(Circular) (Six-base) MAPSORT of: pFN21AA0196.seq Check: 7015 from: 1 to: 8182 pFN21AA0196 8182 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5992 278 Size: 53 83 186 936 4456 2468 Fragments arranged by size: 4456 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2325 2686 4471 5525 1937 Size: 388 361 1785 1054 4594 Fragments arranged by size: 4594 1785 1054 388 361 Acc65I G'GTAC_C Cuts at: 5503 5503 Size: 8182 AclI AA'CG_TT Cuts at: 1563 4460 6309 6682 1563 Size: 2897 1849 373 3063 Fragments arranged by size: 3063 2897 1849 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 4077 4325 5276 5454 6245 7629 7897 Size: 2288 157 248 951 178 791 1384 268 Cuts at: 7897 1632 Size: 1917 Fragments arranged by size: 2288 1917 1384 951 791 268 248 178 157 AfeI AGC'GCT Cuts at: 5108 5108 Size: 8182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7985 AflIII A'CryG_T Cuts at: 1203 4514 5440 7081 8058 1203 Size: 3311 926 1641 977 1327 Fragments arranged by size: 3311 1641 1327 977 926 AgeI A'CCGG_T Cuts at: 7013 7013 Size: 8182 AhdI GACnn_n'nnGTC Cuts at: 6911 6911 Size: 8182 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8182 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8182 AlwNI CAG_nnn'CTG Cuts at: 3244 4120 4873 5290 5925 7497 3244 Size: 876 753 417 635 1572 3929 Fragments arranged by size: 3929 1572 876 753 635 417 ApaLI G'TGCA_C Cuts at: 6239 7395 6239 Size: 1156 7026 ApoI r'AATT_y Cuts at: 1493 2252 3533 4672 4777 4788 5355 5491 5717 Size: 759 1281 1139 105 11 567 136 226 Cuts at: 5717 5869 7978 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1281 1139 759 567 226 152 136 105 11 AseI AT'TA_AT Cuts at: 160 1032 3804 6736 160 Size: 872 2772 2932 1606 Fragments arranged by size: 2932 2772 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 AvaI C'yCGr_G Cuts at: 1870 1943 2627 4194 4838 5507 1870 Size: 73 684 1567 644 669 4545 Fragments arranged by size: 4545 1567 684 669 644 73 BamHI G'GATC_C Cuts at: 3228 4105 5512 5879 3228 Size: 877 1407 367 5531 Fragments arranged by size: 5531 1407 877 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2603 5375 5503 6958 618 Size: 325 217 627 816 2772 128 1455 1842 Fragments arranged by size: 2772 1842 1455 816 627 325 217 128 BanII G_rGCy'C Cuts at: 1391 5501 1391 Size: 4110 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7211 BbvCI CC'TCA_GC Cuts at: 3986 3986 Size: 8182 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6373 6407 1903 Size: 34 4436 34 3678 Fragments arranged by size: 4436 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2736 3244 3874 6074 7284 2736 Size: 508 630 2200 1210 3634 Fragments arranged by size: 3634 2200 1210 630 508 BclI T'GATC_A Cuts at: 1548 2143 3606 3639 1548 Size: 595 1463 33 6091 Fragments arranged by size: 6091 1463 595 33 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6793 136 Size: 107 122 71 631 605 5121 1525 Fragments arranged by size: 5121 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2693 3438 8177 2693 Size: 745 4739 2698 Fragments arranged by size: 4739 2698 745 BlpI GC'TnA_GC Cuts at: 2223 3830 3999 5599 2223 Size: 1607 169 1600 4806 Fragments arranged by size: 4806 1607 1600 169 Bme1580I G_kGCm'C Cuts at: 1790 5380 6243 7399 1790 Size: 3590 863 1156 2573 Fragments arranged by size: 3590 2573 1156 863 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8182 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5913 6871 442 Size: 416 53 83 399 24 4496 958 1753 Fragments arranged by size: 4496 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3873 4964 5075 5411 6842 1239 Size: 2634 1091 111 336 1431 2579 Fragments arranged by size: 2634 2579 1431 1091 336 111 Bpu10I CC'TnA_GC Cuts at: 3986 3986 Size: 8182 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3006 6307 7172 7470 3006 Size: 3301 865 298 3718 Fragments arranged by size: 3718 3301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2722 6845 882 Size: 617 1223 4123 2219 Fragments arranged by size: 4123 2219 1223 617 BsaAI yAC'GTr Cuts at: 493 1204 2782 5973 493 Size: 711 1578 3191 2702 Fragments arranged by size: 3191 2702 1578 711 BsaBI GATnn'nnATC Cuts at: 8176 8176 Size: 8182 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5989 6371 7006 275 Size: 53 83 186 936 4456 382 635 1451 Fragments arranged by size: 4456 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6615 7013 7287 7434 7845 6615 Size: 398 274 147 411 6952 Fragments arranged by size: 6952 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3846 3876 4343 4373 1178 Size: 30 165 30 2443 30 467 30 4987 Fragments arranged by size: 4987 2443 467 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3074 3644 1186 Size: 1888 570 5724 Fragments arranged by size: 5724 1888 570 BseYI C'CCAG_C Cuts at: 7385 7881 7385 Size: 496 7686 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3047 3240 1163 Size: 573 1311 193 6105 Fragments arranged by size: 6105 1311 573 193 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5208 5632 6393 7421 1540 Size: 275 186 3207 424 761 1028 2301 Fragments arranged by size: 3207 2301 1028 761 424 275 186 BsiHKAI G_wGCw'C Cuts at: 6243 6328 7399 6243 Size: 85 1071 7026 Fragments arranged by size: 7026 1071 85 BsmI GAATG_Cn' Cuts at: 4750 5067 5693 5786 4750 Size: 317 626 93 7146 Fragments arranged by size: 7146 626 317 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 5380 5501 6243 6328 7399 1391 Size: 399 3590 121 742 85 1071 2174 Fragments arranged by size: 3590 2174 1071 742 399 121 85 BspEI T'CCGG_A Cuts at: 7845 7845 Size: 8182 BspHI T'CATG_A Cuts at: 4740 6071 7801 4740 Size: 1331 1730 5121 Fragments arranged by size: 5121 1730 1331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3834 5537 844 Size: 2990 1703 3489 Fragments arranged by size: 3489 2990 1703 BsrBI CCG'CTC Cuts at: 1394 1823 3349 6069 1394 Size: 429 1526 2720 3507 Fragments arranged by size: 3507 2720 1526 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2079 3118 4803 6677 6851 65 Size: 1183 831 1039 1685 1874 174 1396 Fragments arranged by size: 1874 1685 1396 1183 1039 831 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6826 7013 1700 Size: 110 5016 187 2869 Fragments arranged by size: 5016 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2308 3499 5333 96 Size: 2212 1191 1834 2945 Fragments arranged by size: 2945 2212 1834 1191 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8182 BssSI C'ACGA_G Cuts at: 6242 7254 6242 Size: 1012 7170 BstAPI GCAn_nnn'nTGC Cuts at: 794 2939 794 Size: 2145 6037 BstBI TT'CG_AA Cuts at: 2575 6986 2575 Size: 4411 3771 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8182 BstXI CCAn_nnnn'nTGG Cuts at: 2202 2865 2202 Size: 663 7519 BstYI r'GATC_y Cuts at: 1972 2693 3228 3438 4105 5512 5879 6264 6281 Size: 721 535 210 667 1407 367 385 17 Cuts at: 6281 7733 7819 7831 8177 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1407 721 667 535 385 367 346 210 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2051 2051 Size: 8182 BtgI C'CryG_G Cuts at: 513 1065 3735 4245 5319 513 Size: 552 2670 510 1074 3376 Fragments arranged by size: 3376 2670 1074 552 510 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2726 487 Size: 683 56 1500 5943 Fragments arranged by size: 5943 1500 683 56 BtsI GCAGTG_nn' Cuts at: 5336 5698 6492 6512 5336 Size: 362 794 20 7006 Fragments arranged by size: 7006 794 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2017 2052 529 Size: 35 1453 35 6659 Fragments arranged by size: 6659 1453 35 35 DraI TTT'AAA Cuts at: 2946 3462 4731 5486 2946 Size: 516 1269 755 5642 Fragments arranged by size: 5642 1269 755 516 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7189 809 Size: 507 5873 1802 Fragments arranged by size: 5873 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 5222 5302 Size: 54 1425 50 275 18 208 3184 80 Cuts at: 5302 5629 6518 8147 8 Size: 327 889 1629 43 Fragments arranged by size: 3184 1629 1425 889 327 275 208 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5629 1537 Size: 275 3817 4090 Fragments arranged by size: 4090 3817 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3239 3516 3921 4589 6112 1353 Size: 1886 277 405 668 1523 3423 Fragments arranged by size: 3423 1886 1523 668 405 277 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4789 6769 7143 7289 387 Size: 1064 138 3200 1980 374 146 1280 Fragments arranged by size: 3200 1980 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3873 3920 4077 4325 4964 5075 5276 Size: 393 2241 47 157 248 639 111 201 Cuts at: 5276 5411 5454 6245 6842 7629 7897 1239 Size: 135 43 791 597 787 268 1524 Fragments arranged by size: 2241 1524 791 787 639 597 393 268 248 201 157 135 111 47 43 EcoO109I rG'GnC_Cy Cuts at: 3661 4993 3661 Size: 1332 6850 EcoRI G'AATT_C Cuts at: 3533 5491 5869 3533 Size: 1958 378 5846 Fragments arranged by size: 5846 1958 378 EcoRV GAT'ATC Cuts at: 2164 2339 2164 Size: 175 8007 FspI TGC'GCA Cuts at: 6688 6688 Size: 8182 HaeII r_GCGC'y Cuts at: 1678 5110 5297 7329 1678 Size: 3432 187 2032 2531 Fragments arranged by size: 3432 2531 2032 187 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2637 2669 3848 3880 4819 Size: 32 135 32 1065 32 1179 32 939 Cuts at: 4819 4851 6823 6855 6897 6929 1373 Size: 32 1972 32 42 32 2626 Fragments arranged by size: 2626 1972 1179 1065 939 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2687 4291 4472 5526 5772 669 Size: 988 281 749 1604 181 1054 246 3079 Fragments arranged by size: 3079 1604 1054 988 749 281 246 181 HindIII A'AGCT_T Cuts at: 748 3086 3423 3513 3767 3932 4028 748 Size: 2338 337 90 254 165 96 4902 Fragments arranged by size: 4902 2338 337 254 165 96 90 HpaI GTT'AAC Cuts at: 5772 5772 Size: 8182 KpnI G_GTAC'C Cuts at: 5507 5507 Size: 8182 MfeI C'AATT_G Cuts at: 5124 5781 5124 Size: 657 7525 MluI A'CGCG_T Cuts at: 8058 8058 Size: 8182 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2643 3228 3525 7296 7480 1305 Size: 684 654 585 297 3771 184 2007 Fragments arranged by size: 3771 2007 684 654 585 297 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2040 5224 5304 10 Size: 54 1425 343 208 3184 80 2888 Fragments arranged by size: 3184 2888 1425 343 208 80 54 MslI CAynn'nnrTG Cuts at: 518 1166 2672 4067 4100 6140 6499 6658 518 Size: 648 1506 1395 33 2040 359 159 2042 Fragments arranged by size: 2042 2040 1506 1395 648 359 159 33 MspA1I CmG'CkG Cuts at: 1606 3241 4531 4937 5063 5598 6275 7423 7668 Size: 1635 1290 406 126 535 677 1148 245 Cuts at: 7668 1606 Size: 2120 Fragments arranged by size: 2120 1635 1290 1148 677 535 406 245 126 NcoI C'CATG_G Cuts at: 513 1065 3735 4245 513 Size: 552 2670 510 4450 Fragments arranged by size: 4450 2670 552 510 NdeI CA'TA_TG Cuts at: 387 387 Size: 8182 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8182 NotI GC'GGCC_GC Cuts at: 5629 5629 Size: 8182 NspI r_CATG'y Cuts at: 1132 1229 3020 3907 4405 4518 5444 5540 7085 Size: 97 1791 887 498 113 926 96 1545 Cuts at: 7085 1132 Size: 2229 Fragments arranged by size: 2229 1791 1545 926 887 498 113 97 96 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8182 PciI A'CATG_T Cuts at: 4514 5440 7081 4514 Size: 926 1641 5615 Fragments arranged by size: 5615 1641 926 PflMI CCAn_nnn'nTGG Cuts at: 1108 3126 3544 1108 Size: 2018 418 5746 Fragments arranged by size: 5746 2018 418 PfoI T'CCnGG_A Cuts at: 5388 5388 Size: 8182 PmeI GTTT'AAAC Cuts at: 5486 5486 Size: 8182 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6251 6283 7764 7796 6251 Size: 32 1481 32 6637 Fragments arranged by size: 6637 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3661 4993 3661 Size: 1332 6850 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8182 PsiI TTA'TAA Cuts at: 5752 5752 Size: 8182 PspXI vC'TCGA_Gb Cuts at: 4194 4194 Size: 8182 PstI C_TGCA'G Cuts at: 830 4481 5365 5534 830 Size: 3651 884 169 3478 Fragments arranged by size: 3651 3478 884 169 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8182 PvuII CAG'CTG Cuts at: 3241 4531 4937 5063 3241 Size: 1290 406 126 6360 Fragments arranged by size: 6360 1290 406 126 SalI G'TCGA_C Cuts at: 1936 2685 4470 5524 1936 Size: 749 1785 1054 4594 Fragments arranged by size: 4594 1785 1054 749 SbfI CC_TGCA'GG Cuts at: 5534 5534 Size: 8182 ScaI AGT'ACT Cuts at: 3845 6430 8142 3845 Size: 2585 1712 3885 Fragments arranged by size: 3885 2585 1712 SexAI A'CCwGG_T Cuts at: 2599 2712 2599 Size: 113 8069 SfcI C'TryA_G Cuts at: 826 1045 2234 4477 5361 5530 6665 7346 7537 Size: 219 1189 2243 884 169 1135 681 191 Cuts at: 7537 826 Size: 1471 Fragments arranged by size: 2243 1471 1189 1135 884 681 219 191 169 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8182 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8182 SmaI CCC'GGG Cuts at: 1872 5509 1872 Size: 3637 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3021 4194 4838 6286 7187 7449 Size: 197 926 1078 1173 644 1448 901 262 Cuts at: 7449 820 Size: 1553 Fragments arranged by size: 1553 1448 1173 1078 926 901 644 262 197 SnaBI TAC'GTA Cuts at: 493 5973 493 Size: 5480 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8182 SphI G_CATG'C Cuts at: 1132 3020 5540 1132 Size: 1888 2520 3774 Fragments arranged by size: 3774 2520 1888 SspI AAT'ATT Cuts at: 5 52 3453 6106 5 Size: 47 3401 2653 2081 Fragments arranged by size: 3401 2653 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8182 StyI C'CwwG_G Cuts at: 513 1065 3735 4245 4605 5621 513 Size: 552 2670 510 360 1016 3074 Fragments arranged by size: 3074 2670 1016 552 510 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2831 5222 5363 6209 6226 6379 6564 Size: 1255 1251 2391 141 846 17 153 185 Cuts at: 6564 7009 325 Size: 445 1498 Fragments arranged by size: 2391 1498 1255 1251 846 445 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2308 2433 2579 Size: 274 80 33 51 1443 331 125 146 Cuts at: 2579 3499 3843 5333 6428 8140 96 Size: 920 344 1490 1095 1712 138 Fragments arranged by size: 1712 1490 1443 1095 920 344 331 274 146 138 125 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4343 4375 4405 4437 5217 5249 8142 8174 4343 Size: 32 30 32 780 32 2893 32 4351 Fragments arranged by size: 4351 2893 780 32 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 1316 4286 1316 Size: 2970 5212 XbaI T'CTAG_A Cuts at: 2341 5518 2341 Size: 3177 5005 XhoI C'TCGA_G Cuts at: 1943 4194 4838 1943 Size: 2251 644 5287 Fragments arranged by size: 5287 2251 644 XmaI C'CCGG_G Cuts at: 1870 5507 1870 Size: 3637 4545 XmnI GAAnn'nnTTC Cuts at: 2253 3417 3525 5261 6311 2253 Size: 1164 108 1736 1050 4124 Fragments arranged by size: 4124 1736 1164 1050 108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5990 276 Size: 53 83 186 936 4456 2468 Fragments arranged by size: 4456 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI