(Circular) (Six-base) MAPSORT of: pFN21AA0107.seq Check: 8268 from: 1 to: 5872 pFN21AA0107 5872 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3682 278 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3215 1937 Size: 1278 4594 Acc65I G'GTAC_C Cuts at: 3193 3193 Size: 5872 AclI AA'CG_TT Cuts at: 1563 3999 4372 1563 Size: 2436 373 3063 Fragments arranged by size: 3063 2436 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3935 5319 5587 1632 Size: 2303 1384 268 1917 Fragments arranged by size: 2303 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5675 AflIII A'CryG_T Cuts at: 1203 4771 5748 1203 Size: 3568 977 1327 Fragments arranged by size: 3568 1327 977 AgeI A'CCGG_T Cuts at: 4703 4703 Size: 5872 AhdI GACnn_n'nnGTC Cuts at: 4601 4601 Size: 5872 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5872 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5872 AlwNI CAG_nnn'CTG Cuts at: 2782 2926 3004 3615 5187 2782 Size: 144 78 611 1572 3467 Fragments arranged by size: 3467 1572 611 144 78 ApaLI G'TGCA_C Cuts at: 3929 5085 3929 Size: 1156 4716 ApoI r'AATT_y Cuts at: 1493 2301 2904 2987 3181 3407 3559 5668 1493 Size: 808 603 83 194 226 152 2109 1697 Fragments arranged by size: 2109 1697 808 603 226 194 152 83 AseI AT'TA_AT Cuts at: 160 1032 3165 4426 160 Size: 872 2133 1261 1606 Fragments arranged by size: 2133 1606 1261 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3197 1870 Size: 73 147 1107 4545 Fragments arranged by size: 4545 1107 147 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2041 2074 2041 Size: 33 5839 BamHI G'GATC_C Cuts at: 3202 3569 3202 Size: 367 5505 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3193 4648 618 Size: 325 217 627 1406 1455 1842 Fragments arranged by size: 1842 1455 1406 627 325 217 BanII G_rGCy'C Cuts at: 1391 2365 3191 1391 Size: 974 826 4072 Fragments arranged by size: 4072 974 826 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2753 928 Size: 971 854 4047 Fragments arranged by size: 4047 971 854 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2167 2201 4063 4097 1903 Size: 34 50 34 146 34 1862 34 3678 Fragments arranged by size: 3678 1862 146 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3764 4974 3764 Size: 1210 4662 BclI T'GATC_A Cuts at: 1548 2995 1548 Size: 1447 4425 BfrBI ATG'CAT Cuts at: 2912 2912 Size: 5872 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4483 136 Size: 107 122 71 631 605 2811 1525 Fragments arranged by size: 2811 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2707 3124 5867 2707 Size: 417 2743 2712 Fragments arranged by size: 2743 2712 417 BlpI GC'TnA_GC Cuts at: 2081 3289 2081 Size: 1208 4664 Bme1580I G_kGCm'C Cuts at: 1790 3933 5089 1790 Size: 2143 1156 2573 Fragments arranged by size: 2573 2143 1156 BmgBI CAC'GTC Cuts at: 1318 2203 1318 Size: 885 4987 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2533 3603 4561 Size: 416 53 83 399 24 1116 1070 958 Cuts at: 4561 442 Size: 1753 Fragments arranged by size: 1753 1116 1070 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5872 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 2042 4532 1239 Size: 794 9 2490 2579 Fragments arranged by size: 2579 2490 794 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3997 4862 5160 3997 Size: 865 298 4709 Fragments arranged by size: 4709 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4535 882 Size: 617 3036 2219 Fragments arranged by size: 3036 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3663 493 Size: 711 2459 2702 Fragments arranged by size: 2702 2459 711 BsaBI GATnn'nnATC Cuts at: 2466 5866 2466 Size: 3400 2472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3679 4061 4696 275 Size: 53 83 186 936 2146 382 635 1451 Fragments arranged by size: 2146 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4305 4703 4977 5124 5535 4305 Size: 398 274 147 411 4642 Fragments arranged by size: 4642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5647 Fragments arranged by size: 5647 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2276 2532 1186 Size: 853 3 234 256 4526 Fragments arranged by size: 4526 853 256 234 3 BseYI C'CCAG_C Cuts at: 5075 5571 5075 Size: 496 5376 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5299 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 3322 4083 5111 1540 Size: 275 186 135 1186 761 1028 2301 Fragments arranged by size: 2301 1186 1028 761 275 186 135 BsiHKAI G_wGCw'C Cuts at: 2097 2365 3933 4018 5089 2097 Size: 268 1568 85 1071 2880 Fragments arranged by size: 2880 1568 1071 268 85 BsmI GAATG_Cn' Cuts at: 2804 3383 3476 2804 Size: 579 93 5200 Fragments arranged by size: 5200 579 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2097 2097 Size: 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2365 3191 3933 4018 5089 1391 Size: 399 307 268 826 742 85 1071 2174 Fragments arranged by size: 2174 1071 826 742 399 307 268 85 BspEI T'CCGG_A Cuts at: 5535 5535 Size: 5872 BspHI T'CATG_A Cuts at: 3761 5491 3761 Size: 1730 4142 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3227 844 Size: 2383 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3759 1394 Size: 429 1936 3507 Fragments arranged by size: 3507 1936 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2319 4367 4541 65 Size: 1183 1071 2048 174 1396 Fragments arranged by size: 2048 1396 1183 1071 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4516 4703 1700 Size: 110 2706 187 2869 Fragments arranged by size: 2869 2706 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5872 BssSI C'ACGA_G Cuts at: 3932 4944 3932 Size: 1012 4860 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5872 BstBI TT'CG_AA Cuts at: 4676 4676 Size: 5872 BstEII G'GTnAC_C Cuts at: 1184 2408 1184 Size: 1224 4648 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5872 BstYI r'GATC_y Cuts at: 1972 2707 3124 3202 3569 3954 3971 5423 5509 Size: 735 417 78 367 385 17 1452 86 Cuts at: 5509 5521 5867 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 735 417 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2438 2438 Size: 5872 BtgI C'CryG_G Cuts at: 513 1065 2098 513 Size: 552 1033 4287 Fragments arranged by size: 4287 1033 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2322 487 Size: 683 56 1096 4037 Fragments arranged by size: 4037 1096 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3388 4182 4202 3034 Size: 354 794 20 4704 Fragments arranged by size: 4704 794 354 20 ClaI AT'CG_AT Cuts at: 1554 2881 1554 Size: 1327 4545 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5837 DraI TTT'AAA Cuts at: 3176 3176 Size: 5872 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4879 809 Size: 507 3563 1802 Fragments arranged by size: 3563 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3319 4208 Size: 54 1425 50 275 18 303 1186 889 Cuts at: 4208 5837 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1186 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2133 3319 1537 Size: 275 321 1186 4090 Fragments arranged by size: 4090 1186 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2609 3802 1353 Size: 1256 1193 3423 Fragments arranged by size: 3423 1256 1193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4459 4833 4979 387 Size: 1064 138 2870 374 146 1280 Fragments arranged by size: 2870 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2363 2363 Size: 5872 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2042 3935 4532 5319 5587 1239 Size: 393 401 9 1893 597 787 268 1524 Fragments arranged by size: 1893 1524 787 597 401 393 268 9 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2024 Size: 5872 EcoRI G'AATT_C Cuts at: 2904 2987 3181 3559 2904 Size: 83 194 378 5217 Fragments arranged by size: 5217 378 194 83 FspI TGC'GCA Cuts at: 4378 4378 Size: 5872 HaeII r_GCGC'y Cuts at: 1678 5019 1678 Size: 3341 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2694 2726 4513 4545 4587 Size: 32 135 32 1122 32 1787 32 42 Cuts at: 4587 4619 1373 Size: 32 2626 Fragments arranged by size: 2626 1787 1122 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3216 3462 669 Size: 988 281 1278 246 3079 Fragments arranged by size: 3079 1278 988 281 246 HindIII A'AGCT_T Cuts at: 748 2500 748 Size: 1752 4120 HpaI GTT'AAC Cuts at: 3462 3462 Size: 5872 KpnI G_GTAC'C Cuts at: 3197 3197 Size: 5872 MfeI C'AATT_G Cuts at: 3471 3471 Size: 5872 MluI A'CGCG_T Cuts at: 5748 5748 Size: 5872 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4986 5170 1305 Size: 3681 184 2007 Fragments arranged by size: 3681 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4050 Fragments arranged by size: 4050 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3830 4189 4348 518 Size: 648 2664 359 159 2042 Fragments arranged by size: 2664 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2068 2100 2597 2923 3288 3965 5113 Size: 405 57 32 497 326 365 677 1148 Cuts at: 5113 5358 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 497 405 365 326 245 57 32 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5320 NdeI CA'TA_TG Cuts at: 387 2386 387 Size: 1999 3873 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5872 NotI GC'GGCC_GC Cuts at: 3319 3319 Size: 5872 NruI TCG'CGA Cuts at: 2307 2307 Size: 5872 NsiI A_TGCA'T Cuts at: 2914 2914 Size: 5872 NspI r_CATG'y Cuts at: 1132 1229 3230 4775 1132 Size: 97 2001 1545 2229 Fragments arranged by size: 2229 2001 1545 97 PasI CC'CwG_GG Cuts at: 1348 2404 1348 Size: 1056 4816 PciI A'CATG_T Cuts at: 4771 4771 Size: 5872 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5872 PmeI GTTT'AAAC Cuts at: 3176 3176 Size: 5872 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3941 3973 5454 5486 3941 Size: 32 1481 32 4327 Fragments arranged by size: 4327 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5872 PsiI TTA'TAA Cuts at: 2652 3442 2652 Size: 790 5082 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3083 3115 3083 Size: 32 5840 PstI C_TGCA'G Cuts at: 830 3224 830 Size: 2394 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5872 PvuII CAG'CTG Cuts at: 2068 2597 2923 2068 Size: 529 326 5017 Fragments arranged by size: 5017 529 326 SacI G_AGCT'C Cuts at: 2365 2365 Size: 5872 SacII CC_GC'GG Cuts at: 2101 2101 Size: 5872 SalI G'TCGA_C Cuts at: 1936 3214 1936 Size: 1278 4594 SbfI CC_TGCA'GG Cuts at: 3224 3224 Size: 5872 ScaI AGT'ACT Cuts at: 4120 5832 4120 Size: 1712 4160 SfcI C'TryA_G Cuts at: 826 1045 3220 4355 5036 5227 826 Size: 219 2175 1135 681 191 1471 Fragments arranged by size: 2175 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5872 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5872 SmaI CCC'GGG Cuts at: 1872 3199 1872 Size: 1327 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3976 4877 5139 820 Size: 197 926 2033 901 262 1553 Fragments arranged by size: 2033 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3663 493 Size: 3170 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5872 SphI G_CATG'C Cuts at: 1132 3230 1132 Size: 2098 3774 SspI AAT'ATT Cuts at: 5 52 3796 5 Size: 47 3744 2081 Fragments arranged by size: 3744 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5872 StyI C'CwwG_G Cuts at: 513 1065 2950 3311 513 Size: 552 1885 361 3074 Fragments arranged by size: 3074 1885 552 361 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3899 3916 4069 4254 4699 325 Size: 1255 2319 17 153 185 445 1498 Fragments arranged by size: 2319 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4118 5830 96 Size: 274 80 33 51 1443 2141 1712 138 Fragments arranged by size: 2141 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5832 5864 5832 Size: 32 5840 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5872 XbaI T'CTAG_A Cuts at: 3208 3208 Size: 5872 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5872 XmaI C'CCGG_G Cuts at: 1870 3197 1870 Size: 1327 4545 XmnI GAAnn'nnTTC Cuts at: 4001 4001 Size: 5872 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3680 276 Size: 53 83 186 936 2146 2468 Fragments arranged by size: 2468 2146 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI