Restriction map for
pFN21AB9519
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstYI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MflI MluI MroI MseI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Van91I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 607 GTCTAC GT^[AC][GT]AC
AfaI 79 GTAC GT^AC
Alw44I 275 GTGCAC G^TGCAC
AlwI 734 GGATC GGATC(4/5)
ApaI 754 GGGCCC GGGCC^C
ApaLI 275 GTGCAC G^TGCAC
AvaI 703 CCCGGG C^[CT]CG[GA]G
BanII 754 GGGCCC G[GA]GC[CT]^C
BcgI 869 CGATCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 779 GTATCC GTATCC(6/5)
BglI 298 GCCTTTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 13 ACCGGA [AT]^CCGG[AT]
BsoBI 703 CCCGGG C^[CT]CG[GA]G
BsrBI 33 GAGCGG CCGCTC(-3/-3)
BstAPI 660 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 896 GGTCACC G^GT[ACGT]ACC
BstF5I 980 GGATG GGATG(2/0)
BstPI 896 GGTCACC G^GT[ACGT]ACC
DraII 1034 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoNI 156 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1034 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 896 GGTCACC G^GT[ACGT]ACC
FokI 980 GGATG GGATG(9/13)
HaeII 37 GGCGCT [GA]GCGC^[CT]
HgaI 353 GCGTC GACGC(5/10)
HincII 1003 GTCAAC GT[CT]^[GA]AC
HindII 1003 GTCAAC GT[CT]^[GA]AC
HinfI 1056 GACTC G^A[ACGT]TC
MaeII 482 ACGT A^CGT
MfeI 1010 CAATTG C^AATTG
MunI 1010 CAATTG C^AATTG
PleI 1056 GACTC GAGTC(4/5)
PpuMI 1034 AGGACCT [GA]G^G[AT]CC[CT]
RsaI 79 GTAC GT^AC
SapI 257 GCTCTTC GCTCTTC(1/4)
SfiI 297 GGCCTTTATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 703 CCCGGG CCC^GGG
SphI 795 GCATGC GCATG^C
TaqI 629 TCGA T^CGA
Tsp509I 1011 AATT ^AATT
TthHB8I 629 TCGA T^CGA
XcmI 548 CCAATGACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 703 CCCGGG C^CCGGG
XmaI 703 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 420 CGCG CG^CG
867 CGCG
AocI 22 CCTGAGG CC^T[ACGT]AGG
1050 CCTGAGG
AspI 444 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
826 GACGAGGTC
BsgI 157 CTGCAC GTGCAG(16/14)
658 GTGCAG
BstUI 420 CGCG CG^CG
867 CGCG
Bsu36I 22 CCTGAGG CC^T[ACGT]AGG
1050 CCTGAGG
DpnII 735 GATC ^GATC
870 GATC
EarI 258 CTCTTC CTCTTC(1/4)
625 CTCTTC
Eco81I 22 CCTGAGG CC^T[ACGT]AGG
1050 CCTGAGG
EcoT14I 458 CCATGG C^C[AT][AT]GG
696 CCATGG
FauI 359 CCCGC CCCGC(4/6)
112 GCGGG
Ksp632I 258 CTCTTC CTCTTC(1/4)
625 CTCTTC
MboI 735 GATC ^GATC
870 GATC
MboII 259 TCTTC GAAGA(8/7)
626 TCTTC
MslI 429 CATCTGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
567 CATGCTTATG
MspA1I 282 CAGCTG C[AC]G^C[GT]G
847 CAGCTG
MvnI 420 CGCG CG^CG
867 CGCG
NaeI 739 GCCGGC GCC^GGC
806 GCCGGC
NcoI 458 CCATGG C^CATGG
696 CCATGG
NdeII 735 GATC ^GATC
870 GATC
NgoMIV 739 GCCGGC G^CCGGC
806 GCCGGC
PflFI 444 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
826 GACGAGGTC
PvuII 282 CAGCTG CAG^CTG
847 CAGCTG
Sau3AI 735 GATC ^GATC
870 GATC
SmlI 142 CTCAAG C^T[CT][GA]AG
1039 CTCAAG
StyI 458 CCATGG C^C[AT][AT]GG
696 CCATGG
Tth111I 444 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
826 GACGAGGTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanI BbvI BcnI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrFI BsrI BssKI BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DsaI EaeI EcoRII Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MaeIII MluNI MnlI MscI
MspI MvaI MwoI NciI NlaIII
NlaIV NspI Sau96I ScrFI SfaNI
TseI Tsp45I TspRI