Restriction map for
pFN21AA0868
(Linear) (Six-base) MAPSORT of: KIAA0868.seq Check: 5373 from: 1 to: 3993
KIAA0868.orf
With 182 enzymes: SgfI *
March 3, 2008 10:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1774 2300 3993
Size: 1774 526 1693
Fragments arranged by size:
1774 1693 526
AatII G_ACGT'C
Cuts at: 0 655 3993
Size: 655 3338
AccI GT'mk_AC
Cuts at: 0 3098 3305 3993
Size: 3098 207 688
Fragments arranged by size:
3098 688 207
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1218 1522 2569 3141 3147 3993
Size: 238 980 304 1047 572 6 846
Fragments arranged by size:
1047 980 846 572 304 238 6
AfeI AGC'GCT
Cuts at: 0 35 2460 3993
Size: 35 2425 1533
Fragments arranged by size:
2425 1533 35
AflIII A'CryG_T
Cuts at: 0 1649 3854 3993
Size: 1649 2205 139
Fragments arranged by size:
2205 1649 139
AleI CACnn'nnGTG
Cuts at: 0 790 2563 3993
Size: 790 1773 1430
Fragments arranged by size:
1773 1430 790
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 302 3993
Size: 302 3691
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1440 1472 3993
Size: 1440 32 2521
Fragments arranged by size:
2521 1440 32
AlwNI CAG_nnn'CTG
Cuts at: 0 59 74 1179 1351 3993
Size: 59 15 1105 172 2642
Fragments arranged by size:
2642 1105 172 59 15
ApaLI G'TGCA_C
Cuts at: 0 3828 3993
Size: 3828 165
ApoI r'AATT_y
Cuts at: 0 993 1086 1401 1593 3993
Size: 993 93 315 192 2400
Fragments arranged by size:
2400 993 315 192 93
AvaI C'yCGr_G
Cuts at: 0 2889 3281 3993
Size: 2889 392 712
Fragments arranged by size:
2889 712 392
AvrII C'CTAG_G
Cuts at: 0 1812 3993
Size: 1812 2181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 882 915 2940 2973 3993
Size: 882 33 2025 33 1020
Fragments arranged by size:
2025 1020 882 33 33
BanI G'GyrC_C
Cuts at: 0 1119 1155 2645 3060 3177 3277 3692 3871
Size: 1119 36 1490 415 117 100 415 179
Cuts at: 3871 3993
Size: 122
Fragments arranged by size:
1490 1119 415 415 179 122 117 100
36
BanII G_rGCy'C
Cuts at: 0 1073 2753 3993
Size: 1073 1680 1240
Fragments arranged by size:
1680 1240 1073
BbeI G_GCGC'C
Cuts at: 0 3181 3993
Size: 3181 812
BbsI GAAGACnn'nnnn_
Cuts at: 0 1224 3370 3831 3993
Size: 1224 2146 461 162
Fragments arranged by size:
2146 1224 461 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1363 1397 3993
Size: 1363 34 2596
Fragments arranged by size:
2596 1363 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1744 3993
Size: 1744 2249
BfrBI ATG'CAT
Cuts at: 0 1559 3993
Size: 1559 2434
BglI GCCn_nnn'nGGC
Cuts at: 0 79 1164 2792 3869 3993
Size: 79 1085 1628 1077 124
Fragments arranged by size:
1628 1085 1077 124 79
Bme1580I G_kGCm'C
Cuts at: 0 1160 1679 3832 3874 3993
Size: 1160 519 2153 42 119
Fragments arranged by size:
2153 1160 519 119 42
BmgBI CAC'GTC
Cuts at: 0 92 3606 3993
Size: 92 3514 387
Fragments arranged by size:
3514 387 92
BmrI ACTGGGnnnn_n'
Cuts at: 0 558 2160 3993
Size: 558 1602 1833
Fragments arranged by size:
1833 1602 558
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1171 2563 2767 2866 3069 3522 3993
Size: 963 208 1392 204 99 203 453 471
Fragments arranged by size:
1392 963 471 453 208 204 203 99
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1506 2838 3361 3553 3600 3993
Size: 1506 1332 523 192 47 393
Fragments arranged by size:
1506 1332 523 393 192 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 227 1228 3952 3993
Size: 227 1001 2724 41
Fragments arranged by size:
2724 1001 227 41
BsaBI GATnn'nnATC
Cuts at: 0 3389 3993
Size: 3389 604
BsaHI Gr'CG_yC
Cuts at: 0 652 1031 1942 3178 3797 3923 3993
Size: 652 379 911 1236 619 126 70
Fragments arranged by size:
1236 911 652 619 379 126 70
BsaWI w'CCGG_w
Cuts at: 0 338 2881 3848 3993
Size: 338 2543 967 145
Fragments arranged by size:
2543 967 338 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1984 2014 2404 2434 2536 2566 2944 2974
Size: 1984 30 390 30 102 30 378 30
Cuts at: 2974 3187 3217 3993
Size: 213 30 776
Fragments arranged by size:
1984 776 390 378 213 102 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 141 144 218 3160 3181 3993
Size: 141 3 74 2942 21 812
Fragments arranged by size:
2942 812 141 74 21 3
BseYI C'CCAG_C
Cuts at: 0 211 973 2779 3993
Size: 211 762 1806 1214
Fragments arranged by size:
1806 1214 762 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 2190 2413 3993
Size: 661 1529 223 1580
Fragments arranged by size:
1580 1529 661 223
BsiHKAI G_wGCw'C
Cuts at: 0 1708 2753 3832 3993
Size: 1708 1045 1079 161
Fragments arranged by size:
1708 1079 1045 161
BsmI GAATG_Cn'
Cuts at: 0 828 2400 3185 3993
Size: 828 1572 785 808
Fragments arranged by size:
1572 828 808 785
Bsp1286I G_dGCh'C
Cuts at: 0 1073 1160 1679 1708 2753 3832 3874 3993
Size: 1073 87 519 29 1045 1079 42 119
Fragments arranged by size:
1079 1073 1045 519 119 87 42 29
BspEI T'CCGG_A
Cuts at: 0 2881 3993
Size: 2881 1112
BspHI T'CATG_A
Cuts at: 0 1923 3932 3993
Size: 1923 2009 61
Fragments arranged by size:
2009 1923 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1774 2300 2435 3993
Size: 1774 526 135 1558
Fragments arranged by size:
1774 1558 526 135
BsrBI CCG'CTC
Cuts at: 0 2813 3993
Size: 2813 1180
BsrDI GCAATG_nn'
Cuts at: 0 290 632 1266 2257 2895 3800 3993
Size: 290 342 634 991 638 905 193
Fragments arranged by size:
991 905 638 634 342 290 193
BsrFI r'CCGG_y
Cuts at: 0 19 2696 3993
Size: 19 2677 1297
Fragments arranged by size:
2677 1297 19
BssHII G'CGCG_C
Cuts at: 0 15 3993
Size: 15 3978
BssSI C'ACGA_G
Cuts at: 0 1375 3358 3993
Size: 1375 1983 635
Fragments arranged by size:
1983 1375 635
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3073 3993
Size: 3073 920
BstBI TT'CG_AA
Cuts at: 0 1446 3993
Size: 1446 2547
BstEII G'GTnAC_C
Cuts at: 0 327 2615 3993
Size: 327 2288 1378
Fragments arranged by size:
2288 1378 327
BstYI r'GATC_y
Cuts at: 0 1842 2211 2711 3149 3993
Size: 1842 369 500 438 844
Fragments arranged by size:
1842 844 500 438 369
Bsu36I CC'TnA_GG
Cuts at: 0 1344 3993
Size: 1344 2649
BtgI C'CryG_G
Cuts at: 0 2003 2952 3993
Size: 2003 949 1041
Fragments arranged by size:
2003 1041 949
BtsI GCAGTG_nn'
Cuts at: 0 2654 3993
Size: 2654 1339
ClaI AT'CG_AT
Cuts at: 0 1334 1421 3993
Size: 1334 87 2572
Fragments arranged by size:
2572 1334 87
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3255 3290 3993
Size: 3255 35 703
Fragments arranged by size:
3255 703 35
DraIII CAC_nnn'GTG
Cuts at: 0 2117 3993
Size: 2117 1876
DrdI GACnn_nn'nnGTC
Cuts at: 0 433 1917 3993
Size: 433 1484 2076
Fragments arranged by size:
2076 1484 433
EaeI y'GGCC_r
Cuts at: 0 573 780 3320 3993
Size: 573 207 2540 673
Fragments arranged by size:
2540 673 573 207
EarI CTCTTCn'nnn_
Cuts at: 0 3439 3993
Size: 3439 554
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 870 3666 3850 3916 3993
Size: 870 2796 184 66 77
Fragments arranged by size:
2796 870 184 77 66
EcoICRI GAG'CTC
Cuts at: 0 2751 3993
Size: 2751 1242
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 963 1171 1218 1522 2563 2569 2767
Size: 238 725 208 47 304 1041 6 198
Cuts at: 2767 2866 3069 3141 3147 3522 3993
Size: 99 203 72 6 375 471
Fragments arranged by size:
1041 725 471 375 304 238 208 203
198 99 72 47 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1393 3139 3993
Size: 1393 1746 854
Fragments arranged by size:
1746 1393 854
EcoO109I rG'GnC_Cy
Cuts at: 0 1176 1867 2359 2721 3644 3993
Size: 1176 691 492 362 923 349
Fragments arranged by size:
1176 923 691 492 362 349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3387 3471 3594 3993
Size: 3387 84 123 399
Fragments arranged by size:
3387 399 123 84
FspI TGC'GCA
Cuts at: 0 478 3993
Size: 478 3515
FspAI rTGC'GCAy
Cuts at: 0 478 3993
Size: 478 3515
HaeII r_GCGC'y
Cuts at: 0 37 849 2000 2462 3181 3993
Size: 37 812 1151 462 719 812
Fragments arranged by size:
1151 812 812 719 462 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 1327 1359 1420 1452 2343 2375
Size: 1168 32 127 32 61 32 891 32
Cuts at: 2375 2536 2568 2846 2878 3393 3425 3549 3581
Size: 161 32 278 32 515 32 124 32
Cuts at: 3581 3993
Size: 412
Fragments arranged by size:
1168 891 515 412 278 161 127 124
61 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 261 1035 3345 3771 3993
Size: 261 774 2310 426 222
Fragments arranged by size:
2310 774 426 261 222
HindIII A'AGCT_T
Cuts at: 0 3249 3993
Size: 3249 744
KasI G'GCGC_C
Cuts at: 0 3177 3993
Size: 3177 816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2295 3449 3659 3993
Size: 2295 1154 210 334
Fragments arranged by size:
2295 1154 334 210
MscI TGG'CCA
Cuts at: 0 575 782 3322 3993
Size: 575 207 2540 671
Fragments arranged by size:
2540 671 575 207
MslI CAynn'nnrTG
Cuts at: 0 386 790 1573 1648 2563 3805 3993
Size: 386 404 783 75 915 1242 188
Fragments arranged by size:
1242 915 783 404 386 188 75
MspA1I CmG'CkG
Cuts at: 0 59 1856 3654 3993
Size: 59 1797 1798 339
Fragments arranged by size:
1798 1797 339 59
NaeI GCC'GGC
Cuts at: 0 21 3993
Size: 21 3972
NarI GG'CG_CC
Cuts at: 0 3178 3993
Size: 3178 815
NcoI C'CATG_G
Cuts at: 0 2003 3993
Size: 2003 1990
NdeI CA'TA_TG
Cuts at: 0 2985 3993
Size: 2985 1008
NgoMIV G'CCGG_C
Cuts at: 0 19 3993
Size: 19 3974
NruI TCG'CGA
Cuts at: 0 1633 3993
Size: 1633 2360
NsiI A_TGCA'T
Cuts at: 0 1561 3993
Size: 1561 2432
NspI r_CATG'y
Cuts at: 0 888 1563 1653 3858 3993
Size: 888 675 90 2205 135
Fragments arranged by size:
2205 888 675 135 90
PasI CC'CwG_GG
Cuts at: 0 2495 3993
Size: 2495 1498
PciI A'CATG_T
Cuts at: 0 1649 3854 3993
Size: 1649 2205 139
Fragments arranged by size:
2205 1649 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 2310 3993
Size: 2310 1683
PfoI T'CCnGG_A
Cuts at: 0 408 3738 3993
Size: 408 3330 255
Fragments arranged by size:
3330 408 255
PpuMI rG'GwC_Cy
Cuts at: 0 1176 2359 3993
Size: 1176 1183 1634
Fragments arranged by size:
1634 1183 1176
PstI C_TGCA'G
Cuts at: 0 70 2661 3993
Size: 70 2591 1332
Fragments arranged by size:
2591 1332 70
PvuII CAG'CTG
Cuts at: 0 59 3993
Size: 59 3934
SacI G_AGCT'C
Cuts at: 0 2753 3993
Size: 2753 1240
ScaI AGT'ACT
Cuts at: 0 1486 2041 2221 3993
Size: 1486 555 180 1772
Fragments arranged by size:
1772 1486 555 180
SfcI C'TryA_G
Cuts at: 0 66 348 2657 2686 2754 3993
Size: 66 282 2309 29 68 1239
Fragments arranged by size:
2309 1239 282 68 66 29
SfoI GGC'GCC
Cuts at: 0 3179 3993
Size: 3179 814
SmlI C'TyrA_G
Cuts at: 0 1521 2817 3376 3568 3579 3993
Size: 1521 1296 559 192 11 414
Fragments arranged by size:
1521 1296 559 414 192 11
SphI G_CATG'C
Cuts at: 0 888 1563 3993
Size: 888 675 2430
Fragments arranged by size:
2430 888 675
SspI AAT'ATT
Cuts at: 0 3297 3993
Size: 3297 696
StyI C'CwwG_G
Cuts at: 0 300 710 1394 1551 1812 2003 2373 2445
Size: 300 410 684 157 261 191 370 72
Cuts at: 2445 3173 3993
Size: 728 820
Fragments arranged by size:
820 728 684 410 370 300 261 191
157 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 315 1282 2761 3262 3993
Size: 315 967 1479 501 731
Fragments arranged by size:
1479 967 731 501 315
TatI w'GTAC_w
Cuts at: 0 1484 2039 2219 3993
Size: 1484 555 180 1774
Fragments arranged by size:
1774 1484 555 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1891 1923 2944 2976 3993
Size: 1891 32 1021 32 1017
Fragments arranged by size:
1891 1021 1017 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1224 2311 2688 3219 3993
Size: 1224 1087 377 531 774
Fragments arranged by size:
1224 1087 774 531 377
ZraI GAC'GTC
Cuts at: 0 653 3993
Size: 653 3340
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaLI ApoI AvaI AvrII BaeI BanI
BanII BbeI BbsI BcgI BciVI BfrBI BglI Bme1580I
BmgBI BmrI BpmI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtsI ClaI CspCI
DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NruI NsiI NspI PasI PciI PflMI
PfoI PpuMI PstI PvuII SacI ScaI SfcI SfoI
SmlI SphI SspI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI ApaI AscI AseI
AsiSI BamHI BbvCI BclI BglII BlpI BmtI BplI
Bpu10I BsaAI BsiEI BsiWI BsmBI BsrGI BstXI BstZ17I
BtgZI DraI EagI EcoRI EcoRV FseI HpaI KpnI
MfeI MluI NheI NotI PacI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI