Restriction map for
pFN21AB9574
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BglII
BlnI BsaAI BsaBI BsaI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 210 TCCGGA T^CCGGA
AcyI 33 GGCGTC G[GA]^CG[CT]C
AfaI 989 GTAC GT^AC
AflIII 1010 ACACGT A^C[GA][CT]GT
Alw44I 969 GTGCAC G^TGCAC
AlwI 615 GATCC GGATC(4/5)
AlwNI 220 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 940 GGGCCC GGGCC^C
ApaLI 969 GTGCAC G^TGCAC
AspHI 969 GTGCAC G[AT]GC[AT]^C
BalI 947 TGGCCA TGG^CCA
BcgI 601 CGACCTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1113 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1113 GCTCAGC GC^T[ACGT]AGC
BsaHI 33 GGCGTC G[GA]^CG[CT]C
BseAI 210 TCCGGA T^CCGGA
BsiEI 65 CGGCCG CG[GA][CT]^CG
BsiHKAI 969 GTGCAC G[AT]GC[AT]^C
BspEI 210 TCCGGA T^CCGGA
BsrBI 858 CCGCTC CCGCTC(-3/-3)
BsrDI 1091 GCAATG GCAATG(2/0)
BstEII 981 GGTCACC G^GT[ACGT]ACC
BstPI 981 GGTCACC G^GT[ACGT]ACC
BstYI 614 AGATCC [GA]^GATC[CT]
CelII 1113 GCTCAGC GC^T[ACGT]AGC
DrdI 154 GACCTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 65 CGGCCG C^GGCCG
EclXI 65 CGGCCG C^GGCCG
Eco52I 65 CGGCCG C^GGCCG
EcoO65I 981 GGTCACC G^GT[ACGT]ACC
EcoT14I 944 CCATGG C^C[AT][AT]GG
Hin1I 33 GGCGTC G[GA]^CG[CT]C
HincII 130 GTCAAC GT[CT]^[GA]AC
HindII 130 GTCAAC GT[CT]^[GA]AC
MaeII 1012 ACGT A^CGT
MflI 614 AGATCC [GA]^GATC[CT]
MluNI 947 TGGCCA TGG^CCA
MroI 210 TCCGGA T^CCGGA
MscI 947 TGGCCA TGG^CCA
MslI 972 CACTCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 944 CCATGG C^CATGG
PpuMI 886 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 989 GTAC GT^AC
StuI 710 AGGCCT AGG^CCT
StyI 944 CCATGG C^C[AT][AT]GG
TaqI 202 TCGA T^CGA
TfiI 422 GAATC G^A[AT]TC
TthHB8I 202 TCGA T^CGA
XcmI 746 CCAAGCCCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 614 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 57 CGCG CG^CG
135 CGCG
AvaI 9 CTCGGG C^[CT]CG[GA]G
1036 CCCGAG
BfaI 514 CTAG C^TAG
577 CTAG
BsmBI 162 CGTCTC CGTCTC(1/5)
243 CGTCTC
BsmFI 60 GTCCC GGGAC(10/14)
292 GTCCC
BsoBI 9 CTCGGG C^[CT]CG[GA]G
1036 CCCGAG
BstUI 57 CGCG CG^CG
135 CGCG
BstXI 950 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1124 CCACGCTGCTGG
DpnII 615 GATC ^GATC
664 GATC
DsaI 185 CCACGG C^C[GA][CT]GG
944 CCATGG
EarI 409 GAAGAG CTCTTC(1/4)
640 GAAGAG
EcoNI 555 CCTGCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
678 CCTGCCAGAGG
HgaI 34 GCGTC GACGC(5/10)
58 GCGTC
Ksp632I 409 GAAGAG CTCTTC(1/4)
640 GAAGAG
MaeI 514 CTAG C^TAG
577 CTAG
MaeIII 190 GTCAC ^GT[ACGT]AC
982 GTCAC
MboI 615 GATC ^GATC
664 GATC
MvnI 57 CGCG CG^CG
135 CGCG
NdeII 615 GATC ^GATC
664 GATC
Sau3AI 615 GATC ^GATC
664 GATC
SfaNI 773 GCATC GCATC(5/9)
826 GCATC
Tsp45I 190 GTCAC ^GT[GC]AC
982 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AvaII BanII BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV PleI PstI PvuII Sau96I
ScrFI SfcI TseI TspRI