Restriction map for
pFN21AB3441
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvaI
AvrII BamHI BbsI BbvCI BcgI
BciVI BclI BfaI BlnI BlpI
Bpu1102I BpuAI BseAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DsaI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT14I FbaI FseI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MfeI MluI
MroI MunI NcoI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tsp509I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AfaI 864 GTAC GT^AC
AflII 40 CTTAAG C^TTAAG
AflIII 493 ACGTGT A^C[GA][CT]GT
AhdI 590 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 815 GGATC GGATC(4/5)
AspEI 590 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 778 GAGCTC G[AT]GC[AT]^C
AspI 984 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 134 TGCGCA TGC^GCA
BanII 778 GAGCTC G[GA]GC[CT]^C
BbeI 845 GGCGCC GGCGC^C
BbrPI 492 CACGTG CAC^GTG
BcnI 909 CCGGG CC^[GC]GG
BfrI 40 CTTAAG C^TTAAG
BglI 904 GCCGACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 492 CACGTG [CT]AC^GT[GA]
BsaBI 457 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 514 GAGACC GGTCTC(1/5)
BsaWI 541 ACCGGA [AT]^CCGG[AT]
BsgI 74 GTGCAG GTGCAG(16/14)
BsiEI 906 CGACCG CG[GA][CT]^CG
BsiHKAI 778 GAGCTC G[AT]GC[AT]^C
BsrBI 840 GAGCGG CCGCTC(-3/-3)
BsrDI 713 GCAATG GCAATG(2/0)
BstEII 430 GGTTACC G^GT[ACGT]ACC
BstPI 430 GGTTACC G^GT[ACGT]ACC
DraII 988 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 489 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 934 GACAACCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 590 GACTGTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 988 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 430 GGTTACC G^GT[ACGT]ACC
EcoT22I 716 ATGCAT ATGCA^T
FspI 134 TGCGCA TGC^GCA
HaeII 845 GGCGCC [GA]GCGC^[CT]
KasI 845 GGCGCC G^GCGCC
MamI 457 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 122 GAAGA GAAGA(8/7)
MseI 41 TTAA T^TAA
MslI 972 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 945 CAGCTG C[AC]G^C[GT]G
NaeI 653 GCCGGC GCC^GGC
NarI 845 GGCGCC GG^CGCC
NciI 909 CCGGG CC^[GC]GG
NdeI 311 CATATG CA^TATG
NgoMIV 653 GCCGGC G^CCGGC
NsiI 716 ATGCAT ATGCA^T
PflFI 984 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 492 CACGTG CAC^GTG
PmlI 492 CACGTG CAC^GTG
PpuMI 988 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 747 CTGCAG CTGCA^G
PvuII 945 CAGCTG CAG^CTG
RsaI 864 GTAC GT^AC
SacI 778 GAGCTC GAGCT^C
SbfI 746 CCTGCAGG CCTGCA^GG
SfcI 747 CTGCAG C^T[GA][CT]AG
SfoI 845 GGCGCC GGC^GCC
Sse8387I 746 CCTGCAGG CCTGCA^GG
Tru9I 41 TTAA T^TAA
Tth111I 984 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 400 CGCG CG^CG
940 CGCG
AvaII 267 GGTCC G^G[AT]CC
989 GGTCC
BalI 164 TGGCCA TGG^CCA
669 TGGCCA
BanI 158 GGCACC G^G[CT][GA]CC
845 GGCGCC
BbvI 443 GCTGC GCAGC(8/12)
947 GCTGC
BglII 170 AGATCT A^GATCT
1010 AGATCT
BsmBI 597 CGTCTC CGTCTC(1/5)
56 GAGACG
BsmFI 245 GGGAC GGGAC(10/14)
506 GGGAC
BspMI 745 ACCTGC ACCTGC(4/8)
913 GCAGGT
BsrFI 517 ACCGGC [GA]^CCGG[CT]
653 GCCGGC
BstUI 400 CGCG CG^CG
940 CGCG
BstYI 170 AGATCT [GA]^GATC[CT]
1010 AGATCT
Cfr10I 517 ACCGGC [GA]^CCGG[CT]
653 GCCGGC
Cfr13I 267 GGTCC G^G[ACGT]CC
989 GGTCC
DdeI 92 CTCAG C^T[ACGT]AG
732 CTCAG
Fnu4HI 443 GCTGC GC^[ACGT]GC
947 GCTGC
HphI 473 TCACC GGTGA(8/7)
818 TCACC
ItaI 443 GCTGC GC^[ACGT]GC
947 GCTGC
MflI 170 AGATCT [GA]^GATC[CT]
1010 AGATCT
MluNI 164 TGGCCA TGG^CCA
669 TGGCCA
MscI 164 TGGCCA TGG^CCA
669 TGGCCA
MvnI 400 CGCG CG^CG
940 CGCG
NspI 70 GCATGT [GA]CATG^[CT]
647 GCATGT
PflMI 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCAAACTCTGG
Sau96I 267 GGTCC G^G[ACGT]CC
989 GGTCC
SmlI 40 CTTAAG C^T[CT][GA]AG
1006 CTCAAG
TseI 443 GCTGC G^C[AT]GC
947 GCTGC
Tsp45I 472 GTCAC ^GT[GC]AC
958 GTGAC
Van91I 179 CCATGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
623 CCAAACTCTGG
XhoII 170 AGATCT [GA]^GATC[CT]
1010 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcyI AluI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BspHI BsrI BssKI
BssSI BstF5I BstNI Cac8I CfoI
DpnII EaeI EcoRII FauI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII MaeII MaeIII
MboI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RcaI
Sau3AI ScrFI SfaNI TaqI TfiI
TspRI TthHB8I