Restriction map for
pFN21AB3441
(Linear) (Six-base) MAPSORT of: KIBB3441.seq Check: 5051 from: 1 to: 1020
KIBB3441.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 754 904 1020
Size: 754 150 116
Fragments arranged by size:
754 150 116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 602 1020
Size: 602 418
AflII C'TTAA_G
Cuts at: 0 40 1020
Size: 40 980
AflIII A'CryG_T
Cuts at: 0 493 1020
Size: 493 527
AhdI GACnn_n'nnGTC
Cuts at: 0 595 1020
Size: 595 425
BanI G'GyrC_C
Cuts at: 0 158 845 1020
Size: 158 687 175
Fragments arranged by size:
687 175 158
BanII G_rGCy'C
Cuts at: 0 782 1020
Size: 782 238
BbeI G_GCGC'C
Cuts at: 0 849 1020
Size: 849 171
BfrBI ATG'CAT
Cuts at: 0 718 1020
Size: 718 302
BglI GCCn_nnn'nGGC
Cuts at: 0 910 1020
Size: 910 110
BglII A'GATC_T
Cuts at: 0 170 1010 1020
Size: 170 840 10
Fragments arranged by size:
840 170 10
Bme1580I G_kGCm'C
Cuts at: 0 161 193 501 1020
Size: 161 32 308 519
Fragments arranged by size:
519 308 161 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 109 195 1020
Size: 109 86 825
Fragments arranged by size:
825 109 86
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 587 1020
Size: 587 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 503 572 1020
Size: 183 320 69 448
Fragments arranged by size:
448 320 183 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1020
Size: 991 29
BsaI GGTCTCn'nnnn_
Cuts at: 0 508 1020
Size: 508 512
BsaAI yAC'GTr
Cuts at: 0 494 1020
Size: 494 526
BsaBI GATnn'nnATC
Cuts at: 0 461 1020
Size: 461 559
BsaHI Gr'CG_yC
Cuts at: 0 59 740 846 1020
Size: 59 681 106 174
Fragments arranged by size:
681 174 106 59
BsaWI w'CCGG_w
Cuts at: 0 541 1020
Size: 541 479
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 346 376 1020
Size: 346 30 644
Fragments arranged by size:
644 346 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 279 841 1020
Size: 156 123 562 179
Fragments arranged by size:
562 179 156 123
BseYI C'CCAG_C
Cuts at: 0 859 925 1020
Size: 859 66 95
Fragments arranged by size:
859 95 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 95 1020
Size: 95 925
BsiEI CG_ry'CG
Cuts at: 0 909 1020
Size: 909 111
BsiHKAI G_wGCw'C
Cuts at: 0 782 1020
Size: 782 238
BsmBI CGTCTCn'nnnn_
Cuts at: 0 50 603 1020
Size: 50 553 417
Fragments arranged by size:
553 417 50
Bsp1286I G_dGCh'C
Cuts at: 0 161 193 501 782 1020
Size: 161 32 308 281 238
Fragments arranged by size:
308 281 238 161 32
BspHI T'CATG_A
Cuts at: 0 299 560 782 1020
Size: 299 261 222 238
Fragments arranged by size:
299 261 238 222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 754 904 1020
Size: 754 150 116
Fragments arranged by size:
754 150 116
BsrBI CCG'CTC
Cuts at: 0 842 1020
Size: 842 178
BsrDI GCAATG_nn'
Cuts at: 0 720 1020
Size: 720 300
BsrFI r'CCGG_y
Cuts at: 0 517 653 1020
Size: 517 136 367
Fragments arranged by size:
517 367 136
BssSI C'ACGA_G
Cuts at: 0 138 383 673 1020
Size: 138 245 290 347
Fragments arranged by size:
347 290 245 138
BstEII G'GTnAC_C
Cuts at: 0 430 1020
Size: 430 590
BstYI r'GATC_y
Cuts at: 0 170 1010 1020
Size: 170 840 10
Fragments arranged by size:
840 170 10
BtsI GCAGTG_nn'
Cuts at: 0 148 1020
Size: 148 872
DraIII CAC_nnn'GTG
Cuts at: 0 494 1020
Size: 494 526
DrdI GACnn_nn'nnGTC
Cuts at: 0 940 1020
Size: 940 80
EaeI y'GGCC_r
Cuts at: 0 164 519 669 1020
Size: 164 355 150 351
Fragments arranged by size:
355 351 164 150
EcoICRI GAG'CTC
Cuts at: 0 780 1020
Size: 780 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 503 572 602 1020
Size: 183 320 69 30 418
Fragments arranged by size:
418 320 183 69 30
EcoO109I rG'GnC_Cy
Cuts at: 0 989 1020
Size: 989 31
FspI TGC'GCA
Cuts at: 0 136 1020
Size: 136 884
FspAI rTGC'GCAy
Cuts at: 0 136 1020
Size: 136 884
HaeII r_GCGC'y
Cuts at: 0 849 1020
Size: 849 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 581 613 1020
Size: 581 32 407
Fragments arranged by size:
581 407 32
KasI G'GCGC_C
Cuts at: 0 845 1020
Size: 845 175
MscI TGG'CCA
Cuts at: 0 166 671 1020
Size: 166 505 349
Fragments arranged by size:
505 349 166
MslI CAynn'nnrTG
Cuts at: 0 976 1020
Size: 976 44
MspA1I CmG'CkG
Cuts at: 0 947 1020
Size: 947 73
NaeI GCC'GGC
Cuts at: 0 655 1020
Size: 655 365
NarI GG'CG_CC
Cuts at: 0 846 1020
Size: 846 174
NdeI CA'TA_TG
Cuts at: 0 312 1020
Size: 312 708
NgoMIV G'CCGG_C
Cuts at: 0 653 1020
Size: 653 367
NsiI A_TGCA'T
Cuts at: 0 720 1020
Size: 720 300
NspI r_CATG'y
Cuts at: 0 74 651 1020
Size: 74 577 369
Fragments arranged by size:
577 369 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 185 629 1020
Size: 185 444 391
Fragments arranged by size:
444 391 185
PfoI T'CCnGG_A
Cuts at: 0 452 1020
Size: 452 568
PmlI CAC'GTG
Cuts at: 0 494 1020
Size: 494 526
PpuMI rG'GwC_Cy
Cuts at: 0 989 1020
Size: 989 31
PstI C_TGCA'G
Cuts at: 0 751 1020
Size: 751 269
PvuII CAG'CTG
Cuts at: 0 947 1020
Size: 947 73
SacI G_AGCT'C
Cuts at: 0 782 1020
Size: 782 238
SbfI CC_TGCA'GG
Cuts at: 0 751 1020
Size: 751 269
SfcI C'TryA_G
Cuts at: 0 747 1020
Size: 747 273
SfoI GGC'GCC
Cuts at: 0 847 1020
Size: 847 173
SmlI C'TyrA_G
Cuts at: 0 40 1006 1020
Size: 40 966 14
Fragments arranged by size:
966 40 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 1020
Size: 57 32 931
Fragments arranged by size:
931 57 32
Tth111I GACn'n_nGTC
Cuts at: 0 987 1020
Size: 987 33
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI BanI BanII BbeI
BfrBI BglI BglII Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstEII BstYI BtsI DraIII DrdI
EaeI EcoICRI Eco57MI EcoO109I FspI FspAI HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PflMI PfoI PmlI PpuMI PstI PvuII
SacI SbfI SfcI SfoI SmlI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BlpI BmgBI BmtI Bpu10I BsiWI BsmI BspEI
BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI HincII HindIII HpaI KpnI
MfeI MluI MmeI NcoI NheI NotI NruI PacI
PasI PciI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI XbaI
XcmI XhoI XmaI XmnI ZraI