Restriction map for
pFN21AA0072
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BanI BbeI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiWI
BsmAI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssSI Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PleI PmeI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 946 GTAGAC GT^[AC][GT]AC
AlwNI 631 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 249 CCTCAGG CC^T[ACGT]AGG
Asp700I 226 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1189 GAGCTC G[AT]GC[AT]^C
AvaI 1175 CTCGGG C^[CT]CG[GA]G
BbrPI 235 CACGTG CAC^GTG
BciVI 193 GTATCC GTATCC(6/5)
BcnI 242 CCCGG CC^[GC]GG
BglII 863 AGATCT A^GATCT
BsaAI 235 CACGTG [CT]AC^GT[GA]
BseRI 58 GAGGAG GAGGAG(10/8)
BsiHKAI 1189 GAGCTC G[AT]GC[AT]^C
BsmFI 270 GGGAC GGGAC(10/14)
BsmI 1053 GAATGC GAATGC(1/-1)
BsoBI 1175 CTCGGG C^[CT]CG[GA]G
BspMI 260 ACCTGC ACCTGC(4/8)
BsrDI 1260 CATTGC GCAATG(2/0)
BssHII 68 GCGCGC G^CGCGC
BstAPI 87 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 624 GGTAACC G^GT[ACGT]ACC
BstPI 624 GGTAACC G^GT[ACGT]ACC
Bsu36I 249 CCTCAGG CC^T[ACGT]AGG
DraI 597 TTTAAA TTT^AAA
DraII 920 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 76 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1197 CCGTGG C^C[GA][CT]GG
EaeI 1256 CGGCCA [CT]^GGCC[GA]
Eco81I 249 CCTCAGG CC^T[ACGT]AGG
EcoO109I 920 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 624 GGTAACC G^GT[ACGT]ACC
FauI 141 CCCGC CCCGC(4/6)
HincII 256 GTTGAC GT[CT]^[GA]AC
HindII 256 GTTGAC GT[CT]^[GA]AC
HindIII 1349 AAGCTT A^AGCTT
MaeII 236 ACGT A^CGT
MfeI 1403 CAATTG C^AATTG
MslI 280 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 52 CCGCTG C[AC]G^C[GT]G
MunI 1403 CAATTG C^AATTG
NciI 242 CCCGG CC^[GC]GG
PmaCI 235 CACGTG CAC^GTG
PmlI 235 CACGTG CAC^GTG
PpuMI 920 AGGACCC [GA]G^G[AT]CC[CT]
SacI 1189 GAGCTC GAGCT^C
SmlI 734 CTCAAG C^T[CT][GA]AG
SpeI 1374 ACTAGT A^CTAGT
TaqI 543 TCGA T^CGA
TthHB8I 543 TCGA T^CGA
XcmI 611 CCACAAGTGGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 226 GAACCGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 67 CGCG CG^CG
69 CGCG
AcsI 336 AAATTC [GA]^AATT[CT]
755 AAATTT
AfaI 608 GTAC GT^AC
1107 GTAC
AgeI 228 ACCGGT A^CCGGT
1328 ACCGGT
ApoI 336 AAATTC [GA]^AATT[CT]
755 AAATTT
AvaII 921 GGACC G^G[AT]CC
1326 GGACC
BamHI 967 GGATCC G^GATCC
1179 GGATCC
BanII 1189 GAGCTC G[GA]GC[CT]^C
1277 GGGCTC
BfaI 797 CTAG C^TAG
1375 CTAG
BmyI 1189 GAGCTC G[AGT]GC[ACT]^C
1277 GGGCTC
BsaWI 228 ACCGGT [AT]^CCGG[AT]
1328 ACCGGT
Bsp1286I 1189 GAGCTC G[AGT]GC[ACT]^C
1277 GGGCTC
BsrBI 97 CCGCTC CCGCTC(-3/-3)
142 CCGCTC
BsrFI 228 ACCGGT [GA]^CCGG[CT]
1328 ACCGGT
BstUI 67 CGCG CG^CG
69 CGCG
Cfr10I 228 ACCGGT [GA]^CCGG[CT]
1328 ACCGGT
HaeII 18 GGCGCT [GA]GCGC^[CT]
48 AGCGCC
MaeI 797 CTAG C^TAG
1375 CTAG
MvnI 67 CGCG CG^CG
69 CGCG
PflMI 9 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAAGCTATGG
PinAI 228 ACCGGT A^CCGGT
1328 ACCGGT
PstI 85 CTGCAG CTGCA^G
262 CTGCAG
RsaI 608 GTAC GT^AC
1107 GTAC
SapI 144 GCTCTTC GCTCTTC(1/4)
1191 GCTCTTC
Tsp45I 585 GTCAC ^GT[GC]AC
691 GTGAC
Van91I 9 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAAGCTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI BbvI BpmI
BsaJI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI SfcI TfiI Tru9I
TseI Tsp509I TspRI XhoII