(Circular) (Six-base) MAPSORT of: pFN21AB3544.seq Check: 8546 from: 1 to: 5029 pFN21AB3544 5029 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2005 2005 Size: 5029 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2839 278 Size: 53 83 186 936 1303 2468 Fragments arranged by size: 2468 1303 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2372 1937 Size: 435 4594 Acc65I G'GTAC_C Cuts at: 2350 2350 Size: 5029 AclI AA'CG_TT Cuts at: 1563 3156 3529 1563 Size: 1593 373 3063 Fragments arranged by size: 3063 1593 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3092 4476 4744 1632 Size: 1460 1384 268 1917 Fragments arranged by size: 1917 1460 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4832 AflIII A'CryG_T Cuts at: 1203 3928 4905 1203 Size: 2725 977 1327 Fragments arranged by size: 2725 1327 977 AgeI A'CCGG_T Cuts at: 3860 3860 Size: 5029 AhdI GACnn_n'nnGTC Cuts at: 3758 3758 Size: 5029 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5029 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5029 AlwNI CAG_nnn'CTG Cuts at: 2772 4344 2772 Size: 1572 3457 ApaLI G'TGCA_C Cuts at: 2078 3086 4242 2078 Size: 1008 1156 2865 Fragments arranged by size: 2865 1156 1008 ApoI r'AATT_y Cuts at: 1493 2111 2338 2564 2716 4825 1493 Size: 618 227 226 152 2109 1697 Fragments arranged by size: 2109 1697 618 227 226 152 AseI AT'TA_AT Cuts at: 160 1032 3583 160 Size: 872 2551 1606 Fragments arranged by size: 2551 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5029 AvaI C'yCGr_G Cuts at: 1870 1943 2124 2354 1870 Size: 73 181 230 4545 Fragments arranged by size: 4545 230 181 73 BamHI G'GATC_C Cuts at: 2359 2726 2359 Size: 367 4662 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2255 2265 2350 3805 618 Size: 325 217 627 468 10 85 1455 1842 Fragments arranged by size: 1842 1455 627 468 325 217 85 10 BanII G_rGCy'C Cuts at: 1391 2348 1391 Size: 957 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2293 928 Size: 971 394 3664 Fragments arranged by size: 3664 971 394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3220 3254 1903 Size: 34 1283 34 3678 Fragments arranged by size: 3678 1283 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2921 4131 2921 Size: 1210 3819 BclI T'GATC_A Cuts at: 1548 1548 Size: 5029 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3640 136 Size: 107 122 71 631 605 1968 1525 Fragments arranged by size: 1968 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5024 5024 Size: 5029 BlpI GC'TnA_GC Cuts at: 2446 2446 Size: 5029 Bme1580I G_kGCm'C Cuts at: 1790 2082 2268 3090 4246 1790 Size: 292 186 822 1156 2573 Fragments arranged by size: 2573 1156 822 292 186 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5029 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2271 2760 3718 Size: 416 53 83 399 24 854 489 958 Cuts at: 3718 442 Size: 1753 Fragments arranged by size: 1753 958 854 489 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5029 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2223 3689 1239 Size: 984 1466 2579 Fragments arranged by size: 2579 1466 984 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3154 4019 4317 3154 Size: 865 298 3866 Fragments arranged by size: 3866 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3692 882 Size: 617 2193 2219 Fragments arranged by size: 2219 2193 617 BsaAI yAC'GTr Cuts at: 493 1204 2820 493 Size: 711 1616 2702 Fragments arranged by size: 2702 1616 711 BsaBI GATnn'nnATC Cuts at: 5023 5023 Size: 5029 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2836 3218 3853 275 Size: 53 83 186 936 1303 382 635 1451 Fragments arranged by size: 1451 1303 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3462 3860 4134 4281 4692 3462 Size: 398 274 147 411 3799 Fragments arranged by size: 3799 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2001 2031 2221 2251 1178 Size: 30 165 30 598 30 190 30 3956 Fragments arranged by size: 3956 598 190 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5029 BseYI C'CCAG_C Cuts at: 2181 4232 4728 2181 Size: 2051 496 2482 Fragments arranged by size: 2482 2051 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2033 1163 Size: 573 297 4159 Fragments arranged by size: 4159 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2479 3240 4268 1540 Size: 275 186 478 761 1028 2301 Fragments arranged by size: 2301 1028 761 478 275 186 BsiHKAI G_wGCw'C Cuts at: 2082 3090 3175 4246 2082 Size: 1008 85 1071 2865 Fragments arranged by size: 2865 1071 1008 85 BsmI GAATG_Cn' Cuts at: 2540 2633 2540 Size: 93 4936 BsmBI CGTCTCn'nnnn_ Cuts at: 2031 2031 Size: 5029 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2268 2348 3090 3175 4246 1391 Size: 399 292 186 80 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 292 186 85 80 BspEI T'CCGG_A Cuts at: 4692 4692 Size: 5029 BspHI T'CATG_A Cuts at: 2918 4648 2918 Size: 1730 3299 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 2078 2384 844 Size: 1161 73 306 3489 Fragments arranged by size: 3489 1161 306 73 BsrBI CCG'CTC Cuts at: 1394 1823 2916 1394 Size: 429 1093 3507 Fragments arranged by size: 3507 1093 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3524 3698 65 Size: 1183 2276 174 1396 Fragments arranged by size: 2276 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3673 3860 1700 Size: 110 1863 187 2869 Fragments arranged by size: 2869 1863 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5029 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5029 BssSI C'ACGA_G Cuts at: 3089 4101 3089 Size: 1012 4017 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5029 BstBI TT'CG_AA Cuts at: 3833 3833 Size: 5029 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5029 BstYI r'GATC_y Cuts at: 1972 2359 2726 3111 3128 4580 4666 4678 5024 Size: 387 367 385 17 1452 86 12 346 Cuts at: 5024 1972 Size: 1977 Fragments arranged by size: 1977 1452 387 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3538 Fragments arranged by size: 3538 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4290 Fragments arranged by size: 4290 683 56 BtsI GCAGTG_nn' Cuts at: 2545 3339 3359 2545 Size: 794 20 4215 Fragments arranged by size: 4215 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5029 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4994 DraI TTT'AAA Cuts at: 2333 2333 Size: 5029 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4036 809 Size: 507 2720 1802 Fragments arranged by size: 2720 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2476 3365 Size: 54 1425 50 275 18 232 414 889 Cuts at: 3365 4994 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 414 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2476 1537 Size: 275 664 4090 Fragments arranged by size: 4090 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2959 1353 Size: 1606 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3616 3990 4136 387 Size: 1064 138 2027 374 146 1280 Fragments arranged by size: 2027 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 3092 3689 4476 4744 1239 Size: 393 591 869 597 787 268 1524 Fragments arranged by size: 1524 869 787 597 591 393 268 EcoRI G'AATT_C Cuts at: 2338 2716 2338 Size: 378 4651 FspI TGC'GCA Cuts at: 3535 3535 Size: 5029 HaeII r_GCGC'y Cuts at: 1678 4176 1678 Size: 2498 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2197 2221 2229 2253 3670 Size: 32 135 32 625 24 8 24 1417 Cuts at: 3670 3702 3744 3776 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 625 135 42 32 32 32 32 24 24 8 HincII GTy'rAC Cuts at: 669 1657 1938 2373 2619 669 Size: 988 281 435 246 3079 Fragments arranged by size: 3079 988 435 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5029 HpaI GTT'AAC Cuts at: 2619 2619 Size: 5029 KpnI G_GTAC'C Cuts at: 2354 2354 Size: 5029 MfeI C'AATT_G Cuts at: 2628 2628 Size: 5029 MluI A'CGCG_T Cuts at: 4905 4905 Size: 5029 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4143 4327 1305 Size: 2838 184 2007 Fragments arranged by size: 2838 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3207 Fragments arranged by size: 3207 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2090 2987 3346 3505 518 Size: 648 843 81 897 359 159 2042 Fragments arranged by size: 2042 897 843 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 2445 3122 4270 4515 1606 Size: 839 677 1148 245 2120 Fragments arranged by size: 2120 1148 839 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3538 Fragments arranged by size: 3538 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5029 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5029 NotI GC'GGCC_GC Cuts at: 2476 2476 Size: 5029 NspI r_CATG'y Cuts at: 1132 1229 2291 2387 3932 1132 Size: 97 1062 96 1545 2229 Fragments arranged by size: 2229 1545 1062 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5029 PciI A'CATG_T Cuts at: 3928 3928 Size: 5029 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5029 PmeI GTTT'AAAC Cuts at: 2333 2333 Size: 5029 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3098 3130 4611 4643 3098 Size: 32 1481 32 3484 Fragments arranged by size: 3484 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5029 PsiI TTA'TAA Cuts at: 2599 2599 Size: 5029 PstI C_TGCA'G Cuts at: 830 2381 830 Size: 1551 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5029 SalI G'TCGA_C Cuts at: 1936 2371 1936 Size: 435 4594 SbfI CC_TGCA'GG Cuts at: 2381 2381 Size: 5029 ScaI AGT'ACT Cuts at: 3277 4989 3277 Size: 1712 3317 SfcI C'TryA_G Cuts at: 826 1045 2377 3512 4193 4384 826 Size: 219 1332 1135 681 191 1471 Fragments arranged by size: 1471 1332 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5029 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5029 SmaI CCC'GGG Cuts at: 1872 2126 2356 1872 Size: 254 230 4545 Fragments arranged by size: 4545 254 230 SmlI C'TyrA_G Cuts at: 820 1017 1943 3133 4034 4296 820 Size: 197 926 1190 901 262 1553 Fragments arranged by size: 1553 1190 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2820 493 Size: 2327 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5029 SphI G_CATG'C Cuts at: 1132 2387 1132 Size: 1255 3774 SspI AAT'ATT Cuts at: 5 52 2953 5 Size: 47 2901 2081 Fragments arranged by size: 2901 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5029 StyI C'CwwG_G Cuts at: 513 1065 2004 2468 513 Size: 552 939 464 3074 Fragments arranged by size: 3074 939 552 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2283 3056 3073 3226 3411 3856 325 Size: 1255 703 773 17 153 185 445 1498 Fragments arranged by size: 1498 1255 773 703 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3275 4987 96 Size: 274 80 33 51 1443 1298 1712 138 Fragments arranged by size: 1712 1443 1298 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2001 2033 4989 5021 2001 Size: 32 2956 32 2009 Fragments arranged by size: 2956 2009 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5029 XbaI T'CTAG_A Cuts at: 2365 2365 Size: 5029 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5029 XmaI C'CCGG_G Cuts at: 1870 2124 2354 1870 Size: 254 230 4545 Fragments arranged by size: 4545 254 230 XmnI GAAnn'nnTTC Cuts at: 3158 3158 Size: 5029 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2837 276 Size: 53 83 186 936 1303 2468 Fragments arranged by size: 2468 1303 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI