Restriction map for
pFN21AB9518
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I MaeII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AlwI 15 GATCC GGATC(4/5)
Asp700I 514 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 634 CCCGGG C^[CT]CG[GA]G
BfaI 1070 CTAG C^TAG
BglI 21 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 993 AGATCT A^GATCT
BpmI 806 CTGGAG CTGGAG(16/14)
BsaI 310 GAGACC GGTCTC(1/5)
BseRI 1430 GAGGAG GAGGAG(10/8)
BsmFI 38 GTCCC GGGAC(10/14)
BsmI 1546 GAATGC GAATGC(1/-1)
BsoBI 634 CCCGGG C^[CT]CG[GA]G
BspHI 1577 TCATGA T^CATGA
BsrBI 331 GAGCGG CCGCTC(-3/-3)
BsrFI 54 GCCGGT [GA]^CCGG[CT]
BstAPI 451 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 54 GCCGGT [GA]^CCGG[CT]
EaeI 48 CGGCCA [CT]^GGCC[GA]
EcoRI 1392 GAATTC G^AATTC
EcoT22I 1003 ATGCAT ATGCA^T
FauI 18 CCCGC CCCGC(4/6)
HaeII 685 GGCGCT [GA]GCGC^[CT]
HgaI 1226 GCGTC GACGC(5/10)
KspI 22 CCGCGG CCGC^GG
MaeI 1070 CTAG C^TAG
NdeI 408 CATATG CA^TATG
NsiI 1003 ATGCAT ATGCA^T
NspI 143 GCATGC [GA]CATG^[CT]
PvuII 1566 CAGCTG CAG^CTG
RcaI 1577 TCATGA T^CATGA
SacI 1747 GAGCTC GAGCT^C
SacII 22 CCGCGG CCGC^GG
SexAI 1347 ACCTGGT A^CC[AT]GGT
SmaI 634 CCCGGG CCC^GGG
SphI 143 GCATGC GCATG^C
SspI 838 AATATT AAT^ATT
XcmI 1302 CCATGAAATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 634 CCCGGG C^CCGGG
XmaI 634 CCCGGG C^CCGGG
XmnI 514 GAACTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 23 CGCG CG^CG
46 CGCG
AspHI 809 GAGCAC G[AT]GC[AT]^C
1747 GAGCTC
BanII 1195 GGGCTC G[GA]GC[CT]^C
1747 GAGCTC
BbvI 451 GCAGC GCAGC(8/12)
1699 GCAGC
BsiHKAI 809 GAGCAC G[AT]GC[AT]^C
1747 GAGCTC
BstUI 23 CGCG CG^CG
46 CGCG
BstYI 14 AGATCC [GA]^GATC[CT]
993 AGATCT
Cac8I 50 GCCAGC GC[ACGT]^[ACGT]GC
143 GCATGC
CfoI 45 GCGC GCG^C
686 GCGC
DpnII 15 GATC ^GATC
994 GATC
HaeIII 49 GGCC GG^CC
1459 GGCC
HhaI 45 GCGC GCG^C
686 GCGC
HinP1I 45 GCGC G^CGC
686 GCGC
MboI 15 GATC ^GATC
994 GATC
MflI 14 AGATCC [GA]^GATC[CT]
993 AGATCT
MslI 1620 CACTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1626 CATGGCTGTG
MspA1I 22 CCGCGG C[AC]G^C[GT]G
1566 CAGCTG
MvnI 23 CGCG CG^CG
46 CGCG
NcoI 824 CCATGG C^CATGG
1625 CCATGG
NdeII 15 GATC ^GATC
994 GATC
PflMI 51 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1302 CCATGAAATGG
PstI 1141 CTGCAG CTGCA^G
1697 CTGCAG
Sau3AI 15 GATC ^GATC
994 GATC
SfaNI 1668 GATGC GCATC(5/9)
1643 GCATC
SfcI 1141 CTGCAG C^T[GA][CT]AG
1697 CTGCAG
SmlI 725 CTCAAG C^T[CT][GA]AG
1706 CTCAAG
TseI 451 GCAGC G^C[AT]GC
1699 GCAGC
Van91I 51 CCAGCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1302 CCATGAAATGG
XhoII 14 AGATCC [GA]^GATC[CT]
993 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbsI BcnI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DsaI EcoRII EcoT14I Fnu4HI
FokI HapII HinfI HpaII HphI
ItaI MaeIII MboII MnlI MseI
MspI MvaI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I