Restriction map for
pFN21AA0400
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# NO CUT ENZYMES ( 86 enzymes) #
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AclI AflII AflIII AgeI AhdI
ApaI AscI AseI AsnI Asp700I
AspEI AvaI AviII BciVI BfrI
BglI BlpI Bpu1102I BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FseI FspI
HpaI KspI MfeI MluI MunI
NdeI NheI NotI NruI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1300 GACGTC GACGT^C
Acc65I 1018 GGTACC G^GTACC
AccIII 1378 TCCGGA T^CCGGA
Alw44I 3001 GTGCAC G^TGCAC
Aor51HI 2850 AGCGCT AGC^GCT
ApaLI 3001 GTGCAC G^TGCAC
Asp718I 1018 GGTACC G^GTACC
AspI 1525 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2806 CCTAGG C^CTAGG
BamHI 953 GGATCC G^GATCC
BbrPI 202 CACGTG CAC^GTG
BbvCI 1519 GCTGAGG CCTCAGC(-5/-2)
BcgI 1551 CGACATGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2807 CTAG C^TAG
BglII 11 AGATCT A^GATCT
BlnI 2806 CCTAGG C^CTAGG
BsaAI 202 CACGTG [CT]AC^GT[GA]
BsaBI 2959 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 37 GAGACC GGTCTC(1/5)
BseAI 1378 TCCGGA T^CCGGA
BspEI 1378 TCCGGA T^CCGGA
BssSI 1996 CACGAG CACGAG(-5/-1)
BstAPI 2227 GCAAACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1197 TTTAAA TTT^AAA
DraIII 2406 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2850 AGCGCT AGC^GCT
EcoNI 2232 CCTTGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 25 GAATTC G^AATTC
EcoT22I 1205 ATGCAT ATGCA^T
HgaI 2556 GACGC GACGC(5/10)
KpnI 1018 GGTACC GGTAC^C
MaeI 2807 CTAG C^TAG
MamI 2959 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1378 TCCGGA T^CCGGA
MslI 2953 CACATTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2447 GCCGGC GCC^GGC
NgoMIV 2447 GCCGGC G^CCGGC
NsiI 1205 ATGCAT ATGCA^T
PflFI 1525 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 228 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 202 CACGTG CAC^GTG
PmlI 202 CACGTG CAC^GTG
SacI 2884 GAGCTC GAGCT^C
SapI 214 GAAGAGC GCTCTTC(1/4)
SspI 1471 AATATT AAT^ATT
StuI 1931 AGGCCT AGG^CCT
Tth111I 1525 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 228 CCAGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 744 GTCTAC GT^[AC][GT]AC
1810 GTAGAC
AcsI 25 GAATTC [GA]^AATT[CT]
857 AAATTC
AocI 892 CCTCAGG CC^T[ACGT]AGG
1078 CCTGAGG
ApoI 25 GAATTC [GA]^AATT[CT]
857 AAATTC
BbeI 57 GGCGCC GGCGC^C
1320 GGCGCC
BclI 659 TGATCA T^GATCA
2909 TGATCA
BcnI 2680 CCGGG CC^[GC]GG
2988 CCCGG
BsaWI 140 ACCGGA [AT]^CCGG[AT]
1378 TCCGGA
BsrBI 539 GAGCGG CCGCTC(-3/-3)
919 GAGCGG
Bsu36I 892 CCTCAGG CC^T[ACGT]AGG
1078 CCTGAGG
Eco81I 892 CCTCAGG CC^T[ACGT]AGG
1078 CCTGAGG
FbaI 659 TGATCA T^GATCA
2909 TGATCA
HincII 321 GTTGAC GT[CT]^[GA]AC
613 GTCAAC
HindII 321 GTTGAC GT[CT]^[GA]AC
613 GTCAAC
KasI 57 GGCGCC G^GCGCC
1320 GGCGCC
NarI 57 GGCGCC GG^CGCC
1320 GGCGCC
NciI 2680 CCGGG CC^[GC]GG
2988 CCCGG
NcoI 510 CCATGG C^CATGG
1014 CCATGG
PstI 2327 CTGCAG CTGCA^G
2785 CTGCAG
SfoI 57 GGCGCC GGC^GCC
1320 GGCGCC
XcmI 1324 CCAGATCCTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2022 CCAAGCCTTATCTGG
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# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
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AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbsI BbvI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII