Restriction map for
pFN21AB7869
##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BalI
BamHI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI HincII HindII HpaI KpnI
MaeI MamI MfeI MluI MluNI
MscI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 739 TCCGGA T^CCGGA
AlwI 306 GATCC GGATC(4/5)
AlwNI 304 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
AviII 549 TGCGCA TGC^GCA
BanI 84 GGCGCC G^G[CT][GA]CC
BbeI 84 GGCGCC GGCGC^C
BbrPI 979 CACGTG CAC^GTG
BcgI 373 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 648 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 243 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 243 GCTGAGC GC^T[ACGT]AGC
BsaAI 979 CACGTG [CT]AC^GT[GA]
BseAI 739 TCCGGA T^CCGGA
BsgI 193 CTGCAC GTGCAG(16/14)
BspEI 739 TCCGGA T^CCGGA
BsrBI 390 GAGCGG CCGCTC(-3/-3)
BsrFI 248 GCCGGC [GA]^CCGG[CT]
BstXI 1164 CCAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 305 AGATCC [GA]^GATC[CT]
CelII 243 GCTGAGC GC^T[ACGT]AGC
Cfr10I 248 GCCGGC [GA]^CCGG[CT]
DpnII 306 GATC ^GATC
DraII 48 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 964 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 407 TGGCCG [CT]^GGCC[GA]
EcoNI 345 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 48 GGGACCC [GA]G^G[ACGT]CC[CT]
FspI 549 TGCGCA TGC^GCA
HindIII 1179 AAGCTT A^AGCTT
KasI 84 GGCGCC G^GCGCC
KspI 161 CCGCGG CCGC^GG
MboI 306 GATC ^GATC
MflI 305 AGATCC [GA]^GATC[CT]
MroI 739 TCCGGA T^CCGGA
NaeI 248 GCCGGC GCC^GGC
NarI 84 GGCGCC GG^CGCC
NdeII 306 GATC ^GATC
NgoMIV 248 GCCGGC G^CCGGC
PmaCI 979 CACGTG CAC^GTG
PmlI 979 CACGTG CAC^GTG
PpuMI 48 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 205 CAGCTG CAG^CTG
SacII 161 CCGCGG CCGC^GG
SapI 1425 GAAGAGC GCTCTTC(1/4)
Sau3AI 306 GATC ^GATC
SfaNI 797 GCATC GCATC(5/9)
SfoI 84 GGCGCC GGC^GCC
XcmI 216 CCAGGAGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 305 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 37 GACGCC G[GA]^CG[CT]C
84 GGCGCC
AspHI 376 GAGCAC G[AT]GC[AT]^C
567 GAGCAC
AvaI 283 CCCGAG C^[CT]CG[GA]G
349 CCCGAG
BanII 148 GAGCCC G[GA]GC[CT]^C
419 GGGCTC
BbsI 127 GAAGAC GAAGAC(2/6)
835 GAAGAC
BcnI 136 CCCGG CC^[GC]GG
563 CCGGG
BpmI 310 CTGGAG CTGGAG(16/14)
325 CTGGAG
BpuAI 127 GAAGAC GAAGAC(2/6)
835 GAAGAC
BsaHI 37 GACGCC G[GA]^CG[CT]C
84 GGCGCC
BsaWI 231 ACCGGA [AT]^CCGG[AT]
739 TCCGGA
BsiHKAI 376 GAGCAC G[AT]GC[AT]^C
567 GAGCAC
BsmI 1336 GAATGC GAATGC(1/-1)
1417 GAATGC
BsoBI 283 CCCGAG C^[CT]CG[GA]G
349 CCCGAG
BsrI 1474 ACTGG ACTGG(1/-1)
1558 ACTGG
DraI 1324 TTTAAA TTT^AAA
1453 TTTAAA
DsaI 161 CCGCGG C^C[GA][CT]GG
410 CCGTGG
EcoT14I 74 CCAAGG C^C[AT][AT]GG
296 CCAAGG
HaeIII 408 GGCC GG^CC
917 GGCC
HgaI 37 GACGC GACGC(5/10)
79 GACGC
Hin1I 37 GACGCC G[GA]^CG[CT]C
84 GGCGCC
MaeII 973 ACGT A^CGT
980 ACGT
MslI 477 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1138 CACACAGGTG
MspA1I 161 CCGCGG C[AC]G^C[GT]G
205 CAGCTG
NciI 136 CCCGG CC^[GC]GG
563 CCGGG
PflMI 216 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
453 CCAGGGGATGG
PstI 424 CTGCAG CTGCA^G
537 CTGCAG
SfcI 424 CTGCAG C^T[GA][CT]AG
537 CTGCAG
StyI 74 CCAAGG C^C[AT][AT]GG
296 CCAAGG
Van91I 216 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
453 CCAGGGGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
Aor51HI ApoI Asp700I AvaII BbvI
BmyI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI EarI Eco47III EcoRI
EcoRII FauI Fnu4HI FokI HaeII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MspI MvaI MvnI
MwoI NlaIII NlaIV PleI RsaI
Sau96I ScrFI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI