Restriction map for pFN21AB3095
 (Circular) (Six-base) MAPSORT of: pFN21AB3095.seq  Check: 2545  from: 1  to: 5686



pFN21AB3095  5686 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:24  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2944    3496     278 
   Size:         53      83     186     936    1408     552    2468

  Fragments arranged by size: 

                2468    1408     936     552     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2207    3029    1937 
   Size:        270     822    4594

  Fragments arranged by size: 

                4594     822     270
 
Acc65I G'GTAC_C

Cuts at:   3007    3007 
   Size:       5686
 
AclI AA'CG_TT

Cuts at:   1563    3813    4186    1563 
   Size:       2250     373    3063

  Fragments arranged by size: 

                3063    2250     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2115    3749    5133    5401    1632 
   Size:        483    1634    1384     268    1917

  Fragments arranged by size: 

                1917    1634    1384     483     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2362     820 
   Size:        197    1345    4144

  Fragments arranged by size: 

                4144    1345     197
 
AflIII A'CryG_T

Cuts at:   1203    4585    5562    1203 
   Size:       3382     977    1327

  Fragments arranged by size: 

                3382    1327     977
 
AgeI A'CCGG_T

Cuts at:   4517    4517 
   Size:       5686
 
AhdI GACnn_n'nnGTC

Cuts at:   2945    4415    2945 
   Size:       1470    4216
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5686
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5686
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2953    2985    2953 
   Size:         32    5654
 
AlwNI CAG_nnn'CTG

Cuts at:   3429    5001    3429 
   Size:       1572    4114
 
ApaLI G'TGCA_C

Cuts at:   3743    4899    3743 
   Size:       1156    4530
 
ApoI r'AATT_y

Cuts at:   1493    2181    2702    2768    2932    2995    3221    3373    5482 
   Size:        688     521      66     164      63     226     152    2109

Cuts at:   5482    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     688     521     226     164     152      66
                  63
 
AseI AT'TA_AT

Cuts at:    160    1032    4240     160 
   Size:        872    3208    1606

  Fragments arranged by size: 

                3208    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5686
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2127    3011    1870 
   Size:         73     184     884    4545

  Fragments arranged by size: 

                4545     884     184      73
 
BamHI G'GATC_C

Cuts at:   3016    3383    3016 
   Size:        367    5319
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3007    4462     618 
   Size:        325     217     627    1220    1455    1842

  Fragments arranged by size: 

                1842    1455    1220     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2743    3005    1391 
   Size:       1352     262    4072

  Fragments arranged by size: 

                4072    1352     262
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2456     928 
   Size:        971     557    4158

  Fragments arranged by size: 

                4158     971     557
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3877    3911    1903 
   Size:         34    1940      34    3678

  Fragments arranged by size: 

                3678    1940      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3578    4788    3578 
   Size:       1210    4476
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5686
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4297     136 
   Size:        107     122      71     631     605    2625    1525

  Fragments arranged by size: 

                2625    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2133    2505    5681    2133 
   Size:        372    3176    2138

  Fragments arranged by size: 

                3176    2138     372
 
BlpI GC'TnA_GC

Cuts at:   3103    3103 
   Size:       5686
 
Bme1580I G_kGCm'C

Cuts at:   1790    3747    4903    1790 
   Size:       1957    1156    2573

  Fragments arranged by size: 

                2573    1957    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5686
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3417    4375     442 
   Size:        416      53      83     399      24    2000     958    1753

  Fragments arranged by size: 

                2000    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2388    1056 
   Size:       1332    4354
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2072    4346    1239 
   Size:        833    2274    2579

  Fragments arranged by size: 

                2579    2274     833
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3811    4676    4974    3811 
   Size:        865     298    4523

  Fragments arranged by size: 

                4523     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2566    4349     882 
   Size:        617    1067    1783    2219

  Fragments arranged by size: 

                2219    1783    1067     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3477     493 
   Size:        711    2273    2702

  Fragments arranged by size: 

                2702    2273     711
 
BsaBI GATnn'nnATC

Cuts at:   5680    5680 
   Size:       5686
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2941    3493    3875    4510 
   Size:         53      83     186     936    1408     552     382     635

Cuts at:   4510     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1408     936     635     552     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4119    4517    4791    4938    5349    4119 
   Size:        398     274     147     411    4456

  Fragments arranged by size: 

                4456     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2265    2295    2676    2706    1178 
   Size:         30     165      30     862      30     381      30    4158

  Fragments arranged by size: 

                4158     862     381     165      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5686
 
BseYI C'CCAG_C

Cuts at:   2646    4889    5385    2646 
   Size:       2243     496    2947

  Fragments arranged by size: 

                2947    2243     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2105    1163 
   Size:        573     369    4744

  Fragments arranged by size: 

                4744     573     369
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3136    3897    4925    1540 
   Size:        275     186    1135     761    1028    2301

  Fragments arranged by size: 

                2301    1135    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2743    3747    3832    4903    2743 
   Size:       1004      85    1071    3526

  Fragments arranged by size: 

                3526    1071    1004      85
 
BsmI GAATG_Cn'

Cuts at:   2423    3197    3290    2423 
   Size:        774      93    4819

  Fragments arranged by size: 

                4819     774      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2743    3005    3747    3832    4903    1391 
   Size:        399     953     262     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     953     742     399     262      85
 
BspEI T'CCGG_A

Cuts at:   5349    5349 
   Size:       5686
 
BspHI T'CATG_A

Cuts at:   2716    3575    5305    2716 
   Size:        859    1730    3097

  Fragments arranged by size: 

                3097    1730     859
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2547    3041     844 
   Size:       1703     494    3489

  Fragments arranged by size: 

                3489    1703     494
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3573    1394 
   Size:        429    1750    3507

  Fragments arranged by size: 

                3507    1750     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4181    4355      65 
   Size:       1183    2933     174    1396

  Fragments arranged by size: 

                2933    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4330    4517    1700 
   Size:        110    2520     187    2869

  Fragments arranged by size: 

                2869    2520     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5686
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5686
 
BssSI C'ACGA_G

Cuts at:   3746    4758    3746 
   Size:       1012    4674
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5686
 
BstBI TT'CG_AA

Cuts at:   4490    4490 
   Size:       5686
 
BstEII G'GTnAC_C

Cuts at:   1184    2791    1184 
   Size:       1607    4079
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2924    2924 
   Size:       5686
 
BstYI r'GATC_y

Cuts at:   1972    2133    2505    3016    3383    3768    3785    5237    5323 
   Size:        161     372     511     367     385      17    1452      86

Cuts at:   5323    5335    5681    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     511     385     372     367     346     161
                  86      17      12
 
BstZ17I GTA'TAC

Cuts at:   2208    2208 
   Size:       5686
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4195

  Fragments arranged by size: 

                4195     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4947

  Fragments arranged by size: 

                4947     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3202    3996    4016    3202 
   Size:        794      20    4872

  Fragments arranged by size: 

                4872     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5686
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5651
 
DraI TTT'AAA

Cuts at:   2990    2990 
   Size:       5686
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4693     809 
   Size:        507    3377    1802

  Fragments arranged by size: 

                3377    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    3133    4022    5651 
   Size:         54    1425      50     275      18    1303     889    1629

Cuts at:   5651       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1303     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3133    1537 
   Size:        275    1321    4090

  Fragments arranged by size: 

                4090    1321     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3616    1353 
   Size:       2263    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2024    2961    4273    4647    4793     387 
   Size:       1064     138     435     937    1312     374     146    1280

  Fragments arranged by size: 

                1312    1280    1064     937     435     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   2741    2741 
   Size:       5686
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2072    2115    3749    4346    5133    5401    1239 
   Size:        393     440      43    1634     597     787     268    1524

  Fragments arranged by size: 

                1634    1524     787     597     440     393     268      43
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2592    2592 
   Size:       5686
 
EcoRI G'AATT_C

Cuts at:   2932    2995    3373    2932 
   Size:         63     378    5245

  Fragments arranged by size: 

                5245     378      63
 
FspI TGC'GCA

Cuts at:   4192    4192 
   Size:       5686
 
HaeII r_GCGC'y

Cuts at:   1678    4833    1678 
   Size:       3155    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2528    2560    2931    2963    4327 
   Size:         32     135      32     956      32     371      32    1364

Cuts at:   4327    4359    4401    4433    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1364     956     371     135      42      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3030    3276     669 
   Size:        988     281    1092     246    3079

  Fragments arranged by size: 

                3079    1092     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5686
 
HpaI GTT'AAC

Cuts at:   3276    3276 
   Size:       5686
 
KpnI G_GTAC'C

Cuts at:   3011    3011 
   Size:       5686
 
MfeI C'AATT_G

Cuts at:   3285    3285 
   Size:       5686
 
MluI A'CGCG_T

Cuts at:   5562    5562 
   Size:       5686
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4800    4984    1305 
   Size:       3495     184    2007

  Fragments arranged by size: 

                3495    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3864

  Fragments arranged by size: 

                3864    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2379    3644    4003    4162     518 
   Size:        648    1213    1265     359     159    2042

  Fragments arranged by size: 

                2042    1265    1213     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2176    3102    3779    4927    5172    1606 
   Size:        570     926     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     926     677     570     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4195

  Fragments arranged by size: 

                4195     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5686
 
NheI G'CTAG_C

Cuts at:   1052    2384    1052 
   Size:       1332    4354
 
NotI GC'GGCC_GC

Cuts at:   3133    3133 
   Size:       5686
 
NspI r_CATG'y

Cuts at:   1132    1229    3044    4589    1132 
   Size:         97    1815    1545    2229

  Fragments arranged by size: 

                2229    1815    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5686
 
PciI A'CATG_T

Cuts at:   4585    4585 
   Size:       5686
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5686
 
PfoI T'CCnGG_A

Cuts at:   2071    2071 
   Size:       5686
 
PmeI GTTT'AAAC

Cuts at:   2990    2990 
   Size:       5686
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3755    3787    5268    5300    3755 
   Size:         32    1481      32    4141

  Fragments arranged by size: 

                4141    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5686
 
PsiI TTA'TAA

Cuts at:   2331    3256    2331 
   Size:        925    4761
 
PstI C_TGCA'G

Cuts at:    830    3038     830 
   Size:       2208    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5686
 
SacI G_AGCT'C

Cuts at:   2743    2743 
   Size:       5686
 
SalI G'TCGA_C

Cuts at:   1936    3028    1936 
   Size:       1092    4594
 
SbfI CC_TGCA'GG

Cuts at:   3038    3038 
   Size:       5686
 
ScaI AGT'ACT

Cuts at:   3934    5646    3934 
   Size:       1712    3974
 
SfcI C'TryA_G

Cuts at:    826    1045    2481    3034    4169    4850    5041     826 
   Size:        219    1436     553    1135     681     191    1471

  Fragments arranged by size: 

                1471    1436    1135     681     553     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5686
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5686
 
SmaI CCC'GGG

Cuts at:   1872    3013    1872 
   Size:       1141    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2362    3790    4691    4953     820 
   Size:        197     926     419    1428     901     262    1553

  Fragments arranged by size: 

                1553    1428     926     901     419     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3477     493 
   Size:       2984    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5686
 
SphI G_CATG'C

Cuts at:   1132    3044    1132 
   Size:       1912    3774
 
SspI AAT'ATT

Cuts at:      5      52    2437    3610       5 
   Size:         47    2385    1173    2081

  Fragments arranged by size: 

                2385    2081    1173      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5686
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3125     513 
   Size:        552     939    1121    3074

  Fragments arranged by size: 

                3074    1121     939     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3713    3730    3883    4068    4513     325 
   Size:       1255    2133      17     153     185     445    1498

  Fragments arranged by size: 

                2133    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3932    5644      96 
   Size:        274      80      33      51    1443    1955    1712     138

  Fragments arranged by size: 

                1955    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2067    2099    2265    2297    5646    5678    2067 
   Size:         32     166      32    3349      32    2075

  Fragments arranged by size: 

                3349    2075     166      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5686
 
XbaI T'CTAG_A

Cuts at:   3022    3022 
   Size:       5686
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5686
 
XmaI C'CCGG_G

Cuts at:   1870    3011    1870 
   Size:       1141    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2453    2507    3815    2453 
   Size:         54    1308    4324

  Fragments arranged by size: 

                4324    1308      54
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2942    3494     276 
   Size:         53      83     186     936    1408     552    2468

  Fragments arranged by size: 

                2468    1408     936     552     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SacI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI   Bsu36I   DraIII EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII    SacII    SanDI     SapI    SexAI     SfiI     SfoI
     SrfI     SwaI     XcmI
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