Restriction map for pFN21AB9518
 (Circular) (Six-base) MAPSORT of: pFN21AB9518.seq  Check: 7470  from: 1  to: 6496



pFN21AB9518  6496 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4306     278 
   Size:         53      83     186     936    2770    2468

  Fragments arranged by size: 

                2770    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3839    1937 
   Size:       1902    4594
 
Acc65I G'GTAC_C

Cuts at:   3817    3817 
   Size:       6496
 
AclI AA'CG_TT

Cuts at:   1563    4623    4996    1563 
   Size:       3060     373    3063

  Fragments arranged by size: 

                3063    3060     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2105    2145    2255    2882    4559    5943    6211    1632 
   Size:        473      40     110     627    1677    1384     268    1917

  Fragments arranged by size: 

                1917    1677    1384     627     473     268     110      40
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6299
 
AflIII A'CryG_T

Cuts at:   1203    5395    6372    1203 
   Size:       4192     977    1327

  Fragments arranged by size: 

                4192    1327     977
 
AgeI A'CCGG_T

Cuts at:   5327    5327 
   Size:       6496
 
AhdI GACnn_n'nnGTC

Cuts at:   5225    5225 
   Size:       6496
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6496
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6496
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3054    3086    3054 
   Size:         32    6464
 
AlwNI CAG_nnn'CTG

Cuts at:   4239    5811    4239 
   Size:       1572    4924
 
ApaLI G'TGCA_C

Cuts at:   4553    5709    4553 
   Size:       1156    5340
 
ApoI r'AATT_y

Cuts at:   1493    2981    3101    3286    3397    3557    3805    4031    4183 
   Size:       1488     120     185     111     160     248     226     152

Cuts at:   4183    6292    1493 
   Size:       2109    1697

  Fragments arranged by size: 

                2109    1697    1488     248     226     185     160     152
                 120     111
 
AseI AT'TA_AT

Cuts at:    160    1032    5050     160 
   Size:        872    4018    1606

  Fragments arranged by size: 

                4018    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6496
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2639    3821    1870 
   Size:         73     696    1182    4545

  Fragments arranged by size: 

                4545    1182     696      73
 
BamHI G'GATC_C

Cuts at:   3826    4193    3826 
   Size:        367    6129
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3817    5272     618 
   Size:        325     217     627    2030    1455    1842

  Fragments arranged by size: 

                2030    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3204    3756    3815    1391 
   Size:       1813     552      59    4072

  Fragments arranged by size: 

                4072    1813     552      59
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2093    2604    3200    3571     928 
   Size:        971     194     511     596     371    3853

  Fragments arranged by size: 

                3853     971     596     511     371     194
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4687    4721    1903 
   Size:         34    2750      34    3678

  Fragments arranged by size: 

                3678    2750      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4388    5598    4388 
   Size:       1210    5286
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6496
 
BfrBI ATG'CAT

Cuts at:   3010    3010 
   Size:       6496
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2032    5107     136 
   Size:        107     122      71     631     605     360    3075    1525

  Fragments arranged by size: 

                3075    1525     631     605     360     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2998    6491    2998 
   Size:       3493    3003
 
BlpI GC'TnA_GC

Cuts at:   3913    3913 
   Size:       6496
 
Bme1580I G_kGCm'C

Cuts at:   1790    2297    4557    5713    1790 
   Size:        507    2260    1156    2573

  Fragments arranged by size: 

                2573    2260    1156     507
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       6496
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2696    3207    3384 
   Size:        416      53      83     399      24    1279     511     177

Cuts at:   3384    4227    5185     442 
   Size:        843     958    1753

  Fragments arranged by size: 

                1753    1279     958     843     511     416     399     177
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6496
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2832    5156    1239 
   Size:       1593    2324    2579

  Fragments arranged by size: 

                2579    2324    1593
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2715    3696    4621    5486    5784    2715 
   Size:        981     925     865     298    3427

  Fragments arranged by size: 

                3427     981     925     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2309    5159     882 
   Size:        617     810    2850    2219

  Fragments arranged by size: 

                2850    2219     810     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4287     493 
   Size:        711    3083    2702

  Fragments arranged by size: 

                3083    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6490    6490 
   Size:       6496
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4303    4685    5320     275 
   Size:         53      83     186     936    2770     382     635    1451

  Fragments arranged by size: 

                2770    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4929    5327    5601    5748    6159    4929 
   Size:        398     274     147     411    5266

  Fragments arranged by size: 

                5266     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3662    3692    1178 
   Size:         30     165      30    2259      30    3982

  Fragments arranged by size: 

                3982    2259     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3450    1186 
   Size:       2264    4232
 
BseYI C'CCAG_C

Cuts at:   2144    2790    3573    5699    6195    2144 
   Size:        646     783    2126     496    2445

  Fragments arranged by size: 

                2445    2126     783     646     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5923
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3946    4707    5735    1540 
   Size:        275     186    1945     761    1028    2301

  Fragments arranged by size: 

                2301    1945    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2818    3756    4557    4642    5713    2818 
   Size:        938     801      85    1071    3601

  Fragments arranged by size: 

                3601    1071     938     801      85
 
BsmI GAATG_Cn'

Cuts at:   3557    4007    4100    3557 
   Size:        450      93    5953

  Fragments arranged by size: 

                5953     450      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2297    2818    3204    3756    3815    4557    4642 
   Size:        399     507     521     386     552      59     742      85

Cuts at:   4642    5713    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     742     552     521     507     399     386
                  85      59
 
BspEI T'CCGG_A

Cuts at:   6159    6159 
   Size:       6496
 
BspHI T'CATG_A

Cuts at:   3582    4385    6115    3582 
   Size:        803    1730    3963

  Fragments arranged by size: 

                3963    1730     803
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3851     844 
   Size:       3007    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2338    4383    1394 
   Size:        429     515    2045    3507

  Fragments arranged by size: 

                3507    2045     515     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4991    5165      65 
   Size:       1183    3743     174    1396

  Fragments arranged by size: 

                3743    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2059    5140    5327    1700 
   Size:        110     249    3081     187    2869

  Fragments arranged by size: 

                3081    2869     249     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6496
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6496
 
BssSI C'ACGA_G

Cuts at:   4556    5568    4556 
   Size:       1012    5484
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2462     794 
   Size:       1668    4828
 
BstBI TT'CG_AA

Cuts at:   5300    5300 
   Size:       6496
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6496
 
BstYI r'GATC_y

Cuts at:   1972    2019    2998    3826    4193    4578    4595    6047    6133 
   Size:         47     979     828     367     385      17    1452      86

Cuts at:   6133    6145    6491    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     979     828     385     367     346      86
                  47      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2027    2829    3630     513 
   Size:        552     939      23     802     801    3379

  Fragments arranged by size: 

                3379     939     802     801     552      23
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5757

  Fragments arranged by size: 

                5757     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3155    3624    4012    4806    4826    3155 
   Size:        469     388     794      20    4825

  Fragments arranged by size: 

                4825     794     469     388      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6496
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2550    2585     529 
   Size:         35    1986      35    4440

  Fragments arranged by size: 

                4440    1986      35      35
 
DraI TTT'AAA

Cuts at:   3800    3800 
   Size:       6496
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5503     809 
   Size:        507    4187    1802

  Fragments arranged by size: 

                4187    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2053    3943    4832 
   Size:         54    1425      50     275      18     223    1890     889

Cuts at:   4832    6461       8 
   Size:       1629      43

  Fragments arranged by size: 

                1890    1629    1425     889     275     223      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3943    1537 
   Size:        275    2131    4090

  Fragments arranged by size: 

                4090    2131     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4426    1353 
   Size:       3073    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    5083    5457    5603     387 
   Size:       1064     138    3494     374     146    1280

  Fragments arranged by size: 

                3494    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   3754    3754 
   Size:       6496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2105    2145    2255    2832    2882    4559    5156 
   Size:        393     473      40     110     577      50    1677     597

Cuts at:   5156    5943    6211    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1677    1524     787     597     577     473     393     268
                 110      50      40
 
EcoRI G'AATT_C

Cuts at:   3397    3805    4183    3397 
   Size:        408     378    5710

  Fragments arranged by size: 

                5710     408     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2670    3464    2670 
   Size:        794    5702
 
FspI TGC'GCA

Cuts at:   5002    5002 
   Size:       6496
 
HaeII r_GCGC'y

Cuts at:   1678    2694    5643    1678 
   Size:       1016    2949    2531

  Fragments arranged by size: 

                2949    2531    1016
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    5137    5169    5211    5243    1373 
   Size:         32     135      32    3565      32      42      32    2626

  Fragments arranged by size: 

                3565    2626     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3840    4086     669 
   Size:        988     281    1902     246    3079

  Fragments arranged by size: 

                3079    1902     988     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       6496
 
HpaI GTT'AAC

Cuts at:   4086    4086 
   Size:       6496
 
KpnI G_GTAC'C

Cuts at:   3821    3821 
   Size:       6496
 
MfeI C'AATT_G

Cuts at:   4095    4095 
   Size:       6496
 
MluI A'CGCG_T

Cuts at:   6372    6372 
   Size:       6496
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3100    3457    5610    5794    1305 
   Size:       1795     357    2153     184    2007

  Fragments arranged by size: 

                2153    2007    1795     357     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4674

  Fragments arranged by size: 

                4674    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3629    3635    4454    4813    4972     518 
   Size:        648    2463       6     819     359     159    2042

  Fragments arranged by size: 

                2463    2042     819     648     359     159       6
 
MspA1I CmG'CkG

Cuts at:   1606    2029    3573    3912    4589    5737    5982    1606 
   Size:        423    1544     339     677    1148     245    2120

  Fragments arranged by size: 

                2120    1544    1148     677     423     339     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2829    3630     513 
   Size:        552     939     825     801    3379

  Fragments arranged by size: 

                3379     939     825     801     552
 
NdeI CA'TA_TG

Cuts at:    387    2414     387 
   Size:       2027    4469
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6496
 
NotI GC'GGCC_GC

Cuts at:   3943    3943 
   Size:       6496
 
NsiI A_TGCA'T

Cuts at:   3012    3012 
   Size:       6496
 
NspI r_CATG'y

Cuts at:   1132    1229    2152    3854    5399    1132 
   Size:         97     923    1702    1545    2229

  Fragments arranged by size: 

                2229    1702    1545     923      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6496
 
PciI A'CATG_T

Cuts at:   5395    5395 
   Size:       6496
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2062    3313    1108 
   Size:        954    1251    4291

  Fragments arranged by size: 

                4291    1251     954
 
PmeI GTTT'AAAC

Cuts at:   3800    3800 
   Size:       6496
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4565    4597    6078    6110    4565 
   Size:         32    1481      32    4951

  Fragments arranged by size: 

                4951    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6496
 
PsiI TTA'TAA

Cuts at:   3170    4066    3170 
   Size:        896    5600
 
PstI C_TGCA'G

Cuts at:    830    3150    3706    3848     830 
   Size:       2320     556     142    3478

  Fragments arranged by size: 

                3478    2320     556     142
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6496
 
PvuII CAG'CTG

Cuts at:   3573    3573 
   Size:       6496
 
SacI G_AGCT'C

Cuts at:   3756    3756 
   Size:       6496
 
SacII CC_GC'GG

Cuts at:   2030    2030 
   Size:       6496
 
SalI G'TCGA_C

Cuts at:   1936    3838    1936 
   Size:       1902    4594
 
SbfI CC_TGCA'GG

Cuts at:   3848    3848 
   Size:       6496
 
ScaI AGT'ACT

Cuts at:   4744    6456    4744 
   Size:       1712    4784
 
SexAI A'CCwGG_T

Cuts at:   3352    3352 
   Size:       6496
 
SfcI C'TryA_G

Cuts at:    826    1045    3146    3702    3844    4979    5660    5851     826 
   Size:        219    2101     556     142    1135     681     191    1471

  Fragments arranged by size: 

                2101    1471    1135     681     556     219     191     142
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6496
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6496
 
SmaI CCC'GGG

Cuts at:   1872    2641    3823    1872 
   Size:        769    1182    4545

  Fragments arranged by size: 

                4545    1182     769
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2730    3711    4600    5501    5763     820 
   Size:        197     926     787     981     889     901     262    1553

  Fragments arranged by size: 

                1553     981     926     901     889     787     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    4287     493 
   Size:       3794    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6496
 
SphI G_CATG'C

Cuts at:   1132    2152    3854    1132 
   Size:       1020    1702    3774

  Fragments arranged by size: 

                3774    1702    1020
 
SspI AAT'ATT

Cuts at:      5      52    2845    4420       5 
   Size:         47    2793    1575    2081

  Fragments arranged by size: 

                2793    2081    1575      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6496
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2829    3630    3666    3935     513 
   Size:        552     939     825     801      36     269    3074

  Fragments arranged by size: 

                3074     939     825     801     552     269      36
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2244    4523    4540    4693    4878    5323     325 
   Size:       1255     664    2279      17     153     185     445    1498

  Fragments arranged by size: 

                2279    1498    1255     664     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2381    3260    3698 
   Size:        274      80      33      51    1443     404     879     438

Cuts at:   3698    4742    6454      96 
   Size:       1044    1712     138

  Fragments arranged by size: 

                1712    1443    1044     879     438     404     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6456    6488    6456 
   Size:         32    6464
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6496
 
XbaI T'CTAG_A

Cuts at:   3832    3832 
   Size:       6496
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3314    3314 
   Size:       6496
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6496
 
XmaI C'CCGG_G

Cuts at:   1870    2639    3821    1870 
   Size:        769    1182    4545

  Fragments arranged by size: 

                4545    1182     769
 
XmnI GAAnn'nnTTC

Cuts at:   2523    4625    2523 
   Size:       2102    4394
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4304     276 
   Size:         53      83     186     936    2770    2468

  Fragments arranged by size: 

                2770    2468     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NsiI     NspI     PasI
     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI     PvuI
    PvuII     SacI    SacII     SalI     SbfI     ScaI    SexAI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PsrI    RsrII    SanDI     SapI     SfiI     SfoI     SrfI     SwaI
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