(Circular) (Six-base) MAPSORT of: pFN21AA0078.seq Check: 2229 from: 1 to: 6598 pFN21AA0078 6598 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3941 1937 Size: 2004 4594 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 4725 5098 1563 Size: 3162 373 3063 Fragments arranged by size: 3162 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2358 3182 3689 4661 6045 6313 1632 Size: 726 824 507 972 1384 268 1917 Fragments arranged by size: 1917 1384 972 824 726 507 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 2547 5497 6474 1203 Size: 1344 2950 977 1327 Fragments arranged by size: 2950 1344 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6598 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6598 AlwNI CAG_nnn'CTG Cuts at: 2365 3464 4341 5913 2365 Size: 1099 877 1572 3050 Fragments arranged by size: 3050 1572 1099 877 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 2052 2342 3248 3260 3907 4133 4285 6394 Size: 559 290 906 12 647 226 152 2109 Cuts at: 6394 1493 Size: 1697 Fragments arranged by size: 2109 1697 906 647 559 290 226 152 12 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 3923 1870 Size: 73 1980 4545 Fragments arranged by size: 4545 1980 73 BamHI G'GATC_C Cuts at: 2848 3928 4295 2848 Size: 1080 367 5151 Fragments arranged by size: 5151 1080 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 3919 5374 618 Size: 325 217 627 1277 855 1455 1842 Fragments arranged by size: 1842 1455 1277 855 627 325 217 BanII G_rGCy'C Cuts at: 1391 3022 3333 3917 1391 Size: 1631 311 584 4072 Fragments arranged by size: 4072 1631 584 311 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 3695 928 Size: 971 1308 488 3831 Fragments arranged by size: 3831 1308 971 488 BbvCI CC'TCA_GC Cuts at: 3477 3477 Size: 6598 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4490 5700 4490 Size: 1210 5388 BclI T'GATC_A Cuts at: 1548 2356 2878 1548 Size: 808 522 5268 Fragments arranged by size: 5268 808 522 BfrBI ATG'CAT Cuts at: 3635 3635 Size: 6598 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5209 136 Size: 107 122 71 631 605 3537 1525 Fragments arranged by size: 3537 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6593 6593 Size: 6598 BlpI GC'TnA_GC Cuts at: 3135 4015 3135 Size: 880 5718 Bme1580I G_kGCm'C Cuts at: 1790 4659 5815 1790 Size: 2869 1156 2573 Fragments arranged by size: 2869 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2195 4329 5287 Size: 416 53 83 399 24 778 2134 958 Cuts at: 5287 442 Size: 1753 Fragments arranged by size: 2134 1753 958 778 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2063 1056 Size: 1007 5591 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3341 3747 5258 1239 Size: 2102 406 1511 2579 Fragments arranged by size: 2579 2102 1511 406 Bpu10I CC'TnA_GC Cuts at: 3477 3477 Size: 6598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3770 4723 5588 5886 3770 Size: 953 865 298 4482 Fragments arranged by size: 4482 953 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5261 882 Size: 617 3762 2219 Fragments arranged by size: 3762 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4389 493 Size: 711 3185 2702 Fragments arranged by size: 3185 2702 711 BsaBI GATnn'nnATC Cuts at: 3651 6592 3651 Size: 2941 3657 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5031 5429 5703 5850 6261 5031 Size: 398 274 147 411 5368 Fragments arranged by size: 5368 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6373 Fragments arranged by size: 6373 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3120 3240 3465 1186 Size: 1934 120 225 4319 Fragments arranged by size: 4319 1934 225 120 BseYI C'CCAG_C Cuts at: 2394 5801 6297 2394 Size: 3407 496 2695 Fragments arranged by size: 3407 2695 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2766 1163 Size: 573 1030 4995 Fragments arranged by size: 4995 1030 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4809 5837 1540 Size: 275 186 2047 761 1028 2301 Fragments arranged by size: 2301 2047 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2600 3715 4659 4744 5815 2600 Size: 1115 944 85 1071 3383 Fragments arranged by size: 3383 1115 1071 944 85 BsmI GAATG_Cn' Cuts at: 2246 4109 4202 2246 Size: 1863 93 4642 Fragments arranged by size: 4642 1863 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2600 3022 3333 3715 3917 4659 4744 Size: 399 810 422 311 382 202 742 85 Cuts at: 4744 5815 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 810 742 422 399 382 311 202 85 BspEI T'CCGG_A Cuts at: 6261 6261 Size: 6598 BspHI T'CATG_A Cuts at: 2347 4487 6217 2347 Size: 2140 1730 2728 Fragments arranged by size: 2728 2140 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 3139 3472 3953 844 Size: 1454 841 333 481 3489 Fragments arranged by size: 3489 1454 841 481 333 BsrBI CCG'CTC Cuts at: 1394 1823 4485 1394 Size: 429 2662 3507 Fragments arranged by size: 3507 2662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5242 5429 1700 Size: 110 3432 187 2869 Fragments arranged by size: 3432 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6598 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 4658 5670 4658 Size: 1012 5586 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6598 BstBI TT'CG_AA Cuts at: 2197 5402 2197 Size: 3205 3393 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstYI r'GATC_y Cuts at: 1972 2848 3058 3928 4295 4680 4697 6149 6235 Size: 876 210 870 367 385 17 1452 86 Cuts at: 6235 6247 6593 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 876 870 385 367 346 210 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2295 3423 2295 Size: 1128 5470 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6046 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3850 487 Size: 683 56 2624 3235 Fragments arranged by size: 3235 2624 683 56 BtsI GCAGTG_nn' Cuts at: 2363 2533 4114 4908 4928 2363 Size: 170 1581 794 20 4033 Fragments arranged by size: 4033 1581 794 170 20 ClaI AT'CG_AT Cuts at: 1554 2385 3324 1554 Size: 831 939 4828 Fragments arranged by size: 4828 939 831 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 3902 3902 Size: 6598 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2273 4045 Size: 54 1425 50 275 18 216 227 1772 Cuts at: 4045 4934 6563 8 Size: 889 1629 43 Fragments arranged by size: 1772 1629 1425 889 275 227 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2421 2960 3336 3609 3615 4528 1353 Size: 1068 539 376 273 6 913 3423 Fragments arranged by size: 3423 1068 913 539 376 273 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5185 5559 5705 387 Size: 1064 138 3596 374 146 1280 Fragments arranged by size: 3596 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2358 3182 3341 3689 3747 4661 5258 Size: 393 726 824 159 348 58 914 597 Cuts at: 5258 6045 6313 1239 Size: 787 268 1524 Fragments arranged by size: 1524 914 824 787 726 597 393 348 268 159 58 EcoNI CCTnn'n_nnAGG Cuts at: 2294 2750 2294 Size: 456 6142 EcoO109I rG'GnC_Cy Cuts at: 2039 2039 Size: 6598 EcoRI G'AATT_C Cuts at: 3248 3907 4285 3248 Size: 659 378 5561 Fragments arranged by size: 5561 659 378 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 5745 1678 Size: 4067 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2798 2830 3460 3492 5239 Size: 32 135 32 1226 32 630 32 1747 Cuts at: 5239 5271 5313 5345 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1747 1226 630 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2287 2983 3942 4188 669 Size: 988 281 349 696 959 246 3079 Fragments arranged by size: 3079 988 959 696 349 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6598 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2979 5712 5896 1305 Size: 1674 2733 184 2007 Fragments arranged by size: 2733 2007 1674 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 10 Size: 54 1425 343 216 4560 Fragments arranged by size: 4560 1425 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2387 4556 4915 5074 518 Size: 648 1221 2169 359 159 2042 Fragments arranged by size: 2169 2042 1221 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4014 4691 5839 6084 1606 Size: 2408 677 1148 245 2120 Fragments arranged by size: 2408 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6046 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NheI G'CTAG_C Cuts at: 1052 2059 1052 Size: 1007 5591 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NsiI A_TGCA'T Cuts at: 3637 3637 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2551 3956 5501 1132 Size: 97 1322 1405 1545 2229 Fragments arranged by size: 2229 1545 1405 1322 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 2547 5497 2547 Size: 2950 3648 PflMI CCAn_nnn'nTGG Cuts at: 1108 2055 1108 Size: 947 5651 PfoI T'CCnGG_A Cuts at: 3357 3357 Size: 6598 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6598 PsiI TTA'TAA Cuts at: 2319 4168 2319 Size: 1849 4749 PstI C_TGCA'G Cuts at: 830 3950 830 Size: 3120 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 3336 3336 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SfcI C'TryA_G Cuts at: 826 1045 3507 3796 3946 5081 5762 5953 826 Size: 219 2462 289 150 1135 681 191 1471 Fragments arranged by size: 2462 1471 1135 681 289 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3925 1872 Size: 2053 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3749 4702 5603 5865 820 Size: 197 926 1806 953 901 262 1553 Fragments arranged by size: 1806 1553 953 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6598 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SspI AAT'ATT Cuts at: 5 52 2688 4522 5 Size: 47 2636 1834 2081 Fragments arranged by size: 2636 2081 1834 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 3881 4037 513 Size: 552 2816 156 3074 Fragments arranged by size: 3074 2816 552 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2810 4625 4642 4795 4980 5425 325 Size: 1255 1230 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 1230 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4844 6556 96 Size: 274 80 33 51 1443 2867 1712 138 Fragments arranged by size: 2867 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6558 6590 6558 Size: 32 6566 Tth111I GACn'n_nGTC Cuts at: 1316 2987 1316 Size: 1671 4927 XbaI T'CTAG_A Cuts at: 3934 3934 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 3923 1870 Size: 2053 4545 XmnI GAAnn'nnTTC Cuts at: 2343 4727 2343 Size: 2384 4214 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI