Restriction map for pFN21AA1000
 (Linear) (Six-base) MAPSORT of: KIAA1000.seq  Check: 9215  from: 1  to: 5838



KIAA1000.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    4929    5034    5838 
   Size:       4929     105     804

  Fragments arranged by size: 

                4929     804     105
 
AccI GT'mk_AC

Cuts at:      0    2082    5838 
   Size:       2082    3756
 
Acc65I G'GTAC_C

Cuts at:      0    1791    5838 
   Size:       1791    4047
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     388    1034    2282    2546    2611    2686    2692    3130 
   Size:        388     646    1248     264      65      75       6     438

Cuts at:   3130    3331    3352    4865    4897    4957    5056    5062    5279 
   Size:        201      21    1513      32      60      99       6     217

Cuts at:   5279    5362    5450    5632    5838 
   Size:         83      88     182     206

  Fragments arranged by size: 

                1513    1248     646     438     388     264     217     206
                 201     182      99      88      83      75      65      60
                  32      21       6       6
 
AfeI AGC'GCT

Cuts at:      0    3799    5838 
   Size:       3799    2039
 
AflIII A'CryG_T

Cuts at:      0    1511    5838 
   Size:       1511    4327
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4320    5838 
   Size:       4320    1518
 
AleI CACnn'nnGTG

Cuts at:      0     794    2320    5838 
   Size:        794    1526    3518

  Fragments arranged by size: 

                3518    1526     794
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     812    2440    5838 
   Size:        812    1628    3398

  Fragments arranged by size: 

                3398    1628     812
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1700    1732    5838 
   Size:       1700      32    4106

  Fragments arranged by size: 

                4106    1700      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     991    3054    3555    3684    5838 
   Size:        991    2063     501     129    2154

  Fragments arranged by size: 

                2154    2063     991     501     129
 
ApaI G_GGCC'C

Cuts at:      0    1374    5838 
   Size:       1374    4464
 
ApaLI G'TGCA_C

Cuts at:      0    2603    5838 
   Size:       2603    3235
 
ApoI r'AATT_y

Cuts at:      0     775     902    1123    1750    2434    2832    3306    3544 
   Size:        775     127     221     627     684     398     474     238

Cuts at:   3544    4788    5838 
   Size:       1244    1050

  Fragments arranged by size: 

                1244    1050     775     684     627     474     398     238
                 221     127
 
AseI AT'TA_AT

Cuts at:      0    1469    5838 
   Size:       1469    4369
 
AvaI C'yCGr_G

Cuts at:      0    3419    3643    4309    4330    5838 
   Size:       3419     224     666      21    1508

  Fragments arranged by size: 

                3419    1508     666     224      21
 
BamHI G'GATC_C

Cuts at:      0    3499    5838 
   Size:       3499    2339
 
BanI G'GyrC_C

Cuts at:      0     796    1309    1791    3142    4289    5838 
   Size:        796     513     482    1351    1147    1549

  Fragments arranged by size: 

                1549    1351    1147     796     513     482
 
BanII G_rGCy'C

Cuts at:      0    1374    3644    4310    4478    4686    5153    5556    5838 
   Size:       1374    2270     666     168     208     467     403     282

  Fragments arranged by size: 

                2270    1374     666     467     403     282     208     168
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     323    1862    2379    3358    3980    4193    4651    5609 
   Size:        323    1539     517     979     622     213     458     958

Cuts at:   5609    5838 
   Size:        229

  Fragments arranged by size: 

                1539     979     958     622     517     458     323     229
                 213
 
BbvCI CC'TCA_GC

Cuts at:      0     197    3274    5206    5838 
   Size:        197    3077    1932     632

  Fragments arranged by size: 

                3077    1932     632     197
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2679    5838 
   Size:       2679    3159
 
BfrBI ATG'CAT

Cuts at:      0    2051    5573    5838 
   Size:       2051    3522     265

  Fragments arranged by size: 

                3522    2051     265
 
BglI GCCn_nnn'nGGC

Cuts at:      0    5305    5317    5838 
   Size:       5305      12     521

  Fragments arranged by size: 

                5305     521      12
 
BglII A'GATC_T

Cuts at:      0    1599    5838 
   Size:       1599    4239
 
BlpI GC'TnA_GC

Cuts at:      0     113    2714    3085    3341    3512    3638    4043    4100 
   Size:        113    2601     371     256     171     126     405      57

Cuts at:   4100    4921    5057    5838 
   Size:        821     136     781

  Fragments arranged by size: 

                2601     821     781     405     371     256     171     136
                 126     113      57
 
Bme1580I G_kGCm'C

Cuts at:      0    1314    1374    2607    3147    4020    5838 
   Size:       1314      60    1233     540     873    1818

  Fragments arranged by size: 

                1818    1314    1233     873     540      60
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2292    5838 
   Size:       2292    3546
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3466    5838 
   Size:       3466    2372
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     893    1115    1543    1708    2800    3220    3505 
   Size:        281     612     222     428     165    1092     420     285

Cuts at:   3505    4324    4900    5245    5533    5838 
   Size:        819     576     345     288     305

  Fragments arranged by size: 

                1092     819     612     576     428     420     345     305
                 288     285     281     222     165
 
Bpu10I CC'TnA_GC

Cuts at:      0     197    3094    3274    3758    5206    5838 
   Size:        197    2897     180     484    1448     632

  Fragments arranged by size: 

                2897    1448     632     484     197     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1459    1474    3157    3169    4465    4471    5584    5765 
   Size:       1459      15    1683      12    1296       6    1113     181

Cuts at:   5765    5838 
   Size:         73

  Fragments arranged by size: 

                1683    1459    1296    1113     181      73      15      12
                   6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     415    3994    5838 
   Size:        415    3579    1844

  Fragments arranged by size: 

                3579    1844     415
 
BsaBI GATnn'nnATC

Cuts at:      0    2428    4981    5838 
   Size:       2428    2553     857

  Fragments arranged by size: 

                2553    2428     857
 
BsaHI Gr'CG_yC

Cuts at:      0    4316    5838 
   Size:       4316    1522
 
BsaWI w'CCGG_w

Cuts at:      0    4109    5838 
   Size:       4109    1729
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     958     988    2564    2594    3870    3900    5838 
   Size:        958      30    1576      30    1276      30    1938

  Fragments arranged by size: 

                1938    1576    1276     958      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     750    1200    2839    3088    3091    3502    4432    4468 
   Size:        750     450    1639     249       3     411     930      36

Cuts at:   4468    4516    5194    5838 
   Size:         48     678     644

  Fragments arranged by size: 

                1639     930     750     678     644     450     411     249
                  48      36       3
 
BseYI C'CCAG_C

Cuts at:      0    2335    5838 
   Size:       2335    3503
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    3049    3052    3175    3541    3568    4090    5290    5555 
   Size:       3049       3     123     366      27     522    1200     265

Cuts at:   5555    5838 
   Size:        283

  Fragments arranged by size: 

                3049    1200     522     366     283     265     123      27
                   3
 
BsiHKAI G_wGCw'C

Cuts at:      0    2412    2607    2954    3644    4310    4478    5153    5838 
   Size:       2412     195     347     690     666     168     675     685

  Fragments arranged by size: 

                2412     690     685     675     666     347     195     168
            
 
BsmI GAATG_Cn'

Cuts at:      0     186    1409    2204    5838 
   Size:        186    1223     795    3634

  Fragments arranged by size: 

                3634    1223     795     186
 
Bsp1286I G_dGCh'C

Cuts at:      0    1314    1374    2412    2607    2954    3147    3644    4020 
   Size:       1314      60    1038     195     347     193     497     376

Cuts at:   4020    4310    4478    4686    5153    5556    5838 
   Size:        290     168     208     467     403     282

  Fragments arranged by size: 

                1314    1038     497     467     403     376     347     290
                 282     208     195     193     168      60
 
BspHI T'CATG_A

Cuts at:      0     927    5838 
   Size:        927    4911
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     941    3071    3660    4929    5034    5838 
   Size:        941    2130     589    1269     105     804

  Fragments arranged by size: 

                2130    1269     941     804     589     105
 
BsrBI CCG'CTC

Cuts at:      0    4343    5061    5838 
   Size:       4343     718     777

  Fragments arranged by size: 

                4343     777     718
 
BsrDI GCAATG_nn'

Cuts at:      0     332    4150    5360    5838 
   Size:        332    3818    1210     478

  Fragments arranged by size: 

                3818    1210     478     332
 
BsrFI r'CCGG_y

Cuts at:      0    4934    5838 
   Size:       4934     904
 
BssSI C'ACGA_G

Cuts at:      0    4414    5838 
   Size:       4414    1424
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    4296    5838 
   Size:       4296    1542
 
BstEII G'GTnAC_C

Cuts at:      0    1293    4866    5838 
   Size:       1293    3573     972

  Fragments arranged by size: 

                3573    1293     972
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     141     871    4251    4962    5838 
   Size:        141     730    3380     711     876

  Fragments arranged by size: 

                3380     876     730     711     141
 
BstYI r'GATC_y

Cuts at:      0      63    1599    2771    3499    5094    5838 
   Size:         63    1536    1172     728    1595     744

  Fragments arranged by size: 

                1595    1536    1172     744     728      63
 
BstZ17I GTA'TAC

Cuts at:      0    2083    5838 
   Size:       2083    3755
 
Bsu36I CC'TnA_GG

Cuts at:      0    1658    5838 
   Size:       1658    4180
 
BtgI C'CryG_G

Cuts at:      0    1040    4244    5838 
   Size:       1040    3204    1594

  Fragments arranged by size: 

                3204    1594    1040
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    5583    5838 
   Size:       5583     255
 
BtsI GCAGTG_nn'

Cuts at:      0    2752    4813    5538    5838 
   Size:       2752    2061     725     300

  Fragments arranged by size: 

                2752    2061     725     300
 
ClaI AT'CG_AT

Cuts at:      0    2897    5838 
   Size:       2897    2941
 
DraI TTT'AAA

Cuts at:      0    1128    2190    2331    3212    5838 
   Size:       1128    1062     141     881    2626

  Fragments arranged by size: 

                2626    1128    1062     881     141
 
EaeI y'GGCC_r

Cuts at:      0     381     470    1025    4253    5127    5838 
   Size:        381      89     555    3228     874     711

  Fragments arranged by size: 

                3228     874     711     555     381      89
 
EarI CTCTTCn'nnn_

Cuts at:      0     418     481     583    1139    1637    2536    2591    2735 
   Size:        418      63     102     556     498     899      55     144

Cuts at:   2735    2816    3263    3566    3610    3788    3905    4550    4634 
   Size:         81     447     303      44     178     117     645      84

Cuts at:   4634    4886    5078    5141    5255    5285    5838 
   Size:        252     192      63     114      30     553

  Fragments arranged by size: 

                 899     645     556     553     498     447     418     303
                 252     192     178     144     117     114     102      84
                  81      63      63      55      44      30
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2871    5704    5838 
   Size:       2871    2833     134

  Fragments arranged by size: 

                2871    2833     134
 
EcoICRI GAG'CTC

Cuts at:      0    3642    4308    4476    5151    5838 
   Size:       3642     666     168     675     687

  Fragments arranged by size: 

                3642     687     675     666     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     388     893    1034    1115    1543    1708    2282 
   Size:        281     107     505     141      81     428     165     574

Cuts at:   2282    2546    2611    2686    2692    2800    3130    3220    3331 
   Size:        264      65      75       6     108     330      90     111

Cuts at:   3331    3352    3505    4324    4865    4897    4900    4957    5056 
   Size:         21     153     819     541      32       3      57      99

Cuts at:   5056    5062    5245    5279    5362    5450    5533    5632    5838 
   Size:          6     183      34      83      88      83      99     206

  Fragments arranged by size: 

                 819     574     541     505     428     330     281     264
                 206     183     165     153     141     111     108     107
                  99      99      90      88      83      83      81      75
                  65      57      34      32      21       6       6       3
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     343    2523    2677    3151    4036    4333    5838 
   Size:        343    2180     154     474     885     297    1505

  Fragments arranged by size: 

                2180    1505     885     474     343     297     154
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29    1370    1371    2219    4389    4896    4989    5102 
   Size:         29    1341       1     848    2170     507      93     113

Cuts at:   5102    5838 
   Size:        736

  Fragments arranged by size: 

                2170    1341     848     736     507     113      93      29
                   1
 
EcoRI G'AATT_C

Cuts at:      0    3306    5838 
   Size:       3306    2532
 
EcoRV GAT'ATC

Cuts at:      0     831    3000    5838 
   Size:        831    2169    2838

  Fragments arranged by size: 

                2838    2169     831
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1852    2287    3720    3972    4765    5838 
   Size:       1852     435    1433     252     793    1073

  Fragments arranged by size: 

                1852    1433    1073     793     435     252
 
HaeII r_GCGC'y

Cuts at:      0     378    3801    5838 
   Size:        378    3423    2037

  Fragments arranged by size: 

                3423    2037     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     279     311     745     777    2001    2033    2297    2329 
   Size:        279      32     434      32    1224      32     264      32

Cuts at:   2329    2362    2394    2532    2564    3991    4023    4306    4338 
   Size:         33      32     138      32    1427      32     283      32

Cuts at:   4338    4551    4583    5086    5118    5616    5648    5838 
   Size:        213      32     503      32     498      32     190

  Fragments arranged by size: 

                1427    1224     503     498     434     283     279     264
                 213     190     138      33      32      32      32      32
                  32      32      32      32      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1589    4759    5838 
   Size:       1589    3170    1079

  Fragments arranged by size: 

                3170    1589    1079
 
KpnI G_GTAC'C

Cuts at:      0    1795    5838 
   Size:       1795    4043
 
MfeI C'AATT_G

Cuts at:      0    1503    5838 
   Size:       1503    4335
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1898    2783    5838 
   Size:       1898     885    3055

  Fragments arranged by size: 

                3055    1898     885
 
MscI TGG'CCA

Cuts at:      0     383    1027    4255    5838 
   Size:        383     644    3228    1583

  Fragments arranged by size: 

                3228    1583     644     383
 
MslI CAynn'nnrTG

Cuts at:      0     325     341     784     794    2320    2503    4249    5838 
   Size:        325      16     443      10    1526     183    1746    1589

  Fragments arranged by size: 

                1746    1589    1526     443     325     183      16      10
            
 
MspA1I CmG'CkG

Cuts at:      0    2260    2778    3516    3990    4296    4302    4374    5004 
   Size:       2260     518     738     474     306       6      72     630

Cuts at:   5004    5838 
   Size:        834

  Fragments arranged by size: 

                2260     834     738     630     518     474     306      72
                   6
 
NcoI C'CATG_G

Cuts at:      0    1040    4244    5838 
   Size:       1040    3204    1594

  Fragments arranged by size: 

                3204    1594    1040
 
NsiI A_TGCA'T

Cuts at:      0    2053    5575    5838 
   Size:       2053    3522     263

  Fragments arranged by size: 

                3522    2053     263
 
NspI r_CATG'y

Cuts at:      0     549     810    1515    3625    5838 
   Size:        549     261     705    2110    2213

  Fragments arranged by size: 

                2213    2110     705     549     261
 
PasI CC'CwG_GG

Cuts at:      0    4962    5838 
   Size:       4962     876
 
PciI A'CATG_T

Cuts at:      0    1511    5838 
   Size:       1511    4327
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    3462    5838 
   Size:       3462    2376
 
PfoI T'CCnGG_A

Cuts at:      0     664    5153    5838 
   Size:        664    4489     685

  Fragments arranged by size: 

                4489     685     664
 
PpuMI rG'GwC_Cy

Cuts at:      0    2219    4896    4989    5102    5838 
   Size:       2219    2677      93     113     736

  Fragments arranged by size: 

                2677    2219     736     113      93
 
PshAI GACnn'nnGTC

Cuts at:      0    4651    5838 
   Size:       4651    1187
 
PsiI TTA'TAA

Cuts at:      0    1799    5838 
   Size:       1799    4039
 
PspOMI G'GGCC_C

Cuts at:      0    1370    5838 
   Size:       1370    4468
 
PspXI vC'TCGA_Gb

Cuts at:      0    3419    3643    5838 
   Size:       3419     224    2195

  Fragments arranged by size: 

                3419    2195     224
 
PstI C_TGCA'G

Cuts at:      0    2177    4037    4301    4350    4379    4829    5838 
   Size:       2177    1860     264      49      29     450    1009

  Fragments arranged by size: 

                2177    1860    1009     450     264      49      29
 
PvuII CAG'CTG

Cuts at:      0    2260    2778    3516    3990    4296    4302    4374    5004 
   Size:       2260     518     738     474     306       6      72     630

Cuts at:   5004    5838 
   Size:        834

  Fragments arranged by size: 

                2260     834     738     630     518     474     306      72
                   6
 
SacI G_AGCT'C

Cuts at:      0    3644    4310    4478    5153    5838 
   Size:       3644     666     168     675     685

  Fragments arranged by size: 

                3644     685     675     666     168
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1139    2536    2735    3263    3788    5141    5838 
   Size:       1139    1397     199     528     525    1353     697

  Fragments arranged by size: 

                1397    1353    1139     697     528     525     199
 
ScaI AGT'ACT

Cuts at:      0    1914    5838 
   Size:       1914    3924
 
SexAI A'CCwGG_T

Cuts at:      0    2076    4577    5838 
   Size:       2076    2501    1261

  Fragments arranged by size: 

                2501    2076    1261
 
SfcI C'TryA_G

Cuts at:      0     127    1283    1664    2104    2173    2945    3664    4033 
   Size:        127    1156     381     440      69     772     719     369

Cuts at:   4033    4297    4346    4375    4825    5838 
   Size:        264      49      29     450    1013

  Fragments arranged by size: 

                1156    1013     772     719     450     440     381     369
                 264     127      69      49      29
 
SmlI C'TyrA_G

Cuts at:      0    1438    1453    3136    3148    3419    3643    4480    4486 
   Size:       1438      15    1683      12     271     224     837       6

Cuts at:   4486    5563    5780    5838 
   Size:       1077     217      58

  Fragments arranged by size: 

                1683    1438    1077     837     271     224     217      58
                  15      12       6
 
SphI G_CATG'C

Cuts at:      0     810    5838 
   Size:        810    5028
 
StuI AGG'CCT

Cuts at:      0    3332    5838 
   Size:       3332    2506
 
StyI C'CwwG_G

Cuts at:      0      75     140    1040    2215    2279    2621    3023    4244 
   Size:         75      65     900    1175      64     342     402    1221

Cuts at:   4244    4274    5838 
   Size:         30    1564

  Fragments arranged by size: 

                1564    1221    1175     900     402     342      75      65
                  64      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1548    3469    5838 
   Size:       1548    1921    2369

  Fragments arranged by size: 

                2369    1921    1548
 
TatI w'GTAC_w

Cuts at:      0    1912    3781    4468    5838 
   Size:       1912    1869     687    1370

  Fragments arranged by size: 

                1912    1869    1370     687
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     593     625     958     990    2136    2168    3870    3902 
   Size:        593      32     333      32    1146      32    1702      32

Cuts at:   3902    4405    4437    5838 
   Size:        503      32    1401

  Fragments arranged by size: 

                1702    1401    1146     593     503     333      32      32
                  32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1047    1881    2305    2775    4209    5367    5838 
   Size:       1047     834     424     470    1434    1158     471

  Fragments arranged by size: 

                1434    1158    1047     834     471     470     424
 
XhoI C'TCGA_G

Cuts at:      0    3419    3643    5838 
   Size:       3419     224    2195

  Fragments arranged by size: 

                3419    2195     224
 
XmnI GAAnn'nnTTC

Cuts at:      0    2159    4761    5838 
   Size:       2159    2602    1077

  Fragments arranged by size: 

                2602    2159    1077
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI
    BamHI     BanI    BanII     BbsI    BbvCI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI     DraI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI    HaeII    Hin4I
  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I     NcoI
     NsiI     NspI     PasI     PciI    PflMI     PfoI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PstI    PvuII     SacI     SapI     ScaI
    SexAI     SfcI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AscI    AsiSI    AvrII     BaeI
     BbeI     BcgI     BclI    BmgBI     BmtI    BsaAI    BsiEI    BsiWI
    BsmBI    BspEI    BsrGI   BssHII    BstBI    CspCI   DraIII     DrdI
     EagI     FseI     FspI    FspAI   HincII     HpaI     KasI     MluI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PmeI     PmlI     PpiI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     SwaI  Tth111I     XbaI     XmaI     ZraI
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