Restriction map for
pFN21AA0676
(Linear) (Six-base) MAPSORT of: KIAA0676.seq Check: 5647 from: 1 to: 3750
KIAA0676.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2091 3750
Size: 2091 1659
AccI GT'mk_AC
Cuts at: 0 209 2063 3575 3750
Size: 209 1854 1512 175
Fragments arranged by size:
1854 1512 209 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 850 1387 1537 3280 3750
Size: 445 405 537 150 1743 470
Fragments arranged by size:
1743 537 470 445 405 150
AflIII A'CryG_T
Cuts at: 0 607 3107 3452 3750
Size: 607 2500 345 298
Fragments arranged by size:
2500 607 345 298
AhdI GACnn_n'nnGTC
Cuts at: 0 3453 3750
Size: 3453 297
AleI CACnn'nnGTG
Cuts at: 0 1891 2240 3750
Size: 1891 349 1510
Fragments arranged by size:
1891 1510 349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2258 3750
Size: 2258 1492
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3691 3723 3750
Size: 3691 32 27
Fragments arranged by size:
3691 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 291 491 756 1362 1977 2142 2370 3138
Size: 291 200 265 606 615 165 228 768
Cuts at: 3138 3233 3426 3609 3750
Size: 95 193 183 141
Fragments arranged by size:
768 615 606 291 265 228 200 193
183 165 141 95
ApoI r'AATT_y
Cuts at: 0 2317 3750
Size: 2317 1433
AvaI C'yCGr_G
Cuts at: 0 400 640 848 1594 1684 3750
Size: 400 240 208 746 90 2066
Fragments arranged by size:
2066 746 400 240 208 90
AvrII C'CTAG_G
Cuts at: 0 793 3750
Size: 793 2957
BanI G'GyrC_C
Cuts at: 0 130 1341 3568 3750
Size: 130 1211 2227 182
Fragments arranged by size:
2227 1211 182 130
BanII G_rGCy'C
Cuts at: 0 13 677 1905 1934 2297 2800 3412 3490
Size: 13 664 1228 29 363 503 612 78
Cuts at: 3490 3750
Size: 260
Fragments arranged by size:
1228 664 612 503 363 260 78 29
13
BbeI G_GCGC'C
Cuts at: 0 1345 3750
Size: 1345 2405
BbsI GAAGACnn'nnnn_
Cuts at: 0 112 1247 2345 3125 3509 3750
Size: 112 1135 1098 780 384 241
Fragments arranged by size:
1135 1098 780 384 241 112
BbvCI CC'TCA_GC
Cuts at: 0 1309 2026 3750
Size: 1309 717 1724
Fragments arranged by size:
1724 1309 717
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1675 1709 2986 3020 3750
Size: 1675 34 1277 34 730
Fragments arranged by size:
1675 1277 730 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1534 3750
Size: 1534 2216
BclI T'GATC_A
Cuts at: 0 2693 3750
Size: 2693 1057
BglI GCCn_nnn'nGGC
Cuts at: 0 166 739 804 1414 2824 3160 3750
Size: 166 573 65 610 1410 336 590
Fragments arranged by size:
1410 610 590 573 336 166 65
BglII A'GATC_T
Cuts at: 0 2880 3750
Size: 2880 870
BlpI GC'TnA_GC
Cuts at: 0 7 419 1607 2251 3750
Size: 7 412 1188 644 1499
Fragments arranged by size:
1499 1188 644 412 7
Bme1580I G_kGCm'C
Cuts at: 0 133 162 509 1509 3750
Size: 133 29 347 1000 2241
Fragments arranged by size:
2241 1000 347 133 29
BmgBI CAC'GTC
Cuts at: 0 141 3750
Size: 141 3609
BmrI ACTGGGnnnn_n'
Cuts at: 0 267 2517 3750
Size: 267 2250 1233
Fragments arranged by size:
2250 1233 267
BmtI G_CTAG'C
Cuts at: 0 2501 3750
Size: 2501 1249
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2237 2868 3750
Size: 2237 631 882
Fragments arranged by size:
2237 882 631
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 268 634 796 1056 1165 1974 2479
Size: 138 130 366 162 260 109 809 505
Cuts at: 2479 2581 2842 3750
Size: 102 261 908
Fragments arranged by size:
908 809 505 366 261 260 162 138
130 109 102
Bpu10I CC'TnA_GC
Cuts at: 0 1309 2026 3750
Size: 1309 717 1724
Fragments arranged by size:
1724 1309 717
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2743 2894 3658 3750
Size: 2743 151 764 92
Fragments arranged by size:
2743 764 151 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 700 830 1658 3308 3750
Size: 700 130 828 1650 442
Fragments arranged by size:
1650 828 700 442 130
BsaBI GATnn'nnATC
Cuts at: 0 970 2885 3750
Size: 970 1915 865
Fragments arranged by size:
1915 970 865
BsaHI Gr'CG_yC
Cuts at: 0 84 845 934 1342 2123 3750
Size: 84 761 89 408 781 1627
Fragments arranged by size:
1627 781 761 408 89 84
BsaWI w'CCGG_w
Cuts at: 0 2570 2985 3750
Size: 2570 415 765
Fragments arranged by size:
2570 765 415
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1892 1922 2967 2997 3691 3721 3750
Size: 1892 30 1045 30 694 30 29
Fragments arranged by size:
1892 1045 694 30 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1021 1256 1450 1546 1666 1840 1843
Size: 31 990 235 194 96 120 174 3
Cuts at: 1843 2213 2421 2844 2858 2968 2975 2980 3259
Size: 370 208 423 14 110 7 5 279
Cuts at: 3259 3750
Size: 491
Fragments arranged by size:
990 491 423 370 279 235 208 194
174 120 110 96 31 14 7 5
3
BseYI C'CCAG_C
Cuts at: 0 564 1084 1260 1296 1710 2142 2178 2545
Size: 564 520 176 36 414 432 36 367
Cuts at: 2545 2787 3303 3419 3750
Size: 242 516 116 331
Fragments arranged by size:
564 520 516 432 414 367 331 242
176 116 36 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 610 906 934 1180 1654 2535 3750
Size: 169 441 296 28 246 474 881 1215
Fragments arranged by size:
1215 881 474 441 296 246 169 28
BsiEI CG_ry'CG
Cuts at: 0 2535 3750
Size: 2535 1215
BsiHKAI G_wGCw'C
Cuts at: 0 677 1691 1808 1934 2297 2768 3750
Size: 677 1014 117 126 363 471 982
Fragments arranged by size:
1014 982 677 471 363 126 117
BsmBI CGTCTCn'nnnn_
Cuts at: 0 383 3034 3750
Size: 383 2651 716
Fragments arranged by size:
2651 716 383
Bsp1286I G_dGCh'C
Cuts at: 0 13 133 162 509 677 1509 1691 1808
Size: 13 120 29 347 168 832 182 117
Cuts at: 1808 1905 1934 2297 2768 2800 3412 3490 3750
Size: 97 29 363 471 32 612 78 260
Fragments arranged by size:
832 612 471 363 347 260 182 168
120 117 97 78 32 29 29 13
BspEI T'CCGG_A
Cuts at: 0 2985 3750
Size: 2985 765
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 1676 2091 3750
Size: 383 1293 415 1659
Fragments arranged by size:
1659 1293 415 383
BsrBI CCG'CTC
Cuts at: 0 54 900 1676 2726 3750
Size: 54 846 776 1050 1024
Fragments arranged by size:
1050 1024 846 776 54
BsrDI GCAATG_nn'
Cuts at: 0 1790 3502 3750
Size: 1790 1712 248
Fragments arranged by size:
1790 1712 248
BsrFI r'CCGG_y
Cuts at: 0 2530 3750
Size: 2530 1220
BssHII G'CGCG_C
Cuts at: 0 3556 3750
Size: 3556 194
BssSI C'ACGA_G
Cuts at: 0 124 583 1935 3750
Size: 124 459 1352 1815
Fragments arranged by size:
1815 1352 459 124
BstBI TT'CG_AA
Cuts at: 0 1925 3006 3750
Size: 1925 1081 744
Fragments arranged by size:
1925 1081 744
BstEII G'GTnAC_C
Cuts at: 0 1044 3750
Size: 1044 2706
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1236 1896 2493 3750
Size: 1236 660 597 1257
Fragments arranged by size:
1257 1236 660 597
BstYI r'GATC_y
Cuts at: 0 1166 2880 2886 3051 3605 3750
Size: 1166 1714 6 165 554 145
Fragments arranged by size:
1714 1166 554 165 145 6
Bsu36I CC'TnA_GG
Cuts at: 0 452 1036 1268 2329 3750
Size: 452 584 232 1061 1421
Fragments arranged by size:
1421 1061 584 452 232
BtgI C'CryG_G
Cuts at: 0 93 1391 2051 2736 3125 3158 3750
Size: 93 1298 660 685 389 33 592
Fragments arranged by size:
1298 685 660 592 389 93 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 339 2276 3135 3750
Size: 339 1937 859 615
Fragments arranged by size:
1937 859 615 339
BtsI GCAGTG_nn'
Cuts at: 0 598 1827 3750
Size: 598 1229 1923
Fragments arranged by size:
1923 1229 598
DraIII CAC_nnn'GTG
Cuts at: 0 929 3750
Size: 929 2821
DrdI GACnn_nn'nnGTC
Cuts at: 0 205 3750
Size: 205 3545
EaeI y'GGCC_r
Cuts at: 0 29 731 740 805 909 960 1643 2111
Size: 29 702 9 65 104 51 683 468
Cuts at: 2111 2399 2528 2532 3161 3750
Size: 288 129 4 629 589
Fragments arranged by size:
702 683 629 589 468 288 129 104
65 51 29 9 4
EagI C'GGCC_G
Cuts at: 0 2532 3750
Size: 2532 1218
EarI CTCTTCn'nnn_
Cuts at: 0 356 640 682 1378 1564 1922 2026 2447
Size: 356 284 42 696 186 358 104 421
Cuts at: 2447 2924 3649 3750
Size: 477 725 101
Fragments arranged by size:
725 696 477 421 358 356 284 186
104 101 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3350 3750
Size: 3350 400
EcoICRI GAG'CTC
Cuts at: 0 675 1932 2295 3750
Size: 675 1257 363 1455
Fragments arranged by size:
1455 1257 675 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 138 268 445 634 796 850 1056 1165
Size: 138 130 177 189 162 54 206 109
Cuts at: 1165 1387 1537 1974 2479 2581 2842 3280 3750
Size: 222 150 437 505 102 261 438 470
Fragments arranged by size:
505 470 438 437 261 222 206 189
177 162 150 138 130 109 102 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1039 3750
Size: 1039 2711
EcoO109I rG'GnC_Cy
Cuts at: 0 396 786 1359 1398 1568 1974 2463 2541
Size: 396 390 573 39 170 406 489 78
Cuts at: 2541 2742 3135 3750
Size: 201 393 615
Fragments arranged by size:
615 573 489 406 396 393 390 201
170 78 39
EcoRV GAT'ATC
Cuts at: 0 318 3750
Size: 318 3432
FseI GG_CCGG'CC
Cuts at: 0 2534 3750
Size: 2534 1216
FspI TGC'GCA
Cuts at: 0 1334 3750
Size: 1334 2416
HaeII r_GCGC'y
Cuts at: 0 1345 2051 3750
Size: 1345 706 1699
Fragments arranged by size:
1699 1345 706
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1572 1604 2839 2871 2967 2999 3036 3068
Size: 1572 32 1235 32 96 32 37 32
Cuts at: 3068 3439 3471 3662 3694 3750
Size: 371 32 191 32 56
Fragments arranged by size:
1572 1235 371 191 96 56 37 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 537 2064 2205 3576 3750
Size: 537 1527 141 1371 174
Fragments arranged by size:
1527 1371 537 174 141
KasI G'GCGC_C
Cuts at: 0 1341 3750
Size: 1341 2409
MluI A'CGCG_T
Cuts at: 0 607 3750
Size: 607 3143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1259 3750
Size: 1001 258 2491
Fragments arranged by size:
2491 1001 258
MscI TGG'CCA
Cuts at: 0 31 733 742 962 1645 3163 3750
Size: 31 702 9 220 683 1518 587
Fragments arranged by size:
1518 702 683 587 220 31 9
MslI CAynn'nnrTG
Cuts at: 0 652 1891 2089 2173 2240 3457 3750
Size: 652 1239 198 84 67 1217 293
Fragments arranged by size:
1239 1217 652 293 198 84 67
MspA1I CmG'CkG
Cuts at: 0 753 2139 3307 3395 3423 3750
Size: 753 1386 1168 88 28 327
Fragments arranged by size:
1386 1168 753 327 88 28
NaeI GCC'GGC
Cuts at: 0 2532 3750
Size: 2532 1218
NarI GG'CG_CC
Cuts at: 0 1342 3750
Size: 1342 2408
NcoI C'CATG_G
Cuts at: 0 1391 3125 3750
Size: 1391 1734 625
Fragments arranged by size:
1734 1391 625
NgoMIV G'CCGG_C
Cuts at: 0 2530 3750
Size: 2530 1220
NheI G'CTAG_C
Cuts at: 0 2497 3750
Size: 2497 1253
NspI r_CATG'y
Cuts at: 0 1485 1869 2661 3111 3456 3750
Size: 1485 384 792 450 345 294
Fragments arranged by size:
1485 792 450 384 345 294
PasI CC'CwG_GG
Cuts at: 0 384 890 1356 2618 2900 3227 3750
Size: 384 506 466 1262 282 327 523
Fragments arranged by size:
1262 523 506 466 384 327 282
PciI A'CATG_T
Cuts at: 0 3107 3452 3750
Size: 3107 345 298
Fragments arranged by size:
3107 345 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 2388 3378 3750
Size: 2388 990 372
Fragments arranged by size:
2388 990 372
PfoI T'CCnGG_A
Cuts at: 0 773 2117 2591 2669 3750
Size: 773 1344 474 78 1081
Fragments arranged by size:
1344 1081 773 474 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3691 3723 3750
Size: 3691 32 27
Fragments arranged by size:
3691 32 27
PpuMI rG'GwC_Cy
Cuts at: 0 396 1398 1974 2463 2541 2742 3135 3750
Size: 396 1002 576 489 78 201 393 615
Fragments arranged by size:
1002 615 576 489 396 393 201 78
PshAI GACnn'nnGTC
Cuts at: 0 1051 3750
Size: 1051 2699
PstI C_TGCA'G
Cuts at: 0 80 380 487 2102 2152 2277 3312 3547
Size: 80 300 107 1615 50 125 1035 235
Cuts at: 3547 3750
Size: 203
Fragments arranged by size:
1615 1035 300 235 203 125 107 80
50
PvuII CAG'CTG
Cuts at: 0 753 2139 3307 3423 3750
Size: 753 1386 1168 116 327
Fragments arranged by size:
1386 1168 753 327 116
SacI G_AGCT'C
Cuts at: 0 677 1934 2297 3750
Size: 677 1257 363 1453
Fragments arranged by size:
1453 1257 677 363
SalI G'TCGA_C
Cuts at: 0 2062 3574 3750
Size: 2062 1512 176
Fragments arranged by size:
2062 1512 176
SanDI GG'GwC_CC
Cuts at: 0 2541 3750
Size: 2541 1209
SapI GCTCTTCn'nnn_
Cuts at: 0 640 682 1378 1922 2447 2924 3750
Size: 640 42 696 544 525 477 826
Fragments arranged by size:
826 696 640 544 525 477 42
SbfI CC_TGCA'GG
Cuts at: 0 2102 3750
Size: 2102 1648
ScaI AGT'ACT
Cuts at: 0 2506 3750
Size: 2506 1244
SfcI C'TryA_G
Cuts at: 0 76 376 483 2098 2148 2273 3308 3543
Size: 76 300 107 1615 50 125 1035 235
Cuts at: 3543 3750
Size: 207
Fragments arranged by size:
1615 1035 300 235 207 125 107 76
50
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 739 804 3750
Size: 739 65 2946
Fragments arranged by size:
2946 739 65
SfoI GGC'GCC
Cuts at: 0 1343 3750
Size: 1343 2407
SmaI CCC'GGG
Cuts at: 0 402 1596 3750
Size: 402 1194 2154
Fragments arranged by size:
2154 1194 402
SmlI C'TyrA_G
Cuts at: 0 2758 2909 3673 3750
Size: 2758 151 764 77
Fragments arranged by size:
2758 764 151 77
SphI G_CATG'C
Cuts at: 0 1869 3750
Size: 1869 1881
StuI AGG'CCT
Cuts at: 0 1834 3541 3750
Size: 1834 1707 209
Fragments arranged by size:
1834 1707 209
StyI C'CwwG_G
Cuts at: 0 793 1391 1401 1409 1418 1914 2387 3017
Size: 793 598 10 8 9 496 473 630
Cuts at: 3017 3125 3750
Size: 108 625
Fragments arranged by size:
793 630 625 598 496 473 108 10
9 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 585 3750
Size: 33 552 3165
Fragments arranged by size:
3165 552 33
TatI w'GTAC_w
Cuts at: 0 1634 2504 3750
Size: 1634 870 1246
Fragments arranged by size:
1634 1246 870
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1215 1247 1892 1924 3750
Size: 1215 32 645 32 1826
Fragments arranged by size:
1826 1215 645 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 291 3627 3750
Size: 291 3336 123
Fragments arranged by size:
3336 291 123
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 2175 2409 2454 3156 3733 3750
Size: 162 2013 234 45 702 577 17
Fragments arranged by size:
2013 702 577 234 162 45 17
XmaI C'CCGG_G
Cuts at: 0 400 1594 3750
Size: 400 1194 2156
Fragments arranged by size:
2156 1194 400
XmnI GAAnn'nnTTC
Cuts at: 0 1372 3199 3750
Size: 1372 1827 551
Fragments arranged by size:
1827 1372 551
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FseI FspI HaeII
Hin4I HincII KasI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PpiI PpuMI PshAI PstI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApaI ApaLI
AscI AseI AsiSI BaeI BamHI BfrBI BsaAI BsiWI
BsmI BspHI BsrGI BstAPI BstZ17I ClaI CspCI DraI
EcoRI FalI FspAI HindIII HpaI KpnI MfeI NdeI
NotI NruI NsiI PacI PmeI PmlI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI ZraI