Restriction map for
pFN21AB3987
(Linear) (Six-base) MAPSORT of: KIBB3987.seq Check: 8521 from: 1 to: 1143
KIBB3987.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 980 1143
Size: 980 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 933 1143
Size: 933 210
AfeI AGC'GCT
Cuts at: 0 985 1143
Size: 985 158
AlwNI CAG_nnn'CTG
Cuts at: 0 29 1143
Size: 29 1114
ApaI G_GGCC'C
Cuts at: 0 60 1143
Size: 60 1083
BanI G'GyrC_C
Cuts at: 0 164 1143
Size: 164 979
BanII G_rGCy'C
Cuts at: 0 60 313 1143
Size: 60 253 830
Fragments arranged by size:
830 253 60
BbeI G_GCGC'C
Cuts at: 0 168 1143
Size: 168 975
BbsI GAAGACnn'nnnn_
Cuts at: 0 922 1143
Size: 922 221
BbvCI CC'TCA_GC
Cuts at: 0 22 1143
Size: 22 1121
BglI GCCn_nnn'nGGC
Cuts at: 0 55 1143
Size: 55 1088
Bme1580I G_kGCm'C
Cuts at: 0 60 1143
Size: 60 1083
BmgBI CAC'GTC
Cuts at: 0 737 1143
Size: 737 406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 492 744 991 1143
Size: 292 200 252 247 152
Fragments arranged by size:
292 252 247 200 152
Bpu10I CC'TnA_GC
Cuts at: 0 22 460 1143
Size: 22 438 683
Fragments arranged by size:
683 438 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 205 289 297 1143
Size: 205 84 8 846
Fragments arranged by size:
846 205 84 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 759 826 1143
Size: 759 67 317
Fragments arranged by size:
759 317 67
BsaBI GATnn'nnATC
Cuts at: 0 232 1143
Size: 232 911
BsaHI Gr'CG_yC
Cuts at: 0 165 977 1143
Size: 165 812 166
Fragments arranged by size:
812 166 165
BsaWI w'CCGG_w
Cuts at: 0 560 1143
Size: 560 583
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 747 1143
Size: 747 396
BseYI C'CCAG_C
Cuts at: 0 186 522 698 1143
Size: 186 336 176 445
Fragments arranged by size:
445 336 186 176
BsiEI CG_ry'CG
Cuts at: 0 44 246 1143
Size: 44 202 897
Fragments arranged by size:
897 202 44
BsmI GAATG_Cn'
Cuts at: 0 347 1143
Size: 347 796
BsmBI CGTCTCn'nnnn_
Cuts at: 0 870 1008 1143
Size: 870 138 135
Fragments arranged by size:
870 138 135
Bsp1286I G_dGCh'C
Cuts at: 0 60 313 1143
Size: 60 253 830
Fragments arranged by size:
830 253 60
BsrBI CCG'CTC
Cuts at: 0 150 203 1143
Size: 150 53 940
Fragments arranged by size:
940 150 53
BsrFI r'CCGG_y
Cuts at: 0 39 49 429 803 1143
Size: 39 10 380 374 340
Fragments arranged by size:
380 374 340 39 10
BssHII G'CGCG_C
Cuts at: 0 83 85 153 1143
Size: 83 2 68 990
Fragments arranged by size:
990 83 68 2
BssSI C'ACGA_G
Cuts at: 0 332 1143
Size: 332 811
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 1143
Size: 807 336
BstYI r'GATC_y
Cuts at: 0 176 659 1143
Size: 176 483 484
Fragments arranged by size:
484 483 176
BtgI C'CryG_G
Cuts at: 0 240 1143
Size: 240 903
EaeI y'GGCC_r
Cuts at: 0 41 243 431 567 687 1143
Size: 41 202 188 136 120 456
Fragments arranged by size:
456 202 188 136 120 41
EagI C'GGCC_G
Cuts at: 0 41 243 1143
Size: 41 202 900
Fragments arranged by size:
900 202 41
EarI CTCTTCn'nnn_
Cuts at: 0 859 883 1006 1042 1143
Size: 859 24 123 36 101
Fragments arranged by size:
859 123 101 36 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 492 744 933 991 1143
Size: 292 200 252 189 58 152
Fragments arranged by size:
292 252 200 189 152 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 784 1143
Size: 784 359
EcoO109I rG'GnC_Cy
Cuts at: 0 56 777 1143
Size: 56 721 366
Fragments arranged by size:
721 366 56
EcoRV GAT'ATC
Cuts at: 0 600 1143
Size: 600 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 681 873 954 1053 1143
Size: 681 192 81 99 90
Fragments arranged by size:
681 192 99 90 81
FspI TGC'GCA
Cuts at: 0 232 1143
Size: 232 911
FspAI rTGC'GCAy
Cuts at: 0 232 1143
Size: 232 911
HaeII r_GCGC'y
Cuts at: 0 168 268 705 987 1143
Size: 168 100 437 282 156
Fragments arranged by size:
437 282 168 156 100
HincII GTy'rAC
Cuts at: 0 843 1143
Size: 843 300
HindIII A'AGCT_T
Cuts at: 0 340 1143
Size: 340 803
KasI G'GCGC_C
Cuts at: 0 164 1143
Size: 164 979
MspA1I CmG'CkG
Cuts at: 0 29 80 444 522 573 693 1143
Size: 29 51 364 78 51 120 450
Fragments arranged by size:
450 364 120 78 51 51 29
NaeI GCC'GGC
Cuts at: 0 41 51 431 1143
Size: 41 10 380 712
Fragments arranged by size:
712 380 41 10
NarI GG'CG_CC
Cuts at: 0 165 1143
Size: 165 978
NgoMIV G'CCGG_C
Cuts at: 0 39 49 429 1143
Size: 39 10 380 714
Fragments arranged by size:
714 380 39 10
PfoI T'CCnGG_A
Cuts at: 0 545 941 1143
Size: 545 396 202
Fragments arranged by size:
545 396 202
PpuMI rG'GwC_Cy
Cuts at: 0 777 1143
Size: 777 366
PshAI GACnn'nnGTC
Cuts at: 0 838 1143
Size: 838 305
PspOMI G'GGCC_C
Cuts at: 0 56 1143
Size: 56 1087
PstI C_TGCA'G
Cuts at: 0 1115 1142 1143
Size: 1115 27 1
Fragments arranged by size:
1115 27 1
PvuII CAG'CTG
Cuts at: 0 444 522 573 1143
Size: 444 78 51 570
Fragments arranged by size:
570 444 78 51
RsrII CG'GwC_CG
Cuts at: 0 159 1143
Size: 159 984
SapI GCTCTTCn'nnn_
Cuts at: 0 859 1143
Size: 859 284
SbfI CC_TGCA'GG
Cuts at: 0 1115 1143
Size: 1115 28
ScaI AGT'ACT
Cuts at: 0 541 1143
Size: 541 602
SexAI A'CCwGG_T
Cuts at: 0 773 989 1143
Size: 773 216 154
Fragments arranged by size:
773 216 154
SfcI C'TryA_G
Cuts at: 0 1111 1138 1143
Size: 1111 27 5
Fragments arranged by size:
1111 27 5
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 55 1143
Size: 55 1088
SfoI GGC'GCC
Cuts at: 0 166 1143
Size: 166 977
SmlI C'TyrA_G
Cuts at: 0 220 304 312 1143
Size: 220 84 8 831
Fragments arranged by size:
831 220 84 8
StyI C'CwwG_G
Cuts at: 0 352 623 1143
Size: 352 271 520
Fragments arranged by size:
520 352 271
TatI w'GTAC_w
Cuts at: 0 539 1143
Size: 539 604
Tth111I GACn'n_nGTC
Cuts at: 0 775 1143
Size: 775 368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 630 747 807 1143
Size: 630 117 60 336
Fragments arranged by size:
630 336 117 60
XmnI GAAnn'nnTTC
Cuts at: 0 541 712 826 937 1143
Size: 541 171 114 111 206
Fragments arranged by size:
541 206 171 114 111
ZraI GAC'GTC
Cuts at: 0 978 1143
Size: 978 165
Enzymes that do cut:
AatII AcuI AfeI AlwNI ApaI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsmI
BsmBI Bsp1286I BsrBI BsrFI BssHII BssSI BstXI BstYI
BtgI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI FspAI HaeII HincII HindIII KasI MspA1I
NaeI NarI NgoMIV PfoI PpuMI PshAI PspOMI PstI
PvuII RsrII SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmlI StyI TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BglII BlpI BmrI BmtI BplI BsaAI BsaXI BsgI
BsiHKAI BsiWI BspEI BspHI BspMI BsrDI BsrGI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI EcoRI FseI Hin4I
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI SacI SacII SalI SanDI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI XbaI XhoI XmaI