Restriction map for
pFN21AB3867
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BlnI BsaAI BsaI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspHI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MfeI MluI MluNI
MscI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I XbaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccI 217 GTCTAC GT^[AC][GT]AC
AccII 678 CGCG CG^CG
AccIII 184 TCCGGA T^CCGGA
AgeI 425 ACCGGT A^CCGGT
AlwNI 235 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 700 GTGCTC G[AT]GC[AT]^C
AspI 1009 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 428 GGTCC G^G[AT]CC
BamHI 635 GGATCC G^GATCC
BanII 352 GAGCCC G[GA]GC[CT]^C
BcgI 722 CGAACCGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 816 AGATCT A^GATCT
BlpI 681 GCTCAGC GC^T[ACGT]AGC
BpmI 122 CTGGAG CTGGAG(16/14)
Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC
BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 184 TCCGGA T^CCGGA
BseRI 22 GAGGAG GAGGAG(10/8)
BsiHKAI 700 GTGCTC G[AT]GC[AT]^C
BsmAI 738 GTCTC GTCTC(1/5)
BspDI 781 ATCGAT AT^CGAT
BspEI 184 TCCGGA T^CCGGA
BsrFI 425 ACCGGT [GA]^CCGG[CT]
BssSI 733 CACGAG CACGAG(-5/-1)
BstUI 678 CGCG CG^CG
BstXI 168 CCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 681 GCTCAGC GC^T[ACGT]AGC
Cfr10I 425 ACCGGT [GA]^CCGG[CT]
ClaI 781 ATCGAT AT^CGAT
DraII 876 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 260 CCATGG C^C[GA][CT]GG
EaeI 659 CGGCCA [CT]^GGCC[GA]
EcoNI 66 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 876 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 260 CCATGG C^C[AT][AT]GG
HaeII 85 AGCGCC [GA]GCGC^[CT]
MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 184 TCCGGA T^CCGGA
MspA1I 684 CAGCGG C[AC]G^C[GT]G
MvnI 678 CGCG CG^CG
NcoI 260 CCATGG C^CATGG
NspI 728 GCATGC [GA]CATG^[CT]
PaeR7I 37 CTCGAG C^TCGAG
PflFI 1009 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 234 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 425 ACCGGT A^CCGGT
PstI 362 CTGCAG CTGCA^G
SapI 747 GCTCTTC GCTCTTC(1/4)
SexAI 695 ACCAGGT A^CC[AT]GGT
SmaI 431 CCCGGG CCC^GGG
SmlI 37 CTCGAG C^T[CT][GA]AG
SphI 728 GCATGC GCATG^C
StyI 260 CCATGG C^C[AT][AT]GG
Tth111I 1009 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 234 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 37 CTCGAG C^TCGAG
XmaCI 431 CCCGGG C^CCGGG
XmaI 431 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 601 GACGTC GACGT^C
652 GACGTC
AluI 368 AGCT AG^CT
443 AGCT
BmyI 352 GAGCCC G[AGT]GC[ACT]^C
700 GTGCTC
BsaWI 184 TCCGGA [AT]^CCGG[AT]
425 ACCGGT
BsmFI 237 GTCCC GGGAC(10/14)
429 GTCCC
Bsp1286I 352 GAGCCC G[AGT]GC[ACT]^C
700 GTGCTC
BsrBI 936 CCGCTC CCGCTC(-3/-3)
40 GAGCGG
BsrDI 640 CATTGC GCAATG(2/0)
1029 CATTGC
BsrI 547 ACTGG ACTGG(1/-1)
234 CCAGT
BstYI 635 GGATCC [GA]^GATC[CT]
816 AGATCT
Cac8I 611 GCCAGC GC[ACGT]^[ACGT]GC
728 GCATGC
EarI 748 CTCTTC CTCTTC(1/4)
799 CTCTTC
Ksp632I 748 CTCTTC CTCTTC(1/4)
799 CTCTTC
MflI 635 GGATCC [GA]^GATC[CT]
816 AGATCT
MwoI 507 GCCCACTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
929 GCAAAAACCGC
SfcI 219 CTACAG C^T[GA][CT]AG
362 CTGCAG
TfiI 127 GAATC G^A[AT]TC
407 GAATC
Tsp509I 104 AATT ^AATT
920 AATT
TspRI 510 CACTG CA[GC]TG[ACGT][ACGT]^
546 CACTG
XcmI 260 CCATGGACACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
458 CCAAATACTACCTGG
XhoII 635 GGATCC [GA]^GATC[CT]
816 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AciI AcyI AfaI AlwI AvaI
BbsI BbvI BcnI BpuAI BsaHI
BsaJI BsiYI BslI BsoBI BssKI
BstF5I BstNI CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TthHB8I