(Circular) (Six-base) MAPSORT of: pFN21AB7869.seq Check: 6434 from: 1 to: 6388 pFN21AB7869 6388 bp With 182 enzymes: SgfI * October 20, 2009 10:56 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3731 1937 Size: 1794 4594 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2802 3172 3256 4451 5835 6103 1632 Size: 1170 370 84 1195 1384 268 1917 Fragments arranged by size: 1917 1384 1195 1170 370 268 84 AfeI AGC'GCT Cuts at: 2439 3269 3446 2439 Size: 830 177 5381 Fragments arranged by size: 5381 830 177 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6191 AflIII A'CryG_T Cuts at: 1203 5287 6264 1203 Size: 4084 977 1327 Fragments arranged by size: 4084 1327 977 AgeI A'CCGG_T Cuts at: 5219 5219 Size: 6388 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 2486 3147 1166 Size: 1320 661 4407 Fragments arranged by size: 4407 1320 661 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2886 1184 Size: 1702 4686 AlwNI CAG_nnn'CTG Cuts at: 2314 4131 5703 2314 Size: 1817 1572 2999 Fragments arranged by size: 2999 1817 1572 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 2008 2634 2773 3280 3555 3639 3697 3923 Size: 515 626 139 507 275 84 58 226 Cuts at: 3923 4075 6184 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 626 515 507 275 226 152 139 84 58 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2288 2354 3713 1870 Size: 73 345 66 1359 4545 Fragments arranged by size: 4545 1359 345 73 66 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 3709 5164 618 Size: 325 217 627 302 1620 1455 1842 Fragments arranged by size: 1842 1620 1455 627 325 302 217 BanII G_rGCy'C Cuts at: 1391 2157 2428 3707 1391 Size: 766 271 1279 4072 Fragments arranged by size: 4072 1279 766 271 BbeI G_GCGC'C Cuts at: 2093 2093 Size: 6388 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2139 2847 928 Size: 971 240 708 4469 Fragments arranged by size: 4469 971 708 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2367 2401 4579 4613 1903 Size: 34 430 34 2178 34 3678 Fragments arranged by size: 3678 2178 430 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4280 5490 4280 Size: 1210 5178 BclI T'GATC_A Cuts at: 1548 1548 Size: 6388 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2659 4999 136 Size: 107 122 71 631 605 987 2340 1525 Fragments arranged by size: 2340 1525 987 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 2249 3805 2249 Size: 1556 4832 Bme1580I G_kGCm'C Cuts at: 1790 4449 5605 1790 Size: 2659 1156 2573 Fragments arranged by size: 2659 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2979 1318 Size: 1661 4727 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 3573 4119 Size: 416 53 83 399 24 2072 84 546 Cuts at: 4119 5077 442 Size: 958 1753 Fragments arranged by size: 2072 1753 958 546 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6388 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2314 2152 Size: 162 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2351 5048 1239 Size: 1097 15 2697 2579 Fragments arranged by size: 2697 2579 1097 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4513 5378 5676 4513 Size: 865 298 5225 Fragments arranged by size: 5225 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2278 2742 3038 5051 882 Size: 617 779 464 296 2013 2219 Fragments arranged by size: 2219 2013 779 617 464 296 BsaAI yAC'GTr Cuts at: 493 1204 2986 4179 493 Size: 711 1782 1193 2702 Fragments arranged by size: 2702 1782 1193 711 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2090 4195 4577 Size: 53 83 186 936 510 47 2105 382 Cuts at: 4577 5212 275 Size: 635 1451 Fragments arranged by size: 2105 1451 936 635 510 382 186 83 53 47 BsaWI w'CCGG_w Cuts at: 2236 2744 4821 5219 5493 5640 6051 2236 Size: 508 2077 398 274 147 411 2573 Fragments arranged by size: 2573 2077 508 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6163 Fragments arranged by size: 6163 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2417 2519 1186 Size: 1186 45 102 5055 Fragments arranged by size: 5055 1186 102 45 BseYI C'CCAG_C Cuts at: 2524 3665 5591 6087 2524 Size: 1141 1926 496 2825 Fragments arranged by size: 2825 1926 1141 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 1163 Size: 573 447 5368 Fragments arranged by size: 5368 573 447 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3838 4599 5627 1540 Size: 275 186 1837 761 1028 2301 Fragments arranged by size: 2301 1837 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2385 2576 4449 4534 5605 2385 Size: 191 1873 85 1071 3168 Fragments arranged by size: 3168 1873 1071 191 85 BsmI GAATG_Cn' Cuts at: 3347 3428 3899 3992 3347 Size: 81 471 93 5743 Fragments arranged by size: 5743 471 93 81 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2157 2385 2428 2576 3707 4449 4534 Size: 399 367 228 43 148 1131 742 85 Cuts at: 4534 5605 1391 Size: 1071 2174 Fragments arranged by size: 2174 1131 1071 742 399 367 228 148 85 43 BspEI T'CCGG_A Cuts at: 2744 6051 2744 Size: 3307 3081 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3743 844 Size: 2899 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2397 4275 1394 Size: 429 574 1878 3507 Fragments arranged by size: 3507 1878 574 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2253 5032 5219 1700 Size: 110 443 2779 187 2869 Fragments arranged by size: 2869 2779 443 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6388 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6388 BstBI TT'CG_AA Cuts at: 5192 5192 Size: 6388 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6388 BstXI CCAn_nnnn'nTGG Cuts at: 3176 3176 Size: 6388 BstYI r'GATC_y Cuts at: 1972 2310 3718 4085 4470 4487 5939 6025 6037 Size: 338 1408 367 385 17 1452 86 12 Cuts at: 6037 6383 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1408 385 367 346 338 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2166 2415 513 Size: 552 1101 249 4486 Fragments arranged by size: 4486 1101 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5649 Fragments arranged by size: 5649 683 56 BtsI GCAGTG_nn' Cuts at: 2044 2383 3904 4698 4718 2044 Size: 339 1521 794 20 3714 Fragments arranged by size: 3714 1521 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3331 3460 3692 3331 Size: 129 232 6027 Fragments arranged by size: 6027 232 129 DraIII CAC_nnn'GTG Cuts at: 2974 2974 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 3835 4724 Size: 54 1425 50 275 18 582 1423 889 Cuts at: 4724 6353 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1423 889 582 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3835 1537 Size: 275 2023 4090 Fragments arranged by size: 4090 2023 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2235 3092 3425 4318 1353 Size: 882 857 333 893 3423 Fragments arranged by size: 3423 893 882 857 333 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2571 4975 5349 5495 387 Size: 1064 138 982 2404 374 146 1280 Fragments arranged by size: 2404 1280 1064 982 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2351 2802 3172 3256 4451 5048 Size: 393 704 15 451 370 84 1195 597 Cuts at: 5048 5835 6103 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1195 787 704 597 451 393 370 268 84 15 EcoNI CCTnn'n_nnAGG Cuts at: 2354 2354 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 2054 2054 Size: 6388 EcoRI G'AATT_C Cuts at: 2008 3280 3555 3639 3697 4075 2008 Size: 1272 275 84 58 378 4321 Fragments arranged by size: 4321 1272 378 275 84 58 FspI TGC'GCA Cuts at: 2556 4894 2556 Size: 2338 4050 HaeII r_GCGC'y Cuts at: 1678 2093 2249 2441 2518 2692 3271 3448 5535 Size: 415 156 192 77 174 579 177 2087 Cuts at: 5535 1678 Size: 2531 Fragments arranged by size: 2531 2087 579 415 192 177 174 156 77 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 3489 3521 5029 Size: 32 135 32 863 32 1022 32 1508 Cuts at: 5029 5061 5103 5135 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 1022 863 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3732 3978 669 Size: 988 281 1794 246 3079 Fragments arranged by size: 3079 1794 988 281 246 HindIII A'AGCT_T Cuts at: 748 3184 748 Size: 2436 3952 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KasI G'GCGC_C Cuts at: 2089 2089 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2907 3390 3652 5502 5686 1305 Size: 1602 483 262 1850 184 2007 Fragments arranged by size: 2007 1850 1602 483 262 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4566 Fragments arranged by size: 4566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3147 4346 4705 4864 518 Size: 648 1320 661 1199 359 159 2042 Fragments arranged by size: 2042 1320 1199 661 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2168 2212 3804 4481 5629 5874 1606 Size: 562 44 1592 677 1148 245 2120 Fragments arranged by size: 2120 1592 1148 677 562 245 44 NaeI GCC'GGC Cuts at: 2255 2255 Size: 6388 NarI GG'CG_CC Cuts at: 2090 2090 Size: 6388 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5836 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NgoMIV G'CCGG_C Cuts at: 2253 2253 Size: 6388 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6388 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3746 5291 1132 Size: 97 2517 1545 2229 Fragments arranged by size: 2517 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2058 2458 1348 Size: 710 400 5278 Fragments arranged by size: 5278 710 400 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 2227 2464 1108 Size: 1119 237 5032 Fragments arranged by size: 5032 1119 237 PfoI T'CCnGG_A Cuts at: 2313 2567 2313 Size: 254 6134 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 2986 2986 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4457 4489 5970 6002 4457 Size: 32 1481 32 4843 Fragments arranged by size: 4843 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2054 2054 Size: 6388 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3078 3958 3078 Size: 880 5508 PstI C_TGCA'G Cuts at: 830 2433 2546 3740 830 Size: 1603 113 1194 3478 Fragments arranged by size: 3478 1603 1194 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2212 2212 Size: 6388 SacII CC_GC'GG Cuts at: 2169 2169 Size: 6388 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SanDI GG'GwC_CC Cuts at: 2054 2054 Size: 6388 SapI GCTCTTCn'nnn_ Cuts at: 3425 3425 Size: 6388 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2429 2542 3736 4871 5552 5743 826 Size: 219 1384 113 1194 1135 681 191 1471 Fragments arranged by size: 1471 1384 1194 1135 681 219 191 113 SfoI GGC'GCC Cuts at: 2091 2091 Size: 6388 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6388 SmaI CCC'GGG Cuts at: 1872 3715 1872 Size: 1843 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4492 5393 5655 820 Size: 197 926 2549 901 262 1553 Fragments arranged by size: 2549 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4179 493 Size: 3686 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3746 1132 Size: 2614 3774 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 2079 2301 3827 513 Size: 552 1014 222 1526 3074 Fragments arranged by size: 3074 1526 1014 552 222 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2362 4415 4432 4585 4770 5215 Size: 1255 470 312 2053 17 153 185 445 Cuts at: 5215 325 Size: 1498 Fragments arranged by size: 2053 1498 1255 470 445 312 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3614 4634 6346 Size: 274 80 33 51 1443 1637 1020 1712 Cuts at: 6346 96 Size: 138 Fragments arranged by size: 1712 1637 1443 1020 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2046 2078 3609 3641 6348 6380 2046 Size: 32 1531 32 2707 32 2054 Fragments arranged by size: 2707 2054 1531 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6388 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2228 2228 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3713 1870 Size: 1843 4545 XmnI GAAnn'nnTTC Cuts at: 3101 3185 3434 3518 4517 3101 Size: 84 249 84 999 4972 Fragments arranged by size: 4972 999 249 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SrfI SwaI