(Circular) (Six-base) MAPSORT of: pFN21AB3955.seq Check: 1590 from: 1 to: 6067 pFN21AB3955 6067 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2629 3877 278 Size: 53 83 186 936 1093 1248 2468 Fragments arranged by size: 2468 1248 1093 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3168 3410 1937 Size: 1231 242 4594 Fragments arranged by size: 4594 1231 242 Acc65I G'GTAC_C Cuts at: 2191 3388 2191 Size: 1197 4870 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2924 3060 3257 4130 5514 5782 1632 Size: 1292 136 197 873 1384 268 1917 Fragments arranged by size: 1917 1384 1292 873 268 197 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 3360 4966 5943 1203 Size: 2157 1606 977 1327 Fragments arranged by size: 2157 1606 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 2435 1166 Size: 1269 4798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3810 5382 3810 Size: 1572 4495 ApaI G_GGCC'C Cuts at: 2828 2828 Size: 6067 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 2102 2333 2867 2983 3020 3376 3602 3754 Size: 609 231 534 116 37 356 226 152 Cuts at: 3754 5863 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 609 534 356 231 226 152 116 37 AseI AT'TA_AT Cuts at: 160 1032 4621 160 Size: 872 3589 1606 Fragments arranged by size: 3589 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 3392 1870 Size: 73 1449 4545 Fragments arranged by size: 4545 1449 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2191 3388 4843 618 Size: 325 217 627 404 1197 1455 1842 Fragments arranged by size: 1842 1455 1197 627 404 325 217 BanII G_rGCy'C Cuts at: 1391 2828 3386 1391 Size: 1437 558 4072 Fragments arranged by size: 4072 1437 558 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3245 928 Size: 971 1346 3750 Fragments arranged by size: 3750 1346 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5169 3959 Size: 1210 4857 BclI T'GATC_A Cuts at: 1548 2377 1548 Size: 829 5238 BfrBI ATG'CAT Cuts at: 2456 2456 Size: 6067 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2116 2149 6062 2116 Size: 33 3913 2121 Fragments arranged by size: 3913 2121 33 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 2828 4128 5284 1790 Size: 1038 1300 1156 2573 Fragments arranged by size: 2573 1300 1156 1038 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2699 3798 4756 Size: 416 53 83 399 24 1282 1099 958 Cuts at: 4756 442 Size: 1753 Fragments arranged by size: 1753 1282 1099 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3174 4727 1239 Size: 1935 1553 2579 Fragments arranged by size: 2579 1935 1553 Bpu10I CC'TnA_GC Cuts at: 2187 2187 Size: 6067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4730 882 Size: 617 3231 2219 Fragments arranged by size: 3231 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3361 3858 493 Size: 711 2157 497 2702 Fragments arranged by size: 2702 2157 711 497 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2626 3874 4256 4891 Size: 53 83 186 936 1093 1248 382 635 Cuts at: 4891 275 Size: 1451 Fragments arranged by size: 1451 1248 1093 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4500 4898 5172 5319 5730 4500 Size: 398 274 147 411 4837 Fragments arranged by size: 4837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2053 2083 1178 Size: 30 165 30 650 30 5162 Fragments arranged by size: 5162 650 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2751 3281 1186 Size: 1565 530 3972 Fragments arranged by size: 3972 1565 530 BseYI C'CCAG_C Cuts at: 2500 5270 5766 2500 Size: 2770 496 2801 Fragments arranged by size: 2801 2770 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5494 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4128 4213 5284 4128 Size: 85 1071 4911 Fragments arranged by size: 4911 1071 85 BsmI GAATG_Cn' Cuts at: 3063 3578 3671 3063 Size: 515 93 5459 Fragments arranged by size: 5459 515 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2828 3386 4128 4213 5284 1391 Size: 399 1038 558 742 85 1071 2174 Fragments arranged by size: 2174 1071 1038 742 558 399 85 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 2125 3956 5686 2125 Size: 1831 1730 2506 Fragments arranged by size: 2506 1831 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3954 1394 Size: 429 2131 3507 Fragments arranged by size: 3507 2131 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4562 4736 65 Size: 1183 3314 174 1396 Fragments arranged by size: 3314 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2358 96 Size: 2262 3805 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 2041 1184 Size: 857 5210 BstYI r'GATC_y Cuts at: 1972 2116 2149 3397 3764 4149 4166 5618 5704 Size: 144 33 1248 367 385 17 1452 86 Cuts at: 5704 5716 6062 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1248 385 367 346 144 86 33 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 2724 3204 513 Size: 552 939 543 177 480 3376 Fragments arranged by size: 3376 939 552 543 480 177 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2502 487 Size: 683 56 1276 4052 Fragments arranged by size: 4052 1276 683 56 BtsI GCAGTG_nn' Cuts at: 2444 2740 3230 3583 4377 4397 2444 Size: 296 490 353 794 20 4114 Fragments arranged by size: 4114 794 490 353 296 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 2332 3025 3371 2332 Size: 693 346 5028 Fragments arranged by size: 5028 693 346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2739 3514 4403 Size: 54 1425 50 275 18 909 775 889 Cuts at: 4403 6032 8 Size: 1629 43 Fragments arranged by size: 1629 1425 909 889 775 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2970 3036 3997 1353 Size: 1617 66 961 3423 Fragments arranged by size: 3423 1617 961 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2924 3060 3174 3257 4130 4727 5514 Size: 393 1292 136 114 83 873 597 787 Cuts at: 5514 5782 1239 Size: 268 1524 Fragments arranged by size: 1524 1292 873 787 597 393 268 136 114 83 EcoO109I rG'GnC_Cy Cuts at: 2403 2824 2825 2912 2403 Size: 421 1 87 5558 Fragments arranged by size: 5558 421 87 1 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2259 2259 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4708 4740 4782 4814 1373 Size: 32 135 32 3136 32 42 32 2626 Fragments arranged by size: 3136 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3411 3657 669 Size: 988 281 1473 246 3079 Fragments arranged by size: 3079 1473 988 281 246 HindIII A'AGCT_T Cuts at: 748 2749 748 Size: 2001 4066 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 2195 3392 2195 Size: 1197 4870 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 5181 5365 1305 Size: 1128 2748 184 2007 Fragments arranged by size: 2748 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2435 3294 4025 4384 4543 518 Size: 648 1269 859 731 359 159 2042 Fragments arranged by size: 2042 1269 859 731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3483 4160 5308 5553 1606 Size: 1877 677 1148 245 2120 Fragments arranged by size: 2120 1877 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2547 2724 3204 513 Size: 552 939 543 177 480 3376 Fragments arranged by size: 3376 939 552 543 480 177 NdeI CA'TA_TG Cuts at: 387 2797 387 Size: 2410 3657 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NsiI A_TGCA'T Cuts at: 2458 2458 Size: 6067 NspI r_CATG'y Cuts at: 1132 1229 3316 3425 4970 1132 Size: 97 2087 109 1545 2229 Fragments arranged by size: 2229 2087 1545 109 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2912 2912 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PspOMI G'GGCC_C Cuts at: 2824 2824 Size: 6067 PstI C_TGCA'G Cuts at: 830 2315 3419 830 Size: 1485 1104 3478 Fragments arranged by size: 3478 1485 1104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SapI GCTCTTCn'nnn_ Cuts at: 2970 2970 Size: 6067 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2311 3415 4550 5231 5422 826 Size: 219 1266 1104 1135 681 191 1471 Fragments arranged by size: 1471 1266 1135 1104 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4171 5072 5334 820 Size: 197 926 2228 901 262 1553 Fragments arranged by size: 2228 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 3991 5 Size: 47 3939 2081 Fragments arranged by size: 3939 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2615 2724 3204 3506 513 Size: 552 939 543 68 109 480 302 3074 Fragments arranged by size: 3074 939 552 543 480 302 109 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2358 4313 6025 Size: 274 80 33 51 1443 381 1955 1712 Cuts at: 6025 96 Size: 138 Fragments arranged by size: 1955 1712 1443 381 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6027 6059 6027 Size: 32 6035 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6067 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2142 4196 2142 Size: 2054 4013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2627 3875 276 Size: 53 83 186 936 1093 1248 2468 Fragments arranged by size: 2468 1248 1093 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI