Restriction map for
pFN21AA0059
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# NO CUT ENZYMES ( 101 enzymes) #
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AccII AccIII AclI AcsI AflII
AgeI AhdI Aor51HI ApaI ApoI
AscI AspEI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmI BsoBI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstUI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoT22I FbaI FseI FspI HpaI
KasI KspI MamI MluI MroI
MvnI NarI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI SwaI
TaqI TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 1613 GACGTC GACGT^C
Acc65I 1133 GGTACC G^GTACC
AccI 946 GTAGAC GT^[AC][GT]AC
AcyI 1613 GACGTC G[GA]^CG[CT]C
AflIII 1946 ACATGT A^C[GA][CT]GT
AlwNI 529 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 73 CCTCAGG CC^T[ACGT]AGG
AseI 1638 ATTAAT AT^TAAT
AsnI 1638 ATTAAT AT^TAAT
Asp718I 1133 GGTACC G^GTACC
BalI 1899 TGGCCA TGG^CCA
BbsI 840 GAAGAC GAAGAC(2/6)
BpuAI 840 GAAGAC GAAGAC(2/6)
BsaHI 1613 GACGTC G[GA]^CG[CT]C
BsaI 401 GAGACC GGTCTC(1/5)
BseRI 1928 GAGGAG GAGGAG(10/8)
BsgI 255 CTGCAC GTGCAG(16/14)
BsiEI 1278 CGGCCG CG[GA][CT]^CG
Bsp1407I 1199 TGTACA T^GTACA
BspLU11I 1946 ACATGT A^CATGT
BsrBI 1233 CCGCTC CCGCTC(-3/-3)
BsrGI 1199 TGTACA T^GTACA
BssSI 1170 CACGAG CACGAG(-5/-1)
Bsu36I 73 CCTCAGG CC^T[ACGT]AGG
DraI 571 TTTAAA TTT^AAA
EagI 1278 CGGCCG C^GGCCG
EclXI 1278 CGGCCG C^GGCCG
Eco52I 1278 CGGCCG C^GGCCG
Eco81I 73 CCTCAGG CC^T[ACGT]AGG
EcoNI 2013 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 2120 GACGC GACGC(5/10)
Hin1I 1613 GACGTC G[GA]^CG[CT]C
HincII 437 GTCAAC GT[CT]^[GA]AC
HindII 437 GTCAAC GT[CT]^[GA]AC
HindIII 1645 AAGCTT A^AGCTT
KpnI 1133 GGTACC GGTAC^C
MluNI 1899 TGGCCA TGG^CCA
MscI 1899 TGGCCA TGG^CCA
NaeI 499 GCCGGC GCC^GGC
NdeI 1970 CATATG CA^TATG
NgoMIV 499 GCCGGC G^CCGGC
PflMI 806 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1638 ATTAAT AT^TAAT
PvuII 738 CAGCTG CAG^CTG
SacI 25 GAGCTC GAGCT^C
SapI 1630 GAAGAGC GCTCTTC(1/4)
SexAI 921 ACCAGGT A^CC[AT]GGT
SphI 1410 GCATGC GCATG^C
SspBI 1199 TGTACA T^GTACA
StuI 1656 AGGCCT AGG^CCT
Van91I 806 CCACCGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 651 CCAGGGACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 446 GTGCAC G^TGCAC
743 GTGCAC
ApaLI 446 GTGCAC G^TGCAC
743 GTGCAC
Asp700I 145 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2059 GAAGTGTTTC
AspI 403 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1104 GACTTTGTC
BanI 1133 GGTACC G^G[CT][GA]CC
1482 GGCACC
BanII 25 GAGCTC G[GA]GC[CT]^C
1179 GGGCTC
BfaI 893 CTAG C^TAG
1577 CTAG
BglII 1023 AGATCT A^GATCT
1573 AGATCT
BspMI 190 ACCTGC ACCTGC(4/8)
715 GCAGGT
BsrDI 1262 GCAATG GCAATG(2/0)
1912 GCAATG
BstEII 1986 GGTGACC G^GT[ACGT]ACC
2105 GGTTACC
BstPI 1986 GGTGACC G^GT[ACGT]ACC
2105 GGTTACC
DsaI 47 CCATGG C^C[GA][CT]GG
294 CCATGG
EcoO65I 1986 GGTGACC G^GT[ACGT]ACC
2105 GGTTACC
EcoRV 514 GATATC GAT^ATC
1447 GATATC
MaeI 893 CTAG C^TAG
1577 CTAG
MfeI 357 CAATTG C^AATTG
492 CAATTG
MunI 357 CAATTG C^AATTG
492 CAATTG
NcoI 47 CCATGG C^CATGG
294 CCATGG
PflFI 403 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1104 GACTTTGTC
TfiI 1373 GAATC G^A[AT]TC
1735 GATTC
Tth111I 403 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1104 GACTTTGTC
XmnI 145 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2059 GAAGTGTTTC
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# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BbvI BcnI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI Bsp1286I BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII