Restriction map for pFN21AB3448
 (Circular) (Six-base) MAPSORT of: pFN21AB3448.seq  Check: 7885  from: 1  to: 5593



pFN21AB3448  5593 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2441    2441 
   Size:       5593
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3403     278 
   Size:         53      83     186     936    1867    2468

  Fragments arranged by size: 

                2468    1867     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2936    1937 
   Size:        999    4594
 
Acc65I G'GTAC_C

Cuts at:   2914    2914 
   Size:       5593
 
AclI AA'CG_TT

Cuts at:   1563    3720    4093    1563 
   Size:       2157     373    3063

  Fragments arranged by size: 

                3063    2157     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2513    3656    5040    5308    1632 
   Size:        881    1143    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1143     881     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5396
 
AflIII A'CryG_T

Cuts at:   1203    4492    5469    1203 
   Size:       3289     977    1327

  Fragments arranged by size: 

                3289    1327     977
 
AgeI A'CCGG_T

Cuts at:   4424    4424 
   Size:       5593
 
AhdI GACnn_n'nnGTC

Cuts at:   4322    4322 
   Size:       5593
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5593
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5593
 
AlwNI CAG_nnn'CTG

Cuts at:   3336    4908    3336 
   Size:       1572    4021
 
ApaLI G'TGCA_C

Cuts at:   3650    4806    3650 
   Size:       1156    4437
 
ApoI r'AATT_y

Cuts at:   1493    2060    2570    2902    3128    3280    5389    1493 
   Size:        567     510     332     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     567     510     332     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4147     160 
   Size:        872    3115    1606

  Fragments arranged by size: 

                3115    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5593
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2918    1870 
   Size:         73     975    4545

  Fragments arranged by size: 

                4545     975      73
 
BamHI G'GATC_C

Cuts at:   2923    3290    2923 
   Size:        367    5226
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2423    2486    2914    4369     618 
   Size:        325     217     627     636      63     428    1455    1842

  Fragments arranged by size: 

                1842    1455     636     627     428     325     217      63
            
 
BanII G_rGCy'C

Cuts at:   1391    2872    2912    1391 
   Size:       1481      40    4072

  Fragments arranged by size: 

                4072    1481      40
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2074     928 
   Size:        971     175    4447

  Fragments arranged by size: 

                4447     971     175
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3784    3818    1903 
   Size:         34    1847      34    3678

  Fragments arranged by size: 

                3678    1847      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3485    4695    3485 
   Size:       1210    4383
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5593
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4204     136 
   Size:        107     122      71     631     605    2532    1525

  Fragments arranged by size: 

                2532    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2355    5588    2355 
   Size:       3233    2360
 
BlpI GC'TnA_GC

Cuts at:   3010    3010 
   Size:       5593
 
Bme1580I G_kGCm'C

Cuts at:   1790    2491    3654    4810    1790 
   Size:        701    1163    1156    2573

  Fragments arranged by size: 

                2573    1163    1156     701
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5593
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3324    4282     442 
   Size:        416      53      83     399      24    1907     958    1753

  Fragments arranged by size: 

                1907    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5593
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2685    4253    1239 
   Size:       1446    1568    2579

  Fragments arranged by size: 

                2579    1568    1446
 
Bpu10I CC'TnA_GC

Cuts at:   2871    2871 
   Size:       5593
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3718    4583    4881    3718 
   Size:        865     298    4430

  Fragments arranged by size: 

                4430     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4256     882 
   Size:        617    2757    2219

  Fragments arranged by size: 

                2757    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3384     493 
   Size:        711    2180    2702

  Fragments arranged by size: 

                2702    2180     711
 
BsaBI GATnn'nnATC

Cuts at:   5587    5587 
   Size:       5593
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3400    3782    4417     275 
   Size:         53      83     186     936    1867     382     635    1451

  Fragments arranged by size: 

                1867    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4026    4424    4698    4845    5256    4026 
   Size:        398     274     147     411    4363

  Fragments arranged by size: 

                4363     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2702    2732    1178 
   Size:         30     165      30    1299      30    4039

  Fragments arranged by size: 

                4039    1299     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2726    1186 
   Size:       1540    4053
 
BseYI C'CCAG_C

Cuts at:   4796    5292    4796 
   Size:        496    5097
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5020
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3043    3804    4832    1540 
   Size:        275     186    1042     761    1028    2301

  Fragments arranged by size: 

                2301    1042    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2872    3654    3739    4810    2872 
   Size:        782      85    1071    3655

  Fragments arranged by size: 

                3655    1071     782      85
 
BsmI GAATG_Cn'

Cuts at:   3104    3197    3104 
   Size:         93    5500
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2491    2872    2912    3654    3739    4810    1391 
   Size:        399     701     381      40     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     701     399     381      85      40
            
 
BspEI T'CCGG_A

Cuts at:   5256    5256 
   Size:       5593
 
BspHI T'CATG_A

Cuts at:   3482    5212    3482 
   Size:       1730    3863
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2441    2948     844 
   Size:       1597     507    3489

  Fragments arranged by size: 

                3489    1597     507
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2878    3480    1394 
   Size:        429    1055     602    3507

  Fragments arranged by size: 

                3507    1055     602     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4088    4262      65 
   Size:       1183    2840     174    1396

  Fragments arranged by size: 

                2840    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4237    4424    1700 
   Size:        110    2427     187    2869

  Fragments arranged by size: 

                2869    2427     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5593
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5593
 
BssSI C'ACGA_G

Cuts at:   3653    4665    3653 
   Size:       1012    4581
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2240    2453     794 
   Size:       1446     213    3934

  Fragments arranged by size: 

                3934    1446     213
 
BstBI TT'CG_AA

Cuts at:   4397    4397 
   Size:       5593
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5593
 
BstYI r'GATC_y

Cuts at:   1972    2355    2923    3290    3675    3692    5144    5230    5242 
   Size:        383     568     367     385      17    1452      86      12

Cuts at:   5242    5588    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     568     385     383     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4102

  Fragments arranged by size: 

                4102     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4854

  Fragments arranged by size: 

                4854     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3109    3903    3923    3109 
   Size:        794      20    4779

  Fragments arranged by size: 

                4779     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5593
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5558
 
DraI TTT'AAA

Cuts at:   2897    2897 
   Size:       5593
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4600     809 
   Size:        507    3284    1802

  Fragments arranged by size: 

                3284    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3040    3929    5558 
   Size:         54    1425      50     275      18    1210     889    1629

Cuts at:   5558       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1210     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3040    1537 
   Size:        275    1228    4090

  Fragments arranged by size: 

                4090    1228     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2786    3523    1353 
   Size:       1433     737    3423

  Fragments arranged by size: 

                3423    1433     737
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4180    4554    4700     387 
   Size:       1064     138    2591     374     146    1280

  Fragments arranged by size: 

                2591    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2870    2870 
   Size:       5593
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2513    2685    3656    4253    5040    5308    1239 
   Size:        393     881     172     971     597     787     268    1524

  Fragments arranged by size: 

                1524     971     881     787     597     393     268     172
            
 
EcoRI G'AATT_C

Cuts at:   2902    3280    2902 
   Size:        378    5215
 
EcoRV GAT'ATC

Cuts at:   2182    2182 
   Size:       5593
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2056    2875    2056 
   Size:        819    4774
 
FspI TGC'GCA

Cuts at:   2233    4099    2233 
   Size:       1866    3727
 
HaeII r_GCGC'y

Cuts at:   1678    4740    1678 
   Size:       3062    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    4234    4266    4308    4340    1373 
   Size:         32     135      32    2662      32      42      32    2626

  Fragments arranged by size: 

                2662    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2937    3183     669 
   Size:        988     281     999     246    3079

  Fragments arranged by size: 

                3079     999     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2857     748 
   Size:       2109    3484
 
HpaI GTT'AAC

Cuts at:   3183    3183 
   Size:       5593
 
KpnI G_GTAC'C

Cuts at:   2918    2918 
   Size:       5593
 
MfeI C'AATT_G

Cuts at:   3192    3192 
   Size:       5593
 
MluI A'CGCG_T

Cuts at:   5469    5469 
   Size:       5593
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4707    4891    1305 
   Size:       3402     184    2007

  Fragments arranged by size: 

                3402    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3771

  Fragments arranged by size: 

                3771    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3551    3910    4069     518 
   Size:        648    2385     359     159    2042

  Fragments arranged by size: 

                2385    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3009    3686    4834    5079    1606 
   Size:       1403     677    1148     245    2120

  Fragments arranged by size: 

                2120    1403    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4102

  Fragments arranged by size: 

                4102     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5593
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5593
 
NotI GC'GGCC_GC

Cuts at:   3040    3040 
   Size:       5593
 
NspI r_CATG'y

Cuts at:   1132    1229    2629    2951    4496    1132 
   Size:         97    1400     322    1545    2229

  Fragments arranged by size: 

                2229    1545    1400     322      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5593
 
PciI A'CATG_T

Cuts at:   4492    4492 
   Size:       5593
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2261    1108 
   Size:       1153    4440
 
PmeI GTTT'AAAC

Cuts at:   2897    2897 
   Size:       5593
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3662    3694    5175    5207    3662 
   Size:         32    1481      32    4048

  Fragments arranged by size: 

                4048    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5593
 
PsiI TTA'TAA

Cuts at:   3163    3163 
   Size:       5593
 
PstI C_TGCA'G

Cuts at:    830    2296    2945     830 
   Size:       1466     649    3478

  Fragments arranged by size: 

                3478    1466     649
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5593
 
SacI G_AGCT'C

Cuts at:   2872    2872 
   Size:       5593
 
SalI G'TCGA_C

Cuts at:   1936    2935    1936 
   Size:        999    4594
 
SbfI CC_TGCA'GG

Cuts at:   2945    2945 
   Size:       5593
 
ScaI AGT'ACT

Cuts at:   3841    5553    3841 
   Size:       1712    3881
 
SfcI C'TryA_G

Cuts at:    826    1045    2292    2941    4076    4757    4948     826 
   Size:        219    1247     649    1135     681     191    1471

  Fragments arranged by size: 

                1471    1247    1135     681     649     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5593
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5593
 
SmaI CCC'GGG

Cuts at:   1872    2920    1872 
   Size:       1048    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3697    4598    4860     820 
   Size:        197     926    1754     901     262    1553

  Fragments arranged by size: 

                1754    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3384     493 
   Size:       2891    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5593
 
SphI G_CATG'C

Cuts at:   1132    2951    1132 
   Size:       1819    3774
 
SspI AAT'ATT

Cuts at:      5      52    3517       5 
   Size:         47    3465    2081

  Fragments arranged by size: 

                3465    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2036    2451    1517 
   Size:        519     415    4659

  Fragments arranged by size: 

                4659     519     415
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3032     513 
   Size:        552     939    1028    3074

  Fragments arranged by size: 

                3074    1028     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2158    3620    3637    3790    3975    4420     325 
   Size:       1255     578    1462      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1462    1255     578     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2303    2584    3839 
   Size:        274      80      33      51    1443     326     281    1255

Cuts at:   3839    5551      96 
   Size:       1712     138

  Fragments arranged by size: 

                1712    1443    1255     326     281     274     138      80
                  51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5553    5585    5553 
   Size:         32    5561
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5593
 
XbaI T'CTAG_A

Cuts at:   2929    2929 
   Size:       5593
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5593
 
XmaI C'CCGG_G

Cuts at:   1870    2918    1870 
   Size:       1048    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2669    3722    2669 
   Size:       1053    4540
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3401     276 
   Size:         53      83     186     936    1867    2468

  Fragments arranged by size: 

                2468    1867     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI EcoO109I     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI     XcmI
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