Restriction map for
pFN21AB3635
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MaeIII
MamI MfeI MluI MluNI MscI
MslI MspA1I MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccII 101 CGCG CG^CG
AccIII 185 TCCGGA T^CCGGA
AlwNI 299 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 323 GGATCC G^GATCC
BfaI 158 CTAG C^TAG
BsaWI 185 TCCGGA [AT]^CCGG[AT]
BseAI 185 TCCGGA T^CCGGA
BseRI 478 GAGGAG GAGGAG(10/8)
BsgI 213 CTGCAC GTGCAG(16/14)
BspDI 337 ATCGAT AT^CGAT
BspEI 185 TCCGGA T^CCGGA
BsrI 304 ACTGG ACTGG(1/-1)
BstUI 101 CGCG CG^CG
BstXI 110 CCAAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 102 GCGAGC GC[ACGT]^[ACGT]GC
ClaI 337 ATCGAT AT^CGAT
DdeI 297 CTCAG C^T[ACGT]AG
HapII 186 CCGG C^CGG
HgaI 99 GACGC GACGC(5/10)
HinfI 467 GAATC G^A[ACGT]TC
HpaII 186 CCGG C^CGG
HphI 16 GGTGA GGTGA(8/7)
MaeI 158 CTAG C^TAG
MboII 238 GAAGA GAAGA(8/7)
MroI 185 TCCGGA T^CCGGA
MseI 280 TTAA T^TAA
MspI 186 CCGG C^CGG
MvnI 101 CGCG CG^CG
TfiI 467 GAATC G^A[AT]TC
Tru9I 280 TTAA T^TAA
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# TWO CUT ENZYMES ( 17 enzymes) #
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AluI 125 AGCT AG^CT
156 AGCT
BsaJI 110 CCAAGG C^C[ACGT][ACGT]GG
230 CCAAGG
BsmAI 89 GAGAC GTCTC(1/5)
62 GTCTC
BstYI 172 AGATCC [GA]^GATC[CT]
323 GGATCC
Cfr13I 227 GGGCC G^G[ACGT]CC
455 GGGCC
EcoT14I 110 CCAAGG C^C[AT][AT]GG
230 CCAAGG
FauI 36 GCGGG CCCGC(4/6)
267 GCGGG
MflI 172 AGATCC [GA]^GATC[CT]
323 GGATCC
NlaIII 33 CATG CATG^
486 CATG
PflMI 361 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCATGCGCTGG
Sau96I 227 GGGCC G^G[ACGT]CC
455 GGGCC
StyI 110 CCAAGG C^C[AT][AT]GG
230 CCAAGG
TaqI 222 TCGA T^CGA
338 TCGA
TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^
211 CACTG
TthHB8I 222 TCGA T^CGA
338 TCGA
Van91I 361 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
485 CCATGCGCTGG
XhoII 172 AGATCC [GA]^GATC[CT]
323 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AciI AlwI BbvI BsiYI BslI
BssKI BstF5I BstNI CfoI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I ItaI MboI MnlI MvaI
MwoI NdeII NlaIV Sau3AI ScrFI
SfaNI TseI