(Circular) (Six-base) MAPSORT of: pFN21AA1408.seq Check: 3293 from: 1 to: 8521 pFN21AA1408 8521 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6331 278 Size: 53 83 186 936 4795 2468 Fragments arranged by size: 4795 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2941 5864 1937 Size: 1004 2923 4594 Fragments arranged by size: 4594 2923 1004 Acc65I G'GTAC_C Cuts at: 5842 5842 Size: 8521 AclI AA'CG_TT Cuts at: 1563 6648 7021 1563 Size: 5085 373 3063 Fragments arranged by size: 5085 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2184 2470 2711 3645 6584 7968 8236 1632 Size: 552 286 241 934 2939 1384 268 1917 Fragments arranged by size: 2939 1917 1384 934 552 286 268 241 AflII C'TTAA_G Cuts at: 820 1017 5309 820 Size: 197 4292 4032 Fragments arranged by size: 4292 4032 197 AflIII A'CryG_T Cuts at: 1203 4130 4787 5818 7420 8397 1203 Size: 2927 657 1031 1602 977 1327 Fragments arranged by size: 2927 1602 1327 1031 977 657 AgeI A'CCGG_T Cuts at: 7352 7352 Size: 8521 AhdI GACnn_n'nnGTC Cuts at: 2569 7250 2569 Size: 4681 3840 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4242 5724 1184 Size: 3058 1482 3981 Fragments arranged by size: 3981 3058 1482 AlwNI CAG_nnn'CTG Cuts at: 2618 3229 6264 7836 2618 Size: 611 3035 1572 3303 Fragments arranged by size: 3303 3035 1572 611 ApaI G_GGCC'C Cuts at: 5466 5466 Size: 8521 ApaLI G'TGCA_C Cuts at: 2672 5408 6578 7734 2672 Size: 2736 1170 1156 3459 Fragments arranged by size: 3459 2736 1170 1156 ApoI r'AATT_y Cuts at: 1493 2593 3206 3238 3400 4592 5021 5830 6056 Size: 1100 613 32 162 1192 429 809 226 Cuts at: 6056 6208 8317 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1192 1100 809 613 429 226 162 152 32 AseI AT'TA_AT Cuts at: 160 1032 2889 3585 7075 160 Size: 872 1857 696 3490 1606 Fragments arranged by size: 3490 1857 1606 872 696 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8521 AvaI C'yCGr_G Cuts at: 1870 1943 5846 1870 Size: 73 3903 4545 Fragments arranged by size: 4545 3903 73 BamHI G'GATC_C Cuts at: 4530 5851 6218 4530 Size: 1321 367 6833 Fragments arranged by size: 6833 1321 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5842 7297 618 Size: 325 217 627 4055 1455 1842 Fragments arranged by size: 4055 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3126 3177 5466 5840 1391 Size: 1735 51 2289 374 4072 Fragments arranged by size: 4072 2289 1735 374 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2172 2214 928 Size: 971 273 42 7235 Fragments arranged by size: 7235 971 273 42 BbvCI CC'TCA_GC Cuts at: 3935 5453 3935 Size: 1518 7003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6712 6746 1903 Size: 34 50 34 4691 34 3678 Fragments arranged by size: 4691 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6413 7623 6413 Size: 1210 7311 BclI T'GATC_A Cuts at: 1548 4929 1548 Size: 3381 5140 BfrBI ATG'CAT Cuts at: 4219 4256 4219 Size: 37 8484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7132 136 Size: 107 122 71 631 605 5460 1525 Fragments arranged by size: 5460 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3280 3628 3748 4151 5788 8516 3280 Size: 348 120 403 1637 2728 3285 Fragments arranged by size: 3285 2728 1637 403 348 120 BlpI GC'TnA_GC Cuts at: 2013 5938 2013 Size: 3925 4596 Bme1580I G_kGCm'C Cuts at: 1790 2676 3826 5412 5466 6582 7738 1790 Size: 886 1150 1586 54 1116 1156 2573 Fragments arranged by size: 2573 1586 1156 1150 1116 886 54 BmgBI CAC'GTC Cuts at: 1318 5819 1318 Size: 4501 4020 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5480 6252 7210 Size: 416 53 83 399 24 4063 772 958 Cuts at: 7210 442 Size: 1753 Fragments arranged by size: 4063 1753 958 772 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3036 5769 3036 Size: 2733 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3110 3372 4140 4145 4263 4301 5709 7181 Size: 1871 262 768 5 118 38 1408 1472 Cuts at: 7181 1239 Size: 2579 Fragments arranged by size: 2579 1871 1472 1408 768 262 118 38 5 Bpu10I CC'TnA_GC Cuts at: 3250 3935 4743 5453 3250 Size: 685 808 710 6318 Fragments arranged by size: 6318 808 710 685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2485 3273 3584 3680 6646 7511 7809 2485 Size: 788 311 96 2966 865 298 3197 Fragments arranged by size: 3197 2966 865 788 311 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7184 882 Size: 617 5685 2219 Fragments arranged by size: 5685 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6312 493 Size: 711 5108 2702 Fragments arranged by size: 5108 2702 711 BsaBI GATnn'nnATC Cuts at: 4448 8515 4448 Size: 4067 4454 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6328 6710 7345 275 Size: 53 83 186 936 4795 382 635 1451 Fragments arranged by size: 4795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2458 4329 6954 7352 7626 7773 8184 2458 Size: 1871 2625 398 274 147 411 2795 Fragments arranged by size: 2795 2625 1871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5803 5833 1178 Size: 30 165 30 4400 30 3866 Fragments arranged by size: 4400 3866 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2777 4302 5192 5231 5827 1186 Size: 1591 1525 890 39 596 3880 Fragments arranged by size: 3880 1591 1525 890 596 39 BseYI C'CCAG_C Cuts at: 3439 5719 7724 8220 3439 Size: 2280 2005 496 3740 Fragments arranged by size: 3740 2280 2005 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7948 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5971 6732 7760 1540 Size: 275 186 3970 761 1028 2301 Fragments arranged by size: 3970 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2676 3126 3177 4512 5412 5779 6582 6667 7738 Size: 450 51 1335 900 367 803 85 1071 Cuts at: 7738 2676 Size: 3459 Fragments arranged by size: 3459 1335 1071 900 803 450 367 85 51 BsmI GAATG_Cn' Cuts at: 3153 4258 6032 6125 3153 Size: 1105 1774 93 5549 Fragments arranged by size: 5549 1774 1105 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5809 5809 Size: 8521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 3126 3177 3826 4512 5412 5466 Size: 399 886 450 51 649 686 900 54 Cuts at: 5466 5779 5840 6582 6667 7738 1391 Size: 313 61 742 85 1071 2174 Fragments arranged by size: 2174 1071 900 886 742 686 649 450 399 313 85 61 54 51 BspEI T'CCGG_A Cuts at: 2458 4329 8184 2458 Size: 1871 3855 2795 Fragments arranged by size: 3855 2795 1871 BspHI T'CATG_A Cuts at: 6410 8140 6410 Size: 1730 6791 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4990 5876 844 Size: 4146 886 3489 Fragments arranged by size: 4146 3489 886 BsrBI CCG'CTC Cuts at: 1394 1823 6408 1394 Size: 429 4585 3507 Fragments arranged by size: 4585 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 3774 3887 4221 7016 7190 65 Size: 1183 1359 1167 113 334 2795 174 1396 Fragments arranged by size: 2795 1396 1359 1183 1167 334 174 113 BsrFI r'CCGG_y Cuts at: 1700 1810 7165 7352 1700 Size: 110 5355 187 2869 Fragments arranged by size: 5355 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4341 96 Size: 4245 4276 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8521 BssSI C'ACGA_G Cuts at: 6581 7593 6581 Size: 1012 7509 BstAPI GCAn_nnn'nTGC Cuts at: 794 4216 5783 794 Size: 3422 1567 3532 Fragments arranged by size: 3532 3422 1567 BstBI TT'CG_AA Cuts at: 7325 7325 Size: 8521 BstEII G'GTnAC_C Cuts at: 1184 4360 1184 Size: 3176 5345 BstYI r'GATC_y Cuts at: 1972 3280 3628 3686 3748 3919 4151 4348 4530 Size: 1308 348 58 62 171 232 197 182 Cuts at: 4530 5556 5788 5851 6218 6603 6620 8072 8158 Size: 1026 232 63 367 385 17 1452 86 Cuts at: 8158 8170 8516 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1308 1026 385 367 348 346 232 232 197 182 171 86 63 62 58 17 12 Bsu36I CC'TnA_GG Cuts at: 4169 4169 Size: 8521 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7969 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7782 Fragments arranged by size: 7782 683 56 BtsI GCAGTG_nn' Cuts at: 2086 2133 4055 4621 6037 6831 6851 2086 Size: 47 1922 566 1416 794 20 3756 Fragments arranged by size: 3756 1922 1416 794 566 47 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8486 DraI TTT'AAA Cuts at: 3072 3454 4068 5825 3072 Size: 382 614 1757 5768 Fragments arranged by size: 5768 1757 614 382 DraIII CAC_nnn'GTG Cuts at: 4540 4540 Size: 8521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7528 809 Size: 507 6212 1802 Fragments arranged by size: 6212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4792 5968 6857 Size: 54 1425 50 275 18 2962 1176 889 Cuts at: 6857 8486 8 Size: 1629 43 Fragments arranged by size: 2962 1629 1425 1176 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5968 1537 Size: 275 4156 4090 Fragments arranged by size: 4156 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2171 2268 2298 2316 3833 5088 5169 5208 Size: 818 97 30 18 1517 1255 81 39 Cuts at: 5208 5217 5268 5619 6451 1353 Size: 9 51 351 832 3423 Fragments arranged by size: 3423 1517 1255 832 818 351 97 81 51 39 30 18 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2104 7108 7482 7628 387 Size: 1064 138 515 5004 374 146 1280 Fragments arranged by size: 5004 1280 1064 515 374 146 138 EcoICRI GAG'CTC Cuts at: 3124 3175 3124 Size: 51 8470 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2184 2470 2711 3110 3372 3645 4140 Size: 393 552 286 241 399 262 273 495 Cuts at: 4140 4145 4263 4301 5709 6584 7181 7968 8236 Size: 5 118 38 1408 875 597 787 268 Cuts at: 8236 1239 Size: 1524 Fragments arranged by size: 1524 1408 875 787 597 552 495 399 393 286 273 268 262 241 118 38 5 EcoO109I rG'GnC_Cy Cuts at: 2158 5462 5463 2158 Size: 3304 1 5216 Fragments arranged by size: 5216 3304 1 EcoRI G'AATT_C Cuts at: 5021 5830 6208 5021 Size: 809 378 7334 Fragments arranged by size: 7334 809 378 EcoRV GAT'ATC Cuts at: 2125 5659 2125 Size: 3534 4987 FspI TGC'GCA Cuts at: 7027 7027 Size: 8521 HaeII r_GCGC'y Cuts at: 1678 5719 7668 1678 Size: 4041 1949 2531 Fragments arranged by size: 4041 2531 1949 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2555 2587 2888 Size: 32 135 32 671 32 280 32 301 Cuts at: 2888 2920 3022 3054 3678 3710 4494 4526 4947 Size: 32 102 32 624 32 784 32 421 Cuts at: 4947 4979 5042 5074 5480 5512 7162 7194 7236 Size: 32 63 32 406 32 1650 32 42 Cuts at: 7236 7268 1373 Size: 32 2626 Fragments arranged by size: 2626 1650 784 671 624 421 406 301 280 135 102 63 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2942 3742 5526 5865 6111 669 Size: 988 281 1004 800 1784 339 246 3079 Fragments arranged by size: 3079 1784 1004 988 800 339 281 246 HindIII A'AGCT_T Cuts at: 748 2403 748 Size: 1655 6866 HpaI GTT'AAC Cuts at: 5526 6111 5526 Size: 585 7936 KpnI G_GTAC'C Cuts at: 5846 5846 Size: 8521 MfeI C'AATT_G Cuts at: 6120 6120 Size: 8521 MluI A'CGCG_T Cuts at: 8397 8397 Size: 8521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3513 3549 3831 7635 7819 1305 Size: 2208 36 282 3804 184 2007 Fragments arranged by size: 3804 2208 2007 282 184 36 MscI TGG'CCA Cuts at: 10 64 1489 1832 4794 10 Size: 54 1425 343 2962 3737 Fragments arranged by size: 3737 2962 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2913 3785 4224 6479 6838 6997 518 Size: 648 1747 872 439 2255 359 159 2042 Fragments arranged by size: 2255 2042 1747 872 648 439 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2357 4283 4664 5452 5937 6614 7762 Size: 406 345 1926 381 788 485 677 1148 Cuts at: 7762 8007 1606 Size: 245 2120 Fragments arranged by size: 2120 1926 1148 788 677 485 406 381 345 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7969 NdeI CA'TA_TG Cuts at: 387 387 Size: 8521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8521 NotI GC'GGCC_GC Cuts at: 5968 5968 Size: 8521 NsiI A_TGCA'T Cuts at: 4221 4258 4221 Size: 37 8484 NspI r_CATG'y Cuts at: 1132 1229 3904 4116 4134 4791 5318 5879 7424 Size: 97 2675 212 18 657 527 561 1545 Cuts at: 7424 1132 Size: 2229 Fragments arranged by size: 2675 2229 1545 657 561 527 212 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8521 PciI A'CATG_T Cuts at: 4130 4787 7420 4130 Size: 657 2633 5231 Fragments arranged by size: 5231 2633 657 PflMI CCAn_nnn'nTGG Cuts at: 1108 4540 1108 Size: 3432 5089 PfoI T'CCnGG_A Cuts at: 4317 4317 Size: 8521 PmeI GTTT'AAAC Cuts at: 5825 5825 Size: 8521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6590 6622 8103 8135 6590 Size: 32 1481 32 6976 Fragments arranged by size: 6976 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8521 PsiI TTA'TAA Cuts at: 6091 6091 Size: 8521 PspOMI G'GGCC_C Cuts at: 5462 5462 Size: 8521 PstI C_TGCA'G Cuts at: 830 2081 2998 5503 5873 830 Size: 1251 917 2505 370 3478 Fragments arranged by size: 3478 2505 1251 917 370 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8521 PvuII CAG'CTG Cuts at: 2012 2357 4283 4664 5452 2012 Size: 345 1926 381 788 5081 Fragments arranged by size: 5081 1926 788 381 345 SacI G_AGCT'C Cuts at: 3126 3177 3126 Size: 51 8470 SalI G'TCGA_C Cuts at: 1936 2940 5863 1936 Size: 1004 2923 4594 Fragments arranged by size: 4594 2923 1004 SapI GCTCTTCn'nnn_ Cuts at: 2316 2316 Size: 8521 SbfI CC_TGCA'GG Cuts at: 5873 5873 Size: 8521 ScaI AGT'ACT Cuts at: 3080 4799 6769 8481 3080 Size: 1719 1970 1712 3120 Fragments arranged by size: 3120 1970 1719 1712 SexAI A'CCwGG_T Cuts at: 3340 3340 Size: 8521 SfcI C'TryA_G Cuts at: 826 1045 2077 2619 2922 2994 3719 5378 5499 Size: 219 1032 542 303 72 725 1659 121 Cuts at: 5499 5869 7004 7685 7876 826 Size: 370 1135 681 191 1471 Fragments arranged by size: 1659 1471 1135 1032 725 681 542 370 303 219 191 121 72 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8521 SmaI CCC'GGG Cuts at: 1872 5848 1872 Size: 3976 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2500 3288 3563 3695 5309 6625 Size: 197 926 557 788 275 132 1614 1316 Cuts at: 6625 7526 7788 820 Size: 901 262 1553 Fragments arranged by size: 1614 1553 1316 926 901 788 557 275 262 197 132 SnaBI TAC'GTA Cuts at: 493 6312 493 Size: 5819 2702 SpeI A'CTAG_T Cuts at: 152 4504 152 Size: 4352 4169 SphI G_CATG'C Cuts at: 1132 4116 5879 1132 Size: 2984 1763 3774 Fragments arranged by size: 3774 2984 1763 SspI AAT'ATT Cuts at: 5 52 2884 3982 6445 5 Size: 47 2832 1098 2463 2081 Fragments arranged by size: 2832 2463 2081 1098 47 StuI AGG'CCT Cuts at: 1517 5040 1517 Size: 3523 4998 StyI C'CwwG_G Cuts at: 513 1065 2975 5960 513 Size: 552 1910 2985 3074 Fragments arranged by size: 3074 2985 1910 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6548 6565 6718 6903 7348 325 Size: 1255 4968 17 153 185 445 1498 Fragments arranged by size: 4968 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3078 4180 4341 Size: 274 80 33 51 1443 1101 1102 161 Cuts at: 4341 4797 6767 8479 96 Size: 456 1970 1712 138 Fragments arranged by size: 1970 1712 1443 1102 1101 456 274 161 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5803 5835 8481 8513 5803 Size: 32 2646 32 5811 Fragments arranged by size: 5811 2646 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8521 XbaI T'CTAG_A Cuts at: 3577 5857 3577 Size: 2280 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2582 4183 4523 4541 2582 Size: 1601 340 18 6562 Fragments arranged by size: 6562 1601 340 18 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8521 XmaI C'CCGG_G Cuts at: 1870 5846 1870 Size: 3976 4545 XmnI GAAnn'nnTTC Cuts at: 2385 4560 6650 2385 Size: 2175 2090 4256 Fragments arranged by size: 4256 2175 2090 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6329 276 Size: 53 83 186 936 4795 2468 Fragments arranged by size: 4795 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI