(Circular) (Six-base) MAPSORT of: pFN21AB3938.seq Check: 9995 from: 1 to: 6031 pFN21AB3938 6031 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2215 2215 Size: 6031 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3841 278 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2481 3374 1937 Size: 544 893 4594 Fragments arranged by size: 4594 893 544 Acc65I G'GTAC_C Cuts at: 3352 3352 Size: 6031 AclI AA'CG_TT Cuts at: 1563 4158 4531 1563 Size: 2595 373 3063 Fragments arranged by size: 3063 2595 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2730 3059 4094 5478 5746 1632 Size: 1098 329 1035 1384 268 1917 Fragments arranged by size: 1917 1384 1098 1035 329 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5834 AflIII A'CryG_T Cuts at: 1203 4930 5907 1203 Size: 3727 977 1327 Fragments arranged by size: 3727 1327 977 AgeI A'CCGG_T Cuts at: 4862 4862 Size: 6031 AhdI GACnn_n'nnGTC Cuts at: 4760 4760 Size: 6031 AleI CACnn'nnGTG Cuts at: 1166 2321 1166 Size: 1155 4876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6031 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2628 2660 2628 Size: 32 5999 AlwNI CAG_nnn'CTG Cuts at: 3774 5346 3774 Size: 1572 4459 ApaLI G'TGCA_C Cuts at: 4088 5244 4088 Size: 1156 4875 ApoI r'AATT_y Cuts at: 1493 3136 3340 3566 3718 5827 1493 Size: 1643 204 226 152 2109 1697 Fragments arranged by size: 2109 1697 1643 226 204 152 AseI AT'TA_AT Cuts at: 160 1032 4585 160 Size: 872 3553 1606 Fragments arranged by size: 3553 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 AvaI C'yCGr_G Cuts at: 1870 1943 3356 1870 Size: 73 1413 4545 Fragments arranged by size: 4545 1413 73 BamHI G'GATC_C Cuts at: 3361 3728 3361 Size: 367 5664 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2203 2215 2533 3216 3352 Size: 325 217 627 416 12 318 683 136 Cuts at: 3352 4807 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 683 627 416 325 318 217 136 12 BanII G_rGCy'C Cuts at: 1391 3350 1391 Size: 1959 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2795 928 Size: 971 896 4164 Fragments arranged by size: 4164 971 896 BbvCI CC'TCA_GC Cuts at: 3026 3026 Size: 6031 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4222 4256 1903 Size: 34 50 34 2201 34 3678 Fragments arranged by size: 3678 2201 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3923 5133 3923 Size: 1210 4821 BclI T'GATC_A Cuts at: 1548 2941 3283 1548 Size: 1393 342 4296 Fragments arranged by size: 4296 1393 342 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2189 2432 4642 Size: 107 122 71 631 605 517 243 2210 Cuts at: 4642 136 Size: 1525 Fragments arranged by size: 2210 1525 631 605 517 243 122 107 71 BglII A'GATC_T Cuts at: 6026 6026 Size: 6031 BlpI GC'TnA_GC Cuts at: 3448 3448 Size: 6031 Bme1580I G_kGCm'C Cuts at: 1790 4092 5248 1790 Size: 2302 1156 2573 Fragments arranged by size: 2573 2302 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6031 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3762 4720 442 Size: 416 53 83 399 24 2345 958 1753 Fragments arranged by size: 2345 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6031 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4691 1239 Size: 3452 2579 Bpu10I CC'TnA_GC Cuts at: 3026 3026 Size: 6031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4156 5021 5319 4156 Size: 865 298 4868 Fragments arranged by size: 4868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2436 2692 4694 882 Size: 617 937 256 2002 2219 Fragments arranged by size: 2219 2002 937 617 256 BsaAI yAC'GTr Cuts at: 493 1204 3822 493 Size: 711 2618 2702 Fragments arranged by size: 2702 2618 711 BsaBI GATnn'nnATC Cuts at: 6025 6025 Size: 6031 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2144 3838 4220 4855 Size: 53 83 186 936 611 1694 382 635 Cuts at: 4855 275 Size: 1451 Fragments arranged by size: 1694 1451 936 635 611 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4464 4862 5136 5283 5694 4464 Size: 398 274 147 411 4801 Fragments arranged by size: 4801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5806 Fragments arranged by size: 5806 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 1186 Size: 913 5118 BseYI C'CCAG_C Cuts at: 2344 5234 5730 2344 Size: 2890 496 2645 Fragments arranged by size: 2890 2645 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 1163 Size: 573 699 4759 Fragments arranged by size: 4759 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2198 3481 4242 5270 1540 Size: 275 186 197 1283 761 1028 2301 Fragments arranged by size: 2301 1283 1028 761 275 197 186 BsiHKAI G_wGCw'C Cuts at: 2587 4092 4177 5248 2587 Size: 1505 85 1071 3370 Fragments arranged by size: 3370 1505 1071 85 BsmI GAATG_Cn' Cuts at: 3542 3635 3542 Size: 93 5938 BsmBI CGTCTCn'nnnn_ Cuts at: 2950 2950 Size: 6031 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2587 3350 4092 4177 5248 1391 Size: 399 797 763 742 85 1071 2174 Fragments arranged by size: 2174 1071 797 763 742 399 85 BspEI T'CCGG_A Cuts at: 5694 5694 Size: 6031 BspHI T'CATG_A Cuts at: 3920 5650 3920 Size: 1730 4301 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2454 3386 844 Size: 1371 239 932 3489 Fragments arranged by size: 3489 1371 932 239 BsrBI CCG'CTC Cuts at: 1394 1823 3918 1394 Size: 429 2095 3507 Fragments arranged by size: 3507 2095 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4526 4700 65 Size: 1183 3278 174 1396 Fragments arranged by size: 3278 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4675 4862 1700 Size: 110 2865 187 2869 Fragments arranged by size: 2869 2865 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6031 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6031 BssSI C'ACGA_G Cuts at: 4091 5103 4091 Size: 1012 5019 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6031 BstBI TT'CG_AA Cuts at: 4835 4835 Size: 6031 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6031 BstYI r'GATC_y Cuts at: 1972 2073 2240 3361 3728 4113 4130 5582 5668 Size: 101 167 1121 367 385 17 1452 86 Cuts at: 5668 5680 6026 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1121 385 367 346 167 101 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2556 513 Size: 552 1491 3988 Fragments arranged by size: 3988 1491 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5292 Fragments arranged by size: 5292 683 56 BtsI GCAGTG_nn' Cuts at: 2868 3547 4341 4361 2868 Size: 679 794 20 4538 Fragments arranged by size: 4538 794 679 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6031 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5996 DraI TTT'AAA Cuts at: 2937 3335 2937 Size: 398 5633 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5038 809 Size: 507 3722 1802 Fragments arranged by size: 3722 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2504 2862 2967 Size: 54 1425 50 275 18 674 358 105 Cuts at: 2967 3478 4367 5996 8 Size: 511 889 1629 43 Fragments arranged by size: 1629 1425 889 674 511 358 275 105 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3478 1537 Size: 275 1666 4090 Fragments arranged by size: 4090 1666 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3961 1353 Size: 2608 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2235 4618 4992 5138 387 Size: 1064 138 646 2383 374 146 1280 Fragments arranged by size: 2383 1280 1064 646 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2730 3059 4094 4691 5478 5746 1239 Size: 393 1098 329 1035 597 787 268 1524 Fragments arranged by size: 1524 1098 1035 787 597 393 329 268 EcoO109I rG'GnC_Cy Cuts at: 2279 2279 Size: 6031 EcoRI G'AATT_C Cuts at: 3136 3340 3718 3136 Size: 204 378 5449 Fragments arranged by size: 5449 378 204 EcoRV GAT'ATC Cuts at: 2603 2603 Size: 6031 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3220 3220 Size: 6031 FspI TGC'GCA Cuts at: 4537 4537 Size: 6031 HaeII r_GCGC'y Cuts at: 1678 5178 1678 Size: 3500 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4672 4704 4746 4778 1373 Size: 32 135 32 3100 32 42 32 2626 Fragments arranged by size: 3100 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3375 3621 669 Size: 988 281 1437 246 3079 Fragments arranged by size: 3079 1437 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6031 HpaI GTT'AAC Cuts at: 3621 3621 Size: 6031 KpnI G_GTAC'C Cuts at: 3356 3356 Size: 6031 MfeI C'AATT_G Cuts at: 3153 3630 3153 Size: 477 5554 MluI A'CGCG_T Cuts at: 5907 5907 Size: 6031 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 5145 5329 1305 Size: 897 2943 184 2007 Fragments arranged by size: 2943 2007 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2506 2864 2969 10 Size: 54 1425 343 674 358 105 3072 Fragments arranged by size: 3072 1425 674 358 343 105 54 MslI CAynn'nnrTG Cuts at: 518 1166 2321 3205 3291 3989 4348 4507 518 Size: 648 1155 884 86 698 359 159 2042 Fragments arranged by size: 2042 1155 884 698 648 359 159 86 MspA1I CmG'CkG Cuts at: 1606 3447 4124 5272 5517 1606 Size: 1841 677 1148 245 2120 Fragments arranged by size: 2120 1841 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5479 NdeI CA'TA_TG Cuts at: 387 387 Size: 6031 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6031 NotI GC'GGCC_GC Cuts at: 3478 3478 Size: 6031 NspI r_CATG'y Cuts at: 1132 1229 2401 2926 3389 4934 1132 Size: 97 1172 525 463 1545 2229 Fragments arranged by size: 2229 1545 1172 525 463 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6031 PciI A'CATG_T Cuts at: 4930 4930 Size: 6031 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6031 PmeI GTTT'AAAC Cuts at: 3335 3335 Size: 6031 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4100 4132 5613 5645 4100 Size: 32 1481 32 4486 Fragments arranged by size: 4486 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2279 2279 Size: 6031 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6031 PsiI TTA'TAA Cuts at: 3054 3601 3054 Size: 547 5484 PstI C_TGCA'G Cuts at: 830 3383 830 Size: 2553 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6031 SalI G'TCGA_C Cuts at: 1936 3373 1936 Size: 1437 4594 SbfI CC_TGCA'GG Cuts at: 3383 3383 Size: 6031 ScaI AGT'ACT Cuts at: 4279 5991 4279 Size: 1712 4319 SfcI C'TryA_G Cuts at: 826 1045 3379 4514 5195 5386 826 Size: 219 2334 1135 681 191 1471 Fragments arranged by size: 2334 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6031 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6031 SmaI CCC'GGG Cuts at: 1872 3358 1872 Size: 1486 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4135 5036 5298 820 Size: 197 926 2192 901 262 1553 Fragments arranged by size: 2192 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3822 493 Size: 3329 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6031 SphI G_CATG'C Cuts at: 1132 2401 2926 3389 1132 Size: 1269 525 463 3774 Fragments arranged by size: 3774 1269 525 463 SspI AAT'ATT Cuts at: 5 52 3955 5 Size: 47 3903 2081 Fragments arranged by size: 3903 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6031 StyI C'CwwG_G Cuts at: 513 1065 2169 2275 3243 3470 513 Size: 552 1104 106 968 227 3074 Fragments arranged by size: 3074 1104 968 552 227 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 2671 4058 4075 4228 4413 4858 Size: 1255 584 507 1387 17 153 185 445 Cuts at: 4858 325 Size: 1498 Fragments arranged by size: 1498 1387 1255 584 507 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4277 5989 96 Size: 274 80 33 51 1443 2300 1712 138 Fragments arranged by size: 2300 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2553 2585 5991 6023 2553 Size: 32 3406 32 2561 Fragments arranged by size: 3406 2561 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6031 XbaI T'CTAG_A Cuts at: 3367 3367 Size: 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 2434 2185 Size: 249 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6031 XmaI C'CCGG_G Cuts at: 1870 3356 1870 Size: 1486 4545 XmnI GAAnn'nnTTC Cuts at: 4160 4160 Size: 6031 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3839 276 Size: 53 83 186 936 2305 2468 Fragments arranged by size: 2468 2305 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI