(Circular) (Six-base) MAPSORT of: pFN21AB3867.seq Check: 8304 from: 1 to: 5767 pFN21AB3867 5767 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2610 2661 3577 278 Size: 53 83 186 936 1074 51 916 2468 Fragments arranged by size: 2468 1074 936 916 186 83 53 51 AccI GT'mk_AC Cuts at: 1937 2223 3110 1937 Size: 286 887 4594 Fragments arranged by size: 4594 887 286 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2578 3830 5214 5482 1632 Size: 946 1252 1384 268 1917 Fragments arranged by size: 1917 1384 1252 946 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 4666 5643 1203 Size: 3463 977 1327 Fragments arranged by size: 3463 1327 977 AgeI A'CCGG_T Cuts at: 2430 4598 2430 Size: 2168 3599 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 2150 1166 Size: 984 4783 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2245 3510 5082 2245 Size: 1265 1572 2930 Fragments arranged by size: 2930 1572 1265 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 3076 3302 3454 5563 1493 Size: 1583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1583 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2120 2436 3092 1870 Size: 73 99 78 316 656 4545 Fragments arranged by size: 4545 656 316 99 78 73 BamHI G'GATC_C Cuts at: 2640 3097 3464 2640 Size: 457 367 4943 Fragments arranged by size: 4943 457 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3088 4543 618 Size: 325 217 627 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 325 217 BanII G_rGCy'C Cuts at: 1391 2361 3086 1391 Size: 970 725 4072 Fragments arranged by size: 4072 970 725 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2202 2355 2721 2904 928 Size: 971 303 153 366 183 3791 Fragments arranged by size: 3791 971 366 303 183 153 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2716 2750 3958 3992 1903 Size: 34 779 34 1208 34 3678 Fragments arranged by size: 3678 1208 779 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 1548 Size: 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2821 5762 2821 Size: 2941 2826 BlpI GC'TnA_GC Cuts at: 2687 3184 2687 Size: 497 5270 Bme1580I G_kGCm'C Cuts at: 1790 3828 4984 1790 Size: 2038 1156 2573 Fragments arranged by size: 2573 2038 1156 BmgBI CAC'GTC Cuts at: 1318 2104 2200 1318 Size: 786 96 4885 Fragments arranged by size: 4885 786 96 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3498 4456 442 Size: 416 53 83 399 24 2081 958 1753 Fragments arranged by size: 2081 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2057 2380 2057 Size: 323 5444 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2148 4427 1239 Size: 909 2279 2579 Fragments arranged by size: 2579 2279 909 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3892 4757 5055 3892 Size: 865 298 4604 Fragments arranged by size: 4604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 2165 5761 2165 Size: 3596 2171 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2313 2607 2658 3015 Size: 53 83 186 936 780 294 51 357 Cuts at: 3015 3574 3956 4591 275 Size: 559 382 635 1451 Fragments arranged by size: 1451 936 780 635 559 382 357 294 186 83 53 51 BsaWI w'CCGG_w Cuts at: 2189 2430 4200 4598 4872 5019 5430 2189 Size: 241 1770 398 274 147 411 2526 Fragments arranged by size: 2526 1770 411 398 274 241 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2184 2214 1178 Size: 30 165 30 781 30 4731 Fragments arranged by size: 4731 781 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 1186 Size: 856 4911 BseYI C'CCAG_C Cuts at: 4970 5466 4970 Size: 496 5271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5194 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3217 3978 5006 1540 Size: 275 186 1216 761 1028 2301 Fragments arranged by size: 2301 1216 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2709 3828 3913 4984 2709 Size: 1119 85 1071 3492 Fragments arranged by size: 3492 1119 1071 85 BsmI GAATG_Cn' Cuts at: 3278 3371 3278 Size: 93 5674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2709 3086 3828 3913 4984 1391 Size: 399 571 348 377 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 571 399 377 348 85 BspEI T'CCGG_A Cuts at: 2189 5430 2189 Size: 3241 2526 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3122 844 Size: 2278 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2047 2943 3654 1394 Size: 429 224 896 711 3507 Fragments arranged by size: 3507 896 711 429 224 BsrDI GCAATG_nn' Cuts at: 65 1248 2644 3033 4262 4436 65 Size: 1183 1396 389 1229 174 1396 Fragments arranged by size: 1396 1396 1229 1183 389 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2430 4411 4598 1700 Size: 110 620 1981 187 2869 Fragments arranged by size: 2869 1981 620 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 2738 3827 4839 2738 Size: 1089 1012 3666 Fragments arranged by size: 3666 1089 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 2180 2180 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2640 2821 3097 3464 3849 3866 5318 5404 Size: 668 181 276 367 385 17 1452 86 Cuts at: 5404 5416 5762 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 668 385 367 346 276 181 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2265 513 Size: 552 939 261 4015 Fragments arranged by size: 4015 939 552 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 3283 4077 4097 3283 Size: 794 20 4953 Fragments arranged by size: 4953 794 20 ClaI AT'CG_AT Cuts at: 1554 2787 1554 Size: 1233 4534 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2664 3214 4103 Size: 54 1425 50 275 18 834 550 889 Cuts at: 4103 5732 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 834 550 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3214 1537 Size: 275 1402 4090 Fragments arranged by size: 4090 1402 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2759 2810 3697 1353 Size: 1406 51 887 3423 Fragments arranged by size: 3423 1406 887 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2148 2578 3830 4427 5214 5482 1239 Size: 393 516 430 1252 597 787 268 1524 Fragments arranged by size: 1524 1252 787 597 516 430 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 5767 EcoO109I rG'GnC_Cy Cuts at: 2882 2882 Size: 5767 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 2094 4914 1678 Size: 416 2820 2531 Fragments arranged by size: 2820 2531 416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 4408 4440 4482 Size: 32 135 32 599 32 2205 32 42 Cuts at: 4482 4514 1373 Size: 32 2626 Fragments arranged by size: 2626 2205 599 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2321 2590 4881 5065 1305 Size: 1016 269 2291 184 2007 Fragments arranged by size: 2291 2007 1016 269 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3945 Fragments arranged by size: 3945 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2150 2178 3002 3725 4084 4243 518 Size: 648 984 28 824 723 359 159 2042 Fragments arranged by size: 2042 984 824 723 648 359 159 28 MspA1I CmG'CkG Cuts at: 1606 2691 3183 3860 5008 5253 1606 Size: 1085 492 677 1148 245 2120 Fragments arranged by size: 2120 1148 1085 677 492 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2265 513 Size: 552 939 261 4015 Fragments arranged by size: 4015 939 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 2737 3125 4670 1132 Size: 97 1508 388 1545 2229 Fragments arranged by size: 2229 1545 1508 388 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 4666 4666 Size: 5767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2245 1108 Size: 1137 4630 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3836 3868 5349 5381 3836 Size: 32 1481 32 4222 Fragments arranged by size: 4222 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 3337 3337 Size: 5767 PspXI vC'TCGA_Gb Cuts at: 2042 2042 Size: 5767 PstI C_TGCA'G Cuts at: 830 2371 3119 830 Size: 1541 748 3478 Fragments arranged by size: 3478 1541 748 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SapI GCTCTTCn'nnn_ Cuts at: 2759 2759 Size: 5767 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SexAI A'CCwGG_T Cuts at: 2700 2700 Size: 5767 SfcI C'TryA_G Cuts at: 826 1045 2224 2367 3115 4250 4931 5122 826 Size: 219 1179 143 748 1135 681 191 1471 Fragments arranged by size: 1471 1179 1135 748 681 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 2438 3094 1872 Size: 566 656 4545 Fragments arranged by size: 4545 656 566 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 3871 4772 5034 820 Size: 197 926 99 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 99 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 2737 3125 1132 Size: 1605 388 3774 Fragments arranged by size: 3774 1605 388 SspI AAT'ATT Cuts at: 5 52 3691 5 Size: 47 3639 2081 Fragments arranged by size: 3639 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5767 StyI C'CwwG_G Cuts at: 513 1065 2004 2265 3206 513 Size: 552 939 261 941 3074 Fragments arranged by size: 3074 941 939 552 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2962 3794 3811 3964 4149 4594 325 Size: 1255 1382 832 17 153 185 445 1498 Fragments arranged by size: 1498 1382 1255 832 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2012 2208 4013 Size: 274 80 33 51 1443 35 196 1805 Cuts at: 4013 5725 96 Size: 1712 138 Fragments arranged by size: 1805 1712 1443 274 196 138 80 51 35 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2184 2216 5727 5759 2184 Size: 32 3511 32 2192 Fragments arranged by size: 3511 2192 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3017 1316 Size: 1701 4066 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2272 2470 2272 Size: 198 5569 XhoI C'TCGA_G Cuts at: 1943 2042 1943 Size: 99 5668 XmaI C'CCGG_G Cuts at: 1870 2436 3092 1870 Size: 566 656 4545 Fragments arranged by size: 4545 656 566 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2608 2659 3575 276 Size: 53 83 186 936 1074 51 916 2468 Fragments arranged by size: 2468 1074 936 916 186 83 53 51 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI