Restriction map for
pFN21AA0196
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BanII BbeI BbrPI BbsI
BcgI BfrI BglI BlnI BpuAI
BsaBI BsaHI BsaWI BseAI BsiHKAI
BsiWI BsmBI BspDI BspEI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoT22I FseI FspI
HgaI Hin1I HpaI HphI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacI SacII SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI XcmI
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 2149 CGCG CG^CG
AclI 2454 AACGTT AA^CGTT
AocI 45 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3101 AGCGCT AGC^GCT
AseI 1798 ATTAAT AT^TAAT
AsnI 1798 ATTAAT AT^TAAT
AspI 2278 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1980 GCTGAGG CCTCAGC(-5/-2)
BmyI 3371 GTGCCC G[AGT]GC[ACT]^C
BsaAI 775 TACGTG [CT]AC^GT[GA]
BsaI 711 GGTCTC GGTCTC(1/5)
BsiEI 3200 CGACCG CG[GA][CT]^CG
Bsp1286I 3371 GTGCCC G[AGT]GC[ACT]^C
BspHI 2735 TCATGA T^CATGA
BspMI 1820 ACCTGC ACCTGC(4/8)
BsrBI 1342 GAGCGG CCGCTC(-3/-3)
BstAPI 928 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 569 TTCGAA TT^CGAA
BstUI 2149 CGCG CG^CG
Bsu36I 45 CCTAAGG CC^T[ACGT]AGG
Eco47III 3101 AGCGCT AGC^GCT
Eco81I 45 CCTAAGG CC^T[ACGT]AGG
EcoRI 1528 GAATTC G^AATTC
MfeI 3119 CAATTG C^AATTG
MunI 3119 CAATTG C^AATTG
MvnI 2149 CGCG CG^CG
NspV 569 TTCGAA TT^CGAA
PflFI 2278 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1798 ATTAAT AT^TAAT
Psp1406I 2454 AACGTT AA^CGTT
RcaI 2735 TCATGA T^CATGA
ScaI 1838 AGTACT AGT^ACT
SfuI 569 TTCGAA TT^CGAA
SphI 1011 GCATGC GCATG^C
SspI 1446 AATATT AAT^ATT
Tth111I 2278 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 336 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 2509 ACATGT A^C[GA][CT]GT
3435 ACATGT
BamHI 1223 GGATCC G^GATCC
2100 GGATCC
BanI 598 GGTGCC G^G[CT][GA]CC
3370 GGTGCC
BcnI 3207 CCCGG CC^[GC]GG
3266 CCCGG
BglII 688 AGATCT A^GATCT
1433 AGATCT
BseRI 1054 GAGGAG GAGGAG(10/8)
1624 GAGGAG
BsgI 1250 CTGCAC GTGCAG(16/14)
1021 GTGCAG
BsmI 2739 GAATGC GAATGC(1/-1)
3062 GCATTC
BspLU11I 2509 ACATGT A^CATGT
3435 ACATGT
BstXI 190 CCAGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
853 CCAGATAATTGG
DraII 1655 GGGACCT [GA]G^G[ACGT]CC[CT]
2987 AGGACCC
EcoO109I 1655 GGGACCT [GA]G^G[ACGT]CC[CT]
2987 AGGACCC
EcoRV 157 GATATC GAT^ATC
332 GATATC
FauI 1961 CCCGC CCCGC(4/6)
1480 GCGGG
HaeII 3101 AGCGCT [GA]GCGC^[CT]
3288 GGCGCT
NciI 3207 CCCGG CC^[GC]GG
3266 CCCGG
NcoI 1730 CCATGG C^CATGG
2240 CCATGG
PaeR7I 2189 CTCGAG C^TCGAG
2833 CTCGAG
PflMI 1115 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1533 CCACCAGTTGG
PpuMI 1655 GGGACCT [GA]G^G[AT]CC[CT]
2987 AGGACCC
PstI 2472 CTGCAG CTGCA^G
3356 CTGCAG
SalI 680 GTCGAC G^TCGAC
2465 GTCGAC
SexAI 594 ACCAGGT A^CC[AT]GGT
707 ACCAGGT
Van91I 1115 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1533 CCACCAGTTGG
XhoI 2189 CTCGAG C^TCGAG
2833 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
AvaII BalI BbvI BciVI BclI
BfaI BlpI BpmI Bpu1102I BsaJI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI