Restriction map for pFN21AA1000
 (Circular) (Six-base) MAPSORT of: pFN21AA1000.seq  Check: 9813  from: 1  to: 10543



pFN21AA1000  10543 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:37  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   6934    7039    6934 
   Size:        105   10438
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    8353     278 
   Size:         53      83     186     936    6817    2468

  Fragments arranged by size: 

                6817    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    4087    7886    1937 
   Size:       2150    3799    4594

  Fragments arranged by size: 

                4594    3799    2150
 
Acc65I G'GTAC_C

Cuts at:   3796    7864    3796 
   Size:       4068    6475
 
AclI AA'CG_TT

Cuts at:   1563    8670    9043    1563 
   Size:       7107     373    3063

  Fragments arranged by size: 

                7107    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2393    3039    4287    4551    4616    4691    4697    5135 
   Size:        761     646    1248     264      65      75       6     438

Cuts at:   5135    5336    5357    6870    6902    6962    7061    7067    7284 
   Size:        201      21    1513      32      60      99       6     217

Cuts at:   7284    7367    7455    7637    8606    9990   10258    1632 
   Size:         83      88     182     969    1384     268    1917

  Fragments arranged by size: 

                1917    1513    1384    1248     969     761     646     438
                 268     264     217     201     182      99      88      83
                  75      65      60      32      21       6       6
 
AfeI AGC'GCT

Cuts at:   5804    5804 
   Size:      10543
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   10346
 
AflIII A'CryG_T

Cuts at:   1203    3516    9442   10419    1203 
   Size:       2313    5926     977    1327

  Fragments arranged by size: 

                5926    2313    1327     977
 
AgeI A'CCGG_T

Cuts at:   9374    9374 
   Size:      10543
 
AhdI GACnn_n'nnGTC

Cuts at:   6325    9272    6325 
   Size:       2947    7596
 
AleI CACnn'nnGTG

Cuts at:   1166    2799    4325    1166 
   Size:       1633    1526    7384

  Fragments arranged by size: 

                7384    1633    1526
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    2817    4445    1184 
   Size:       1633    1628    7282

  Fragments arranged by size: 

                7282    1633    1628
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3705    3737    3705 
   Size:         32   10511
 
AlwNI CAG_nnn'CTG

Cuts at:   2996    5059    5560    5689    8286    9858    2996 
   Size:       2063     501     129    2597    1572    3681

  Fragments arranged by size: 

                3681    2597    2063    1572     501     129
 
ApaI G_GGCC'C

Cuts at:   3379    3379 
   Size:      10543
 
ApaLI G'TGCA_C

Cuts at:   4608    8600    9756    4608 
   Size:       3992    1156    5395

  Fragments arranged by size: 

                5395    3992    1156
 
ApoI r'AATT_y

Cuts at:   1493    2780    2907    3128    3755    4439    4837    5311    5549 
   Size:       1287     127     221     627     684     398     474     238

Cuts at:   5549    6793    7852    8078    8230   10339    1493 
   Size:       1244    1059     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1287    1244    1059     684     627     474
                 398     238     226     221     152     127
 
AseI AT'TA_AT

Cuts at:    160    1032    3474    9097     160 
   Size:        872    2442    5623    1606

  Fragments arranged by size: 

                5623    2442    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10543
 
AvaI C'yCGr_G

Cuts at:   1870    1943    5424    5648    6314    6335    7868    1870 
   Size:         73    3481     224     666      21    1533    4545

  Fragments arranged by size: 

                4545    3481    1533     666     224      73      21
 
BamHI G'GATC_C

Cuts at:   5504    7873    8240    5504 
   Size:       2369     367    7807

  Fragments arranged by size: 

                7807    2369     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2801    3314    3796    5147    6294 
   Size:        325     217     627    1014     513     482    1351    1147

Cuts at:   6294    7864    9319     618 
   Size:       1570    1455    1842

  Fragments arranged by size: 

                1842    1570    1455    1351    1147    1014     627     513
                 482     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3379    5649    6315    6483    6691    7158    7561    7862 
   Size:       1988    2270     666     168     208     467     403     301

Cuts at:   7862    1391 
   Size:       4072

  Fragments arranged by size: 

                4072    2270    1988     666     467     403     301     208
                 168
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2328    3867    4384    5363    5985    6198    6656 
   Size:        971     429    1539     517     979     622     213     458

Cuts at:   6656    7614     928 
   Size:        958    3857

  Fragments arranged by size: 

                3857    1539     979     971     958     622     517     458
                 429     213
 
BbvCI CC'TCA_GC

Cuts at:   2202    5279    7211    2202 
   Size:       3077    1932    5534

  Fragments arranged by size: 

                5534    3077    1932
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    8734    8768    1903 
   Size:         34    6797      34    3678

  Fragments arranged by size: 

                6797    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4684    8435    9645    4684 
   Size:       3751    1210    5582

  Fragments arranged by size: 

                5582    3751    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:      10543
 
BfrBI ATG'CAT

Cuts at:   4056    7578    4056 
   Size:       3522    7021
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    7310    7322    9154 
   Size:        107     122      71     631     605    5638      12    1832

Cuts at:   9154     136 
   Size:       1525

  Fragments arranged by size: 

                5638    1832    1525     631     605     122     107      71
                  12
 
BglII A'GATC_T

Cuts at:   3604   10538    3604 
   Size:       6934    3609
 
BlpI GC'TnA_GC

Cuts at:   2118    4719    5090    5346    5517    5643    6048    6105    6926 
   Size:       2601     371     256     171     126     405      57     821

Cuts at:   6926    7062    7960    2118 
   Size:        136     898    4701

  Fragments arranged by size: 

                4701    2601     898     821     405     371     256     171
                 136     126      57
 
Bme1580I G_kGCm'C

Cuts at:   1790    3319    3379    4612    5152    6025    8604    9760    1790 
   Size:       1529      60    1233     540     873    2579    1156    2573

  Fragments arranged by size: 

                2579    2573    1529    1233    1156     873     540      60
            
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:      10543
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4297    8274    9232 
   Size:        416      53      83     399      24    2880    3977     958

Cuts at:   9232     442 
   Size:       1753

  Fragments arranged by size: 

                3977    2880    1753     958     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:      10543
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   5471    5471 
   Size:      10543
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2286    2898    3120    3548    3713    4805    5225    5510 
   Size:       1047     612     222     428     165    1092     420     285

Cuts at:   5510    6329    6905    7250    7538    9203    1239 
   Size:        819     576     345     288    1665    2579

  Fragments arranged by size: 

                2579    1665    1092    1047     819     612     576     428
                 420     345     288     285     222     165
 
Bpu10I CC'TnA_GC

Cuts at:   2202    5099    5279    5763    7211    2202 
   Size:       2897     180     484    1448    5534

  Fragments arranged by size: 

                5534    2897    1448     484     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3464    3479    5162    5174    6470    6476    7589    7770    8668 
   Size:         15    1683      12    1296       6    1113     181     898

Cuts at:   8668    9533    9831    3464 
   Size:        865     298    4176

  Fragments arranged by size: 

                4176    1683    1296    1113     898     865     298     181
                  15      12       6
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2420    5999    9206     882 
   Size:        617     921    3579    3207    2219

  Fragments arranged by size: 

                3579    3207    2219     921     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    8334     493 
   Size:        711    7130    2702

  Fragments arranged by size: 

                7130    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   4433    6986   10537    4433 
   Size:       2553    3551    4439

  Fragments arranged by size: 

                4439    3551    2553
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    6321    8350    8732    9367 
   Size:         53      83     186     936    4788    2029     382     635

Cuts at:   9367     275 
   Size:       1451

  Fragments arranged by size: 

                4788    2029    1451     936     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   6114    8976    9374    9648    9795   10206    6114 
   Size:       2862     398     274     147     411    6451

  Fragments arranged by size: 

                6451    2862     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2963    2993    4569    4599    5875 
   Size:         30     165      30    1560      30    1576      30    1276

Cuts at:   5875    5905    1178 
   Size:         30    5816

  Fragments arranged by size: 

                5816    1576    1560    1276     165      30      30      30
                  30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2755    3205    4844    5093    5096    5507    6437    6473 
   Size:       1569     450    1639     249       3     411     930      36

Cuts at:   6473    6521    7199    1186 
   Size:         48     678    4530

  Fragments arranged by size: 

                4530    1639    1569     930     678     450     411     249
                  48      36       3
 
BseYI C'CCAG_C

Cuts at:   4340    9746   10242    4340 
   Size:       5406     496    4641

  Fragments arranged by size: 

                5406    4641     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    5054    5057    5180    5546    5573    6095    7295 
   Size:        573    3318       3     123     366      27     522    1200

Cuts at:   7295    7560    1163 
   Size:        265    4146

  Fragments arranged by size: 

                4146    3318    1200     573     522     366     265     123
                  27       3
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    7993    8754    9782    1540 
   Size:        275     186    5992     761    1028    2301

  Fragments arranged by size: 

                5992    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4417    4612    4959    5649    6315    6483    7158    8604    8689 
   Size:        195     347     690     666     168     675    1446      85

Cuts at:   8689    9760    4417 
   Size:       1071    5200

  Fragments arranged by size: 

                5200    1446    1071     690     675     666     347     195
                 168      85
 
BsmI GAATG_Cn'

Cuts at:   2191    3414    4209    8054    8147    2191 
   Size:       1223     795    3845      93    4587

  Fragments arranged by size: 

                4587    3845    1223     795      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    3319    3379    4417    4612    4959    5152    5649 
   Size:        399    1529      60    1038     195     347     193     497

Cuts at:   5649    6025    6315    6483    6691    7158    7561    7862    8604 
   Size:        376     290     168     208     467     403     301     742

Cuts at:   8604    8689    9760    1391 
   Size:         85    1071    2174

  Fragments arranged by size: 

                2174    1529    1071    1038     742     497     467     403
                 399     376     347     301     290     208     195     193
                 168      85      60
 
BspEI T'CCGG_A

Cuts at:  10206   10206 
   Size:      10543
 
BspHI T'CATG_A

Cuts at:   2932    8432   10162    2932 
   Size:       5500    1730    3313

  Fragments arranged by size: 

                5500    3313    1730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2946    5076    5665    6934    7039    7898     844 
   Size:       2102    2130     589    1269     105     859    3489

  Fragments arranged by size: 

                3489    2130    2102    1269     859     589     105
 
BsrBI CCG'CTC

Cuts at:   1394    1823    6348    7066    8430    1394 
   Size:        429    4525     718    1364    3507

  Fragments arranged by size: 

                4525    3507    1364     718     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2337    6155    7365    9038    9212      65 
   Size:       1183    1089    3818    1210    1673     174    1396

  Fragments arranged by size: 

                3818    1673    1396    1210    1183    1089     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    6939    9187    9374    1700 
   Size:        110    5129    2248     187    2869

  Fragments arranged by size: 

                5129    2869    2248     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:      10543
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10543
 
BssSI C'ACGA_G

Cuts at:   6419    8603    9615    6419 
   Size:       2184    1012    7347

  Fragments arranged by size: 

                7347    2184    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    6301     794 
   Size:       5507    5036
 
BstBI TT'CG_AA

Cuts at:   9347    9347 
   Size:      10543
 
BstEII G'GTnAC_C

Cuts at:   1184    3298    6871    1184 
   Size:       2114    3573    4856

  Fragments arranged by size: 

                4856    3573    2114
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2146    2876    6256    6967    2146 
   Size:        730    3380     711    5722

  Fragments arranged by size: 

                5722    3380     730     711
 
BstYI r'GATC_y

Cuts at:   1972    2068    3604    4776    5504    7099    7873    8240    8625 
   Size:         96    1536    1172     728    1595     774     367     385

Cuts at:   8625    8642   10094   10180   10192   10538    1972 
   Size:         17    1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1595    1536    1452    1172     774     728     385
                 367     346      96      86      17      12
 
BstZ17I GTA'TAC

Cuts at:   4088    4088 
   Size:      10543
 
Bsu36I CC'TnA_GG

Cuts at:   3663    3663 
   Size:      10543
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    3045    6249     513 
   Size:        552     939    1041    3204    4807

  Fragments arranged by size: 

                4807    3204    1041     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    7588     487 
   Size:        683      56    6362    3442

  Fragments arranged by size: 

                6362    3442     683      56
 
BtsI GCAGTG_nn'

Cuts at:   4757    6818    7543    8059    8853    8873    4757 
   Size:       2061     725     516     794      20    6427

  Fragments arranged by size: 

                6427    2061     794     725     516      20
 
ClaI AT'CG_AT

Cuts at:   1554    4902    1554 
   Size:       3348    7195
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35   10508
 
DraI TTT'AAA

Cuts at:   3133    4195    4336    5217    7847    3133 
   Size:       1062     141     881    2630    5829

  Fragments arranged by size: 

                5829    2630    1062     881     141
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    9550     809 
   Size:        507    8234    1802

  Fragments arranged by size: 

                8234    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2386    2475    3030 
   Size:         54    1425      50     275      18     556      89     555

Cuts at:   3030    6258    7132    7990    8879   10508       8 
   Size:       3228     874     858     889    1629      43

  Fragments arranged by size: 

                3228    1629    1425     889     874     858     556     555
                 275      89      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    7990    1537 
   Size:        275    6178    4090

  Fragments arranged by size: 

                6178    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2423    2486    2588    3144    3642    4541    4596    4740 
   Size:       1070      63     102     556     498     899      55     144

Cuts at:   4740    4821    5268    5571    5615    5793    5910    6555    6639 
   Size:         81     447     303      44     178     117     645      84

Cuts at:   6639    6891    7083    7146    7260    7290    8473    1353 
   Size:        252     192      63     114      30    1183    3423

  Fragments arranged by size: 

                3423    1183    1070     899     645     556     498     447
                 303     252     192     178     144     117     114     102
                  84      81      63      63      55      44      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4876    7709    9130    9504    9650     387 
   Size:       1064     138    3287    2833    1421     374     146    1280

  Fragments arranged by size: 

                3287    2833    1421    1280    1064     374     146     138
            
 
EcoICRI GAG'CTC

Cuts at:   5647    6313    6481    7156    5647 
   Size:        666     168     675    9034

  Fragments arranged by size: 

                9034     675     666     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2286    2393    2898    3039    3120    3548    3713 
   Size:        393     654     107     505     141      81     428     165

Cuts at:   3713    4287    4551    4616    4691    4697    4805    5135    5225 
   Size:        574     264      65      75       6     108     330      90

Cuts at:   5225    5336    5357    5510    6329    6870    6902    6905    6962 
   Size:        111      21     153     819     541      32       3      57

Cuts at:   6962    7061    7067    7250    7284    7367    7455    7538    7637 
   Size:         99       6     183      34      83      88      83      99

Cuts at:   7637    8606    9203    9990   10258    1239 
   Size:        969     597     787     268    1524

  Fragments arranged by size: 

                1524     969     819     787     654     597     574     541
                 505     428     393     330     268     264     183     165
                 153     141     111     108     107      99      99      90
                  88      83      83      81      75      65      57      34
                  32      21       6       6       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2348    4528    4682    5156    6041    6338    2348 
   Size:       2180     154     474     885     297    6553

  Fragments arranged by size: 

                6553    2180     885     474     297     154
 
EcoO109I rG'GnC_Cy

Cuts at:   2034    3375    3376    4224    6394    6901    6994    7107    2034 
   Size:       1341       1     848    2170     507      93     113    5470

  Fragments arranged by size: 

                5470    2170    1341     848     507     113      93       1
            
 
EcoRI G'AATT_C

Cuts at:   5311    7852    8230    5311 
   Size:       2541     378    7624

  Fragments arranged by size: 

                7624    2541     378
 
EcoRV GAT'ATC

Cuts at:   2836    5005    2836 
   Size:       2169    8374
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3857    4292    5725    5977    6770    3857 
   Size:        435    1433     252     793    7630

  Fragments arranged by size: 

                7630    1433     793     435     252
 
FspI TGC'GCA

Cuts at:   9049    9049 
   Size:      10543
 
HaeII r_GCGC'y

Cuts at:   1678    2383    5806    9690    1678 
   Size:        705    3423    3884    2531

  Fragments arranged by size: 

                3884    3423    2531     705
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2284    2316    2750    2782    4006 
   Size:         32     135      32     712      32     434      32    1224

Cuts at:   4006    4038    4302    4334    4367    4399    4537    4569    5996 
   Size:         32     264      32      33      32     138      32    1427

Cuts at:   5996    6028    6311    6343    6556    6588    7091    7123    7621 
   Size:         32     283      32     213      32     503      32     498

Cuts at:   7621    7653    9184    9216    9258    9290    1373 
   Size:         32    1531      32      42      32    2626

  Fragments arranged by size: 

                2626    1531    1427    1224     712     503     498     434
                 283     264     213     138     135      42      33      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    7887    8133     669 
   Size:        988     281    5949     246    3079

  Fragments arranged by size: 

                5949    3079     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3594    6764     748 
   Size:       2846    3170    4527

  Fragments arranged by size: 

                4527    3170    2846
 
HpaI GTT'AAC

Cuts at:   8133    8133 
   Size:      10543
 
KpnI G_GTAC'C

Cuts at:   3800    7868    3800 
   Size:       4068    6475
 
MfeI C'AATT_G

Cuts at:   3508    8142    3508 
   Size:       4634    5909
 
MluI A'CGCG_T

Cuts at:  10419   10419 
   Size:      10543
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3903    4788    9657    9841    1305 
   Size:       2598     885    4869     184    2007

  Fragments arranged by size: 

                4869    2598    2007     885     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2388    3032    6260      10 
   Size:         54    1425     343     556     644    3228    4293

  Fragments arranged by size: 

                4293    3228    1425     644     556     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2330    2346    2789    2799    4325    4508    6254 
   Size:        648    1164      16     443      10    1526     183    1746

Cuts at:   6254    8501    8860    9019     518 
   Size:       2247     359     159    2042

  Fragments arranged by size: 

                2247    2042    1746    1526    1164     648     443     359
                 183     159      16      10
 
MspA1I CmG'CkG

Cuts at:   1606    4265    4783    5521    5995    6301    6307    6379    7009 
   Size:       2659     518     738     474     306       6      72     630

Cuts at:   7009    7959    8636    9784   10029    1606 
   Size:        950     677    1148     245    2120

  Fragments arranged by size: 

                2659    2120    1148     950     738     677     630     518
                 474     306     245      72       6
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    3045    6249     513 
   Size:        552     939    1041    3204    4807

  Fragments arranged by size: 

                4807    3204    1041     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:      10543
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:      10543
 
NotI GC'GGCC_GC

Cuts at:   7990    7990 
   Size:      10543
 
NsiI A_TGCA'T

Cuts at:   4058    7580    4058 
   Size:       3522    7021
 
NspI r_CATG'y

Cuts at:   1132    1229    2554    2815    3520    5630    7901    9446    1132 
   Size:         97    1325     261     705    2110    2271    1545    2229

  Fragments arranged by size: 

                2271    2229    2110    1545    1325     705     261      97
            
 
PasI CC'CwG_GG

Cuts at:   1348    6967    1348 
   Size:       5619    4924
 
PciI A'CATG_T

Cuts at:   3516    9442    3516 
   Size:       5926    4617
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    5467    1108 
   Size:       4359    6184
 
PfoI T'CCnGG_A

Cuts at:   2669    7158    2669 
   Size:       4489    6054
 
PmeI GTTT'AAAC

Cuts at:   7847    7847 
   Size:      10543
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   8612    8644   10125   10157    8612 
   Size:         32    1481      32    8998

  Fragments arranged by size: 

                8998    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   4224    6901    6994    7107    4224 
   Size:       2677      93     113    7660

  Fragments arranged by size: 

                7660    2677     113      93
 
PshAI GACnn'nnGTC

Cuts at:   1613    6656    1613 
   Size:       5043    5500
 
PsiI TTA'TAA

Cuts at:   3804    8113    3804 
   Size:       4309    6234
 
PspOMI G'GGCC_C

Cuts at:   3375    3375 
   Size:      10543
 
PspXI vC'TCGA_Gb

Cuts at:   5424    5648    5424 
   Size:        224   10319
 
PstI C_TGCA'G

Cuts at:    830    4182    6042    6306    6355    6384    6834    7895     830 
   Size:       3352    1860     264      49      29     450    1061    3478

  Fragments arranged by size: 

                3478    3352    1860    1061     450     264      49      29
            
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10543
 
PvuII CAG'CTG

Cuts at:   4265    4783    5521    5995    6301    6307    6379    7009    4265 
   Size:        518     738     474     306       6      72     630    7799

  Fragments arranged by size: 

                7799     738     630     518     474     306      72       6
            
 
SacI G_AGCT'C

Cuts at:   5649    6315    6483    7158    5649 
   Size:        666     168     675    9034

  Fragments arranged by size: 

                9034     675     666     168
 
SalI G'TCGA_C

Cuts at:   1936    7885    1936 
   Size:       5949    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   3144    4541    4740    5268    5793    7146    3144 
   Size:       1397     199     528     525    1353    6541

  Fragments arranged by size: 

                6541    1397    1353     528     525     199
 
SbfI CC_TGCA'GG

Cuts at:   7895    7895 
   Size:      10543
 
ScaI AGT'ACT

Cuts at:   3919    8791   10503    3919 
   Size:       4872    1712    3959

  Fragments arranged by size: 

                4872    3959    1712
 
SexAI A'CCwGG_T

Cuts at:   4081    6582    4081 
   Size:       2501    8042
 
SfcI C'TryA_G

Cuts at:    826    1045    2132    3288    3669    4109    4178    4950    5669 
   Size:        219    1087    1156     381     440      69     772     719

Cuts at:   5669    6038    6302    6351    6380    6830    7891    9026    9707 
   Size:        369     264      49      29     450    1061    1135     681

Cuts at:   9707    9898     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1156    1135    1087    1061     772     719     681
                 450     440     381     369     264     219     191      69
                  49      29
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10543
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:      10543
 
SmaI CCC'GGG

Cuts at:   1872    7870    1872 
   Size:       5998    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3443    3458    5141    5153    5424    5648 
   Size:        197     926    1500      15    1683      12     271     224

Cuts at:   5648    6485    6491    7568    7785    8647    9548    9810     820 
   Size:        837       6    1077     217     862     901     262    1553

  Fragments arranged by size: 

                1683    1553    1500    1077     926     901     862     837
                 271     262     224     217     197      15      12       6
            
 
SnaBI TAC'GTA

Cuts at:    493    8334     493 
   Size:       7841    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:      10543
 
SphI G_CATG'C

Cuts at:   1132    2815    7901    1132 
   Size:       1683    5086    3774

  Fragments arranged by size: 

                5086    3774    1683
 
SspI AAT'ATT

Cuts at:      5      52    8467       5 
   Size:         47    8415    2081

  Fragments arranged by size: 

                8415    2081      47
 
StuI AGG'CCT

Cuts at:   1517    5337    1517 
   Size:       3820    6723
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2080    2145    3045    4220    4284    4626 
   Size:        552     939      76      65     900    1175      64     342

Cuts at:   4626    5028    6249    6279    7982     513 
   Size:        402    1221      30    1703    3074

  Fragments arranged by size: 

                3074    1703    1221    1175     939     900     552     402
                 342      76      65      64      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3553    5474    8570    8587    8740    8925    9370 
   Size:       1255    1973    1921    3096      17     153     185     445

Cuts at:   9370     325 
   Size:       1498

  Fragments arranged by size: 

                3096    1973    1921    1498    1255     445     185     153
                  17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3917    5786    6473 
   Size:        274      80      33      51    1443    1940    1869     687

Cuts at:   6473    8789   10501      96 
   Size:       2316    1712     138

  Fragments arranged by size: 

                2316    1940    1869    1712    1443     687     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2598    2630    2963    2995    4141    4173    5875    5907    6410 
   Size:         32     333      32    1146      32    1702      32     503

Cuts at:   6410    6442   10503   10535    2598 
   Size:         32    4061      32    2606

  Fragments arranged by size: 

                4061    2606    1702    1146     503     333      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:      10543
 
XbaI T'CTAG_A

Cuts at:   7879    7879 
   Size:      10543
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3052    3886    4310    4780    6214    7372    3052 
   Size:        834     424     470    1434    1158    6223

  Fragments arranged by size: 

                6223    1434    1158     834     470     424
 
XhoI C'TCGA_G

Cuts at:   1943    5424    5648    1943 
   Size:       3481     224    6838

  Fragments arranged by size: 

                6838    3481     224
 
XmaI C'CCGG_G

Cuts at:   1870    7868    1870 
   Size:       5998    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4164    6766    8672    4164 
   Size:       2602    1906    6035

  Fragments arranged by size: 

                6035    2602    1906
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    8351     276 
   Size:         53      83     186     936    6817    2468

  Fragments arranged by size: 

                6817    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AscI    AvrII     BaeI     BbeI    BsiWI    BsmBI   DraIII     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI
     PsrI    RsrII    SacII    SanDI     SfiI     SfoI     SrfI     SwaI
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