Restriction map for pFN21AB3336
 (Linear) (Six-base) MAPSORT of: KIBB3336.seq  Check: 3120  from: 1  to: 1653



KIBB3336.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AatII G_ACGT'C

Cuts at:      0     899    1653 
   Size:        899     754
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     669     841     853     921    1653 
   Size:        669     172      12      68     732

  Fragments arranged by size: 

                 732     669     172      68      12
 
AflII C'TTAA_G

Cuts at:      0    1044    1653 
   Size:       1044     609
 
AlwNI CAG_nnn'CTG

Cuts at:      0     291     678    1230    1397    1413    1653 
   Size:        291     387     552     167      16     240

  Fragments arranged by size: 

                 552     387     291     240     167      16
 
ApaI G_GGCC'C

Cuts at:      0    1572    1653 
   Size:       1572      81
 
AvaI C'yCGr_G

Cuts at:      0     117    1653 
   Size:        117    1536
 
BanI G'GyrC_C

Cuts at:      0     954    1026    1194    1653 
   Size:        954      72     168     459

  Fragments arranged by size: 

                 954     459     168      72
 
BanII G_rGCy'C

Cuts at:      0     159     276     664     880    1037    1572    1653 
   Size:        159     117     388     216     157     535      81

  Fragments arranged by size: 

                 535     388     216     159     157     117      81
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1175    1199    1653 
   Size:       1175      24     454

  Fragments arranged by size: 

                1175     454      24
 
BbvCI CC'TCA_GC

Cuts at:      0     751    1653 
   Size:        751     902
 
BclI T'GATC_A

Cuts at:      0     791    1653 
   Size:        791     862
 
BglI GCCn_nnn'nGGC

Cuts at:      0     947    1416    1551    1653 
   Size:        947     469     135     102

  Fragments arranged by size: 

                 947     469     135     102
 
BglII A'GATC_T

Cuts at:      0     962    1653 
   Size:        962     691
 
BlpI GC'TnA_GC

Cuts at:      0     874    1653 
   Size:        874     779
 
Bme1580I G_kGCm'C

Cuts at:      0    1197    1572    1653 
   Size:       1197     375      81

  Fragments arranged by size: 

                1197     375      81
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      90     600    1289    1407    1653 
   Size:         90     510     689     118     246

  Fragments arranged by size: 

                 689     510     246     118      90
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     514     604     678     687    1003    1251    1609 
   Size:        162     352      90      74       9     316     248     358

Cuts at:   1609    1653 
   Size:         44

  Fragments arranged by size: 

                 358     352     316     248     162      90      74      44
                   9
 
Bpu10I CC'TnA_GC

Cuts at:      0     751    1031    1466    1653 
   Size:        751     280     435     187

  Fragments arranged by size: 

                 751     435     280     187
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541    1653 
   Size:        541    1112
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     424    1653 
   Size:        424    1229
 
BsaAI yAC'GTr

Cuts at:      0     426    1653 
   Size:        426    1227
 
BsaBI GATnn'nnATC

Cuts at:      0     358    1653 
   Size:        358    1295
 
BsaHI Gr'CG_yC

Cuts at:      0      24     896    1247    1653 
   Size:         24     872     351     406

  Fragments arranged by size: 

                 872     406     351      24
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     881     911    1653 
   Size:        881      30     742

  Fragments arranged by size: 

                 881     742      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25      34     550     730     905    1291    1507    1653 
   Size:         25       9     516     180     175     386     216     146

  Fragments arranged by size: 

                 516     386     216     180     175     146      25       9
            
 
BseYI C'CCAG_C

Cuts at:      0     452     869    1312    1406    1486    1653 
   Size:        452     417     443      94      80     167

  Fragments arranged by size: 

                 452     443     417     167      94      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1239    1653 
   Size:       1239     414
 
BsiHKAI G_wGCw'C

Cuts at:      0     880    1653 
   Size:        880     773
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     580     887    1653 
   Size:        580     307     766

  Fragments arranged by size: 

                 766     580     307
 
Bsp1286I G_dGCh'C

Cuts at:      0     159     276     664     880    1037    1197    1572    1653 
   Size:        159     117     388     216     157     160     375      81

  Fragments arranged by size: 

                 388     375     216     160     159     157     117      81
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1286    1653 
   Size:       1286     367
 
BsrFI r'CCGG_y

Cuts at:      0    1348    1653 
   Size:       1348     305
 
BssSI C'ACGA_G

Cuts at:      0     742    1653 
   Size:        742     911
 
BstYI r'GATC_y

Cuts at:      0     575     962    1653 
   Size:        575     387     691

  Fragments arranged by size: 

                 691     575     387
 
Bsu36I CC'TnA_GG

Cuts at:      0     316    1653 
   Size:        316    1337
 
BtgI C'CryG_G

Cuts at:      0     724     814    1653 
   Size:        724      90     839

  Fragments arranged by size: 

                 839     724      90
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     357    1653 
   Size:        357    1296
 
BtsI GCAGTG_nn'

Cuts at:      0    1325    1347    1653 
   Size:       1325      22     306

  Fragments arranged by size: 

                1325     306      22
 
DraIII CAC_nnn'GTG

Cuts at:      0    1650    1653 
   Size:       1650       3
 
EaeI y'GGCC_r

Cuts at:      0     190     356     732    1187    1653 
   Size:        190     166     376     455     466

  Fragments arranged by size: 

                 466     455     376     190     166
 
EarI CTCTTCn'nnn_

Cuts at:      0     918    1291    1349    1653 
   Size:        918     373      58     304

  Fragments arranged by size: 

                 918     373     304      58
 
EcoICRI GAG'CTC

Cuts at:      0     878    1653 
   Size:        878     775
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     514     604     669     678     687     841     853 
   Size:        162     352      90      65       9       9     154      12

Cuts at:    853     921    1003    1251    1609    1653 
   Size:         68      82     248     358      44

  Fragments arranged by size: 

                 358     352     248     162     154      90      82      68
                  65      44      12       9       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     638     709    1015    1232    1539    1653 
   Size:        638      71     306     217     307     114

  Fragments arranged by size: 

                 638     307     306     217     114      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     540     828    1401    1417    1497    1568    1569    1635 
   Size:        540     288     573      16      80      71       1      66

Cuts at:   1635    1653 
   Size:         18

  Fragments arranged by size: 

                 573     540     288      80      71      66      18      16
                   1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     113     145     881     913    1024    1056    1144    1176 
   Size:        113      32     736      32     111      32      88      32

Cuts at:   1176    1653 
   Size:        477

  Fragments arranged by size: 

                 736     477     113     111      88      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     303    1653 
   Size:        303    1350
 
MscI TGG'CCA

Cuts at:      0     358    1653 
   Size:        358    1295
 
MslI CAynn'nnrTG

Cuts at:      0     778    1653 
   Size:        778     875
 
MspA1I CmG'CkG

Cuts at:      0      79     755     873    1005    1070    1653 
   Size:         79     676     118     132      65     583

  Fragments arranged by size: 

                 676     583     132     118      79      65
 
NcoI C'CATG_G

Cuts at:      0     814    1653 
   Size:        814     839
 
NspI r_CATG'y

Cuts at:      0     107    1653 
   Size:        107    1546
 
PasI CC'CwG_GG

Cuts at:      0    1333    1413    1514    1653 
   Size:       1333      80     101     139

  Fragments arranged by size: 

                1333     139     101      80
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     678     725    1397    1413    1653 
   Size:        678      47     672      16     240

  Fragments arranged by size: 

                 678     672     240      47      16
 
PfoI T'CCnGG_A

Cuts at:      0     362    1106    1653 
   Size:        362     744     547

  Fragments arranged by size: 

                 744     547     362
 
PmlI CAC'GTG

Cuts at:      0     426    1653 
   Size:        426    1227
 
PpuMI rG'GwC_Cy

Cuts at:      0     828    1401    1497    1635    1653 
   Size:        828     573      96     138      18

  Fragments arranged by size: 

                 828     573     138      96      18
 
PspOMI G'GGCC_C

Cuts at:      0    1568    1653 
   Size:       1568      85
 
PstI C_TGCA'G

Cuts at:      0      78     164     702     802    1016    1226    1653 
   Size:         78      86     538     100     214     210     427

  Fragments arranged by size: 

                 538     427     214     210     100      86      78
 
PvuII CAG'CTG

Cuts at:      0     873    1005    1070    1653 
   Size:        873     132      65     583

  Fragments arranged by size: 

                 873     583     132      65
 
SacI G_AGCT'C

Cuts at:      0     880    1653 
   Size:        880     773
 
SanDI GG'GwC_CC

Cuts at:      0    1401    1653 
   Size:       1401     252
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1291    1653 
   Size:       1291     362
 
SexAI A'CCwGG_T

Cuts at:      0    1442    1653 
   Size:       1442     211
 
SfcI C'TryA_G

Cuts at:      0      74     160     698     798    1012    1222    1653 
   Size:         74      86     538     100     214     210     431

  Fragments arranged by size: 

                 538     431     214     210     100      86      74
 
SmlI C'TyrA_G

Cuts at:      0     117     556    1044    1653 
   Size:        117     439     488     609

  Fragments arranged by size: 

                 609     488     439     117
 
StuI AGG'CCT

Cuts at:      0    1129    1545    1653 
   Size:       1129     416     108

  Fragments arranged by size: 

                1129     416     108
 
StyI C'CwwG_G

Cuts at:      0     705     814    1115    1653 
   Size:        705     109     301     538

  Fragments arranged by size: 

                 705     538     301     109
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     619    1212    1653 
   Size:        619     593     441

  Fragments arranged by size: 

                 619     593     441
 
TatI w'GTAC_w

Cuts at:      0     782    1139    1653 
   Size:        782     357     514

  Fragments arranged by size: 

                 782     514     357
 
Tth111I GACn'n_nGTC

Cuts at:      0     307     513    1653 
   Size:        307     206    1140

  Fragments arranged by size: 

                1140     307     206
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     527    1653 
   Size:        527    1126
 
XhoI C'TCGA_G

Cuts at:      0     117    1653 
   Size:        117    1536
 
ZraI GAC'GTC

Cuts at:      0     897    1653 
   Size:        897     756
 

 Enzymes that do cut:

    AatII     AcuI    AflII    AlwNI     ApaI     AvaI     BanI    BanII
     BbsI    BbvCI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrFI
    BssSI    BstYI   Bsu36I     BtgI    BtgZI     BtsI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII     MscI
     MslI   MspA1I     NcoI     NspI     PasI    PflMI     PfoI     PmlI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     SapI    SexAI
     SfcI     SmlI     StuI     StyI    TaqII     TatI  Tth111I     XcmI
     XhoI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI    BmgBI
     BmtI     BplI    BsaWI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TstI     XbaI
     XmaI     XmnI
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