Restriction map for
pFN21AA0600
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI BamHI BbrPI BclI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SbfI ScaI
SexAI SfuI SgrAI SnaBI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1179 GTCGAC GT^[AC][GT]AC
AcsI 565 GAATTC [GA]^AATT[CT]
AocI 1645 CCTGAGG CC^T[ACGT]AGG
ApoI 565 GAATTC [GA]^AATT[CT]
AviII 2147 TGCGCA TGC^GCA
AvrII 1119 CCTAGG C^CTAGG
BbeI 1929 GGCGCC GGCGC^C
BcgI 1827 CGAGAGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1873 GGATAC GTATCC(6/5)
BlnI 1119 CCTAGG C^CTAGG
BlpI 820 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 820 GCTGAGC GC^T[ACGT]AGC
BsaI 2980 GAGACC GGTCTC(1/5)
BspHI 1250 TCATGA T^CATGA
BssSI 2593 CACGAG CACGAG(-5/-1)
Bsu36I 1645 CCTGAGG CC^T[ACGT]AGG
CelII 820 GCTGAGC GC^T[ACGT]AGC
DraIII 2112 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2020 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1645 CCTGAGG CC^T[ACGT]AGG
EcoRI 565 GAATTC G^AATTC
FspI 2147 TGCGCA TGC^GCA
HincII 1179 GTCGAC GT[CT]^[GA]AC
HindII 1179 GTCGAC GT[CT]^[GA]AC
KasI 1929 GGCGCC G^GCGCC
NaeI 3075 GCCGGC GCC^GGC
NarI 1929 GGCGCC GG^CGCC
NgoMIV 3075 GCCGGC G^CCGGC
PpuMI 2333 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2648 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1250 TCATGA T^CATGA
SalI 1179 GTCGAC G^TCGAC
SfiI 3030 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1929 GGCGCC GGC^GCC
SpeI 1410 ACTAGT A^CTAGT
Tsp509I 566 AATT ^AATT
XcmI 2537 CCATTGGAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 62 CGCG CG^CG
1463 CGCG
AflIII 1091 ACACGT A^C[GA][CT]GT
1425 ACGTGT
AlwI 1599 GATCC GGATC(4/5)
2901 GATCC
ApaI 730 GGGCCC GGGCC^C
1846 GGGCCC
AvaI 1715 CCCGGG C^[CT]CG[GA]G
3056 CCCGGG
BbsI 1750 GAAGAC GAAGAC(2/6)
1774 GAAGAC
BpuAI 1750 GAAGAC GAAGAC(2/6)
1774 GAAGAC
BsgI 73 CTGCAC GTGCAG(16/14)
2926 GTGCAG
BsoBI 1715 CCCGGG C^[CT]CG[GA]G
3056 CCCGGG
BspMI 1917 GCAGGT ACCTGC(4/8)
2974 GCAGGT
BsrBI 684 GAGCGG CCGCTC(-3/-3)
823 GAGCGG
BstUI 62 CGCG CG^CG
1463 CGCG
BstYI 1598 AGATCC [GA]^GATC[CT]
2900 AGATCC
KspI 61 CCGCGG CCGC^GG
1462 CCGCGG
MflI 1598 AGATCC [GA]^GATC[CT]
2900 AGATCC
MseI 878 TTAA T^TAA
1811 TTAA
MvnI 62 CGCG CG^CG
1463 CGCG
PleI 16 GAGTC GAGTC(4/5)
1509 GAGTC
SacI 235 GAGCTC GAGCT^C
2200 GAGCTC
SacII 61 CCGCGG CCGC^GG
1462 CCGCGG
SmaI 1715 CCCGGG CCC^GGG
3056 CCCGGG
SphI 1320 GCATGC GCATG^C
1439 GCATGC
TfiI 2251 GAATC G^A[AT]TC
2267 GATTC
Tru9I 878 TTAA T^TAA
1811 TTAA
XhoII 1598 AGATCC [GA]^GATC[CT]
2900 AGATCC
XmaCI 1715 CCCGGG C^CCGGG
3056 CCCGGG
XmaI 1715 CCCGGG C^CCGGG
3056 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I