(Circular) (Six-base) MAPSORT of: pFN21AA0603.seq Check: 9660 from: 1 to: 8602 pFN21AA0603 8602 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6412 278 Size: 53 83 186 936 4876 2468 Fragments arranged by size: 4876 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4171 4774 5945 1937 Size: 2234 603 1171 4594 Fragments arranged by size: 4594 2234 1171 603 Acc65I G'GTAC_C Cuts at: 5923 5923 Size: 8602 AclI AA'CG_TT Cuts at: 1563 3878 6729 7102 1563 Size: 2315 2851 373 3063 Fragments arranged by size: 3063 2851 2315 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2735 3298 3786 4200 5321 5367 5549 6665 Size: 1103 563 488 414 1121 46 182 1116 Cuts at: 6665 8049 8317 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1121 1116 1103 563 488 414 268 182 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8405 AflIII A'CryG_T Cuts at: 1203 3589 3927 7501 8478 1203 Size: 2386 338 3574 977 1327 Fragments arranged by size: 3574 2386 1327 977 338 AgeI A'CCGG_T Cuts at: 7433 7433 Size: 8602 AhdI GACnn_n'nnGTC Cuts at: 2455 5152 7331 2455 Size: 2697 2179 3726 Fragments arranged by size: 3726 2697 2179 AleI CACnn'nnGTG Cuts at: 1166 2966 5073 1166 Size: 1800 2107 4695 Fragments arranged by size: 4695 2107 1800 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4902 5541 1184 Size: 3718 639 4245 Fragments arranged by size: 4245 3718 639 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2053 2021 Size: 32 8570 AlwNI CAG_nnn'CTG Cuts at: 2713 3227 4798 6345 7917 2713 Size: 514 1571 1547 1572 3398 Fragments arranged by size: 3398 1572 1571 1547 514 ApaLI G'TGCA_C Cuts at: 3593 6659 7815 3593 Size: 3066 1156 4380 Fragments arranged by size: 4380 3066 1156 ApoI r'AATT_y Cuts at: 1493 3145 3967 4162 4785 5102 5911 6137 6289 Size: 1652 822 195 623 317 809 226 152 Cuts at: 6289 8398 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1652 822 809 623 317 226 195 152 AseI AT'TA_AT Cuts at: 160 1032 7156 160 Size: 872 6124 1606 Fragments arranged by size: 6124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8602 AvaI C'yCGr_G Cuts at: 1870 1943 2057 2087 2210 2241 2308 2453 2783 Size: 73 114 30 123 31 67 145 330 Cuts at: 2783 2880 5927 1870 Size: 97 3047 4545 Fragments arranged by size: 4545 3047 330 145 123 114 97 73 67 31 30 BamHI G'GATC_C Cuts at: 5932 6299 5932 Size: 367 8235 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2230 2779 2822 5923 7378 Size: 325 217 627 443 549 43 3101 1455 Cuts at: 7378 618 Size: 1842 Fragments arranged by size: 3101 1842 1455 627 549 443 325 217 43 BanII G_rGCy'C Cuts at: 1391 2058 3988 4022 5921 1391 Size: 667 1930 34 1899 4072 Fragments arranged by size: 4072 1930 1899 667 34 BbeI G_GCGC'C Cuts at: 2234 2234 Size: 8602 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3590 3922 4082 4091 928 Size: 971 1691 332 160 9 5439 Fragments arranged by size: 5439 1691 971 332 160 9 BbvCI CC'TCA_GC Cuts at: 3865 4010 4369 3865 Size: 145 359 8098 Fragments arranged by size: 8098 359 145 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2921 2955 6793 6827 1903 Size: 34 984 34 3838 34 3678 Fragments arranged by size: 3838 3678 984 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5018 6494 7704 5018 Size: 1476 1210 5916 Fragments arranged by size: 5916 1476 1210 BclI T'GATC_A Cuts at: 1548 2430 1548 Size: 882 7720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7213 136 Size: 107 122 71 631 605 5541 1525 Fragments arranged by size: 5541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5209 8597 5209 Size: 3388 5214 BlpI GC'TnA_GC Cuts at: 2076 2261 4890 6019 2076 Size: 185 2629 1129 4659 Fragments arranged by size: 4659 2629 1129 185 Bme1580I G_kGCm'C Cuts at: 1790 2318 2784 3597 3925 6663 7819 1790 Size: 528 466 813 328 2738 1156 2573 Fragments arranged by size: 2738 2573 1156 813 528 466 328 BmgBI CAC'GTC Cuts at: 1318 3040 1318 Size: 1722 6880 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3027 5241 6333 Size: 416 53 83 399 24 1610 2214 1092 Cuts at: 6333 7291 442 Size: 958 1753 Fragments arranged by size: 2214 1753 1610 1092 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8602 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4631 Size: 8602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 3204 4006 4069 4601 5073 5633 7262 Size: 833 1132 802 63 532 472 560 1629 Cuts at: 7262 1239 Size: 2579 Fragments arranged by size: 2579 1629 1132 833 802 560 532 472 63 Bpu10I CC'TnA_GC Cuts at: 2895 3865 3987 4010 4369 2895 Size: 970 122 23 359 7128 Fragments arranged by size: 7128 970 359 122 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2644 3715 6727 7592 7890 2644 Size: 1071 3012 865 298 3356 Fragments arranged by size: 3356 3012 1071 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2949 3419 5723 5847 7265 882 Size: 617 1450 470 2304 124 1418 2219 Fragments arranged by size: 2304 2219 1450 1418 617 470 124 BsaAI yAC'GTr Cuts at: 493 1204 3592 6393 493 Size: 711 2388 2801 2702 Fragments arranged by size: 2801 2702 2388 711 BsaBI GATnn'nnATC Cuts at: 8596 8596 Size: 8602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2231 2586 6409 Size: 53 83 186 936 534 164 355 3823 Cuts at: 6409 6791 7426 275 Size: 382 635 1451 Fragments arranged by size: 3823 1451 936 635 534 382 355 186 164 83 53 BsaWI w'CCGG_w Cuts at: 2740 5751 5802 7035 7433 7707 7854 8265 2740 Size: 3011 51 1233 398 274 147 411 3077 Fragments arranged by size: 3077 3011 1233 411 398 274 147 51 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2927 2957 1178 Size: 30 165 30 1524 30 6823 Fragments arranged by size: 6823 1524 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2825 3151 4293 4457 4537 4794 1186 Size: 1639 326 1142 164 80 257 4994 Fragments arranged by size: 4994 1639 1142 326 257 164 80 BseYI C'CCAG_C Cuts at: 2015 2300 2495 2567 3003 7805 8301 2015 Size: 285 195 72 436 4802 496 2316 Fragments arranged by size: 4802 2316 496 436 285 195 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2681 2971 4054 5051 1163 Size: 573 945 290 1083 997 4714 Fragments arranged by size: 4714 1083 997 945 573 290 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6052 6813 7841 1540 Size: 275 186 4051 761 1028 2301 Fragments arranged by size: 4051 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2262 2367 3597 4022 6663 6748 7819 2262 Size: 105 1230 425 2641 85 1071 3045 Fragments arranged by size: 3045 2641 1230 1071 425 105 85 BsmI GAATG_Cn' Cuts at: 2022 6113 6206 2022 Size: 4091 93 4418 Fragments arranged by size: 4418 4091 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2074 2074 Size: 8602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2262 2318 2367 2784 3597 3925 Size: 399 268 204 56 49 417 813 328 Cuts at: 3925 3988 4022 5921 6663 6748 7819 1391 Size: 63 34 1899 742 85 1071 2174 Fragments arranged by size: 2174 1899 1071 813 742 417 399 328 268 204 85 63 56 49 34 BspEI T'CCGG_A Cuts at: 2740 5751 5802 8265 2740 Size: 3011 51 2463 3077 Fragments arranged by size: 3077 3011 2463 51 BspHI T'CATG_A Cuts at: 5194 6491 8221 5194 Size: 1297 1730 5575 Fragments arranged by size: 5575 1730 1297 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2854 4268 5957 844 Size: 2010 1414 1689 3489 Fragments arranged by size: 3489 2010 1689 1414 BsrBI CCG'CTC Cuts at: 1394 1823 2899 6489 1394 Size: 429 1076 3590 3507 Fragments arranged by size: 3590 3507 1076 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4273 4380 7097 7271 65 Size: 1183 3025 107 2717 174 1396 Fragments arranged by size: 3025 2717 1396 1183 174 107 BsrFI r'CCGG_y Cuts at: 1700 1810 2233 2963 7246 7433 1700 Size: 110 423 730 4283 187 2869 Fragments arranged by size: 4283 2869 730 423 187 110 BsrGI T'GTAC_A Cuts at: 96 4062 96 Size: 3966 4636 BssHII G'CGCG_C Cuts at: 1925 2265 1925 Size: 340 8262 BssSI C'ACGA_G Cuts at: 2984 4907 6662 7674 2984 Size: 1923 1755 1012 3912 Fragments arranged by size: 3912 1923 1755 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3385 794 Size: 2591 6011 BstBI TT'CG_AA Cuts at: 2610 3913 4567 7406 2610 Size: 1303 654 2839 3806 Fragments arranged by size: 3806 2839 1303 654 BstEII G'GTnAC_C Cuts at: 1184 2632 1184 Size: 1448 7154 BstYI r'GATC_y Cuts at: 1972 2187 2718 3698 4311 5209 5932 6299 6684 Size: 215 531 980 613 898 723 367 385 Cuts at: 6684 6701 8153 8239 8251 8597 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 980 898 723 613 531 385 367 346 215 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3557 4337 3557 Size: 780 7822 BtgI C'CryG_G Cuts at: 513 1065 2004 4437 5817 513 Size: 552 939 2433 1380 3298 Fragments arranged by size: 3298 2433 1380 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7863 Fragments arranged by size: 7863 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3661 4128 4209 4884 6118 6912 6932 2962 Size: 699 467 81 675 1234 794 20 4632 Fragments arranged by size: 4632 1234 794 699 675 467 81 20 ClaI AT'CG_AT Cuts at: 1554 2670 1554 Size: 1116 7486 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8567 DraI TTT'AAA Cuts at: 3151 5906 3151 Size: 2755 5847 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7609 809 Size: 507 6293 1802 Fragments arranged by size: 6293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2181 2328 2544 Size: 54 1425 50 275 18 351 147 216 Cuts at: 2544 6049 6938 8567 8 Size: 3505 889 1629 43 Fragments arranged by size: 3505 1629 1425 889 351 275 216 147 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6049 1537 Size: 275 4237 4090 Fragments arranged by size: 4237 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3083 3882 4752 5073 6532 1353 Size: 1730 799 870 321 1459 3423 Fragments arranged by size: 3423 1730 1459 870 799 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7189 7563 7709 387 Size: 1064 138 5600 374 146 1280 Fragments arranged by size: 5600 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4020 4020 Size: 8602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2735 3204 3298 3786 4006 4069 Size: 393 440 663 469 94 488 220 63 Cuts at: 4069 4200 4601 5073 5321 5367 5549 5633 6665 Size: 131 401 472 248 46 182 84 1032 Cuts at: 6665 7262 8049 8317 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1032 787 663 597 488 472 469 440 401 393 268 248 220 182 131 94 84 63 46 EcoNI CCTnn'n_nnAGG Cuts at: 2432 2807 2432 Size: 375 8227 EcoO109I rG'GnC_Cy Cuts at: 2304 2653 2755 2787 3608 4248 2304 Size: 349 102 32 821 640 6658 Fragments arranged by size: 6658 821 640 349 102 32 EcoRI G'AATT_C Cuts at: 4162 5911 6289 4162 Size: 1749 378 6475 Fragments arranged by size: 6475 1749 378 EcoRV GAT'ATC Cuts at: 3157 3157 Size: 8602 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3718 5411 3718 Size: 1693 6909 FspI TGC'GCA Cuts at: 7108 7108 Size: 8602 HaeII r_GCGC'y Cuts at: 1678 2234 2301 2713 7749 1678 Size: 556 67 412 5036 2531 Fragments arranged by size: 5036 2531 556 412 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2235 2267 2441 2473 2863 Size: 32 135 32 663 32 174 32 390 Cuts at: 2863 2895 2927 2959 3110 3142 3146 3178 3397 Size: 32 32 32 151 32 4 32 219 Cuts at: 3397 3429 3884 3916 4701 4733 5138 5170 7243 Size: 32 455 32 785 32 405 32 2073 Cuts at: 7243 7275 7317 7349 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2073 785 663 455 405 390 219 174 151 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 4775 5946 6192 669 Size: 988 281 2837 1171 246 3079 Fragments arranged by size: 3079 2837 1171 988 281 246 HindIII A'AGCT_T Cuts at: 748 4150 748 Size: 3402 5200 HpaI GTT'AAC Cuts at: 6192 6192 Size: 8602 KasI G'GCGC_C Cuts at: 2230 2230 Size: 8602 KpnI G_GTAC'C Cuts at: 5927 5927 Size: 8602 MfeI C'AATT_G Cuts at: 5833 6201 5833 Size: 368 8234 MluI A'CGCG_T Cuts at: 8478 8478 Size: 8602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3813 3921 4457 7716 7900 1305 Size: 2508 108 536 3259 184 2007 Fragments arranged by size: 3259 2508 2007 536 184 108 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6780 Fragments arranged by size: 6780 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2966 5073 5529 6560 6919 7078 518 Size: 648 1800 2107 456 1031 359 159 2042 Fragments arranged by size: 2107 2042 1800 1031 648 456 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 3565 4453 4972 5179 5819 6018 6695 Size: 413 1546 888 519 207 640 199 677 Cuts at: 6695 7843 8088 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1546 1148 888 677 640 519 413 245 207 199 NaeI GCC'GGC Cuts at: 2235 2235 Size: 8602 NarI GG'CG_CC Cuts at: 2231 2231 Size: 8602 NcoI C'CATG_G Cuts at: 513 1065 2004 4437 513 Size: 552 939 2433 4678 Fragments arranged by size: 4678 2433 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8602 NgoMIV G'CCGG_C Cuts at: 2233 2233 Size: 8602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8602 NotI GC'GGCC_GC Cuts at: 6049 6049 Size: 8602 NspI r_CATG'y Cuts at: 1132 1229 3188 4063 4662 4901 5528 5960 7505 Size: 97 1959 875 599 239 627 432 1545 Cuts at: 7505 1132 Size: 2229 Fragments arranged by size: 2229 1959 1545 875 627 599 432 239 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8602 PciI A'CATG_T Cuts at: 7501 7501 Size: 8602 PflMI CCAn_nnn'nTGG Cuts at: 1108 3227 5695 1108 Size: 2119 2468 4015 Fragments arranged by size: 4015 2468 2119 PmeI GTTT'AAAC Cuts at: 5906 5906 Size: 8602 PmlI CAC'GTG Cuts at: 3592 3592 Size: 8602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6671 6703 8184 8216 6671 Size: 32 1481 32 7057 Fragments arranged by size: 7057 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2755 2787 4248 2755 Size: 32 1461 7109 Fragments arranged by size: 7109 1461 32 PshAI GACnn'nnGTC Cuts at: 1613 4972 1613 Size: 3359 5243 PsiI TTA'TAA Cuts at: 5399 6172 5399 Size: 773 7829 PstI C_TGCA'G Cuts at: 830 3336 4265 4709 5163 5954 830 Size: 2506 929 444 454 791 3478 Fragments arranged by size: 3478 2506 929 791 454 444 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8602 PvuII CAG'CTG Cuts at: 2019 3565 4972 5179 2019 Size: 1546 1407 207 5442 Fragments arranged by size: 5442 1546 1407 207 RsrII CG'GwC_CG Cuts at: 2738 2738 Size: 8602 SacI G_AGCT'C Cuts at: 4022 4022 Size: 8602 SacII CC_GC'GG Cuts at: 5820 5820 Size: 8602 SalI G'TCGA_C Cuts at: 1936 4773 5944 1936 Size: 2837 1171 4594 Fragments arranged by size: 4594 2837 1171 SanDI GG'GwC_CC Cuts at: 2787 2787 Size: 8602 SapI GCTCTTCn'nnn_ Cuts at: 3083 5073 3083 Size: 1990 6612 SbfI CC_TGCA'GG Cuts at: 5954 5954 Size: 8602 ScaI AGT'ACT Cuts at: 6850 8562 6850 Size: 1712 6890 SfcI C'TryA_G Cuts at: 826 1045 3332 4261 4705 5159 5552 5950 7085 Size: 219 2287 929 444 454 393 398 1135 Cuts at: 7085 7766 7957 826 Size: 681 191 1471 Fragments arranged by size: 2287 1471 1135 929 681 454 444 398 393 219 191 SfoI GGC'GCC Cuts at: 2232 2232 Size: 8602 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8602 SgrAI Cr'CCGG_yG Cuts at: 1700 2233 2963 1700 Size: 533 730 7339 Fragments arranged by size: 7339 730 533 SmaI CCC'GGG Cuts at: 1872 2089 2785 5929 1872 Size: 217 696 3144 4545 Fragments arranged by size: 4545 3144 696 217 SmlI C'TyrA_G Cuts at: 820 1017 1943 2659 3730 6706 7607 7869 820 Size: 197 926 716 1071 2976 901 262 1553 Fragments arranged by size: 2976 1553 1071 926 901 716 262 197 SnaBI TAC'GTA Cuts at: 493 6393 493 Size: 5900 2702 SpeI A'CTAG_T Cuts at: 152 2318 152 Size: 2166 6436 SphI G_CATG'C Cuts at: 1132 4901 5528 5960 1132 Size: 3769 627 432 3774 Fragments arranged by size: 3774 3769 627 432 SspI AAT'ATT Cuts at: 5 52 5425 6526 5 Size: 47 5373 1101 2081 Fragments arranged by size: 5373 2081 1101 47 StuI AGG'CCT Cuts at: 1517 3311 3368 5693 1517 Size: 1794 57 2325 4426 Fragments arranged by size: 4426 2325 1794 57 StyI C'CwwG_G Cuts at: 513 1065 2004 2722 2836 4437 5694 6041 513 Size: 552 939 718 114 1601 1257 347 3074 Fragments arranged by size: 3074 1601 1257 939 718 552 347 114 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2846 2931 3046 3954 6629 6646 6799 Size: 1255 1266 85 115 908 2675 17 153 Cuts at: 6799 6984 7429 325 Size: 185 445 1498 Fragments arranged by size: 2675 1498 1266 1255 908 445 185 153 115 85 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 3629 4062 Size: 274 80 33 51 1443 642 1010 433 Cuts at: 4062 4951 5136 6848 8560 96 Size: 889 185 1712 1712 138 Fragments arranged by size: 1712 1712 1443 1010 889 642 433 274 185 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8562 8594 8562 Size: 32 8570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8602 XbaI T'CTAG_A Cuts at: 5938 5938 Size: 8602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3274 3274 Size: 8602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8602 XmaI C'CCGG_G Cuts at: 1870 2087 2783 5927 1870 Size: 217 696 3144 4545 Fragments arranged by size: 4545 3144 696 217 XmnI GAAnn'nnTTC Cuts at: 3498 4737 4761 6731 3498 Size: 1239 24 1970 5369 Fragments arranged by size: 5369 1970 1239 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6410 276 Size: 53 83 186 936 4876 2468 Fragments arranged by size: 4876 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstXI BstZ17I DraIII FseI FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI SexAI SfiI SrfI SwaI