(Circular) (Six-base) MAPSORT of: pFN21AA1000.seq Check: 9813 from: 1 to: 10543 pFN21AA1000 10543 bp With 182 enzymes: SgfI * February 27, 2009 14:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 6934 7039 6934 Size: 105 10438 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8353 278 Size: 53 83 186 936 6817 2468 Fragments arranged by size: 6817 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4087 7886 1937 Size: 2150 3799 4594 Fragments arranged by size: 4594 3799 2150 Acc65I G'GTAC_C Cuts at: 3796 7864 3796 Size: 4068 6475 AclI AA'CG_TT Cuts at: 1563 8670 9043 1563 Size: 7107 373 3063 Fragments arranged by size: 7107 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2393 3039 4287 4551 4616 4691 4697 5135 Size: 761 646 1248 264 65 75 6 438 Cuts at: 5135 5336 5357 6870 6902 6962 7061 7067 7284 Size: 201 21 1513 32 60 99 6 217 Cuts at: 7284 7367 7455 7637 8606 9990 10258 1632 Size: 83 88 182 969 1384 268 1917 Fragments arranged by size: 1917 1513 1384 1248 969 761 646 438 268 264 217 201 182 99 88 83 75 65 60 32 21 6 6 AfeI AGC'GCT Cuts at: 5804 5804 Size: 10543 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10346 AflIII A'CryG_T Cuts at: 1203 3516 9442 10419 1203 Size: 2313 5926 977 1327 Fragments arranged by size: 5926 2313 1327 977 AgeI A'CCGG_T Cuts at: 9374 9374 Size: 10543 AhdI GACnn_n'nnGTC Cuts at: 6325 9272 6325 Size: 2947 7596 AleI CACnn'nnGTG Cuts at: 1166 2799 4325 1166 Size: 1633 1526 7384 Fragments arranged by size: 7384 1633 1526 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2817 4445 1184 Size: 1633 1628 7282 Fragments arranged by size: 7282 1633 1628 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3705 3737 3705 Size: 32 10511 AlwNI CAG_nnn'CTG Cuts at: 2996 5059 5560 5689 8286 9858 2996 Size: 2063 501 129 2597 1572 3681 Fragments arranged by size: 3681 2597 2063 1572 501 129 ApaI G_GGCC'C Cuts at: 3379 3379 Size: 10543 ApaLI G'TGCA_C Cuts at: 4608 8600 9756 4608 Size: 3992 1156 5395 Fragments arranged by size: 5395 3992 1156 ApoI r'AATT_y Cuts at: 1493 2780 2907 3128 3755 4439 4837 5311 5549 Size: 1287 127 221 627 684 398 474 238 Cuts at: 5549 6793 7852 8078 8230 10339 1493 Size: 1244 1059 226 152 2109 1697 Fragments arranged by size: 2109 1697 1287 1244 1059 684 627 474 398 238 226 221 152 127 AseI AT'TA_AT Cuts at: 160 1032 3474 9097 160 Size: 872 2442 5623 1606 Fragments arranged by size: 5623 2442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10543 AvaI C'yCGr_G Cuts at: 1870 1943 5424 5648 6314 6335 7868 1870 Size: 73 3481 224 666 21 1533 4545 Fragments arranged by size: 4545 3481 1533 666 224 73 21 BamHI G'GATC_C Cuts at: 5504 7873 8240 5504 Size: 2369 367 7807 Fragments arranged by size: 7807 2369 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2801 3314 3796 5147 6294 Size: 325 217 627 1014 513 482 1351 1147 Cuts at: 6294 7864 9319 618 Size: 1570 1455 1842 Fragments arranged by size: 1842 1570 1455 1351 1147 1014 627 513 482 325 217 BanII G_rGCy'C Cuts at: 1391 3379 5649 6315 6483 6691 7158 7561 7862 Size: 1988 2270 666 168 208 467 403 301 Cuts at: 7862 1391 Size: 4072 Fragments arranged by size: 4072 2270 1988 666 467 403 301 208 168 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 3867 4384 5363 5985 6198 6656 Size: 971 429 1539 517 979 622 213 458 Cuts at: 6656 7614 928 Size: 958 3857 Fragments arranged by size: 3857 1539 979 971 958 622 517 458 429 213 BbvCI CC'TCA_GC Cuts at: 2202 5279 7211 2202 Size: 3077 1932 5534 Fragments arranged by size: 5534 3077 1932 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8734 8768 1903 Size: 34 6797 34 3678 Fragments arranged by size: 6797 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4684 8435 9645 4684 Size: 3751 1210 5582 Fragments arranged by size: 5582 3751 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 10543 BfrBI ATG'CAT Cuts at: 4056 7578 4056 Size: 3522 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7310 7322 9154 Size: 107 122 71 631 605 5638 12 1832 Cuts at: 9154 136 Size: 1525 Fragments arranged by size: 5638 1832 1525 631 605 122 107 71 12 BglII A'GATC_T Cuts at: 3604 10538 3604 Size: 6934 3609 BlpI GC'TnA_GC Cuts at: 2118 4719 5090 5346 5517 5643 6048 6105 6926 Size: 2601 371 256 171 126 405 57 821 Cuts at: 6926 7062 7960 2118 Size: 136 898 4701 Fragments arranged by size: 4701 2601 898 821 405 371 256 171 136 126 57 Bme1580I G_kGCm'C Cuts at: 1790 3319 3379 4612 5152 6025 8604 9760 1790 Size: 1529 60 1233 540 873 2579 1156 2573 Fragments arranged by size: 2579 2573 1529 1233 1156 873 540 60 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10543 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4297 8274 9232 Size: 416 53 83 399 24 2880 3977 958 Cuts at: 9232 442 Size: 1753 Fragments arranged by size: 3977 2880 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10543 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5471 5471 Size: 10543 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2898 3120 3548 3713 4805 5225 5510 Size: 1047 612 222 428 165 1092 420 285 Cuts at: 5510 6329 6905 7250 7538 9203 1239 Size: 819 576 345 288 1665 2579 Fragments arranged by size: 2579 1665 1092 1047 819 612 576 428 420 345 288 285 222 165 Bpu10I CC'TnA_GC Cuts at: 2202 5099 5279 5763 7211 2202 Size: 2897 180 484 1448 5534 Fragments arranged by size: 5534 2897 1448 484 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3464 3479 5162 5174 6470 6476 7589 7770 8668 Size: 15 1683 12 1296 6 1113 181 898 Cuts at: 8668 9533 9831 3464 Size: 865 298 4176 Fragments arranged by size: 4176 1683 1296 1113 898 865 298 181 15 12 6 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 5999 9206 882 Size: 617 921 3579 3207 2219 Fragments arranged by size: 3579 3207 2219 921 617 BsaAI yAC'GTr Cuts at: 493 1204 8334 493 Size: 711 7130 2702 Fragments arranged by size: 7130 2702 711 BsaBI GATnn'nnATC Cuts at: 4433 6986 10537 4433 Size: 2553 3551 4439 Fragments arranged by size: 4439 3551 2553 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6321 8350 8732 9367 Size: 53 83 186 936 4788 2029 382 635 Cuts at: 9367 275 Size: 1451 Fragments arranged by size: 4788 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6114 8976 9374 9648 9795 10206 6114 Size: 2862 398 274 147 411 6451 Fragments arranged by size: 6451 2862 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2963 2993 4569 4599 5875 Size: 30 165 30 1560 30 1576 30 1276 Cuts at: 5875 5905 1178 Size: 30 5816 Fragments arranged by size: 5816 1576 1560 1276 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2755 3205 4844 5093 5096 5507 6437 6473 Size: 1569 450 1639 249 3 411 930 36 Cuts at: 6473 6521 7199 1186 Size: 48 678 4530 Fragments arranged by size: 4530 1639 1569 930 678 450 411 249 48 36 3 BseYI C'CCAG_C Cuts at: 4340 9746 10242 4340 Size: 5406 496 4641 Fragments arranged by size: 5406 4641 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5054 5057 5180 5546 5573 6095 7295 Size: 573 3318 3 123 366 27 522 1200 Cuts at: 7295 7560 1163 Size: 265 4146 Fragments arranged by size: 4146 3318 1200 573 522 366 265 123 27 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7993 8754 9782 1540 Size: 275 186 5992 761 1028 2301 Fragments arranged by size: 5992 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4417 4612 4959 5649 6315 6483 7158 8604 8689 Size: 195 347 690 666 168 675 1446 85 Cuts at: 8689 9760 4417 Size: 1071 5200 Fragments arranged by size: 5200 1446 1071 690 675 666 347 195 168 85 BsmI GAATG_Cn' Cuts at: 2191 3414 4209 8054 8147 2191 Size: 1223 795 3845 93 4587 Fragments arranged by size: 4587 3845 1223 795 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3319 3379 4417 4612 4959 5152 5649 Size: 399 1529 60 1038 195 347 193 497 Cuts at: 5649 6025 6315 6483 6691 7158 7561 7862 8604 Size: 376 290 168 208 467 403 301 742 Cuts at: 8604 8689 9760 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1529 1071 1038 742 497 467 403 399 376 347 301 290 208 195 193 168 85 60 BspEI T'CCGG_A Cuts at: 10206 10206 Size: 10543 BspHI T'CATG_A Cuts at: 2932 8432 10162 2932 Size: 5500 1730 3313 Fragments arranged by size: 5500 3313 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2946 5076 5665 6934 7039 7898 844 Size: 2102 2130 589 1269 105 859 3489 Fragments arranged by size: 3489 2130 2102 1269 859 589 105 BsrBI CCG'CTC Cuts at: 1394 1823 6348 7066 8430 1394 Size: 429 4525 718 1364 3507 Fragments arranged by size: 4525 3507 1364 718 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2337 6155 7365 9038 9212 65 Size: 1183 1089 3818 1210 1673 174 1396 Fragments arranged by size: 3818 1673 1396 1210 1183 1089 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6939 9187 9374 1700 Size: 110 5129 2248 187 2869 Fragments arranged by size: 5129 2869 2248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 10543 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10543 BssSI C'ACGA_G Cuts at: 6419 8603 9615 6419 Size: 2184 1012 7347 Fragments arranged by size: 7347 2184 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6301 794 Size: 5507 5036 BstBI TT'CG_AA Cuts at: 9347 9347 Size: 10543 BstEII G'GTnAC_C Cuts at: 1184 3298 6871 1184 Size: 2114 3573 4856 Fragments arranged by size: 4856 3573 2114 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2876 6256 6967 2146 Size: 730 3380 711 5722 Fragments arranged by size: 5722 3380 730 711 BstYI r'GATC_y Cuts at: 1972 2068 3604 4776 5504 7099 7873 8240 8625 Size: 96 1536 1172 728 1595 774 367 385 Cuts at: 8625 8642 10094 10180 10192 10538 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1595 1536 1452 1172 774 728 385 367 346 96 86 17 12 BstZ17I GTA'TAC Cuts at: 4088 4088 Size: 10543 Bsu36I CC'TnA_GG Cuts at: 3663 3663 Size: 10543 BtgI C'CryG_G Cuts at: 513 1065 2004 3045 6249 513 Size: 552 939 1041 3204 4807 Fragments arranged by size: 4807 3204 1041 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 7588 487 Size: 683 56 6362 3442 Fragments arranged by size: 6362 3442 683 56 BtsI GCAGTG_nn' Cuts at: 4757 6818 7543 8059 8853 8873 4757 Size: 2061 725 516 794 20 6427 Fragments arranged by size: 6427 2061 794 725 516 20 ClaI AT'CG_AT Cuts at: 1554 4902 1554 Size: 3348 7195 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10508 DraI TTT'AAA Cuts at: 3133 4195 4336 5217 7847 3133 Size: 1062 141 881 2630 5829 Fragments arranged by size: 5829 2630 1062 881 141 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9550 809 Size: 507 8234 1802 Fragments arranged by size: 8234 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2386 2475 3030 Size: 54 1425 50 275 18 556 89 555 Cuts at: 3030 6258 7132 7990 8879 10508 8 Size: 3228 874 858 889 1629 43 Fragments arranged by size: 3228 1629 1425 889 874 858 556 555 275 89 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7990 1537 Size: 275 6178 4090 Fragments arranged by size: 6178 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2486 2588 3144 3642 4541 4596 4740 Size: 1070 63 102 556 498 899 55 144 Cuts at: 4740 4821 5268 5571 5615 5793 5910 6555 6639 Size: 81 447 303 44 178 117 645 84 Cuts at: 6639 6891 7083 7146 7260 7290 8473 1353 Size: 252 192 63 114 30 1183 3423 Fragments arranged by size: 3423 1183 1070 899 645 556 498 447 303 252 192 178 144 117 114 102 84 81 63 63 55 44 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4876 7709 9130 9504 9650 387 Size: 1064 138 3287 2833 1421 374 146 1280 Fragments arranged by size: 3287 2833 1421 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5647 6313 6481 7156 5647 Size: 666 168 675 9034 Fragments arranged by size: 9034 675 666 168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2393 2898 3039 3120 3548 3713 Size: 393 654 107 505 141 81 428 165 Cuts at: 3713 4287 4551 4616 4691 4697 4805 5135 5225 Size: 574 264 65 75 6 108 330 90 Cuts at: 5225 5336 5357 5510 6329 6870 6902 6905 6962 Size: 111 21 153 819 541 32 3 57 Cuts at: 6962 7061 7067 7250 7284 7367 7455 7538 7637 Size: 99 6 183 34 83 88 83 99 Cuts at: 7637 8606 9203 9990 10258 1239 Size: 969 597 787 268 1524 Fragments arranged by size: 1524 969 819 787 654 597 574 541 505 428 393 330 268 264 183 165 153 141 111 108 107 99 99 90 88 83 83 81 75 65 57 34 32 21 6 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 2348 4528 4682 5156 6041 6338 2348 Size: 2180 154 474 885 297 6553 Fragments arranged by size: 6553 2180 885 474 297 154 EcoO109I rG'GnC_Cy Cuts at: 2034 3375 3376 4224 6394 6901 6994 7107 2034 Size: 1341 1 848 2170 507 93 113 5470 Fragments arranged by size: 5470 2170 1341 848 507 113 93 1 EcoRI G'AATT_C Cuts at: 5311 7852 8230 5311 Size: 2541 378 7624 Fragments arranged by size: 7624 2541 378 EcoRV GAT'ATC Cuts at: 2836 5005 2836 Size: 2169 8374 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3857 4292 5725 5977 6770 3857 Size: 435 1433 252 793 7630 Fragments arranged by size: 7630 1433 793 435 252 FspI TGC'GCA Cuts at: 9049 9049 Size: 10543 HaeII r_GCGC'y Cuts at: 1678 2383 5806 9690 1678 Size: 705 3423 3884 2531 Fragments arranged by size: 3884 3423 2531 705 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2284 2316 2750 2782 4006 Size: 32 135 32 712 32 434 32 1224 Cuts at: 4006 4038 4302 4334 4367 4399 4537 4569 5996 Size: 32 264 32 33 32 138 32 1427 Cuts at: 5996 6028 6311 6343 6556 6588 7091 7123 7621 Size: 32 283 32 213 32 503 32 498 Cuts at: 7621 7653 9184 9216 9258 9290 1373 Size: 32 1531 32 42 32 2626 Fragments arranged by size: 2626 1531 1427 1224 712 503 498 434 283 264 213 138 135 42 33 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 7887 8133 669 Size: 988 281 5949 246 3079 Fragments arranged by size: 5949 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3594 6764 748 Size: 2846 3170 4527 Fragments arranged by size: 4527 3170 2846 HpaI GTT'AAC Cuts at: 8133 8133 Size: 10543 KpnI G_GTAC'C Cuts at: 3800 7868 3800 Size: 4068 6475 MfeI C'AATT_G Cuts at: 3508 8142 3508 Size: 4634 5909 MluI A'CGCG_T Cuts at: 10419 10419 Size: 10543 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3903 4788 9657 9841 1305 Size: 2598 885 4869 184 2007 Fragments arranged by size: 4869 2598 2007 885 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2388 3032 6260 10 Size: 54 1425 343 556 644 3228 4293 Fragments arranged by size: 4293 3228 1425 644 556 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2330 2346 2789 2799 4325 4508 6254 Size: 648 1164 16 443 10 1526 183 1746 Cuts at: 6254 8501 8860 9019 518 Size: 2247 359 159 2042 Fragments arranged by size: 2247 2042 1746 1526 1164 648 443 359 183 159 16 10 MspA1I CmG'CkG Cuts at: 1606 4265 4783 5521 5995 6301 6307 6379 7009 Size: 2659 518 738 474 306 6 72 630 Cuts at: 7009 7959 8636 9784 10029 1606 Size: 950 677 1148 245 2120 Fragments arranged by size: 2659 2120 1148 950 738 677 630 518 474 306 245 72 6 NcoI C'CATG_G Cuts at: 513 1065 2004 3045 6249 513 Size: 552 939 1041 3204 4807 Fragments arranged by size: 4807 3204 1041 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 10543 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10543 NotI GC'GGCC_GC Cuts at: 7990 7990 Size: 10543 NsiI A_TGCA'T Cuts at: 4058 7580 4058 Size: 3522 7021 NspI r_CATG'y Cuts at: 1132 1229 2554 2815 3520 5630 7901 9446 1132 Size: 97 1325 261 705 2110 2271 1545 2229 Fragments arranged by size: 2271 2229 2110 1545 1325 705 261 97 PasI CC'CwG_GG Cuts at: 1348 6967 1348 Size: 5619 4924 PciI A'CATG_T Cuts at: 3516 9442 3516 Size: 5926 4617 PflMI CCAn_nnn'nTGG Cuts at: 1108 5467 1108 Size: 4359 6184 PfoI T'CCnGG_A Cuts at: 2669 7158 2669 Size: 4489 6054 PmeI GTTT'AAAC Cuts at: 7847 7847 Size: 10543 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 8612 8644 10125 10157 8612 Size: 32 1481 32 8998 Fragments arranged by size: 8998 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4224 6901 6994 7107 4224 Size: 2677 93 113 7660 Fragments arranged by size: 7660 2677 113 93 PshAI GACnn'nnGTC Cuts at: 1613 6656 1613 Size: 5043 5500 PsiI TTA'TAA Cuts at: 3804 8113 3804 Size: 4309 6234 PspOMI G'GGCC_C Cuts at: 3375 3375 Size: 10543 PspXI vC'TCGA_Gb Cuts at: 5424 5648 5424 Size: 224 10319 PstI C_TGCA'G Cuts at: 830 4182 6042 6306 6355 6384 6834 7895 830 Size: 3352 1860 264 49 29 450 1061 3478 Fragments arranged by size: 3478 3352 1860 1061 450 264 49 29 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10543 PvuII CAG'CTG Cuts at: 4265 4783 5521 5995 6301 6307 6379 7009 4265 Size: 518 738 474 306 6 72 630 7799 Fragments arranged by size: 7799 738 630 518 474 306 72 6 SacI G_AGCT'C Cuts at: 5649 6315 6483 7158 5649 Size: 666 168 675 9034 Fragments arranged by size: 9034 675 666 168 SalI G'TCGA_C Cuts at: 1936 7885 1936 Size: 5949 4594 SapI GCTCTTCn'nnn_ Cuts at: 3144 4541 4740 5268 5793 7146 3144 Size: 1397 199 528 525 1353 6541 Fragments arranged by size: 6541 1397 1353 528 525 199 SbfI CC_TGCA'GG Cuts at: 7895 7895 Size: 10543 ScaI AGT'ACT Cuts at: 3919 8791 10503 3919 Size: 4872 1712 3959 Fragments arranged by size: 4872 3959 1712 SexAI A'CCwGG_T Cuts at: 4081 6582 4081 Size: 2501 8042 SfcI C'TryA_G Cuts at: 826 1045 2132 3288 3669 4109 4178 4950 5669 Size: 219 1087 1156 381 440 69 772 719 Cuts at: 5669 6038 6302 6351 6380 6830 7891 9026 9707 Size: 369 264 49 29 450 1061 1135 681 Cuts at: 9707 9898 826 Size: 191 1471 Fragments arranged by size: 1471 1156 1135 1087 1061 772 719 681 450 440 381 369 264 219 191 69 49 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10543 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10543 SmaI CCC'GGG Cuts at: 1872 7870 1872 Size: 5998 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3443 3458 5141 5153 5424 5648 Size: 197 926 1500 15 1683 12 271 224 Cuts at: 5648 6485 6491 7568 7785 8647 9548 9810 820 Size: 837 6 1077 217 862 901 262 1553 Fragments arranged by size: 1683 1553 1500 1077 926 901 862 837 271 262 224 217 197 15 12 6 SnaBI TAC'GTA Cuts at: 493 8334 493 Size: 7841 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 10543 SphI G_CATG'C Cuts at: 1132 2815 7901 1132 Size: 1683 5086 3774 Fragments arranged by size: 5086 3774 1683 SspI AAT'ATT Cuts at: 5 52 8467 5 Size: 47 8415 2081 Fragments arranged by size: 8415 2081 47 StuI AGG'CCT Cuts at: 1517 5337 1517 Size: 3820 6723 StyI C'CwwG_G Cuts at: 513 1065 2004 2080 2145 3045 4220 4284 4626 Size: 552 939 76 65 900 1175 64 342 Cuts at: 4626 5028 6249 6279 7982 513 Size: 402 1221 30 1703 3074 Fragments arranged by size: 3074 1703 1221 1175 939 900 552 402 342 76 65 64 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3553 5474 8570 8587 8740 8925 9370 Size: 1255 1973 1921 3096 17 153 185 445 Cuts at: 9370 325 Size: 1498 Fragments arranged by size: 3096 1973 1921 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5786 6473 Size: 274 80 33 51 1443 1940 1869 687 Cuts at: 6473 8789 10501 96 Size: 2316 1712 138 Fragments arranged by size: 2316 1940 1869 1712 1443 687 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2598 2630 2963 2995 4141 4173 5875 5907 6410 Size: 32 333 32 1146 32 1702 32 503 Cuts at: 6410 6442 10503 10535 2598 Size: 32 4061 32 2606 Fragments arranged by size: 4061 2606 1702 1146 503 333 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 10543 XbaI T'CTAG_A Cuts at: 7879 7879 Size: 10543 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3052 3886 4310 4780 6214 7372 3052 Size: 834 424 470 1434 1158 6223 Fragments arranged by size: 6223 1434 1158 834 470 424 XhoI C'TCGA_G Cuts at: 1943 5424 5648 1943 Size: 3481 224 6838 Fragments arranged by size: 6838 3481 224 XmaI C'CCGG_G Cuts at: 1870 7868 1870 Size: 5998 4545 XmnI GAAnn'nnTTC Cuts at: 4164 6766 8672 4164 Size: 2602 1906 6035 Fragments arranged by size: 6035 2602 1906 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8351 276 Size: 53 83 186 936 6817 2468 Fragments arranged by size: 6817 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbeI BsiWI BsmBI DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI