(Circular) (Six-base) MAPSORT of: pFN21AA0767.seq Check: 8489 from: 1 to: 6439 pFN21AA0767 6439 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2919 4249 278 Size: 53 83 186 936 1383 1330 2468 Fragments arranged by size: 2468 1383 1330 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2697 3617 3782 1937 Size: 760 920 165 4594 Fragments arranged by size: 4594 920 760 165 Acc65I G'GTAC_C Cuts at: 2122 3760 2122 Size: 1638 4801 AclI AA'CG_TT Cuts at: 1563 4566 4939 1563 Size: 3003 373 3063 Fragments arranged by size: 3063 3003 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2138 2420 4502 5886 6154 1632 Size: 506 282 2082 1384 268 1917 Fragments arranged by size: 2082 1917 1384 506 282 268 AfeI AGC'GCT Cuts at: 3218 3218 Size: 6439 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6242 AflIII A'CryG_T Cuts at: 1203 2700 5338 6315 1203 Size: 1497 2638 977 1327 Fragments arranged by size: 2638 1497 1327 977 AgeI A'CCGG_T Cuts at: 5270 5270 Size: 6439 AhdI GACnn_n'nnGTC Cuts at: 5168 5168 Size: 6439 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6439 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6439 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2534 2566 2534 Size: 32 6407 AlwNI CAG_nnn'CTG Cuts at: 2344 2488 3016 3725 4182 5754 2344 Size: 144 528 709 457 1572 3029 Fragments arranged by size: 3029 1572 709 528 457 144 ApaLI G'TGCA_C Cuts at: 3659 4496 5652 3659 Size: 837 1156 4446 Fragments arranged by size: 4446 1156 837 ApoI r'AATT_y Cuts at: 1493 3748 3974 4126 6235 1493 Size: 2255 226 152 2109 1697 Fragments arranged by size: 2255 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4993 160 Size: 872 3961 1606 Fragments arranged by size: 3961 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 AvaI C'yCGr_G Cuts at: 1870 1943 2139 2558 3142 3764 1870 Size: 73 196 419 584 622 4545 Fragments arranged by size: 4545 622 584 419 196 73 BamHI G'GATC_C Cuts at: 3769 4136 3769 Size: 367 6072 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2636 3650 3760 5215 Size: 325 217 627 335 514 1014 110 1455 Cuts at: 5215 618 Size: 1842 Fragments arranged by size: 1842 1455 1014 627 514 335 325 217 110 BanII G_rGCy'C Cuts at: 1391 2298 2859 3758 1391 Size: 907 561 899 4072 Fragments arranged by size: 4072 907 899 561 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2883 928 Size: 971 984 4484 Fragments arranged by size: 4484 984 971 BbvCI CC'TCA_GC Cuts at: 2156 3712 2156 Size: 1556 4883 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4630 4664 1903 Size: 34 50 34 2609 34 3678 Fragments arranged by size: 3678 2609 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2817 4331 5541 2817 Size: 1514 1210 3715 Fragments arranged by size: 3715 1514 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6439 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3044 5050 136 Size: 107 122 71 631 605 1372 2006 1525 Fragments arranged by size: 2006 1525 1372 631 605 122 107 71 BglII A'GATC_T Cuts at: 3351 6434 3351 Size: 3083 3356 BlpI GC'TnA_GC Cuts at: 2427 3856 2427 Size: 1429 5010 Bme1580I G_kGCm'C Cuts at: 1790 2844 3663 4500 5656 1790 Size: 1054 819 837 1156 2573 Fragments arranged by size: 2573 1156 1054 837 819 BmgBI CAC'GTC Cuts at: 1318 2703 1318 Size: 1385 5054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4170 5128 442 Size: 416 53 83 399 24 2753 958 1753 Fragments arranged by size: 2753 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6439 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2396 2984 3202 3205 5099 1239 Size: 1157 588 218 3 1894 2579 Fragments arranged by size: 2579 1894 1157 588 218 3 Bpu10I CC'TnA_GC Cuts at: 2009 2156 2591 3595 3712 2009 Size: 147 435 1004 117 4736 Fragments arranged by size: 4736 1004 435 147 117 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2612 3194 4564 5429 5727 2612 Size: 582 1370 865 298 3324 Fragments arranged by size: 3324 1370 865 582 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3674 5102 882 Size: 617 2175 1428 2219 Fragments arranged by size: 2219 2175 1428 617 BsaAI yAC'GTr Cuts at: 493 1204 2536 3271 4230 493 Size: 711 1332 735 959 2702 Fragments arranged by size: 2702 1332 959 735 711 BsaBI GATnn'nnATC Cuts at: 6433 6433 Size: 6439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2916 3309 3620 4246 Size: 53 83 186 936 1383 393 311 626 Cuts at: 4246 4628 5263 275 Size: 382 635 1451 Fragments arranged by size: 1451 1383 936 635 626 393 382 311 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 2298 2547 3429 4872 5270 5544 5691 6102 Size: 84 249 882 1443 398 274 147 411 Cuts at: 6102 2214 Size: 2551 Fragments arranged by size: 2551 1443 882 411 398 274 249 147 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 1178 Size: 30 165 30 1272 30 4912 Fragments arranged by size: 4912 1272 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2165 2306 2429 2576 3199 3317 1186 Size: 979 141 123 147 623 118 4308 Fragments arranged by size: 4308 979 623 147 141 123 118 BseYI C'CCAG_C Cuts at: 2748 3275 5642 6138 2748 Size: 527 2367 496 3049 Fragments arranged by size: 3049 2367 527 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2408 1163 Size: 573 672 5194 Fragments arranged by size: 5194 672 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3288 3316 3889 4650 5678 1540 Size: 275 186 1287 28 573 761 1028 2301 Fragments arranged by size: 2301 1287 1028 761 573 275 186 28 BsiHKAI G_wGCw'C Cuts at: 2298 3468 3663 4500 4585 5656 2298 Size: 1170 195 837 85 1071 3081 Fragments arranged by size: 3081 1170 1071 837 195 85 BsmI GAATG_Cn' Cuts at: 2102 2737 3950 4043 2102 Size: 635 1213 93 4498 Fragments arranged by size: 4498 1213 635 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2614 2614 Size: 6439 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2844 2859 3468 3663 3758 4500 Size: 399 508 546 15 609 195 95 742 Cuts at: 4500 4585 5656 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 609 546 508 399 195 95 85 15 BspEI T'CCGG_A Cuts at: 2298 6102 2298 Size: 3804 2635 BspHI T'CATG_A Cuts at: 4328 6058 4328 Size: 1730 4709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2592 3794 844 Size: 1748 1202 3489 Fragments arranged by size: 3489 1748 1202 BsrBI CCG'CTC Cuts at: 1394 1823 2356 2521 3640 4326 1394 Size: 429 533 165 1119 686 3507 Fragments arranged by size: 3507 1119 686 533 429 165 BsrDI GCAATG_nn' Cuts at: 65 1248 2467 2709 4934 5108 65 Size: 1183 1219 242 2225 174 1396 Fragments arranged by size: 2225 1396 1219 1183 242 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2644 3283 3287 3311 5083 5270 1700 Size: 110 834 639 4 24 1772 187 2869 Fragments arranged by size: 2869 1772 834 639 187 110 24 4 BsrGI T'GTAC_A Cuts at: 96 2724 96 Size: 2628 3811 BssHII G'CGCG_C Cuts at: 1925 3151 1925 Size: 1226 5213 BssSI C'ACGA_G Cuts at: 3347 3641 4499 5511 3347 Size: 294 858 1012 4275 Fragments arranged by size: 4275 1012 858 294 BstAPI GCAn_nnn'nTGC Cuts at: 794 2386 2395 794 Size: 1592 9 4838 Fragments arranged by size: 4838 1592 9 BstBI TT'CG_AA Cuts at: 3495 5243 3495 Size: 1748 4691 BstEII G'GTnAC_C Cuts at: 1184 2249 1184 Size: 1065 5374 BstYI r'GATC_y Cuts at: 1972 3351 3769 4136 4521 4538 5990 6076 6088 Size: 1379 418 367 385 17 1452 86 12 Cuts at: 6088 6434 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1379 418 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2126 2859 3084 3441 3654 513 Size: 552 1061 733 225 357 213 3298 Fragments arranged by size: 3298 1061 733 552 357 225 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2791 487 Size: 683 56 1565 4135 Fragments arranged by size: 4135 1565 683 56 BtsI GCAGTG_nn' Cuts at: 3955 4749 4769 3955 Size: 794 20 5625 Fragments arranged by size: 5625 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6439 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6404 DraI TTT'AAA Cuts at: 3133 3743 3133 Size: 610 5829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3256 5446 809 Size: 507 1940 2190 1802 Fragments arranged by size: 2190 1940 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 3285 3886 Size: 54 1425 50 275 18 360 1095 601 Cuts at: 3886 4775 6404 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1095 889 601 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3285 3886 1537 Size: 275 1473 601 4090 Fragments arranged by size: 4090 1473 601 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4369 1353 Size: 3016 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2380 2836 5026 5400 5546 387 Size: 1064 138 791 456 2190 374 146 1280 Fragments arranged by size: 2190 1280 1064 791 456 374 146 138 EcoICRI GAG'CTC Cuts at: 2296 2296 Size: 6439 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2396 2420 2984 3202 3205 4502 Size: 393 506 258 24 564 218 3 1297 Cuts at: 4502 5099 5886 6154 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1297 787 597 564 506 393 268 258 218 24 3 EcoNI CCTnn'n_nnAGG Cuts at: 3520 3520 Size: 6439 EcoO109I rG'GnC_Cy Cuts at: 2161 2178 3513 3574 2161 Size: 17 1335 61 5026 Fragments arranged by size: 5026 1335 61 17 EcoRI G'AATT_C Cuts at: 3748 4126 3748 Size: 378 6061 FseI GG_CCGG'CC Cuts at: 3291 3291 Size: 6439 FspI TGC'GCA Cuts at: 4945 4945 Size: 6439 HaeII r_GCGC'y Cuts at: 1678 2476 2598 3029 3220 3228 3409 5586 1678 Size: 798 122 431 191 8 181 2177 2531 Fragments arranged by size: 2531 2177 798 431 191 181 122 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2233 2265 2675 2707 3343 Size: 32 135 32 661 32 410 32 636 Cuts at: 3343 3375 5080 5112 5154 5186 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1705 661 636 410 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2607 3618 3783 4029 669 Size: 988 281 669 1011 165 246 3079 Fragments arranged by size: 3079 1011 988 669 281 246 165 HindIII A'AGCT_T Cuts at: 748 748 Size: 6439 HpaI GTT'AAC Cuts at: 2607 4029 2607 Size: 1422 5017 KpnI G_GTAC'C Cuts at: 2126 3764 2126 Size: 1638 4801 MfeI C'AATT_G Cuts at: 4038 4038 Size: 6439 MluI A'CGCG_T Cuts at: 6315 6315 Size: 6439 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1998 2706 5553 5737 1305 Size: 693 708 2847 184 2007 Fragments arranged by size: 2847 2007 708 693 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2192 10 Size: 54 1425 343 360 4257 Fragments arranged by size: 4257 1425 360 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3666 4397 4756 4915 518 Size: 648 2500 731 359 159 2042 Fragments arranged by size: 2500 2042 731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2128 2362 2905 3382 3716 3855 4532 5680 Size: 522 234 543 477 334 139 677 1148 Cuts at: 5680 5925 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 543 522 477 334 245 234 139 NaeI GCC'GGC Cuts at: 3289 3289 Size: 6439 NcoI C'CATG_G Cuts at: 513 1065 3654 513 Size: 552 2589 3298 Fragments arranged by size: 3298 2589 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6439 NgoMIV G'CCGG_C Cuts at: 3287 3287 Size: 6439 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6439 NotI GC'GGCC_GC Cuts at: 3886 3886 Size: 6439 NspI r_CATG'y Cuts at: 1132 1229 3797 5342 1132 Size: 97 2568 1545 2229 Fragments arranged by size: 2568 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6439 PciI A'CATG_T Cuts at: 5338 5338 Size: 6439 PflMI CCAn_nnn'nTGG Cuts at: 1108 2817 1108 Size: 1709 4730 PfoI T'CCnGG_A Cuts at: 2202 2961 2202 Size: 759 5680 PmeI GTTT'AAAC Cuts at: 3743 3743 Size: 6439 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4508 4540 6021 6053 4508 Size: 32 1481 32 4894 Fragments arranged by size: 4894 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2161 2178 3513 3574 2161 Size: 17 1335 61 5026 Fragments arranged by size: 5026 1335 61 17 PshAI GACnn'nnGTC Cuts at: 1613 3312 1613 Size: 1699 4740 PsiI TTA'TAA Cuts at: 4009 4009 Size: 6439 PstI C_TGCA'G Cuts at: 830 2505 3249 3791 830 Size: 1675 744 542 3478 Fragments arranged by size: 3478 1675 744 542 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6439 PvuII CAG'CTG Cuts at: 2905 2905 Size: 6439 SacI G_AGCT'C Cuts at: 2298 2298 Size: 6439 SacII CC_GC'GG Cuts at: 2129 2129 Size: 6439 SalI G'TCGA_C Cuts at: 1936 3616 3781 1936 Size: 1680 165 4594 Fragments arranged by size: 4594 1680 165 SanDI GG'GwC_CC Cuts at: 2161 2178 2161 Size: 17 6422 SbfI CC_TGCA'GG Cuts at: 3791 3791 Size: 6439 ScaI AGT'ACT Cuts at: 4687 6399 4687 Size: 1712 4727 SfcI C'TryA_G Cuts at: 826 1045 2501 3245 3787 4922 5603 5794 826 Size: 219 1456 744 542 1135 681 191 1471 Fragments arranged by size: 1471 1456 1135 744 681 542 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6439 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6439 SmaI CCC'GGG Cuts at: 1872 2141 3766 1872 Size: 269 1625 4545 Fragments arranged by size: 4545 1625 269 SmlI C'TyrA_G Cuts at: 820 1017 1943 2627 3209 4543 5444 5706 820 Size: 197 926 684 582 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 684 582 262 197 SnaBI TAC'GTA Cuts at: 493 3271 4230 493 Size: 2778 959 2702 Fragments arranged by size: 2778 2702 959 SpeI A'CTAG_T Cuts at: 152 152 Size: 6439 SphI G_CATG'C Cuts at: 1132 3797 1132 Size: 2665 3774 SspI AAT'ATT Cuts at: 5 52 4363 5 Size: 47 4311 2081 Fragments arranged by size: 4311 2081 47 StuI AGG'CCT Cuts at: 1517 3674 1517 Size: 2157 4282 StyI C'CwwG_G Cuts at: 513 1065 3654 3878 513 Size: 552 2589 224 3074 Fragments arranged by size: 3074 2589 552 224 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3644 4466 4483 4636 4821 5266 325 Size: 1255 2064 822 17 153 185 445 1498 Fragments arranged by size: 2064 1498 1255 822 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 3567 3696 Size: 274 80 33 51 1443 747 843 129 Cuts at: 3696 4685 6397 96 Size: 989 1712 138 Fragments arranged by size: 1712 1443 989 843 747 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6399 6431 6399 Size: 32 6407 Tth111I GACn'n_nGTC Cuts at: 1316 2887 3256 1316 Size: 1571 369 4499 Fragments arranged by size: 4499 1571 369 XbaI T'CTAG_A Cuts at: 3775 3775 Size: 6439 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2459 2459 Size: 6439 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6439 XmaI C'CCGG_G Cuts at: 1870 2139 3764 1870 Size: 269 1625 4545 Fragments arranged by size: 4545 1625 269 XmnI GAAnn'nnTTC Cuts at: 2510 2939 2981 4568 2510 Size: 429 42 1587 4381 Fragments arranged by size: 4381 1587 429 42 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2917 4247 276 Size: 53 83 186 936 1383 1330 2468 Fragments arranged by size: 2468 1383 1330 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI