Restriction map for
pFN21AA1408
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Aor51HI AscI Asp718I AspI
AvaI AviII AvrII BanI BbeI
BbrPI BcgI BciVI BcnI BglI
BlnI BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BsoBI BspDI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstZ17I Cfr10I
ClaI CpoI DrdI DsaI EagI
EclXI Eco47III Eco52I EcoNI FauI
FseI FspI HgaI Hin1I KasI
KpnI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacII
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I SwaI Tth111I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 935 GTCGAC GT^[AC][GT]AC
AflII 3304 CTTAAG C^TTAAG
AhdI 559 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2163 CCTGAGG CC^T[ACGT]AGG
ApaI 3457 GGGCCC GGGCC^C
AspEI 559 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2787 TGGCCA TGG^CCA
BamHI 2525 GGATCC G^GATCC
BclI 2924 TGATCA T^GATCA
BfrI 3304 CTTAAG C^TTAAG
BlpI 7 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 7 GCTGAGC GC^T[ACGT]AGC
BsaBI 2439 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 3810 GAGACG CGTCTC(1/5)
Bsp1407I 2336 TGTACA T^GTACA
BspMI 2994 GCAGGT ACCTGC(4/8)
BsrGI 2336 TGTACA T^GTACA
BstEII 2355 GGTTACC G^GT[ACGT]ACC
BstPI 2355 GGTTACC G^GT[ACGT]ACC
Bsu36I 2163 CCTGAGG CC^T[ACGT]AGG
CelII 7 GCTGAGC GC^T[ACGT]AGC
DraIII 2530 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2787 TGGCCA [CT]^GGCC[GA]
Eam1105I 559 GACTCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2163 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2355 GGTTACC G^GT[ACGT]ACC
EcoRI 3016 GAATTC G^AATTC
EcoT14I 970 CCAAGG C^C[AT][AT]GG
FbaI 2924 TGATCA T^GATCA
HaeII 3710 AGCGCC [GA]GCGC^[CT]
HindIII 398 AAGCTT A^AGCTT
HpaI 3519 GTTAAC GTT^AAC
MamI 2439 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2787 TGGCCA TGG^CCA
MscI 2787 TGGCCA TGG^CCA
PflMI 2529 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 935 GTCGAC G^TCGAC
SapI 316 GAAGAGC GCTCTTC(1/4)
SexAI 1335 ACCTGGT A^CC[AT]GGT
SpeI 2499 ACTAGT A^CTAGT
SphI 2107 GCATGC GCATG^C
SspBI 2336 TGTACA T^GTACA
StuI 3033 AGGCCT AGG^CCT
StyI 970 CCAAGG C^C[AT][AT]GG
Tsp45I 2233 GTCAC ^GT[GC]AC
Van91I 2529 CCACGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1572 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccIII 453 TCCGGA T^CCGGA
2324 TCCGGA
AflIII 2125 ACATGT A^C[GA][CT]GT
2782 ACATGT
Alw44I 667 GTGCAC G^TGCAC
3403 GTGCAC
AlwNI 608 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1219 CAGAAGCTG
ApaLI 667 GTGCAC G^TGCAC
3403 GTGCAC
AseI 883 ATTAAT AT^TAAT
1579 ATTAAT
AsnI 883 ATTAAT AT^TAAT
1579 ATTAAT
Asp700I 376 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2551 GAAGATATTC
BbsI 160 GAAGAC GAAGAC(2/6)
202 GAAGAC
BbvCI 1929 GCTGAGG CCTCAGC(-5/-2)
3447 GCTGAGG
BpuAI 160 GAAGAC GAAGAC(2/6)
202 GAAGAC
BsaWI 453 TCCGGA [AT]^CCGG[AT]
2324 TCCGGA
BseAI 453 TCCGGA T^CCGGA
2324 TCCGGA
BsmI 2247 GAATGC GAATGC(1/-1)
1148 GCATTC
BspEI 453 TCCGGA T^CCGGA
2324 TCCGGA
BspLU11I 2125 ACATGT A^CATGT
2782 ACATGT
BstAPI 2205 GCAAGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3772 GCACCTAATGC
EcoRV 118 GATATC GAT^ATC
3652 GATATC
EcoT22I 2212 ATGCAT ATGCA^T
2249 ATGCAT
HapII 454 CCGG C^CGG
2325 CCGG
HpaII 454 CCGG C^CGG
2325 CCGG
MroI 453 TCCGGA T^CCGGA
2324 TCCGGA
MspI 454 CCGG C^CGG
2325 CCGG
NsiI 2212 ATGCAT ATGCA^T
2249 ATGCAT
PshBI 883 ATTAAT AT^TAAT
1579 ATTAAT
SacI 1117 GAGCTC GAGCT^C
1168 GAGCTC
ScaI 1073 AGTACT AGT^ACT
2792 AGTACT
SspI 877 AATATT AAT^ATT
1975 AATATT
XmnI 376 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2551 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BglII BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII