Restriction map for pFN21AB7869
 (Linear) (Six-base) MAPSORT of: KIBB7869.seq  Check: 2585  from: 1  to: 1683



KIBB7869.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1167    1251    1683 
   Size:        797     370      84     432

  Fragments arranged by size: 

                 797     432     370      84
 
AfeI AGC'GCT

Cuts at:      0     434    1264    1441    1683 
   Size:        434     830     177     242

  Fragments arranged by size: 

                 830     434     242     177
 
AleI CACnn'nnGTG

Cuts at:      0     481    1142    1683 
   Size:        481     661     541

  Fragments arranged by size: 

                 661     541     481
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     881    1683 
   Size:        881     802
 
AlwNI CAG_nnn'CTG

Cuts at:      0     309    1683 
   Size:        309    1374
 
ApoI r'AATT_y

Cuts at:      0       3     629     768    1275    1550    1634    1683 
   Size:          3     626     139     507     275      84      49

  Fragments arranged by size: 

                 626     507     275     139      84      49       3
 
AvaI C'yCGr_G

Cuts at:      0     283     349    1683 
   Size:        283      66    1334

  Fragments arranged by size: 

                1334     283      66
 
BanI G'GyrC_C

Cuts at:      0      84    1683 
   Size:         84    1599
 
BanII G_rGCy'C

Cuts at:      0     152     423    1683 
   Size:        152     271    1260

  Fragments arranged by size: 

                1260     271     152
 
BbeI G_GCGC'C

Cuts at:      0      88    1683 
   Size:         88    1595
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     134     842    1683 
   Size:        134     708     841

  Fragments arranged by size: 

                 841     708     134
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     362     396    1683 
   Size:        362      34    1287

  Fragments arranged by size: 

                1287     362      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     654    1683 
   Size:        654    1029
 
BlpI GC'TnA_GC

Cuts at:      0     244    1683 
   Size:        244    1439
 
BmgBI CAC'GTC

Cuts at:      0     974    1683 
   Size:        974     709
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1484    1568    1683 
   Size:       1484      84     115

  Fragments arranged by size: 

                1484     115      84
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     147     309    1683 
   Size:        147     162    1374

  Fragments arranged by size: 

                1374     162     147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     346    1683 
   Size:        331      15    1337

  Fragments arranged by size: 

                1337     331      15
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     273     737    1033    1683 
   Size:        273     464     296     650

  Fragments arranged by size: 

                 650     464     296     273
 
BsaAI yAC'GTr

Cuts at:      0     981    1683 
   Size:        981     702
 
BsaHI Gr'CG_yC

Cuts at:      0      38      85    1683 
   Size:         38      47    1598

  Fragments arranged by size: 

                1598      47      38
 
BsaWI w'CCGG_w

Cuts at:      0     231     739    1683 
   Size:        231     508     944

  Fragments arranged by size: 

                 944     508     231
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     367     412     514    1683 
   Size:        367      45     102    1169

  Fragments arranged by size: 

                1169     367     102      45
 
BseYI C'CCAG_C

Cuts at:      0     519    1660    1683 
   Size:        519    1141      23

  Fragments arranged by size: 

                1141     519      23
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1683 
   Size:        178    1505
 
BsiHKAI G_wGCw'C

Cuts at:      0     380     571    1683 
   Size:        380     191    1112

  Fragments arranged by size: 

                1112     380     191
 
BsmI GAATG_Cn'

Cuts at:      0    1342    1423    1683 
   Size:       1342      81     260

  Fragments arranged by size: 

                1342     260      81
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     380     423     571    1683 
   Size:        152     228      43     148    1112

  Fragments arranged by size: 

                1112     228     152     148      43
 
BspEI T'CCGG_A

Cuts at:      0     739    1683 
   Size:        739     944
 
BsrBI CCG'CTC

Cuts at:      0     392    1683 
   Size:        392    1291
 
BsrFI r'CCGG_y

Cuts at:      0     248    1683 
   Size:        248    1435
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1171    1683 
   Size:       1171     512
 
BstYI r'GATC_y

Cuts at:      0     305    1683 
   Size:        305    1378
 
BtgI C'CryG_G

Cuts at:      0     161     410    1683 
   Size:        161     249    1273

  Fragments arranged by size: 

                1273     249     161
 
BtsI GCAGTG_nn'

Cuts at:      0      39     378    1683 
   Size:         39     339    1305

  Fragments arranged by size: 

                1305     339      39
 
DraI TTT'AAA

Cuts at:      0    1326    1455    1683 
   Size:       1326     129     228

  Fragments arranged by size: 

                1326     228     129
 
DraIII CAC_nnn'GTG

Cuts at:      0     969    1683 
   Size:        969     714
 
EaeI y'GGCC_r

Cuts at:      0     407    1683 
   Size:        407    1276
 
EarI CTCTTCn'nnn_

Cuts at:      0     230    1087    1420    1683 
   Size:        230     857     333     263

  Fragments arranged by size: 

                 857     333     263     230
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     566    1683 
   Size:        566    1117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     346     797    1167    1251    1683 
   Size:        331      15     451     370      84     432

  Fragments arranged by size: 

                 451     432     370     331      84      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     349    1683 
   Size:        349    1334
 
EcoO109I rG'GnC_Cy

Cuts at:      0      49    1683 
   Size:         49    1634
 
EcoRI G'AATT_C

Cuts at:      0       3    1275    1550    1634    1683 
   Size:          3    1272     275      84      49

  Fragments arranged by size: 

                1272     275      84      49       3
 
FspI TGC'GCA

Cuts at:      0     551    1683 
   Size:        551    1132
 
HaeII r_GCGC'y

Cuts at:      0      88     244     436     513     687    1266    1443    1683 
   Size:         88     156     192      77     174     579     177     240

  Fragments arranged by size: 

                 579     240     192     177     174     156      88      77
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     430     462    1484    1516    1683 
   Size:        430      32    1022      32     167

  Fragments arranged by size: 

                1022     430     167      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1179    1683 
   Size:       1179     504
 
KasI G'GCGC_C

Cuts at:      0      84    1683 
   Size:         84    1599
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     902    1385    1647    1683 
   Size:        902     483     262      36

  Fragments arranged by size: 

                 902     483     262      36
 
MslI CAynn'nnrTG

Cuts at:      0     481    1142    1683 
   Size:        481     661     541

  Fragments arranged by size: 

                 661     541     481
 
MspA1I CmG'CkG

Cuts at:      0     163     207    1683 
   Size:        163      44    1476

  Fragments arranged by size: 

                1476     163      44
 
NaeI GCC'GGC

Cuts at:      0     250    1683 
   Size:        250    1433
 
NarI GG'CG_CC

Cuts at:      0      85    1683 
   Size:         85    1598
 
NgoMIV G'CCGG_C

Cuts at:      0     248    1683 
   Size:        248    1435
 
PasI CC'CwG_GG

Cuts at:      0      53     453    1683 
   Size:         53     400    1230

  Fragments arranged by size: 

                1230     400      53
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     222     459    1683 
   Size:        222     237    1224

  Fragments arranged by size: 

                1224     237     222
 
PfoI T'CCnGG_A

Cuts at:      0     308     562    1683 
   Size:        308     254    1121

  Fragments arranged by size: 

                1121     308     254
 
PmlI CAC'GTG

Cuts at:      0     981    1683 
   Size:        981     702
 
PpuMI rG'GwC_Cy

Cuts at:      0      49    1683 
   Size:         49    1634
 
PsiI TTA'TAA

Cuts at:      0    1073    1683 
   Size:       1073     610
 
PstI C_TGCA'G

Cuts at:      0     428     541    1683 
   Size:        428     113    1142

  Fragments arranged by size: 

                1142     428     113
 
PvuII CAG'CTG

Cuts at:      0     207    1683 
   Size:        207    1476
 
SacII CC_GC'GG

Cuts at:      0     164    1683 
   Size:        164    1519
 
SanDI GG'GwC_CC

Cuts at:      0      49    1683 
   Size:         49    1634
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1420    1683 
   Size:       1420     263
 
SfcI C'TryA_G

Cuts at:      0     424     537    1683 
   Size:        424     113    1146

  Fragments arranged by size: 

                1146     424     113
 
SfoI GGC'GCC

Cuts at:      0      86    1683 
   Size:         86    1597
 
StyI C'CwwG_G

Cuts at:      0      74     296    1683 
   Size:         74     222    1387

  Fragments arranged by size: 

                1387     222      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      45     357    1683 
   Size:         45     312    1326

  Fragments arranged by size: 

                1326     312      45
 
TatI w'GTAC_w

Cuts at:      0    1609    1683 
   Size:       1609      74
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      41      73    1604    1636    1683 
   Size:         41      32    1531      32      47

  Fragments arranged by size: 

                1531      47      41      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     223    1683 
   Size:        223    1460
 
XmnI GAAnn'nnTTC

Cuts at:      0    1096    1180    1429    1513    1683 
   Size:       1096      84     249      84     170

  Fragments arranged by size: 

                1096     249     170      84      84
 

 Enzymes that do cut:

     AcuI     AfeI     AleI     AlfI    AlwNI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BcgI     BglI     BlpI    BmgBI     BmrI
     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BsrBI    BsrFI    BstXI
    BstYI     BtgI     BtsI     DraI   DraIII     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I  HindIII
     KasI     MmeI     MslI   MspA1I     NaeI     NarI   NgoMIV     PasI
    PflMI     PfoI     PmlI    PpuMI     PsiI     PstI    PvuII    SacII
    SanDI     SapI     SfcI     SfoI     StyI    TaqII     TatI     TstI
     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI    BglII Bme1580I
     BmtI   Bpu10I    BpuEI    BsaBI    BsaXI    BsiEI    BsiWI    BsmBI
    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI     EagI
  EcoICRI    EcoRV     FalI     FseI    FspAI   HincII     HpaI     KpnI
     MfeI     MluI     MscI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI     SalI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI
     XmaI     ZraI
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