Restriction map for
pFN21AA0059
(Linear) (Six-base) MAPSORT of: KIAA0059.seq Check: 4081 from: 1 to: 2154
KIAA0059.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 199 706 2154
Size: 199 507 1448
Fragments arranged by size:
1448 507 199
AatII G_ACGT'C
Cuts at: 0 1617 2154
Size: 1617 537
AccI GT'mk_AC
Cuts at: 0 947 2154
Size: 947 1207
Acc65I G'GTAC_C
Cuts at: 0 1133 2154
Size: 1133 1021
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2078 2154
Size: 2078 76
AflIII A'CryG_T
Cuts at: 0 1946 2154
Size: 1946 208
AlwNI CAG_nnn'CTG
Cuts at: 0 534 2154
Size: 534 1620
ApaLI G'TGCA_C
Cuts at: 0 446 743 2154
Size: 446 297 1411
Fragments arranged by size:
1411 446 297
AseI AT'TA_AT
Cuts at: 0 1639 2154
Size: 1639 515
BanI G'GyrC_C
Cuts at: 0 1133 1482 2154
Size: 1133 349 672
Fragments arranged by size:
1133 672 349
BanII G_rGCy'C
Cuts at: 0 29 1183 2154
Size: 29 1154 971
Fragments arranged by size:
1154 971 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 847 2154
Size: 847 1307
BglII A'GATC_T
Cuts at: 0 1023 1573 2154
Size: 1023 550 581
Fragments arranged by size:
1023 581 550
Bme1580I G_kGCm'C
Cuts at: 0 450 747 2154
Size: 450 297 1407
Fragments arranged by size:
1407 450 297
BmgBI CAC'GTC
Cuts at: 0 1229 2154
Size: 1229 925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 268 2154
Size: 268 1886
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 730 888 2059 2154
Size: 471 259 158 1171 95
Fragments arranged by size:
1171 471 259 158 95
Bpu10I CC'TnA_GC
Cuts at: 0 1337 2154
Size: 1337 817
BsaI GGTCTCn'nnnn_
Cuts at: 0 395 2154
Size: 395 1759
BsaHI Gr'CG_yC
Cuts at: 0 1614 2154
Size: 1614 540
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 392 422 1095 1125 2154
Size: 392 30 673 30 1029
Fragments arranged by size:
1029 673 392 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1943 2154
Size: 1943 211
BseYI C'CCAG_C
Cuts at: 0 736 1241 1470 1492 2154
Size: 736 505 229 22 662
Fragments arranged by size:
736 662 505 229 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 2154
Size: 240 1914
BsiEI CG_ry'CG
Cuts at: 0 1281 2154
Size: 1281 873
BsiHKAI G_wGCw'C
Cuts at: 0 29 450 630 747 2154
Size: 29 421 180 117 1407
Fragments arranged by size:
1407 421 180 117 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1507 1605 2112 2154
Size: 1507 98 507 42
Fragments arranged by size:
1507 507 98 42
Bsp1286I G_dGCh'C
Cuts at: 0 29 450 630 747 1183 2154
Size: 29 421 180 117 436 971
Fragments arranged by size:
971 436 421 180 117 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 199 706 2154
Size: 199 507 1448
Fragments arranged by size:
1448 507 199
BsrBI CCG'CTC
Cuts at: 0 1235 2154
Size: 1235 919
BsrDI GCAATG_nn'
Cuts at: 0 1269 1919 2154
Size: 1269 650 235
Fragments arranged by size:
1269 650 235
BsrFI r'CCGG_y
Cuts at: 0 499 1301 1814 2154
Size: 499 802 513 340
Fragments arranged by size:
802 513 499 340
BsrGI T'GTAC_A
Cuts at: 0 1199 2154
Size: 1199 955
BssSI C'ACGA_G
Cuts at: 0 1170 2154
Size: 1170 984
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 584 758 2154
Size: 534 50 174 1396
Fragments arranged by size:
1396 534 174 50
BstEII G'GTnAC_C
Cuts at: 0 1986 2105 2154
Size: 1986 119 49
Fragments arranged by size:
1986 119 49
BstXI CCAn_nnnn'nTGG
Cuts at: 0 124 301 1258 1528 1950 2154
Size: 124 177 957 270 422 204
Fragments arranged by size:
957 422 270 204 177 124
BstYI r'GATC_y
Cuts at: 0 956 1023 1573 2154
Size: 956 67 550 581
Fragments arranged by size:
956 581 550 67
Bsu36I CC'TnA_GG
Cuts at: 0 74 2154
Size: 74 2080
BtgI C'CryG_G
Cuts at: 0 47 294 2154
Size: 47 247 1860
Fragments arranged by size:
1860 247 47
BtsI GCAGTG_nn'
Cuts at: 0 111 559 2154
Size: 111 448 1595
Fragments arranged by size:
1595 448 111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 183 218 2154
Size: 183 35 1936
Fragments arranged by size:
1936 183 35
DraI TTT'AAA
Cuts at: 0 573 2154
Size: 573 1581
EaeI y'GGCC_r
Cuts at: 0 1278 1303 1899 2154
Size: 1278 25 596 255
Fragments arranged by size:
1278 596 255 25
EagI C'GGCC_G
Cuts at: 0 1278 2154
Size: 1278 876
EarI CTCTTCn'nnn_
Cuts at: 0 504 1625 1676 2154
Size: 504 1121 51 478
Fragments arranged by size:
1121 504 478 51
EcoICRI GAG'CTC
Cuts at: 0 27 2154
Size: 27 2127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 730 888 2059 2078 2154
Size: 471 259 158 1171 19 76
Fragments arranged by size:
1171 471 259 158 76 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2017 2154
Size: 2017 137
EcoO109I rG'GnC_Cy
Cuts at: 0 78 594 1341 1555 2154
Size: 78 516 747 214 599
Fragments arranged by size:
747 599 516 214 78
EcoRV GAT'ATC
Cuts at: 0 516 1449 2154
Size: 516 933 705
Fragments arranged by size:
933 705 516
HaeII r_GCGC'y
Cuts at: 0 387 535 544 2046 2154
Size: 387 148 9 1502 108
Fragments arranged by size:
1502 387 148 108 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 336 368 392 424 1095 1127 1146 1178
Size: 336 32 24 32 671 32 19 32
Cuts at: 1178 1655 1687 2154
Size: 477 32 467
Fragments arranged by size:
671 477 467 336 32 32 32 32
32 24 19
HincII GTy'rAC
Cuts at: 0 439 2154
Size: 439 1715
HindIII A'AGCT_T
Cuts at: 0 1645 2154
Size: 1645 509
KpnI G_GTAC'C
Cuts at: 0 1137 2154
Size: 1137 1017
MfeI C'AATT_G
Cuts at: 0 357 492 2154
Size: 357 135 1662
Fragments arranged by size:
1662 357 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1351 1931 2154
Size: 1246 105 580 223
Fragments arranged by size:
1246 580 223 105
MscI TGG'CCA
Cuts at: 0 1901 2154
Size: 1901 253
MslI CAynn'nnrTG
Cuts at: 0 122 299 1355 2154
Size: 122 177 1056 799
Fragments arranged by size:
1056 799 177 122
MspA1I CmG'CkG
Cuts at: 0 273 740 1277 1865 2154
Size: 273 467 537 588 289
Fragments arranged by size:
588 537 467 289 273
NaeI GCC'GGC
Cuts at: 0 501 2154
Size: 501 1653
NcoI C'CATG_G
Cuts at: 0 47 294 2154
Size: 47 247 1860
Fragments arranged by size:
1860 247 47
NdeI CA'TA_TG
Cuts at: 0 1971 2154
Size: 1971 183
NgoMIV G'CCGG_C
Cuts at: 0 499 2154
Size: 499 1655
NspI r_CATG'y
Cuts at: 0 1414 1908 1950 2154
Size: 1414 494 42 204
Fragments arranged by size:
1414 494 204 42
PciI A'CATG_T
Cuts at: 0 1946 2154
Size: 1946 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 812 2154
Size: 812 1342
PfoI T'CCnGG_A
Cuts at: 0 707 959 2154
Size: 707 252 1195
Fragments arranged by size:
1195 707 252
PpuMI rG'GwC_Cy
Cuts at: 0 78 594 1555 2154
Size: 78 516 961 599
Fragments arranged by size:
961 599 516 78
PvuII CAG'CTG
Cuts at: 0 740 2154
Size: 740 1414
SacI G_AGCT'C
Cuts at: 0 29 2154
Size: 29 2125
SapI GCTCTTCn'nnn_
Cuts at: 0 1625 2154
Size: 1625 529
SexAI A'CCwGG_T
Cuts at: 0 921 2154
Size: 921 1233
SphI G_CATG'C
Cuts at: 0 1414 2154
Size: 1414 740
StuI AGG'CCT
Cuts at: 0 1658 2154
Size: 1658 496
StyI C'CwwG_G
Cuts at: 0 11 47 294 1086 1418 2154
Size: 11 36 247 792 332 736
Fragments arranged by size:
792 736 332 247 36 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 104 419 859 1154 1410 1940 2154
Size: 104 315 440 295 256 530 214
Fragments arranged by size:
530 440 315 295 256 214 104
TatI w'GTAC_w
Cuts at: 0 370 608 1199 2154
Size: 370 238 591 955
Fragments arranged by size:
955 591 370 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 2154
Size: 538 32 1584
Fragments arranged by size:
1584 538 32
Tth111I GACn'n_nGTC
Cuts at: 0 406 1107 2154
Size: 406 701 1047
Fragments arranged by size:
1047 701 406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 658 2154
Size: 658 1496
XmnI GAAnn'nnTTC
Cuts at: 0 149 2063 2154
Size: 149 1914 91
Fragments arranged by size:
1914 149 91
ZraI GAC'GTC
Cuts at: 0 1615 2154
Size: 1615 539
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AlwNI ApaLI
AseI BanI BanII BbsI BglII Bme1580I BmgBI BplI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII HindIII KpnI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PpuMI PvuII SacI
SapI SexAI SphI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI AleI AlfI AloI
ApaI ApoI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI
BmrI BmtI BpuEI BsaAI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BssHII BstBI BstZ17I BtgZI ClaI DraIII
DrdI EciI EcoRI FalI FseI FspI FspAI HpaI
KasI MluI NarI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI