Restriction map for
pFN21AB3424
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
BbeI BbrPI BcgI BciVI BcnI
BglI BglII BlpI Bpu1102I BsaAI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsmBI BsoBI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI KspI MfeI MluI MroI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 1035 CCGC CCGC(-3/-1)
AcyI 862 GACGCC G[GA]^CG[CT]C
AflII 1218 CTTAAG C^TTAAG
ApaI 787 GGGCCC GGGCC^C
AvrII 777 CCTAGG C^CTAGG
BalI 986 TGGCCA TGG^CCA
BanI 1297 GGCACC G^G[CT][GA]CC
BbsI 1249 GAAGAC GAAGAC(2/6)
BbvCI 728 CCTCAGC CCTCAGC(-5/-2)
BclI 1148 TGATCA T^GATCA
BfrI 1218 CTTAAG C^TTAAG
BlnI 777 CCTAGG C^CTAGG
BpmI 32 CTGGAG CTGGAG(16/14)
BpuAI 1249 GAAGAC GAAGAC(2/6)
BsaBI 349 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 862 GACGCC G[GA]^CG[CT]C
BsaI 544 GAGACC GGTCTC(1/5)
BseRI 277 GAGGAG GAGGAG(10/8)
BsiWI 798 CGTACG C^GTACG
BsmAI 544 GAGAC GTCTC(1/5)
BsmI 401 GCATTC GAATGC(1/-1)
Bsp1407I 1195 TGTACA T^GTACA
BsrFI 173 GCCGGT [GA]^CCGG[CT]
BsrGI 1195 TGTACA T^GTACA
BstXI 942 CCAGTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1086 GCGC GCG^C
Cfr10I 173 GCCGGT [GA]^CCGG[CT]
DrdI 312 GACCCCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 986 TGGCCA [CT]^GGCC[GA]
EcoNI 682 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 229 GAATTC G^AATTC
FbaI 1148 TGATCA T^GATCA
HapII 174 CCGG C^CGG
HhaI 1086 GCGC GCG^C
Hin1I 862 GACGCC G[GA]^CG[CT]C
HinP1I 1086 GCGC G^CGC
HpaII 174 CCGG C^CGG
MamI 349 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 986 TGGCCA TGG^CCA
MscI 986 TGGCCA TGG^CCA
MslI 513 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 174 CCGG C^CGG
PleI 886 GACTC GAGTC(4/5)
PpuMI 310 GGGACCC [GA]G^G[AT]CC[CT]
SphI 468 GCATGC GCATG^C
SspBI 1195 TGTACA T^GTACA
SspI 626 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 369 GTCTAC GT^[AC][GT]AC
415 GTAGAC
AluI 605 AGCT AG^CT
1203 AGCT
AlwNI 128 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1199 CAGAAGCTG
AocI 655 CCTAAGG CC^T[ACGT]AGG
1303 CCTGAGG
Asp700I 760 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1270 GAAAATGTTC
BamHI 501 GGATCC G^GATCC
1011 GGATCC
BanII 381 GGGCTC G[GA]GC[CT]^C
787 GGGCCC
BbvI 133 GCTGC GCAGC(8/12)
440 GCTGC
BmyI 381 GGGCTC G[AGT]GC[ACT]^C
787 GGGCCC
Bsp1286I 381 GGGCTC G[AGT]GC[ACT]^C
787 GGGCCC
BsrI 150 ACTGG ACTGG(1/-1)
942 CCAGT
Bsu36I 655 CCTAAGG CC^T[ACGT]AGG
1303 CCTGAGG
DraII 310 GGGACCC [GA]G^G[ACGT]CC[CT]
533 AGGCCCC
DraIII 165 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
460 CACAAGGTG
EarI 337 GAAGAG CTCTTC(1/4)
634 GAAGAG
Eco81I 655 CCTAAGG CC^T[ACGT]AGG
1303 CCTGAGG
EcoO109I 310 GGGACCC [GA]G^G[ACGT]CC[CT]
533 AGGCCCC
EcoT14I 332 CCTTGG C^C[AT][AT]GG
777 CCTAGG
HgaI 862 GACGC GACGC(5/10)
1116 GACGC
Ksp632I 337 GAAGAG CTCTTC(1/4)
634 GAAGAG
MaeII 395 ACGT A^CGT
1227 ACGT
NspI 468 GCATGC [GA]CATG^[CT]
828 ACATGC
SfaNI 130 GATGC GCATC(5/9)
1088 GCATC
SmlI 741 CTTGAG C^T[CT][GA]AG
1218 CTTAAG
StyI 332 CCTTGG C^C[AT][AT]GG
777 CCTAGG
TseI 133 GCTGC G^C[AT]GC
440 GCTGC
Tsp45I 154 GTGAC ^GT[GC]AC
511 GTCAC
XmnI 760 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1270 GAAAATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AcsI AfaI AlwI ApoI AvaII
BfaI BsaJI BsiYI BslI BsmFI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TfiI Tru9I Tsp509I TspRI XhoII