(Circular) (Six-base) MAPSORT of: pFN21AA0400.seq Check: 5428 from: 1 to: 7723 pFN21AA0400 7723 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3309 5533 278 Size: 53 83 186 936 1773 2224 2468 Fragments arranged by size: 2468 2224 1773 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 3816 5066 1937 Size: 813 1066 1250 4594 Fragments arranged by size: 4594 1250 1066 813 Acc65I G'GTAC_C Cuts at: 3023 5044 3023 Size: 2021 5702 AclI AA'CG_TT Cuts at: 1563 5850 6223 1563 Size: 4287 373 3063 Fragments arranged by size: 4287 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2321 2417 2633 2639 3162 4181 5786 7170 Size: 689 96 216 6 523 1019 1605 1384 Cuts at: 7170 7438 1632 Size: 268 1917 Fragments arranged by size: 1917 1605 1384 1019 689 523 268 216 96 6 AfeI AGC'GCT Cuts at: 4857 4857 Size: 7723 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7526 AflIII A'CryG_T Cuts at: 1203 6622 7599 1203 Size: 5419 977 1327 Fragments arranged by size: 5419 1327 977 AgeI A'CCGG_T Cuts at: 6554 6554 Size: 7723 AhdI GACnn_n'nnGTC Cuts at: 6452 6452 Size: 7723 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7723 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3893 4815 1184 Size: 2709 922 4092 Fragments arranged by size: 4092 2709 922 AlwNI CAG_nnn'CTG Cuts at: 2239 2662 3430 5466 7038 2239 Size: 423 768 2036 1572 2924 Fragments arranged by size: 2924 2036 1572 768 423 ApaLI G'TGCA_C Cuts at: 5006 5780 6936 5006 Size: 774 1156 5793 Fragments arranged by size: 5793 1156 774 ApoI r'AATT_y Cuts at: 1493 2030 2862 5032 5258 5410 7519 1493 Size: 537 832 2170 226 152 2109 1697 Fragments arranged by size: 2170 2109 1697 832 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 6277 160 Size: 872 5245 1606 Fragments arranged by size: 5245 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 AvaI C'yCGr_G Cuts at: 1870 1943 5048 1870 Size: 73 3105 4545 Fragments arranged by size: 4545 3105 73 AvrII C'CTAG_G Cuts at: 4811 4811 Size: 7723 BamHI G'GATC_C Cuts at: 2958 5053 5420 2958 Size: 2095 367 5261 Fragments arranged by size: 5261 2095 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 3023 3325 5044 6499 Size: 325 217 627 275 961 302 1719 1455 Cuts at: 6499 618 Size: 1842 Fragments arranged by size: 1842 1719 1455 961 627 325 302 275 217 BanII G_rGCy'C Cuts at: 1391 4187 4300 4893 5042 1391 Size: 2796 113 593 149 4072 Fragments arranged by size: 4072 2796 593 149 113 BbeI G_GCGC'C Cuts at: 2066 3329 2066 Size: 1263 6460 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3080 4098 4161 4427 928 Size: 971 1181 1018 63 266 4224 Fragments arranged by size: 4224 1181 1018 971 266 63 BbvCI CC'TCA_GC Cuts at: 3525 3525 Size: 7723 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3545 3579 5914 5948 1903 Size: 34 50 34 1524 34 2335 34 3678 Fragments arranged by size: 3678 2335 1524 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5615 6825 5615 Size: 1210 6513 BclI T'GATC_A Cuts at: 1548 2664 4914 1548 Size: 1116 2250 4357 Fragments arranged by size: 4357 2250 1116 BfrBI ATG'CAT Cuts at: 3212 3212 Size: 7723 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6334 136 Size: 107 122 71 631 605 4662 1525 Fragments arranged by size: 4662 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2016 7718 2016 Size: 5702 2021 BlpI GC'TnA_GC Cuts at: 5140 5140 Size: 7723 Bme1580I G_kGCm'C Cuts at: 1790 5010 5784 6940 1790 Size: 3220 774 1156 2573 Fragments arranged by size: 3220 2573 1156 774 BmgBI CAC'GTC Cuts at: 1318 2140 1318 Size: 822 6901 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3455 4131 5454 Size: 416 53 83 399 24 2038 676 1323 Cuts at: 5454 6412 442 Size: 958 1753 Fragments arranged by size: 2038 1753 1323 958 676 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3292 3292 Size: 7723 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2059 2261 3255 6383 1239 Size: 820 202 994 3128 2579 Fragments arranged by size: 3128 2579 994 820 202 Bpu10I CC'TnA_GC Cuts at: 3525 4842 3525 Size: 1317 6406 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3899 3980 4603 5848 6713 7011 3899 Size: 81 623 1245 865 298 4611 Fragments arranged by size: 4611 1245 865 623 298 81 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 6386 882 Size: 617 537 4350 2219 Fragments arranged by size: 4350 2219 617 537 BsaAI yAC'GTr Cuts at: 493 1204 2209 5514 493 Size: 711 1005 3305 2702 Fragments arranged by size: 3305 2702 1005 711 BsaBI GATnn'nnATC Cuts at: 4968 7717 4968 Size: 2749 4974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 3306 3326 4562 Size: 53 83 186 936 530 1243 20 1236 Cuts at: 4562 5530 5912 6547 275 Size: 968 382 635 1451 Fragments arranged by size: 1451 1243 1236 968 936 635 530 382 186 83 53 20 BsaWI w'CCGG_w Cuts at: 2145 3383 6156 6554 6828 6975 7386 2145 Size: 1238 2773 398 274 147 411 2482 Fragments arranged by size: 2773 2482 1238 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2003 2033 2357 2387 3080 Size: 30 165 30 600 30 324 30 693 Cuts at: 3080 3110 1178 Size: 30 5791 Fragments arranged by size: 5791 693 600 324 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 3101 3616 4028 4390 4946 1186 Size: 955 960 515 412 362 556 3963 Fragments arranged by size: 3963 960 955 556 515 412 362 BseYI C'CCAG_C Cuts at: 3400 4180 4268 4326 4340 4565 4655 6926 7422 Size: 780 88 58 14 225 90 2271 496 Cuts at: 7422 3400 Size: 3701 Fragments arranged by size: 3701 2271 780 496 225 90 88 58 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3305 3863 4785 1163 Size: 573 1569 558 922 4101 Fragments arranged by size: 4101 1569 922 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5173 5934 6962 1540 Size: 275 186 3172 761 1028 2301 Fragments arranged by size: 3172 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2876 4008 4893 5010 5784 5869 6940 2876 Size: 1132 885 117 774 85 1071 3659 Fragments arranged by size: 3659 1132 1071 885 774 117 85 BsmI GAATG_Cn' Cuts at: 3490 3568 4988 5234 5327 3490 Size: 78 1420 246 93 5886 Fragments arranged by size: 5886 1420 246 93 78 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2876 4008 4187 4300 4893 5010 5042 Size: 399 1086 1132 179 113 593 117 32 Cuts at: 5042 5784 5869 6940 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1132 1086 1071 742 593 399 179 117 113 85 32 BspEI T'CCGG_A Cuts at: 3383 7386 3383 Size: 4003 3720 BspHI T'CATG_A Cuts at: 5612 7342 5612 Size: 1730 5993 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2827 3063 3071 3377 5078 844 Size: 1983 236 8 306 1701 3489 Fragments arranged by size: 3489 1983 1701 306 236 8 BsrBI CCG'CTC Cuts at: 1394 1823 2546 2926 5610 1394 Size: 429 723 380 2684 3507 Fragments arranged by size: 3507 2684 723 429 380 BsrDI GCAATG_nn' Cuts at: 65 1248 2831 3307 3981 6218 6392 65 Size: 1183 1583 476 674 2237 174 1396 Fragments arranged by size: 2237 1583 1396 1183 674 476 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3033 4146 4158 4452 6367 6554 1700 Size: 110 1223 1113 12 294 1915 187 2869 Fragments arranged by size: 2869 1915 1223 1113 294 187 110 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7723 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7723 BssSI C'ACGA_G Cuts at: 4001 5783 6795 4001 Size: 1782 1012 4929 Fragments arranged by size: 4929 1782 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4238 794 Size: 3444 4279 BstBI TT'CG_AA Cuts at: 6527 6527 Size: 7723 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7723 BstXI CCAn_nnnn'nTGG Cuts at: 3355 4573 4783 4964 3355 Size: 1218 210 181 6114 Fragments arranged by size: 6114 1218 210 181 BstYI r'GATC_y Cuts at: 1972 2016 2425 2755 2958 3331 3715 3785 4471 Size: 44 409 330 203 373 384 70 686 Cuts at: 4471 4614 4741 4969 5053 5420 5805 5822 7274 Size: 143 127 228 84 367 385 17 1452 Cuts at: 7274 7360 7372 7718 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 686 409 385 384 373 367 346 330 228 203 143 127 86 84 70 44 17 12 Bsu36I CC'TnA_GG Cuts at: 2898 3084 2898 Size: 186 7537 BtgI C'CryG_G Cuts at: 513 1065 2067 2515 2715 3019 3789 4370 513 Size: 552 1002 448 200 304 770 581 3866 Fragments arranged by size: 3866 1002 770 581 552 448 304 200 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6984 Fragments arranged by size: 6984 683 56 BtsI GCAGTG_nn' Cuts at: 2105 5239 6033 6053 2105 Size: 3134 794 20 3775 Fragments arranged by size: 3775 3134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7723 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7688 DraI TTT'AAA Cuts at: 3204 5027 3204 Size: 1823 5900 DraIII CAC_nnn'GTG Cuts at: 4416 4416 Size: 7723 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6730 809 Size: 507 5414 1802 Fragments arranged by size: 5414 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2118 3738 Size: 54 1425 50 275 18 207 81 1620 Cuts at: 3738 4218 4954 5170 6059 7688 8 Size: 480 736 216 889 1629 43 Fragments arranged by size: 1629 1620 1425 889 736 480 275 216 207 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5170 1537 Size: 275 3358 4090 Fragments arranged by size: 4090 3358 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2214 2553 2942 3810 4509 5653 1353 Size: 861 339 389 868 699 1144 3423 Fragments arranged by size: 3423 1144 868 861 699 389 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4464 6310 6684 6830 387 Size: 1064 138 2875 1846 374 146 1280 Fragments arranged by size: 2875 1846 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4891 4891 Size: 7723 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2059 2261 2321 2417 2633 2639 3162 Size: 393 427 202 60 96 216 6 523 Cuts at: 3162 3255 4181 5786 6383 7170 7438 1239 Size: 93 926 1605 597 787 268 1524 Fragments arranged by size: 1605 1524 926 787 597 523 427 393 268 216 202 96 93 60 6 EcoNI CCTnn'n_nnAGG Cuts at: 4241 4241 Size: 7723 EcoO109I rG'GnC_Cy Cuts at: 2404 4629 4733 4766 2404 Size: 2225 104 33 5361 Fragments arranged by size: 5361 2225 104 33 EcoRI G'AATT_C Cuts at: 2030 5032 5410 2030 Size: 3002 378 4343 Fragments arranged by size: 4343 3002 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3115 3115 Size: 7723 FspI TGC'GCA Cuts at: 6229 6229 Size: 7723 HaeII r_GCGC'y Cuts at: 1678 2066 2581 3329 3994 4356 4859 6870 1678 Size: 388 515 748 665 362 503 2011 2531 Fragments arranged by size: 2531 2011 748 665 515 503 388 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 3785 3817 6364 Size: 32 135 32 431 32 1750 32 2547 Cuts at: 6364 6396 6438 6470 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2547 1750 431 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2328 2620 5067 5313 669 Size: 988 281 390 292 2447 246 3079 Fragments arranged by size: 3079 2447 988 390 292 281 246 HindIII A'AGCT_T Cuts at: 748 2448 3198 3641 3696 748 Size: 1700 750 443 55 4775 Fragments arranged by size: 4775 1700 750 443 55 HpaI GTT'AAC Cuts at: 5313 5313 Size: 7723 KasI G'GCGC_C Cuts at: 2062 3325 2062 Size: 1263 6460 KpnI G_GTAC'C Cuts at: 3027 5048 3027 Size: 2021 5702 MfeI C'AATT_G Cuts at: 5322 5322 Size: 7723 MluI A'CGCG_T Cuts at: 7599 7599 Size: 7723 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 4212 6837 7021 1305 Size: 702 2205 2625 184 2007 Fragments arranged by size: 2625 2205 2007 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3740 4220 4956 10 Size: 54 1425 343 1908 480 736 2777 Fragments arranged by size: 2777 1908 1425 736 480 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4962 5681 6040 6199 518 Size: 648 3796 719 359 159 2042 Fragments arranged by size: 3796 2042 719 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3524 3976 4180 4481 4495 5139 5816 6964 Size: 1918 452 204 301 14 644 677 1148 Cuts at: 6964 7209 1606 Size: 245 2120 Fragments arranged by size: 2120 1918 1148 677 644 452 301 245 204 14 NaeI GCC'GGC Cuts at: 4454 4454 Size: 7723 NarI GG'CG_CC Cuts at: 2063 3326 2063 Size: 1263 6460 NcoI C'CATG_G Cuts at: 513 1065 2515 3019 513 Size: 552 1450 504 5217 Fragments arranged by size: 5217 1450 552 504 NdeI CA'TA_TG Cuts at: 387 387 Size: 7723 NgoMIV G'CCGG_C Cuts at: 4452 4452 Size: 7723 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7723 NotI GC'GGCC_GC Cuts at: 5170 5170 Size: 7723 NsiI A_TGCA'T Cuts at: 3214 3214 Size: 7723 NspI r_CATG'y Cuts at: 1132 1229 5081 6626 1132 Size: 97 3852 1545 2229 Fragments arranged by size: 3852 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7723 PciI A'CATG_T Cuts at: 6622 6622 Size: 7723 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 1108 Size: 1131 6592 PfoI T'CCnGG_A Cuts at: 3416 3416 Size: 7723 PmeI GTTT'AAAC Cuts at: 5027 5027 Size: 7723 PmlI CAC'GTG Cuts at: 2209 2209 Size: 7723 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5792 5824 7305 7337 5792 Size: 32 1481 32 6178 Fragments arranged by size: 6178 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2404 4733 4766 2404 Size: 2329 33 5361 Fragments arranged by size: 5361 2329 33 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7723 PsiI TTA'TAA Cuts at: 5293 5293 Size: 7723 PstI C_TGCA'G Cuts at: 830 4336 4794 5075 830 Size: 3506 458 281 3478 Fragments arranged by size: 3506 3478 458 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7723 PvuII CAG'CTG Cuts at: 3524 4180 4481 3524 Size: 656 301 6766 Fragments arranged by size: 6766 656 301 SacI G_AGCT'C Cuts at: 4893 4893 Size: 7723 SalI G'TCGA_C Cuts at: 1936 5065 1936 Size: 3129 4594 SapI GCTCTTCn'nnn_ Cuts at: 2214 2214 Size: 7723 SbfI CC_TGCA'GG Cuts at: 5075 5075 Size: 7723 ScaI AGT'ACT Cuts at: 5971 7683 5971 Size: 1712 6011 SfcI C'TryA_G Cuts at: 826 1045 2591 4332 4790 5071 6206 6887 7078 Size: 219 1546 1741 458 281 1135 681 191 Cuts at: 7078 826 Size: 1471 Fragments arranged by size: 1741 1546 1471 1135 681 458 281 219 191 SfoI GGC'GCC Cuts at: 2064 3327 2064 Size: 1263 6460 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7723 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7723 SmaI CCC'GGG Cuts at: 1872 5050 1872 Size: 3178 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3914 3995 4582 5827 6728 6990 Size: 197 926 1971 81 587 1245 901 262 Cuts at: 6990 820 Size: 1553 Fragments arranged by size: 1971 1553 1245 926 901 587 262 197 81 SnaBI TAC'GTA Cuts at: 493 5514 493 Size: 5021 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7723 SphI G_CATG'C Cuts at: 1132 5081 1132 Size: 3949 3774 SspI AAT'ATT Cuts at: 5 52 3478 5647 5 Size: 47 3426 2169 2081 Fragments arranged by size: 3426 2169 2081 47 StuI AGG'CCT Cuts at: 1517 3938 1517 Size: 2421 5302 StyI C'CwwG_G Cuts at: 513 1065 2515 3019 4242 4811 5162 513 Size: 552 1450 504 1223 569 351 3074 Fragments arranged by size: 3074 1450 1223 569 552 504 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2246 2936 3911 4819 5750 5767 5920 Size: 1255 666 690 975 908 931 17 153 Cuts at: 5920 6105 6550 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 975 931 908 690 666 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 3442 3591 Size: 274 80 33 51 1443 249 1216 149 Cuts at: 3591 5969 7681 96 Size: 2378 1712 138 Fragments arranged by size: 2378 1712 1443 1216 274 249 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3080 3112 4928 4960 7683 7715 3080 Size: 32 1816 32 2723 32 3088 Fragments arranged by size: 3088 2723 1816 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3533 1316 Size: 2217 5506 XbaI T'CTAG_A Cuts at: 5059 5059 Size: 7723 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3336 4034 3336 Size: 698 7025 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7723 XmaI C'CCGG_G Cuts at: 1870 5048 1870 Size: 3178 4545 XmnI GAAnn'nnTTC Cuts at: 5852 5852 Size: 7723 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3307 5531 276 Size: 53 83 186 936 1773 2224 2468 Fragments arranged by size: 2468 2224 1773 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI