Restriction map for
pFN21AA0868
##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI BamHI BbrPI
BbvCI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsiEI BsiWI BsmBI
Bsp1407I BsrGI Bst1107I BstXI BstZ17I
CelII CpoI DraI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FbaI
FseI HpaI KpnI KspI MfeI
MluI MunI NheI NotI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 651 GACGTC GACGT^C
AccIII 2881 TCCGGA T^CCGGA
Alw44I 3828 GTGCAC G^TGCAC
AocI 1343 CCTCAGG CC^T[ACGT]AGG
ApaLI 3828 GTGCAC G^TGCAC
AviII 476 TGCGCA TGC^GCA
AvrII 1812 CCTAGG C^CTAGG
BbeI 3177 GGCGCC GGCGC^C
BcgI 1374 GCACGAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1750 GGATAC GTATCC(6/5)
BlnI 1812 CCTAGG C^CTAGG
BsaBI 3385 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2881 TCCGGA T^CCGGA
BspEI 2881 TCCGGA T^CCGGA
BsrBI 2811 CCGCTC CCGCTC(-3/-3)
BssHII 15 GCGCGC G^CGCGC
BstAPI 3067 GCAACAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1445 TTCGAA TT^CGAA
Bsu36I 1343 CCTCAGG CC^T[ACGT]AGG
DraIII 2112 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 3433 CTCTTC CTCTTC(1/4)
Eco81I 1343 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1557 ATGCAT ATGCA^T
FspI 476 TGCGCA TGC^GCA
HindIII 3249 AAGCTT A^AGCTT
KasI 3177 GGCGCC G^GCGCC
Ksp632I 3433 CTCTTC CTCTTC(1/4)
MamI 3385 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2881 TCCGGA T^CCGGA
NaeI 19 GCCGGC GCC^GGC
NarI 3177 GGCGCC GG^CGCC
NcoI 2003 CCATGG C^CATGG
NdeI 2984 CATATG CA^TATG
NgoMIV 19 GCCGGC G^CCGGC
NruI 1631 TCGCGA TCG^CGA
NsiI 1557 ATGCAT ATGCA^T
NspV 1445 TTCGAA TT^CGAA
PflMI 2304 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 57 CAGCTG CAG^CTG
SacI 2749 GAGCTC GAGCT^C
SfoI 3177 GGCGCC GGC^GCC
SfuI 1445 TTCGAA TT^CGAA
SspI 3295 AATATT AAT^ATT
Van91I 2304 CCAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 3097 GTAGAC GT^[AC][GT]AC
3304 GTAGAC
AflIII 1649 ACATGT A^C[GA][CT]GT
3854 ACATGT
Aor51HI 33 AGCGCT AGC^GCT
2458 AGCGCT
AvaI 2889 CTCGGG C^[CT]CG[GA]G
3281 CCCGAG
BanII 1069 GAGCCC G[GA]GC[CT]^C
2749 GAGCTC
BsoBI 2889 CTCGGG C^[CT]CG[GA]G
3281 CCCGAG
BspDI 1333 ATCGAT AT^CGAT
1420 ATCGAT
BspHI 1923 TCATGA T^CATGA
3932 TCATGA
BspLU11I 1649 ACATGT A^CATGT
3854 ACATGT
BsrFI 19 GCCGGC [GA]^CCGG[CT]
2696 ACCGGC
BssSI 1375 CACGAG CACGAG(-5/-1)
3358 CACGAG
BstEII 327 GGTGACC G^GT[ACGT]ACC
2615 GGTCACC
BstPI 327 GGTGACC G^GT[ACGT]ACC
2615 GGTCACC
Cfr10I 19 GCCGGC [GA]^CCGG[CT]
2696 ACCGGC
ClaI 1333 ATCGAT AT^CGAT
1420 ATCGAT
DrdI 427 GACGGTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1911 GACCATAGTGTC
DsaI 2003 CCATGG C^C[GA][CT]GG
2952 CCACGG
Eco47III 33 AGCGCT AGC^GCT
2458 AGCGCT
EcoNI 1389 CCTAGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3135 CCTGGCACAGG
EcoO65I 327 GGTGACC G^GT[ACGT]ACC
2615 GGTCACC
PpuMI 1175 AGGACCT [GA]G^G[AT]CC[CT]
2358 AGGTCCT
PstI 66 CTGCAG CTGCA^G
2657 CTGCAG
RcaI 1923 TCATGA T^CATGA
3932 TCATGA
SphI 884 GCATGC GCATG^C
1559 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII