(Circular) (Six-base) MAPSORT of: pFN21AA0600.seq Check: 9300 from: 1 to: 7816 pFN21AA0600 7816 bp With 182 enzymes: SgfI * January 16, 2009 15:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3913 4970 3913 Size: 1057 6759 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5626 278 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 5159 1937 Size: 1248 1974 4594 Fragments arranged by size: 4594 1974 1248 Acc65I G'GTAC_C Cuts at: 5137 5137 Size: 7816 AclI AA'CG_TT Cuts at: 1563 5943 6316 1563 Size: 4380 373 3063 Fragments arranged by size: 4380 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4491 4555 5879 7263 7531 1632 Size: 2859 64 1324 1384 268 1917 Fragments arranged by size: 2859 1917 1384 1324 268 64 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7619 AflIII A'CryG_T Cuts at: 1203 3096 3430 6715 7692 1203 Size: 1893 334 3285 977 1327 Fragments arranged by size: 3285 1893 1327 977 334 AgeI A'CCGG_T Cuts at: 6647 6647 Size: 7816 AhdI GACnn_n'nnGTC Cuts at: 6545 6545 Size: 7816 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7816 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4183 5124 1184 Size: 2999 941 3876 Fragments arranged by size: 3876 2999 941 AlwNI CAG_nnn'CTG Cuts at: 2230 2269 2284 2425 2482 2494 2780 3223 3526 Size: 39 15 141 57 12 286 443 303 Cuts at: 3526 3535 3565 3910 3970 4460 5559 7131 2230 Size: 9 30 345 60 490 1099 1572 2915 Fragments arranged by size: 2915 1572 1099 490 443 345 303 286 141 60 57 39 30 15 12 9 ApaI G_GGCC'C Cuts at: 2739 3855 2739 Size: 1116 6700 ApaLI G'TGCA_C Cuts at: 5873 7029 5873 Size: 1156 6660 ApoI r'AATT_y Cuts at: 1493 2570 5125 5351 5503 7612 1493 Size: 1077 2555 226 152 2109 1697 Fragments arranged by size: 2555 2109 1697 1077 226 152 AseI AT'TA_AT Cuts at: 160 1032 6370 160 Size: 872 5338 1606 Fragments arranged by size: 5338 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 AvaI C'yCGr_G Cuts at: 1870 1943 3720 5061 5141 1870 Size: 73 1777 1341 80 4545 Fragments arranged by size: 4545 1777 1341 80 73 AvrII C'CTAG_G Cuts at: 3124 3124 Size: 7816 BamHI G'GATC_C Cuts at: 5146 5513 5146 Size: 367 7449 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2909 3934 4376 5137 6592 Size: 325 217 627 1122 1025 442 761 1455 Cuts at: 6592 618 Size: 1842 Fragments arranged by size: 1842 1455 1122 1025 761 627 442 325 217 BanII G_rGCy'C Cuts at: 1391 2191 2201 2244 2598 2653 2739 3058 3317 Size: 800 10 43 354 55 86 319 259 Cuts at: 3317 3709 3731 3855 4019 4209 4468 5094 5135 Size: 392 22 124 164 190 259 626 41 Cuts at: 5135 1391 Size: 4072 Fragments arranged by size: 4072 800 626 392 354 319 259 259 190 164 124 86 55 43 41 22 10 BbeI G_GCGC'C Cuts at: 3938 3938 Size: 7816 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3762 3786 928 Size: 971 1863 24 4958 Fragments arranged by size: 4958 1863 971 24 BbvCI CC'TCA_GC Cuts at: 2558 3045 3843 3941 2558 Size: 487 798 98 6433 Fragments arranged by size: 6433 798 487 98 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3821 3855 6007 6041 1903 Size: 34 1884 34 2152 34 3678 Fragments arranged by size: 3678 2152 1884 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3872 5708 6918 3872 Size: 1836 1210 4770 Fragments arranged by size: 4770 1836 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7816 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3288 4796 5042 Size: 107 122 71 631 605 1616 1508 246 Cuts at: 5042 6427 136 Size: 1385 1525 Fragments arranged by size: 1616 1525 1508 1385 631 605 246 122 107 71 BglII A'GATC_T Cuts at: 7811 7811 Size: 7816 BlpI GC'TnA_GC Cuts at: 2826 5233 2826 Size: 2407 5409 Bme1580I G_kGCm'C Cuts at: 1790 2739 3855 3960 5877 7033 1790 Size: 949 1116 105 1917 1156 2573 Fragments arranged by size: 2573 1917 1156 1116 949 105 BmgBI CAC'GTC Cuts at: 1318 3099 4552 1318 Size: 1781 1453 4582 Fragments arranged by size: 4582 1781 1453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2127 3976 4363 Size: 416 53 83 399 24 710 1849 387 Cuts at: 4363 5547 6505 442 Size: 1184 958 1753 Fragments arranged by size: 1849 1753 1184 958 710 416 399 387 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7816 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2697 3507 4153 2697 Size: 810 646 6360 Fragments arranged by size: 6360 810 646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 2489 2504 2549 3200 3320 3377 3587 Size: 1208 42 15 45 651 120 57 210 Cuts at: 3587 3782 4126 4196 4775 4942 6476 1239 Size: 195 344 70 579 167 1534 2579 Fragments arranged by size: 2579 1534 1208 651 579 344 210 195 167 120 70 57 45 42 15 Bpu10I CC'TnA_GC Cuts at: 2558 3045 3482 3843 3941 2558 Size: 487 437 361 98 6433 Fragments arranged by size: 6433 487 437 361 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 2339 4193 5941 6806 7104 2237 Size: 102 1854 1748 865 298 2949 Fragments arranged by size: 2949 1854 1748 865 298 102 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4979 6479 882 Size: 617 3480 1500 2219 Fragments arranged by size: 3480 2219 1500 617 BsaAI yAC'GTr Cuts at: 493 1204 5607 493 Size: 711 4403 2702 Fragments arranged by size: 4403 2702 711 BsaBI GATnn'nnATC Cuts at: 7810 7810 Size: 7816 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2705 2924 3935 5623 Size: 53 83 186 936 1172 219 1011 1688 Cuts at: 5623 6005 6640 275 Size: 382 635 1451 Fragments arranged by size: 1688 1451 1172 1011 936 635 382 219 186 83 53 BsaWI w'CCGG_w Cuts at: 6249 6647 6921 7068 7479 6249 Size: 398 274 147 411 6586 Fragments arranged by size: 6586 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2824 2854 1178 Size: 30 165 30 1421 30 6140 Fragments arranged by size: 6140 1421 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3668 3677 3680 3779 3782 3806 3809 3812 Size: 2482 9 3 99 3 24 3 3 Cuts at: 3812 3839 3860 3878 4129 4653 5006 5102 1186 Size: 27 21 18 251 524 353 96 3900 Fragments arranged by size: 3900 2482 524 353 251 99 96 27 24 21 18 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2165 2643 2960 3286 3595 3694 3903 4380 7019 Size: 478 317 326 309 99 209 477 2639 Cuts at: 7019 7515 2165 Size: 496 2466 Fragments arranged by size: 2639 2466 496 478 477 326 317 309 209 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 4952 1163 Size: 573 327 2889 4027 Fragments arranged by size: 4027 2889 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5266 6027 7055 1540 Size: 275 186 3265 761 1028 2301 Fragments arranged by size: 3265 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 3263 3380 3749 4209 4413 5877 5962 7033 Size: 1019 117 369 460 204 1464 85 1071 Cuts at: 7033 2244 Size: 3027 Fragments arranged by size: 3027 1464 1071 1019 460 369 204 117 85 BsmI GAATG_Cn' Cuts at: 5327 5420 5327 Size: 93 7723 BsmBI CGTCTCn'nnnn_ Cuts at: 2745 3105 4542 2745 Size: 360 1437 6019 Fragments arranged by size: 6019 1437 360 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2191 2201 2244 2598 2653 2739 3058 Size: 399 401 10 43 354 55 86 319 Cuts at: 3058 3263 3317 3380 3709 3731 3749 3855 3960 Size: 205 54 63 329 22 18 106 105 Cuts at: 3960 4019 4209 4413 4468 5094 5135 5877 5962 Size: 59 190 204 55 626 41 742 85 Cuts at: 5962 7033 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 626 401 399 354 329 319 205 204 190 106 105 86 85 63 59 55 55 54 43 41 22 18 10 BspEI T'CCGG_A Cuts at: 7479 7479 Size: 7816 BspHI T'CATG_A Cuts at: 3255 5705 7435 3255 Size: 2450 1730 3636 Fragments arranged by size: 3636 2450 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3913 4970 5171 844 Size: 3069 1057 201 3489 Fragments arranged by size: 3489 3069 1057 201 BsrBI CCG'CTC Cuts at: 1394 1823 2691 2830 5703 1394 Size: 429 868 139 2873 3507 Fragments arranged by size: 3507 2873 868 429 139 BsrDI GCAATG_nn' Cuts at: 65 1248 2535 3384 4378 4590 6311 6485 65 Size: 1183 1287 849 994 212 1721 174 1396 Fragments arranged by size: 1721 1396 1287 1183 994 849 212 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2110 3245 4956 5080 6460 6647 1700 Size: 110 300 1135 1711 124 1380 187 2869 Fragments arranged by size: 2869 1711 1380 1135 300 187 124 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7816 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7816 BssSI C'ACGA_G Cuts at: 4598 5876 6888 4598 Size: 1278 1012 5526 Fragments arranged by size: 5526 1278 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2230 2269 2371 2398 2494 3346 3919 794 Size: 1436 39 102 27 96 852 573 4691 Fragments arranged by size: 4691 1436 852 573 102 96 39 27 BstBI TT'CG_AA Cuts at: 6620 6620 Size: 7816 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7816 BstXI CCAn_nnnn'nTGG Cuts at: 2140 2173 4267 4373 2140 Size: 33 2094 106 5583 Fragments arranged by size: 5583 2094 106 33 BstYI r'GATC_y Cuts at: 1972 3603 4905 5146 5513 5898 5915 7367 7453 Size: 1631 1302 241 367 385 17 1452 86 Cuts at: 7453 7465 7811 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1631 1452 1302 385 367 346 241 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3651 3651 Size: 7816 BtgI C'CryG_G Cuts at: 513 1065 2066 2139 3138 3319 3400 3467 4266 Size: 552 1001 73 999 181 81 67 799 Cuts at: 4266 4366 5013 5094 513 Size: 100 647 81 3235 Fragments arranged by size: 3235 1001 999 799 647 552 181 100 81 81 73 67 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2974 4412 487 Size: 683 56 1748 1438 3891 Fragments arranged by size: 3891 1748 1438 683 56 BtsI GCAGTG_nn' Cuts at: 2213 3165 4167 4884 5332 6126 6146 2213 Size: 952 1002 717 448 794 20 3883 Fragments arranged by size: 3883 1002 952 794 717 448 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7816 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7781 DraI TTT'AAA Cuts at: 5120 5120 Size: 7816 DraIII CAC_nnn'GTG Cuts at: 4122 4122 Size: 7816 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4031 6823 809 Size: 507 2715 2792 1802 Fragments arranged by size: 2792 2715 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3435 3948 4699 Size: 54 1425 50 275 18 1605 513 751 Cuts at: 4699 4881 5263 6152 7781 8 Size: 182 382 889 1629 43 Fragments arranged by size: 1629 1605 1425 889 751 513 382 275 182 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5263 1537 Size: 275 3451 4090 Fragments arranged by size: 4090 3451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2457 2513 2882 4007 4049 4467 5746 1353 Size: 1104 56 369 1125 42 418 1279 3423 Fragments arranged by size: 3423 1279 1125 1104 418 369 56 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4618 6403 6777 6923 387 Size: 1064 138 3029 1785 374 146 1280 Fragments arranged by size: 3029 1785 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 4207 2242 Size: 1965 5851 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 2489 2504 2549 3200 3320 3377 Size: 393 815 42 15 45 651 120 57 Cuts at: 3377 3587 3782 4126 4196 4491 4555 4775 4942 Size: 210 195 344 70 295 64 220 167 Cuts at: 4942 5879 6476 7263 7531 1239 Size: 937 597 787 268 1524 Fragments arranged by size: 1524 937 815 787 651 597 393 344 295 268 220 210 195 167 120 70 64 57 45 42 15 EcoNI CCTnn'n_nnAGG Cuts at: 3227 3764 4967 3227 Size: 537 1203 6076 Fragments arranged by size: 6076 1203 537 EcoO109I rG'GnC_Cy Cuts at: 2736 3211 3523 3851 3967 4055 4339 2736 Size: 475 312 328 116 88 284 6213 Fragments arranged by size: 6213 475 328 312 284 116 88 EcoRI G'AATT_C Cuts at: 2570 5125 5503 2570 Size: 2555 378 4883 Fragments arranged by size: 4883 2555 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4060 4060 Size: 7816 FspI TGC'GCA Cuts at: 4154 6322 4154 Size: 2168 5648 FspAI rTGC'GCAy Cuts at: 4154 4154 Size: 7816 HaeII r_GCGC'y Cuts at: 1678 2252 3938 5013 6963 1678 Size: 574 1686 1075 1950 2531 Fragments arranged by size: 2531 1950 1686 1075 574 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2018 2023 2050 2055 2824 Size: 32 135 32 446 5 27 5 769 Cuts at: 2824 2856 3028 3060 4209 4241 6457 6489 6531 Size: 32 172 32 1149 32 2216 32 42 Cuts at: 6531 6563 1373 Size: 32 2626 Fragments arranged by size: 2626 2216 1149 769 446 172 135 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3186 5160 5406 669 Size: 988 281 1248 1974 246 3079 Fragments arranged by size: 3079 1974 1248 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7816 HpaI GTT'AAC Cuts at: 5406 5406 Size: 7816 KasI G'GCGC_C Cuts at: 3934 3934 Size: 7816 KpnI G_GTAC'C Cuts at: 5141 5141 Size: 7816 MfeI C'AATT_G Cuts at: 5415 5415 Size: 7816 MluI A'CGCG_T Cuts at: 7692 7692 Size: 7816 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2004 6930 7114 1305 Size: 699 4926 184 2007 Fragments arranged by size: 4926 2007 699 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3437 3950 4701 4883 10 Size: 54 1425 343 1605 513 751 182 2943 Fragments arranged by size: 2943 1605 1425 751 513 343 182 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 3035 3324 3389 3443 4103 4160 Size: 648 924 945 289 65 54 660 57 Cuts at: 4160 4265 4371 4431 5774 6133 6292 518 Size: 105 106 60 1343 359 159 2042 Fragments arranged by size: 2042 1343 945 924 660 648 359 289 159 106 105 65 60 57 54 MspA1I CmG'CkG Cuts at: 1606 2068 2269 2341 2437 2443 2455 2479 2494 Size: 462 201 72 96 6 12 24 15 Cuts at: 2494 3469 3505 3535 3595 3910 4717 4946 5232 Size: 975 36 30 60 315 807 229 286 Cuts at: 5232 5909 7057 7302 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 975 807 677 462 315 286 245 229 201 96 72 60 36 30 24 15 12 6 NaeI GCC'GGC Cuts at: 5082 5082 Size: 7816 NarI GG'CG_CC Cuts at: 3935 3935 Size: 7816 NcoI C'CATG_G Cuts at: 513 1065 2139 4266 4366 5013 5094 513 Size: 552 1074 2127 100 647 81 3235 Fragments arranged by size: 3235 2127 1074 647 552 100 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 7816 NgoMIV G'CCGG_C Cuts at: 5080 5080 Size: 7816 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7816 NotI GC'GGCC_GC Cuts at: 5263 5263 Size: 7816 NspI r_CATG'y Cuts at: 1132 1229 3329 3347 3448 5174 6719 1132 Size: 97 2100 18 101 1726 1545 2229 Fragments arranged by size: 2229 2100 1726 1545 101 97 18 PasI CC'CwG_GG Cuts at: 1348 2699 3970 4001 1348 Size: 1351 1271 31 5163 Fragments arranged by size: 5163 1351 1271 31 PciI A'CATG_T Cuts at: 6715 6715 Size: 7816 PflMI CCAn_nnn'nTGG Cuts at: 1108 2643 3970 4460 1108 Size: 1535 1327 490 4464 Fragments arranged by size: 4464 1535 1327 490 PfoI T'CCnGG_A Cuts at: 2565 3564 2565 Size: 999 6817 PmeI GTTT'AAAC Cuts at: 5120 5120 Size: 7816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5885 5917 7398 7430 5885 Size: 32 1481 32 6271 Fragments arranged by size: 6271 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4339 4339 Size: 7816 PshAI GACnn'nnGTC Cuts at: 1613 4657 1613 Size: 3044 4772 PsiI TTA'TAA Cuts at: 5386 5386 Size: 7816 PspOMI G'GGCC_C Cuts at: 2735 3851 2735 Size: 1116 6700 PstI C_TGCA'G Cuts at: 830 2235 2274 2346 3135 3342 3531 3915 4201 Size: 1405 39 72 789 207 189 384 286 Cuts at: 4201 4833 5168 830 Size: 632 335 3478 Fragments arranged by size: 3478 1405 789 632 384 335 286 207 189 72 39 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7816 PvuII CAG'CTG Cuts at: 2269 2341 2494 3535 3595 4717 2269 Size: 72 153 1041 60 1122 5368 Fragments arranged by size: 5368 1122 1041 153 72 60 SacI G_AGCT'C Cuts at: 2244 4209 2244 Size: 1965 5851 SacII CC_GC'GG Cuts at: 2069 3470 2069 Size: 1401 6415 SalI G'TCGA_C Cuts at: 1936 3184 5158 1936 Size: 1248 1974 4594 Fragments arranged by size: 4594 1974 1248 SapI GCTCTTCn'nnn_ Cuts at: 2457 2513 4007 2457 Size: 56 1494 6266 Fragments arranged by size: 6266 1494 56 SbfI CC_TGCA'GG Cuts at: 5168 5168 Size: 7816 ScaI AGT'ACT Cuts at: 6064 7776 6064 Size: 1712 6104 SfcI C'TryA_G Cuts at: 826 1045 2231 2270 2342 3131 3338 3527 3590 Size: 219 1186 39 72 789 207 189 63 Cuts at: 3590 3911 4197 4290 4313 4829 5164 6299 6980 Size: 321 286 93 23 516 335 1135 681 Cuts at: 6980 7171 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 789 681 516 335 321 286 219 207 191 189 93 72 63 39 23 SfiI GGCCn_nnn'nGGCC Cuts at: 5042 5042 Size: 7816 SfoI GGC'GCC Cuts at: 3936 3936 Size: 7816 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7816 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7816 SmaI CCC'GGG Cuts at: 1872 3722 5063 5143 1872 Size: 1850 1341 80 4545 Fragments arranged by size: 4545 1850 1341 80 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2354 4208 5920 6821 7083 Size: 197 926 309 102 1854 1712 901 262 Cuts at: 7083 820 Size: 1553 Fragments arranged by size: 1854 1712 1553 926 901 309 262 197 102 SnaBI TAC'GTA Cuts at: 493 5607 493 Size: 5114 2702 SpeI A'CTAG_T Cuts at: 152 3415 152 Size: 3263 4553 SphI G_CATG'C Cuts at: 1132 3329 3448 5174 1132 Size: 2197 119 1726 3774 Fragments arranged by size: 3774 2197 1726 119 SspI AAT'ATT Cuts at: 5 52 5740 5 Size: 47 5688 2081 Fragments arranged by size: 5688 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7816 StyI C'CwwG_G Cuts at: 513 1065 2052 2139 3124 4266 4366 4836 5013 Size: 552 987 87 985 1142 100 470 177 Cuts at: 5013 5094 5255 513 Size: 81 161 3074 Fragments arranged by size: 3074 1142 987 985 552 470 177 161 100 87 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3659 4394 4658 5003 5843 5860 6013 Size: 1255 2079 735 264 345 840 17 153 Cuts at: 6013 6198 6643 325 Size: 185 445 1498 Fragments arranged by size: 2079 1498 1255 840 735 445 345 264 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6062 7774 96 Size: 274 80 33 51 1443 4085 1712 138 Fragments arranged by size: 4085 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3392 3424 4479 4511 4513 4545 7776 7808 3392 Size: 32 1055 32 2 32 3231 32 3400 Fragments arranged by size: 3400 3231 1055 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7816 XbaI T'CTAG_A Cuts at: 5152 5152 Size: 7816 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4549 4549 Size: 7816 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7816 XmaI C'CCGG_G Cuts at: 1870 3720 5061 5141 1870 Size: 1850 1341 80 4545 Fragments arranged by size: 4545 1850 1341 80 XmnI GAAnn'nnTTC Cuts at: 5945 5945 Size: 7816 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5624 276 Size: 53 83 186 936 4090 2468 Fragments arranged by size: 4090 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FseI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI