Restriction map for
pFN21AA0400
(Linear) (Six-base) MAPSORT of: KIAA0400.seq Check: 1164 from: 1 to: 3018
KIAA0400.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AatII G_ACGT'C
Cuts at: 0 1304 3018
Size: 1304 1714
AccI GT'mk_AC
Cuts at: 0 745 1811 3018
Size: 745 1066 1207
Fragments arranged by size:
1207 1066 745
Acc65I G'GTAC_C
Cuts at: 0 1018 3018
Size: 1018 2000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 412 628 634 1157 2176 3018
Size: 316 96 216 6 523 1019 842
Fragments arranged by size:
1019 842 523 316 216 96 6
AfeI AGC'GCT
Cuts at: 0 2852 3018
Size: 2852 166
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1888 2810 3018
Size: 1888 922 208
Fragments arranged by size:
1888 922 208
AlwNI CAG_nnn'CTG
Cuts at: 0 234 657 1425 3018
Size: 234 423 768 1593
Fragments arranged by size:
1593 768 423 234
ApaLI G'TGCA_C
Cuts at: 0 3001 3018
Size: 3001 17
ApoI r'AATT_y
Cuts at: 0 25 857 3018
Size: 25 832 2161
Fragments arranged by size:
2161 832 25
AvrII C'CTAG_G
Cuts at: 0 2806 3018
Size: 2806 212
BamHI G'GATC_C
Cuts at: 0 953 3018
Size: 953 2065
BanI G'GyrC_C
Cuts at: 0 57 1018 1320 3018
Size: 57 961 302 1698
Fragments arranged by size:
1698 961 302 57
BanII G_rGCy'C
Cuts at: 0 2182 2295 2888 3018
Size: 2182 113 593 130
Fragments arranged by size:
2182 593 130 113
BbeI G_GCGC'C
Cuts at: 0 61 1324 3018
Size: 61 1263 1694
Fragments arranged by size:
1694 1263 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 1075 2093 2156 2422 3018
Size: 1075 1018 63 266 596
Fragments arranged by size:
1075 1018 596 266 63
BbvCI CC'TCA_GC
Cuts at: 0 1520 3018
Size: 1520 1498
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1540 1574 3018
Size: 1540 34 1444
Fragments arranged by size:
1540 1444 34
BclI T'GATC_A
Cuts at: 0 659 2909 3018
Size: 659 2250 109
Fragments arranged by size:
2250 659 109
BfrBI ATG'CAT
Cuts at: 0 1207 3018
Size: 1207 1811
BglII A'GATC_T
Cuts at: 0 11 3018
Size: 11 3007
Bme1580I G_kGCm'C
Cuts at: 0 3005 3018
Size: 3005 13
BmgBI CAC'GTC
Cuts at: 0 135 3018
Size: 135 2883
BmrI ACTGGGnnnn_n'
Cuts at: 0 1450 2126 3018
Size: 1450 676 892
Fragments arranged by size:
1450 892 676
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 3018
Size: 1287 1731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 256 1250 3018
Size: 54 202 994 1768
Fragments arranged by size:
1768 994 202 54
Bpu10I CC'TnA_GC
Cuts at: 0 1520 2837 3018
Size: 1520 1317 181
Fragments arranged by size:
1520 1317 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 1975 2598 3018
Size: 1894 81 623 420
Fragments arranged by size:
1894 623 420 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 31 3018
Size: 31 2987
BsaAI yAC'GTr
Cuts at: 0 204 3018
Size: 204 2814
BsaBI GATnn'nnATC
Cuts at: 0 2963 3018
Size: 2963 55
BsaHI Gr'CG_yC
Cuts at: 0 58 1301 1321 2557 3018
Size: 58 1243 20 1236 461
Fragments arranged by size:
1243 1236 461 58 20
BsaWI w'CCGG_w
Cuts at: 0 140 1378 3018
Size: 140 1238 1640
Fragments arranged by size:
1640 1238 140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 352 382 1075 1105 3018
Size: 28 324 30 693 30 1913
Fragments arranged by size:
1913 693 324 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 1096 1611 2023 2385 2941 3018
Size: 136 960 515 412 362 556 77
Fragments arranged by size:
960 556 515 412 362 136 77
BseYI C'CCAG_C
Cuts at: 0 1395 2175 2263 2321 2335 2560 2650 3018
Size: 1395 780 88 58 14 225 90 368
Fragments arranged by size:
1395 780 368 225 90 88 58 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 1858 2780 3018
Size: 1300 558 922 238
Fragments arranged by size:
1300 922 558 238
BsiHKAI G_wGCw'C
Cuts at: 0 871 2003 2888 3005 3018
Size: 871 1132 885 117 13
Fragments arranged by size:
1132 885 871 117 13
BsmI GAATG_Cn'
Cuts at: 0 1485 1563 2983 3018
Size: 1485 78 1420 35
Fragments arranged by size:
1485 1420 78 35
Bsp1286I G_dGCh'C
Cuts at: 0 871 2003 2182 2295 2888 3005 3018
Size: 871 1132 179 113 593 117 13
Fragments arranged by size:
1132 871 593 179 117 113 13
BspEI T'CCGG_A
Cuts at: 0 1378 3018
Size: 1378 1640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 822 1058 1066 1372 3018
Size: 822 236 8 306 1646
Fragments arranged by size:
1646 822 306 236 8
BsrBI CCG'CTC
Cuts at: 0 541 921 3018
Size: 541 380 2097
Fragments arranged by size:
2097 541 380
BsrDI GCAATG_nn'
Cuts at: 0 826 1302 1976 3018
Size: 826 476 674 1042
Fragments arranged by size:
1042 826 674 476
BsrFI r'CCGG_y
Cuts at: 0 1028 2141 2153 2447 3018
Size: 1028 1113 12 294 571
Fragments arranged by size:
1113 1028 571 294 12
BssSI C'ACGA_G
Cuts at: 0 1996 3018
Size: 1996 1022
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2233 3018
Size: 2233 785
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1350 2568 2778 2959 3018
Size: 1350 1218 210 181 59
Fragments arranged by size:
1350 1218 210 181 59
BstYI r'GATC_y
Cuts at: 0 11 420 750 953 1326 1710 1780 2466
Size: 11 409 330 203 373 384 70 686
Cuts at: 2466 2609 2736 2964 3018
Size: 143 127 228 54
Fragments arranged by size:
686 409 384 373 330 228 203 143
127 70 54 11
Bsu36I CC'TnA_GG
Cuts at: 0 893 1079 3018
Size: 893 186 1939
Fragments arranged by size:
1939 893 186
BtgI C'CryG_G
Cuts at: 0 62 510 710 1014 1784 2365 3018
Size: 62 448 200 304 770 581 653
Fragments arranged by size:
770 653 581 448 304 200 62
BtsI GCAGTG_nn'
Cuts at: 0 100 3018
Size: 100 2918
DraI TTT'AAA
Cuts at: 0 1199 3018
Size: 1199 1819
DraIII CAC_nnn'GTG
Cuts at: 0 2411 3018
Size: 2411 607
EaeI y'GGCC_r
Cuts at: 0 32 113 1733 2213 2949 3018
Size: 32 81 1620 480 736 69
Fragments arranged by size:
1620 736 480 81 69 32
EarI CTCTTCn'nnn_
Cuts at: 0 209 548 937 1805 2504 3018
Size: 209 339 389 868 699 514
Fragments arranged by size:
868 699 514 389 339 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2459 3018
Size: 2459 559
EcoICRI GAG'CTC
Cuts at: 0 2886 3018
Size: 2886 132
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 256 316 412 628 634 1157 1250
Size: 54 202 60 96 216 6 523 93
Cuts at: 1250 2176 3018
Size: 926 842
Fragments arranged by size:
926 842 523 216 202 96 93 60
54 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2236 3018
Size: 2236 782
EcoO109I rG'GnC_Cy
Cuts at: 0 399 2624 2728 2761 3018
Size: 399 2225 104 33 257
Fragments arranged by size:
2225 399 257 104 33
EcoRI G'AATT_C
Cuts at: 0 25 3018
Size: 25 2993
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1110 3018
Size: 1110 1908
HaeII r_GCGC'y
Cuts at: 0 61 576 1324 1989 2351 2854 3018
Size: 61 515 748 665 362 503 164
Fragments arranged by size:
748 665 515 503 362 164 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1780 1812 3018
Size: 30 1750 32 1206
Fragments arranged by size:
1750 1206 32 30
HincII GTy'rAC
Cuts at: 0 323 615 3018
Size: 323 292 2403
Fragments arranged by size:
2403 323 292
HindIII A'AGCT_T
Cuts at: 0 443 1193 1636 1691 3018
Size: 443 750 443 55 1327
Fragments arranged by size:
1327 750 443 443 55
KasI G'GCGC_C
Cuts at: 0 57 1320 3018
Size: 57 1263 1698
Fragments arranged by size:
1698 1263 57
KpnI G_GTAC'C
Cuts at: 0 1022 3018
Size: 1022 1996
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 2207 3018
Size: 2 2205 811
Fragments arranged by size:
2205 811 2
MscI TGG'CCA
Cuts at: 0 1735 2215 2951 3018
Size: 1735 480 736 67
Fragments arranged by size:
1735 736 480 67
MslI CAynn'nnrTG
Cuts at: 0 2957 3018
Size: 2957 61
MspA1I CmG'CkG
Cuts at: 0 1519 1971 2175 2476 2490 3018
Size: 1519 452 204 301 14 528
Fragments arranged by size:
1519 528 452 301 204 14
NaeI GCC'GGC
Cuts at: 0 2449 3018
Size: 2449 569
NarI GG'CG_CC
Cuts at: 0 58 1321 3018
Size: 58 1263 1697
Fragments arranged by size:
1697 1263 58
NcoI C'CATG_G
Cuts at: 0 510 1014 3018
Size: 510 504 2004
Fragments arranged by size:
2004 510 504
NgoMIV G'CCGG_C
Cuts at: 0 2447 3018
Size: 2447 571
NsiI A_TGCA'T
Cuts at: 0 1209 3018
Size: 1209 1809
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 3018
Size: 234 2784
PfoI T'CCnGG_A
Cuts at: 0 1411 3018
Size: 1411 1607
PmlI CAC'GTG
Cuts at: 0 204 3018
Size: 204 2814
PpuMI rG'GwC_Cy
Cuts at: 0 399 2728 2761 3018
Size: 399 2329 33 257
Fragments arranged by size:
2329 399 257 33
PstI C_TGCA'G
Cuts at: 0 2331 2789 3018
Size: 2331 458 229
Fragments arranged by size:
2331 458 229
PvuII CAG'CTG
Cuts at: 0 1519 2175 2476 3018
Size: 1519 656 301 542
Fragments arranged by size:
1519 656 542 301
SacI G_AGCT'C
Cuts at: 0 2888 3018
Size: 2888 130
SapI GCTCTTCn'nnn_
Cuts at: 0 209 3018
Size: 209 2809
SfcI C'TryA_G
Cuts at: 0 586 2327 2785 3018
Size: 586 1741 458 233
Fragments arranged by size:
1741 586 458 233
SfoI GGC'GCC
Cuts at: 0 59 1322 3018
Size: 59 1263 1696
Fragments arranged by size:
1696 1263 59
SmlI C'TyrA_G
Cuts at: 0 1909 1990 2577 3018
Size: 1909 81 587 441
Fragments arranged by size:
1909 587 441 81
SspI AAT'ATT
Cuts at: 0 1473 3018
Size: 1473 1545
StuI AGG'CCT
Cuts at: 0 1933 3018
Size: 1933 1085
StyI C'CwwG_G
Cuts at: 0 510 1014 2237 2806 3018
Size: 510 504 1223 569 212
Fragments arranged by size:
1223 569 510 504 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 931 1906 2814 3018
Size: 241 690 975 908 204
Fragments arranged by size:
975 908 690 241 204
TatI w'GTAC_w
Cuts at: 0 221 1437 1586 3018
Size: 221 1216 149 1432
Fragments arranged by size:
1432 1216 221 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1075 1107 2923 2955 3018
Size: 1075 32 1816 32 63
Fragments arranged by size:
1816 1075 63 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1528 3018
Size: 1528 1490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1331 2029 3018
Size: 1331 698 989
Fragments arranged by size:
1331 989 698
ZraI GAC'GTC
Cuts at: 0 1302 3018
Size: 1302 1716
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AlfI AlwNI ApaLI
ApoI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BclI BfrBI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtsI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI PflMI PfoI PmlI
PpuMI PstI PvuII SacI SapI SfcI SfoI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvaI BaeI BciVI BglI
BlpI BmtI BsiEI BsiWI BsmBI BspHI BsrGI BssHII
BstBI BstEII BstZ17I BtgZI ClaI CspCI DrdI EagI
EcoRV FseI FspI FspAI HpaI MfeI MluI NdeI
NheI NotI NruI NspI PacI PasI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI XbaI
XhoI XmaI XmnI