(Circular) (Six-base) MAPSORT of: pFN21AB9519.seq Check: 3097 from: 1 to: 5824 pFN21AB9519 5824 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3634 278 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2613 3167 1937 Size: 676 554 4594 Fragments arranged by size: 4594 676 554 Acc65I G'GTAC_C Cuts at: 3145 3145 Size: 5824 AclI AA'CG_TT Cuts at: 1563 3951 4324 1563 Size: 2388 373 3063 Fragments arranged by size: 3063 2388 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2260 3887 5271 5539 1632 Size: 628 1627 1384 268 1917 Fragments arranged by size: 1917 1627 1384 628 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5627 AflIII A'CryG_T Cuts at: 1203 4723 5700 1203 Size: 3520 977 1327 Fragments arranged by size: 3520 1327 977 AgeI A'CCGG_T Cuts at: 4655 4655 Size: 5824 AhdI GACnn_n'nnGTC Cuts at: 4553 4553 Size: 5824 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5824 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2305 1184 Size: 1121 4703 AlwNI CAG_nnn'CTG Cuts at: 3567 5139 3567 Size: 1572 4252 ApaI G_GGCC'C Cuts at: 2763 2763 Size: 5824 ApaLI G'TGCA_C Cuts at: 2280 3881 5037 2280 Size: 1601 1156 3067 Fragments arranged by size: 3067 1601 1156 ApoI r'AATT_y Cuts at: 1493 3133 3359 3511 5620 1493 Size: 1640 226 152 2109 1697 Fragments arranged by size: 2109 1697 1640 226 152 AseI AT'TA_AT Cuts at: 160 1032 4378 160 Size: 872 3346 1606 Fragments arranged by size: 3346 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 AvaI C'yCGr_G Cuts at: 1870 1943 2708 3149 1870 Size: 73 765 441 4545 Fragments arranged by size: 4545 765 441 73 BamHI G'GATC_C Cuts at: 3154 3521 3154 Size: 367 5457 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2492 2671 3089 3145 4600 Size: 325 217 627 705 179 418 56 1455 Cuts at: 4600 618 Size: 1842 Fragments arranged by size: 1842 1455 705 627 418 325 217 179 56 BanII G_rGCy'C Cuts at: 1391 2763 3143 1391 Size: 1372 380 4072 Fragments arranged by size: 4072 1372 380 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4853 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2863 2897 4015 4049 1903 Size: 34 926 34 1118 34 3678 Fragments arranged by size: 3678 1118 926 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2795 3716 4926 2795 Size: 921 1210 3693 Fragments arranged by size: 3693 1210 921 BclI T'GATC_A Cuts at: 1548 1548 Size: 5824 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2309 4435 136 Size: 107 122 71 631 605 637 2126 1525 Fragments arranged by size: 2126 1525 637 631 605 122 107 71 BglII A'GATC_T Cuts at: 5819 5819 Size: 5824 BlpI GC'TnA_GC Cuts at: 3241 3241 Size: 5824 Bme1580I G_kGCm'C Cuts at: 1790 2161 2284 2495 2763 2958 3083 3094 3885 Size: 371 123 211 268 195 125 11 791 Cuts at: 3885 5041 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 791 371 268 211 195 125 123 11 BmgBI CAC'GTC Cuts at: 1318 2488 1318 Size: 1170 4654 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2831 3555 4513 Size: 416 53 83 399 24 1414 724 958 Cuts at: 4513 442 Size: 1753 Fragments arranged by size: 1753 1414 958 724 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5824 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2061 2061 Size: 5824 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4484 1239 Size: 3245 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 3029 3949 4814 5112 2132 Size: 897 920 865 298 2844 Fragments arranged by size: 2844 920 897 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4487 882 Size: 617 2988 2219 Fragments arranged by size: 2988 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3615 493 Size: 711 2411 2702 Fragments arranged by size: 2702 2411 711 BsaBI GATnn'nnATC Cuts at: 5818 5818 Size: 5824 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3631 4013 4648 275 Size: 53 83 186 936 2098 382 635 1451 Fragments arranged by size: 2098 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2018 4257 4655 4929 5076 5487 2018 Size: 2239 398 274 147 411 2355 Fragments arranged by size: 2355 2239 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 1178 Size: 30 165 30 1104 30 4465 Fragments arranged by size: 4465 1104 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2528 2618 2885 1186 Size: 859 483 90 267 4125 Fragments arranged by size: 4125 859 483 267 90 BseYI C'CCAG_C Cuts at: 2089 2563 2779 2854 3075 5027 5523 2089 Size: 474 216 75 221 1952 496 2390 Fragments arranged by size: 2390 1952 496 474 221 216 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2684 1163 Size: 573 411 537 4303 Fragments arranged by size: 4303 573 537 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3274 4035 5063 1540 Size: 275 186 1273 761 1028 2301 Fragments arranged by size: 2301 1273 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2148 2284 2319 2592 3885 3970 5041 2148 Size: 136 35 273 1293 85 1071 2931 Fragments arranged by size: 2931 1293 1071 273 136 85 35 BsmI GAATG_Cn' Cuts at: 3335 3428 3335 Size: 93 5731 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2148 2161 2284 2319 2495 2592 2763 Size: 399 358 13 123 35 176 97 171 Cuts at: 2763 2958 3083 3094 3143 3885 3970 5041 1391 Size: 195 125 11 49 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 358 195 176 171 125 123 97 85 49 35 13 11 BspEI T'CCGG_A Cuts at: 5487 5487 Size: 5824 BspHI T'CATG_A Cuts at: 3713 5443 3713 Size: 1730 4094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3179 844 Size: 2335 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2040 3711 1394 Size: 429 217 1671 3507 Fragments arranged by size: 3507 1671 429 217 BsrDI GCAATG_nn' Cuts at: 65 1248 4319 4493 65 Size: 1183 3071 174 1396 Fragments arranged by size: 3071 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2717 2744 2811 4468 4655 1700 Size: 110 907 27 67 1657 187 2869 Fragments arranged by size: 2869 1657 907 187 110 67 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5824 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5824 BssSI C'ACGA_G Cuts at: 3884 4896 3884 Size: 1012 4812 BstAPI GCAn_nnn'nTGC Cuts at: 794 2671 794 Size: 1877 3947 BstBI TT'CG_AA Cuts at: 4628 4628 Size: 5824 BstEII G'GTnAC_C Cuts at: 1184 2901 1184 Size: 1717 4107 BstYI r'GATC_y Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819 Size: 1182 367 385 17 1452 86 12 346 Cuts at: 5819 1972 Size: 1977 Fragments arranged by size: 1977 1452 1182 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2028 3056 2028 Size: 1028 4796 BtgI C'CryG_G Cuts at: 513 1065 2004 2257 2463 2701 2755 513 Size: 552 939 253 206 238 54 3582 Fragments arranged by size: 3582 939 552 253 238 206 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2362 2522 487 Size: 683 56 1136 160 3789 Fragments arranged by size: 3789 1136 683 160 56 BtsI GCAGTG_nn' Cuts at: 2879 2973 3340 4134 4154 2879 Size: 94 367 794 20 4549 Fragments arranged by size: 4549 794 367 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5824 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5789 DraI TTT'AAA Cuts at: 3128 3128 Size: 5824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4831 809 Size: 507 3515 1802 Fragments arranged by size: 3515 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2202 2310 Size: 54 1425 50 275 18 177 195 108 Cuts at: 2310 2373 2460 2719 2752 2970 3271 4160 5789 Size: 63 87 259 33 218 301 889 1629 Cuts at: 5789 8 Size: 43 Fragments arranged by size: 1629 1425 889 301 275 259 218 195 177 108 87 63 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3271 1537 Size: 275 1459 4090 Fragments arranged by size: 4090 1459 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2269 2636 3754 1353 Size: 916 367 1118 3423 Fragments arranged by size: 3423 1118 916 367 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4411 4785 4931 387 Size: 1064 138 2822 374 146 1280 Fragments arranged by size: 2822 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2260 3887 4484 5271 5539 1239 Size: 393 628 1627 597 787 268 1524 Fragments arranged by size: 1627 1524 787 628 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2165 2165 Size: 5824 EcoO109I rG'GnC_Cy Cuts at: 3040 3040 Size: 5824 EcoRI G'AATT_C Cuts at: 3133 3511 3133 Size: 378 5446 FspI TGC'GCA Cuts at: 4330 4330 Size: 5824 HaeII r_GCGC'y Cuts at: 1678 2046 4971 1678 Size: 368 2925 2531 Fragments arranged by size: 2925 2531 368 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373 Size: 32 135 32 2893 32 42 32 2626 Fragments arranged by size: 2893 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3010 3168 3414 669 Size: 988 281 1072 158 246 3079 Fragments arranged by size: 3079 1072 988 281 246 158 HindIII A'AGCT_T Cuts at: 748 748 Size: 5824 HpaI GTT'AAC Cuts at: 3414 3414 Size: 5824 KpnI G_GTAC'C Cuts at: 3149 3149 Size: 5824 MfeI C'AATT_G Cuts at: 3015 3423 3015 Size: 408 5416 MluI A'CGCG_T Cuts at: 5700 5700 Size: 5824 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4938 5122 1305 Size: 3633 184 2007 Fragments arranged by size: 3633 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2375 2462 2972 10 Size: 54 1425 343 177 366 87 510 2862 Fragments arranged by size: 2862 1425 510 366 343 177 87 54 MslI CAynn'nnrTG Cuts at: 518 1166 2438 2576 3782 4141 4300 518 Size: 648 1272 138 1206 359 159 2042 Fragments arranged by size: 2042 1272 1206 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2289 2854 3240 3917 5065 5310 1606 Size: 683 565 386 677 1148 245 2120 Fragments arranged by size: 2120 1148 683 677 565 386 245 NaeI GCC'GGC Cuts at: 2746 2813 2746 Size: 67 5757 NcoI C'CATG_G Cuts at: 513 1065 2004 2463 2701 513 Size: 552 939 459 238 3636 Fragments arranged by size: 3636 939 552 459 238 NdeI CA'TA_TG Cuts at: 387 387 Size: 5824 NgoMIV G'CCGG_C Cuts at: 2744 2811 2744 Size: 67 5757 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5824 NotI GC'GGCC_GC Cuts at: 3271 3271 Size: 5824 NspI r_CATG'y Cuts at: 1132 1229 2424 2804 2866 3182 4727 1132 Size: 97 1195 380 62 316 1545 2229 Fragments arranged by size: 2229 1545 1195 380 316 97 62 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5824 PciI A'CATG_T Cuts at: 4723 4723 Size: 5824 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5824 PmeI GTTT'AAAC Cuts at: 3128 3128 Size: 5824 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3893 3925 5406 5438 3893 Size: 32 1481 32 4279 Fragments arranged by size: 4279 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3040 3040 Size: 5824 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5824 PsiI TTA'TAA Cuts at: 3394 3394 Size: 5824 PspOMI G'GGCC_C Cuts at: 2759 2759 Size: 5824 PstI C_TGCA'G Cuts at: 830 3176 830 Size: 2346 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5824 PvuII CAG'CTG Cuts at: 2289 2854 2289 Size: 565 5259 SalI G'TCGA_C Cuts at: 1936 3166 1936 Size: 1230 4594 SapI GCTCTTCn'nnn_ Cuts at: 2269 2269 Size: 5824 SbfI CC_TGCA'GG Cuts at: 3176 3176 Size: 5824 ScaI AGT'ACT Cuts at: 4072 5784 4072 Size: 1712 4112 SfcI C'TryA_G Cuts at: 826 1045 3172 4307 4988 5179 826 Size: 219 2127 1135 681 191 1471 Fragments arranged by size: 2127 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2309 2309 Size: 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5824 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5824 SmaI CCC'GGG Cuts at: 1872 2710 3151 1872 Size: 838 441 4545 Fragments arranged by size: 4545 838 441 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 3044 3928 4829 5091 820 Size: 197 926 204 897 884 901 262 1553 Fragments arranged by size: 1553 926 901 897 884 262 204 197 SnaBI TAC'GTA Cuts at: 493 3615 493 Size: 3122 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5824 SphI G_CATG'C Cuts at: 1132 2804 3182 1132 Size: 1672 378 3774 Fragments arranged by size: 3774 1672 378 SspI AAT'ATT Cuts at: 5 52 3748 5 Size: 47 3696 2081 Fragments arranged by size: 3696 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5824 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 2701 3263 513 Size: 552 939 459 238 562 3074 Fragments arranged by size: 3074 939 562 552 459 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3851 3868 4021 4206 4651 325 Size: 1255 2271 17 153 185 445 1498 Fragments arranged by size: 2271 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4070 5782 96 Size: 274 80 33 51 1443 2093 1712 138 Fragments arranged by size: 2093 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2263 2295 2507 2539 5784 5816 2263 Size: 32 212 32 3245 32 2271 Fragments arranged by size: 3245 2271 212 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2452 2834 1316 Size: 1136 382 4306 Fragments arranged by size: 4306 1136 382 XbaI T'CTAG_A Cuts at: 3160 3160 Size: 5824 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5824 XmaI C'CCGG_G Cuts at: 1870 2708 3149 1870 Size: 838 441 4545 Fragments arranged by size: 4545 838 441 XmnI GAAnn'nnTTC Cuts at: 3953 3953 Size: 5824 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3632 276 Size: 53 83 186 936 2098 2468 Fragments arranged by size: 2468 2098 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfoI SrfI SwaI