(Circular) (Six-base) MAPSORT of: pFN21AA0044.seq Check: 1358 from: 1 to: 6130 pFN21AA0044 6130 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2760 3473 1937 Size: 823 713 4594 Fragments arranged by size: 4594 823 713 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 2351 4257 4630 1563 Size: 788 1906 373 3063 Fragments arranged by size: 3063 1906 788 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3176 4193 5577 5845 1632 Size: 1544 1017 1384 268 1917 Fragments arranged by size: 1917 1544 1384 1017 268 AflII C'TTAA_G Cuts at: 820 1017 2398 820 Size: 197 1381 4552 Fragments arranged by size: 4552 1381 197 AflIII A'CryG_T Cuts at: 1203 2280 5029 6006 1203 Size: 1077 2749 977 1327 Fragments arranged by size: 2749 1327 1077 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 2865 4859 2865 Size: 1994 4136 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2940 2972 2940 Size: 32 6098 AlwNI CAG_nnn'CTG Cuts at: 3873 5445 3873 Size: 1572 4558 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2324 2390 2549 2930 3439 3665 3817 5926 Size: 831 66 159 381 509 226 152 2109 Cuts at: 5926 1493 Size: 1697 Fragments arranged by size: 2109 1697 831 509 381 226 159 152 66 AseI AT'TA_AT Cuts at: 160 1032 2670 4684 160 Size: 872 1638 2014 1606 Fragments arranged by size: 2014 1638 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2502 3455 1870 Size: 73 559 953 4545 Fragments arranged by size: 4545 953 559 73 AvrII C'CTAG_G Cuts at: 2554 2554 Size: 6130 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3451 4906 618 Size: 325 217 627 1664 1455 1842 Fragments arranged by size: 1842 1664 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2484 3013 3449 1391 Size: 1093 529 436 4072 Fragments arranged by size: 4072 1093 529 436 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3055 3089 4321 4355 1903 Size: 34 1118 34 1232 34 3678 Fragments arranged by size: 3678 1232 1118 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 2083 2232 2452 1548 Size: 535 149 220 5226 Fragments arranged by size: 5226 535 220 149 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 4191 5347 1790 Size: 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3430 3430 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2965 4790 1239 Size: 1161 565 1825 2579 Fragments arranged by size: 2579 1825 1161 565 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 4255 5120 5418 2581 Size: 1674 865 298 3293 Fragments arranged by size: 3293 1674 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5905 Fragments arranged by size: 5905 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 2769 5333 5829 2769 Size: 2564 496 3070 Fragments arranged by size: 3070 2564 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2810 1163 Size: 573 1074 4483 Fragments arranged by size: 4483 1074 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3427 3580 4341 5369 1540 Size: 275 186 1426 153 761 1028 2301 Fragments arranged by size: 2301 1426 1028 761 275 186 153 BsiHKAI G_wGCw'C Cuts at: 2484 2703 3013 4191 4276 5347 2484 Size: 219 310 1178 85 1071 3267 Fragments arranged by size: 3267 1178 1071 310 219 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 2703 3013 3449 4191 4276 5347 Size: 399 694 219 310 436 742 85 1071 Cuts at: 5347 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 694 436 399 310 219 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 3039 4019 5749 3039 Size: 980 1730 3420 Fragments arranged by size: 3420 1730 980 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2321 4017 1394 Size: 429 498 1696 3507 Fragments arranged by size: 3507 1696 498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3197 96 Size: 3101 3029 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 2287 4934 2287 Size: 2647 3483 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6130 BstYI r'GATC_y Cuts at: 1972 2497 3310 3460 3827 4212 4229 5681 5767 Size: 525 813 150 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 813 525 385 367 346 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3357 3357 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 4597 Fragments arranged by size: 4597 939 552 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3391 3646 4440 4460 3391 Size: 255 794 20 5061 Fragments arranged by size: 5061 794 255 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2156 2743 2156 Size: 587 5543 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2852 2967 Size: 54 1425 50 275 18 189 833 115 Cuts at: 2967 3424 3577 4466 6095 8 Size: 457 153 889 1629 43 Fragments arranged by size: 1629 1425 889 833 457 275 189 153 115 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3424 3577 1537 Size: 275 1612 153 4090 Fragments arranged by size: 4090 1612 275 153 EarI CTCTTCn'nnn_ Cuts at: 1353 2691 2895 2960 3167 4060 1353 Size: 1338 204 65 207 893 3423 Fragments arranged by size: 3423 1338 893 207 204 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2482 3011 2482 Size: 529 5601 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2965 3176 4193 4790 5577 5845 Size: 393 768 565 211 1017 597 787 268 Cuts at: 5845 1239 Size: 1524 Fragments arranged by size: 1524 1017 787 768 597 565 393 268 211 EcoO109I rG'GnC_Cy Cuts at: 3361 3361 Size: 6130 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2111 2728 3397 2111 Size: 617 669 4844 Fragments arranged by size: 4844 669 617 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 5277 1678 Size: 3599 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2556 2588 2851 2883 4771 Size: 32 135 32 984 32 263 32 1888 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1888 984 263 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2278 3474 3720 669 Size: 988 281 340 1196 246 3079 Fragments arranged by size: 3079 1196 988 340 281 246 HindIII A'AGCT_T Cuts at: 748 2093 748 Size: 1345 4785 HpaI GTT'AAC Cuts at: 2278 3720 2278 Size: 1442 4688 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2449 5244 5428 1305 Size: 1144 2795 184 2007 Fragments arranged by size: 2795 2007 1144 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2854 2969 10 Size: 54 1425 343 1022 115 3171 Fragments arranged by size: 3171 1425 1022 343 115 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2628 3170 4088 4447 4606 518 Size: 648 843 619 542 918 359 159 2042 Fragments arranged by size: 2042 918 843 648 619 542 359 159 MspA1I CmG'CkG Cuts at: 1606 2773 3546 4223 5371 5616 1606 Size: 1167 773 677 1148 245 2120 Fragments arranged by size: 2120 1167 1148 773 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 2265 387 Size: 1878 4252 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2284 3488 5033 1132 Size: 97 1055 1204 1545 2229 Fragments arranged by size: 2229 1545 1204 1055 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2280 5029 2280 Size: 2749 3381 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 1108 Size: 939 5191 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3361 3361 Size: 6130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 3398 3482 830 Size: 2568 84 3478 Fragments arranged by size: 3478 2568 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2773 2773 Size: 6130 SacI G_AGCT'C Cuts at: 2484 3013 2484 Size: 529 5601 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 2691 3167 2691 Size: 476 5654 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2375 3394 3478 4613 5294 5485 826 Size: 219 1330 1019 84 1135 681 191 1471 Fragments arranged by size: 1471 1330 1135 1019 681 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2398 2560 4234 5135 5397 820 Size: 197 926 455 162 1674 901 262 1553 Fragments arranged by size: 1674 1553 926 901 455 262 197 162 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 2655 4054 5 Size: 47 2603 1399 2081 Fragments arranged by size: 2603 2081 1399 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 2554 3381 3569 513 Size: 552 939 550 827 188 3074 Fragments arranged by size: 3074 939 827 552 550 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2231 4157 4174 4327 4512 4957 325 Size: 1255 651 1926 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3197 4376 6088 Size: 274 80 33 51 1443 1220 1179 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 1712 1443 1220 1179 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6130 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3379 3379 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 3161 4259 3161 Size: 1098 5032 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI