(Circular) (Six-base) MAPSORT of: pFN21AB3424.seq Check: 3543 from: 1 to: 6025 pFN21AB3424 6025 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3835 278 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2375 2421 3368 1937 Size: 438 46 947 4594 Fragments arranged by size: 4594 947 438 46 Acc65I G'GTAC_C Cuts at: 3346 3346 Size: 6025 AclI AA'CG_TT Cuts at: 1563 4152 4525 1563 Size: 2589 373 3063 Fragments arranged by size: 3063 2589 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 2847 4088 5472 5740 1632 Size: 581 634 1241 1384 268 1917 Fragments arranged by size: 1917 1384 1241 634 581 268 AflII C'TTAA_G Cuts at: 820 1017 3223 820 Size: 197 2206 3622 Fragments arranged by size: 3622 2206 197 AflIII A'CryG_T Cuts at: 1203 4924 5901 1203 Size: 3721 977 1327 Fragments arranged by size: 3721 1327 977 AgeI A'CCGG_T Cuts at: 4856 4856 Size: 6025 AhdI GACnn_n'nnGTC Cuts at: 4754 4754 Size: 6025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6025 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6025 AlwNI CAG_nnn'CTG Cuts at: 2138 3209 3768 5340 2138 Size: 1071 559 1572 2823 Fragments arranged by size: 2823 1572 1071 559 ApaI G_GGCC'C Cuts at: 2796 2796 Size: 6025 ApaLI G'TGCA_C Cuts at: 4082 5238 4082 Size: 1156 4869 ApoI r'AATT_y Cuts at: 1493 2234 2583 2916 3334 3560 3712 5821 1493 Size: 741 349 333 418 226 152 2109 1697 Fragments arranged by size: 2109 1697 741 418 349 333 226 152 AseI AT'TA_AT Cuts at: 160 1032 4579 160 Size: 872 3547 1606 Fragments arranged by size: 3547 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 AvaI C'yCGr_G Cuts at: 1870 1943 3350 1870 Size: 73 1407 4545 Fragments arranged by size: 4545 1407 73 AvrII C'CTAG_G Cuts at: 2782 2782 Size: 6025 BamHI G'GATC_C Cuts at: 2506 3016 3355 3722 2506 Size: 510 339 367 4809 Fragments arranged by size: 4809 510 367 339 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3302 3346 4801 618 Size: 325 217 627 1515 44 1455 1842 Fragments arranged by size: 1842 1515 1455 627 325 217 44 BanII G_rGCy'C Cuts at: 1391 2390 2796 3344 1391 Size: 999 406 548 4072 Fragments arranged by size: 4072 999 548 406 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3261 928 Size: 971 1362 3692 Fragments arranged by size: 3692 1362 971 BbvCI CC'TCA_GC Cuts at: 2734 2734 Size: 6025 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4216 4250 1903 Size: 34 2279 34 3678 Fragments arranged by size: 3678 2279 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3917 5127 3917 Size: 1210 4815 BclI T'GATC_A Cuts at: 1548 3153 1548 Size: 1605 4420 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4636 136 Size: 107 122 71 631 605 2964 1525 Fragments arranged by size: 2964 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6020 6020 Size: 6025 BlpI GC'TnA_GC Cuts at: 3442 3442 Size: 6025 Bme1580I G_kGCm'C Cuts at: 1790 2796 4086 5242 1790 Size: 1006 1290 1156 2573 Fragments arranged by size: 2573 1290 1156 1006 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3756 4714 442 Size: 416 53 83 399 24 2339 958 1753 Fragments arranged by size: 2339 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6025 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 4685 1239 Size: 819 2627 2579 Fragments arranged by size: 2627 2579 819 Bpu10I CC'TnA_GC Cuts at: 2734 2734 Size: 6025 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2767 4150 5015 5313 2767 Size: 1383 865 298 3479 Fragments arranged by size: 3479 1383 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2543 4688 882 Size: 617 1044 2145 2219 Fragments arranged by size: 2219 2145 1044 617 BsaAI yAC'GTr Cuts at: 493 1204 3816 493 Size: 711 2612 2702 Fragments arranged by size: 2702 2612 711 BsaBI GATnn'nnATC Cuts at: 2358 6019 2358 Size: 3661 2364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2868 3832 4214 4849 Size: 53 83 186 936 1335 964 382 635 Cuts at: 4849 275 Size: 1451 Fragments arranged by size: 1451 1335 964 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4458 4856 5130 5277 5688 4458 Size: 398 274 147 411 4795 Fragments arranged by size: 4795 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5800 Fragments arranged by size: 5800 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 1186 Size: 1111 4914 BseYI C'CCAG_C Cuts at: 5228 5724 5228 Size: 496 5529 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5452 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3475 4236 5264 1540 Size: 275 186 1474 761 1028 2301 Fragments arranged by size: 2301 1474 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4086 4171 5242 4086 Size: 85 1071 4869 Fragments arranged by size: 4869 1071 85 BsiWI C'GTAC_G Cuts at: 2803 2803 Size: 6025 BsmI GAATG_Cn' Cuts at: 2406 3536 3629 2406 Size: 1130 93 4802 Fragments arranged by size: 4802 1130 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2390 2796 3344 4086 4171 5242 1391 Size: 399 600 406 548 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 548 406 399 85 BspEI T'CCGG_A Cuts at: 5688 5688 Size: 6025 BspHI T'CATG_A Cuts at: 3914 5644 3914 Size: 1730 4295 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3380 844 Size: 2536 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3912 1394 Size: 429 2089 3507 Fragments arranged by size: 3507 2089 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4520 4694 65 Size: 1183 3272 174 1396 Fragments arranged by size: 3272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2178 4669 4856 1700 Size: 110 368 2491 187 2869 Fragments arranged by size: 2869 2491 368 187 110 BsrGI T'GTAC_A Cuts at: 96 3200 96 Size: 3104 2921 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6025 BssSI C'ACGA_G Cuts at: 4085 5097 4085 Size: 1012 5013 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6025 BstBI TT'CG_AA Cuts at: 4829 4829 Size: 6025 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6025 BstXI CCAn_nnnn'nTGG Cuts at: 2954 2954 Size: 6025 BstYI r'GATC_y Cuts at: 1972 2428 2506 3016 3355 3722 4107 4124 5576 Size: 456 78 510 339 367 385 17 1452 Cuts at: 5576 5662 5674 6020 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 510 456 385 367 346 339 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2661 3309 2661 Size: 648 5377 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5286 Fragments arranged by size: 5286 683 56 BtsI GCAGTG_nn' Cuts at: 3541 4335 4355 3541 Size: 794 20 5211 Fragments arranged by size: 5211 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6025 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2082 2117 2884 2919 529 Size: 35 1518 35 767 35 3635 Fragments arranged by size: 3635 1518 767 35 35 35 DraI TTT'AAA Cuts at: 3329 3329 Size: 6025 DraIII CAC_nnn'GTG Cuts at: 2175 2470 2175 Size: 295 5730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2323 5032 809 Size: 507 1007 2709 1802 Fragments arranged by size: 2709 1802 1007 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2991 3472 4361 Size: 54 1425 50 275 18 1161 481 889 Cuts at: 4361 5990 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1161 889 481 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3472 1537 Size: 275 1660 4090 Fragments arranged by size: 4090 1660 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2337 2634 3955 1353 Size: 984 297 1321 3423 Fragments arranged by size: 3423 1321 984 297 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4612 4986 5132 387 Size: 1064 138 3023 374 146 1280 Fragments arranged by size: 3023 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2213 2847 4088 4685 5472 5740 Size: 393 426 155 634 1241 597 787 268 Cuts at: 5740 1239 Size: 1524 Fragments arranged by size: 1524 1241 787 634 597 426 393 268 155 EcoNI CCTnn'n_nnAGG Cuts at: 2691 2691 Size: 6025 EcoO109I rG'GnC_Cy Cuts at: 2316 2539 2316 Size: 223 5802 EcoRI G'AATT_C Cuts at: 2234 3334 3712 2234 Size: 1100 378 4547 Fragments arranged by size: 4547 1100 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2234 3112 2234 Size: 878 5147 FspI TGC'GCA Cuts at: 4531 4531 Size: 6025 HaeII r_GCGC'y Cuts at: 1678 5172 1678 Size: 3494 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2011 2043 2276 2308 3158 Size: 32 135 32 439 32 233 32 850 Cuts at: 3158 3190 4666 4698 4740 4772 1373 Size: 32 1476 32 42 32 2626 Fragments arranged by size: 2626 1476 850 439 233 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3369 3615 669 Size: 988 281 1431 246 3079 Fragments arranged by size: 3079 1431 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6025 HpaI GTT'AAC Cuts at: 3615 3615 Size: 6025 KpnI G_GTAC'C Cuts at: 3350 3350 Size: 6025 MfeI C'AATT_G Cuts at: 3624 3624 Size: 6025 MluI A'CGCG_T Cuts at: 5901 5901 Size: 6025 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 2913 2931 5139 5323 1305 Size: 1248 360 18 2208 184 2007 Fragments arranged by size: 2208 2007 1248 360 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 2993 10 Size: 54 1425 343 1161 3042 Fragments arranged by size: 3042 1425 1161 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2522 3983 4342 4501 518 Size: 648 1356 1461 359 159 2042 Fragments arranged by size: 2042 1461 1356 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3441 4118 5266 5511 1606 Size: 1835 677 1148 245 2120 Fragments arranged by size: 2120 1835 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4534 Fragments arranged by size: 4534 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6025 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6025 NotI GC'GGCC_GC Cuts at: 3472 3472 Size: 6025 NspI r_CATG'y Cuts at: 1132 1229 2477 2837 3383 4928 1132 Size: 97 1248 360 546 1545 2229 Fragments arranged by size: 2229 1545 1248 546 360 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6025 PciI A'CATG_T Cuts at: 4924 4924 Size: 6025 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6025 PfoI T'CCnGG_A Cuts at: 2035 2035 Size: 6025 PmeI GTTT'AAAC Cuts at: 3329 3329 Size: 6025 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 5607 5639 4094 Size: 32 1481 32 4480 Fragments arranged by size: 4480 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2316 2316 Size: 6025 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6025 PsiI TTA'TAA Cuts at: 3288 3595 3288 Size: 307 5718 PspOMI G'GGCC_C Cuts at: 2792 2792 Size: 6025 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2073 2105 2073 Size: 32 5993 PstI C_TGCA'G Cuts at: 830 3377 830 Size: 2547 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6025 SalI G'TCGA_C Cuts at: 1936 3367 1936 Size: 1431 4594 SanDI GG'GwC_CC Cuts at: 2316 2316 Size: 6025 SbfI CC_TGCA'GG Cuts at: 3377 3377 Size: 6025 ScaI AGT'ACT Cuts at: 4273 5985 4273 Size: 1712 4313 SfcI C'TryA_G Cuts at: 826 1045 3373 4508 5189 5380 826 Size: 219 2328 1135 681 191 1471 Fragments arranged by size: 2328 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6025 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6025 SmaI CCC'GGG Cuts at: 1872 3352 1872 Size: 1480 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2746 3223 4129 5030 5292 820 Size: 197 926 803 477 906 901 262 1553 Fragments arranged by size: 1553 926 906 901 803 477 262 197 SnaBI TAC'GTA Cuts at: 493 3816 493 Size: 3323 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6025 SphI G_CATG'C Cuts at: 1132 2477 3383 1132 Size: 1345 906 3774 Fragments arranged by size: 3774 1345 906 SspI AAT'ATT Cuts at: 5 52 2633 3949 5 Size: 47 2581 1316 2081 Fragments arranged by size: 2581 2081 1316 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6025 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2782 3464 513 Size: 552 939 333 445 682 3074 Fragments arranged by size: 3074 939 682 552 445 333 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 2636 4052 4069 4222 4407 4852 Size: 1255 860 196 1416 17 153 185 445 Cuts at: 4852 325 Size: 1498 Fragments arranged by size: 1498 1416 1255 860 445 196 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3200 4271 5983 Size: 274 80 33 51 1443 1223 1071 1712 Cuts at: 5983 96 Size: 138 Fragments arranged by size: 1712 1443 1223 1071 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5985 6017 5985 Size: 32 5993 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6025 XbaI T'CTAG_A Cuts at: 3361 3361 Size: 6025 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6025 XmaI C'CCGG_G Cuts at: 1870 3350 1870 Size: 1480 4545 XmnI GAAnn'nnTTC Cuts at: 2769 3279 4154 2769 Size: 510 875 4640 Fragments arranged by size: 4640 875 510 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3833 276 Size: 53 83 186 936 2299 2468 Fragments arranged by size: 2468 2299 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BplI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI