Size: 648 2640 359 159 2042 Fragments arranged by size: 2640 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2034 2085 2449 2527 2578 2698 3264 3941 Size: 428 51 364 78 51 120 566 677 Cuts at: 3941 5089 5334 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 566 428 364 245 120 78 51 51 NaeI GCC'GGC Cuts at: 2046 2056 2436 2046 Size: 10 380 5458 Fragments arranged by size: 5458 380 10 NarI GG'CG_CC Cuts at: 2170 2170 Size: 5848 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5296 NdeI CA'TA_TG Cuts at: 387 387 Size: 5848 NgoMIV G'CCGG_C Cuts at: 2044 2054 2434 2044 Size: 10 380 5458 Fragments arranged by size: 5458 380 10 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5848 NotI GC'GGCC_GC Cuts at: 3295 3295 Size: 5848 NspI r_CATG'y Cuts at: 1132 1229 3206 4751 1132 Size: 97 1977 1545 2229 Fragments arranged by size: 2229 1977 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5848 PciI A'CATG_T Cuts at: 4747 4747 Size: 5848 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5848 PfoI T'CCnGG_A Cuts at: 2550 2946 2550 Size: 396 5452 PmeI GTTT'AAAC Cuts at: 3152 3152 Size: 5848 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3917 3949 5430 5462 3917 Size: 32 1481 32 4303 Fragments arranged by size: 4303 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2782 2782 Size: 5848 PshAI GACnn'nnGTC Cuts at: 1613 2843 1613 Size: 1230 4618 PsiI TTA'TAA Cuts at: 3418 3418 Size: 5848 PspOMI G'GGCC_C Cuts at: 2061 2061 Size: 5848 PstI C_TGCA'G Cuts at: 830 3120 3147 3200 830 Size: 2290 27 53 3478 Fragments arranged by size: 3478 2290 53 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5848 PvuII CAG'CTG Cuts at: 2449 2527 2578 2449 Size: 78 51 5719 Fragments arranged by size: 5719 78 51 RsrII CG'GwC_CG Cuts at: 2164 2164 Size: 5848 SalI G'TCGA_C Cuts at: 1936 3190 1936 Size: 1254 4594 SapI GCTCTTCn'nnn_ Cuts at: 2864 2864 Size: 5848 SbfI CC_TGCA'GG Cuts at: 3120 3200 3120 Size: 80 5768 ScaI AGT'ACT Cuts at: 2546 4096 5808 2546 Size: 1550 1712 2586 Fragments arranged by size: 2586 1712 1550 SexAI A'CCwGG_T Cuts at: 2778 2994 2778 Size: 216 5632 SfcI C'TryA_G Cuts at: 826 1045 3116 3143 3196 4331 5012 5203 826 Size: 219 2071 27 53 1135 681 191 1471 Fragments arranged by size: 2071 1471 1135 681 219 191 53 27 SfiI GGCCn_nnn'nGGCC Cuts at: 2060 2060 Size: 5848 SfoI GGC'GCC Cuts at: 2171 2171 Size: 5848 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5848 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5848 SmaI CCC'GGG Cuts at: 1872 3175 1872 Size: 1303 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2309 2317 3952 4853 5115 Size: 197 926 282 84 8 1635 901 262 Cuts at: 5115 820 Size: 1553 Fragments arranged by size: 1635 1553 926 901 282 262 197 84 8 SnaBI TAC'GTA Cuts at: 493 3639 493 Size: 3146 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5848 SphI G_CATG'C Cuts at: 1132 3206 1132 Size: 2074 3774 SspI AAT'ATT Cuts at: 5 52 3772 5 Size: 47 3720 2081 Fragments arranged by size: 3720 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5848 StyI C'CwwG_G Cuts at: 513 1065 2357 2628 3287 513 Size: 552 1292 271 659 3074 Fragments arranged by size: 3074 1292 659 552 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3875 3892 4045 4230 4675 325 Size: 1255 2295 17 153 185 445 1498 Fragments arranged by size: 2295 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 4094 5806 Size: 274 80 33 51 1443 567 1550 1712 Cuts at: 5806 96 Size: 138 Fragments arranged by size: 1712 1550 1443 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5808 5840 5808 Size: 32 5816 Tth111I GACn'n_nGTC Cuts at: 1316 2780 1316 Size: 1464 4384 XbaI T'CTAG_A Cuts at: 3184 3184 Size: 5848 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2635 2752 2812 2011 Size: 624 117 60 5047 Fragments arranged by size: 5047 624 117 60 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5848 XmaI C'CCGG_G Cuts at: 1870 3173 1870 Size: 1303 4545 XmnI GAAnn'nnTTC Cuts at: 2546 2717 2831 2942 3977 2546 Size: 171 114 111 1035 4417 Fragments arranged by size: 4417 1035 171 114 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2983 3656 276 Size: 53 83 186 936 1449 673 2468 Fragments arranged by size: 2468 1449 936 673 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI NruI NsiI PacI PmlI PspXI PsrI SacI SacII SanDI SrfI SwaI