(Circular) (Six-base) MAPSORT of: pFN21AB9518.seq Check: 7470 from: 1 to: 6496 pFN21AB9518 6496 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4306 278 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3839 1937 Size: 1902 4594 Acc65I G'GTAC_C Cuts at: 3817 3817 Size: 6496 AclI AA'CG_TT Cuts at: 1563 4623 4996 1563 Size: 3060 373 3063 Fragments arranged by size: 3063 3060 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2105 2145 2255 2882 4559 5943 6211 1632 Size: 473 40 110 627 1677 1384 268 1917 Fragments arranged by size: 1917 1677 1384 627 473 268 110 40 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6299 AflIII A'CryG_T Cuts at: 1203 5395 6372 1203 Size: 4192 977 1327 Fragments arranged by size: 4192 1327 977 AgeI A'CCGG_T Cuts at: 5327 5327 Size: 6496 AhdI GACnn_n'nnGTC Cuts at: 5225 5225 Size: 6496 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6496 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6496 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3054 3086 3054 Size: 32 6464 AlwNI CAG_nnn'CTG Cuts at: 4239 5811 4239 Size: 1572 4924 ApaLI G'TGCA_C Cuts at: 4553 5709 4553 Size: 1156 5340 ApoI r'AATT_y Cuts at: 1493 2981 3101 3286 3397 3557 3805 4031 4183 Size: 1488 120 185 111 160 248 226 152 Cuts at: 4183 6292 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1488 248 226 185 160 152 120 111 AseI AT'TA_AT Cuts at: 160 1032 5050 160 Size: 872 4018 1606 Fragments arranged by size: 4018 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 AvaI C'yCGr_G Cuts at: 1870 1943 2639 3821 1870 Size: 73 696 1182 4545 Fragments arranged by size: 4545 1182 696 73 BamHI G'GATC_C Cuts at: 3826 4193 3826 Size: 367 6129 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3817 5272 618 Size: 325 217 627 2030 1455 1842 Fragments arranged by size: 2030 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3204 3756 3815 1391 Size: 1813 552 59 4072 Fragments arranged by size: 4072 1813 552 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2093 2604 3200 3571 928 Size: 971 194 511 596 371 3853 Fragments arranged by size: 3853 971 596 511 371 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4687 4721 1903 Size: 34 2750 34 3678 Fragments arranged by size: 3678 2750 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4388 5598 4388 Size: 1210 5286 BclI T'GATC_A Cuts at: 1548 1548 Size: 6496 BfrBI ATG'CAT Cuts at: 3010 3010 Size: 6496 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2032 5107 136 Size: 107 122 71 631 605 360 3075 1525 Fragments arranged by size: 3075 1525 631 605 360 122 107 71 BglII A'GATC_T Cuts at: 2998 6491 2998 Size: 3493 3003 BlpI GC'TnA_GC Cuts at: 3913 3913 Size: 6496 Bme1580I G_kGCm'C Cuts at: 1790 2297 4557 5713 1790 Size: 507 2260 1156 2573 Fragments arranged by size: 2573 2260 1156 507 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6496 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2696 3207 3384 Size: 416 53 83 399 24 1279 511 177 Cuts at: 3384 4227 5185 442 Size: 843 958 1753 Fragments arranged by size: 1753 1279 958 843 511 416 399 177 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6496 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2832 5156 1239 Size: 1593 2324 2579 Fragments arranged by size: 2579 2324 1593 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2715 3696 4621 5486 5784 2715 Size: 981 925 865 298 3427 Fragments arranged by size: 3427 981 925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2309 5159 882 Size: 617 810 2850 2219 Fragments arranged by size: 2850 2219 810 617 BsaAI yAC'GTr Cuts at: 493 1204 4287 493 Size: 711 3083 2702 Fragments arranged by size: 3083 2702 711 BsaBI GATnn'nnATC Cuts at: 6490 6490 Size: 6496 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4303 4685 5320 275 Size: 53 83 186 936 2770 382 635 1451 Fragments arranged by size: 2770 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4929 5327 5601 5748 6159 4929 Size: 398 274 147 411 5266 Fragments arranged by size: 5266 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3662 3692 1178 Size: 30 165 30 2259 30 3982 Fragments arranged by size: 3982 2259 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3450 1186 Size: 2264 4232 BseYI C'CCAG_C Cuts at: 2144 2790 3573 5699 6195 2144 Size: 646 783 2126 496 2445 Fragments arranged by size: 2445 2126 783 646 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5923 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3946 4707 5735 1540 Size: 275 186 1945 761 1028 2301 Fragments arranged by size: 2301 1945 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2818 3756 4557 4642 5713 2818 Size: 938 801 85 1071 3601 Fragments arranged by size: 3601 1071 938 801 85 BsmI GAATG_Cn' Cuts at: 3557 4007 4100 3557 Size: 450 93 5953 Fragments arranged by size: 5953 450 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2297 2818 3204 3756 3815 4557 4642 Size: 399 507 521 386 552 59 742 85 Cuts at: 4642 5713 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 552 521 507 399 386 85 59 BspEI T'CCGG_A Cuts at: 6159 6159 Size: 6496 BspHI T'CATG_A Cuts at: 3582 4385 6115 3582 Size: 803 1730 3963 Fragments arranged by size: 3963 1730 803 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3851 844 Size: 3007 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2338 4383 1394 Size: 429 515 2045 3507 Fragments arranged by size: 3507 2045 515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4991 5165 65 Size: 1183 3743 174 1396 Fragments arranged by size: 3743 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 5140 5327 1700 Size: 110 249 3081 187 2869 Fragments arranged by size: 3081 2869 249 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6496 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6496 BssSI C'ACGA_G Cuts at: 4556 5568 4556 Size: 1012 5484 BstAPI GCAn_nnn'nTGC Cuts at: 794 2462 794 Size: 1668 4828 BstBI TT'CG_AA Cuts at: 5300 5300 Size: 6496 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6496 BstYI r'GATC_y Cuts at: 1972 2019 2998 3826 4193 4578 4595 6047 6133 Size: 47 979 828 367 385 17 1452 86 Cuts at: 6133 6145 6491 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 979 828 385 367 346 86 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2027 2829 3630 513 Size: 552 939 23 802 801 3379 Fragments arranged by size: 3379 939 802 801 552 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5757 Fragments arranged by size: 5757 683 56 BtsI GCAGTG_nn' Cuts at: 3155 3624 4012 4806 4826 3155 Size: 469 388 794 20 4825 Fragments arranged by size: 4825 794 469 388 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6496 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2550 2585 529 Size: 35 1986 35 4440 Fragments arranged by size: 4440 1986 35 35 DraI TTT'AAA Cuts at: 3800 3800 Size: 6496 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5503 809 Size: 507 4187 1802 Fragments arranged by size: 4187 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 3943 4832 Size: 54 1425 50 275 18 223 1890 889 Cuts at: 4832 6461 8 Size: 1629 43 Fragments arranged by size: 1890 1629 1425 889 275 223 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3943 1537 Size: 275 2131 4090 Fragments arranged by size: 4090 2131 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4426 1353 Size: 3073 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5083 5457 5603 387 Size: 1064 138 3494 374 146 1280 Fragments arranged by size: 3494 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3754 3754 Size: 6496 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2145 2255 2832 2882 4559 5156 Size: 393 473 40 110 577 50 1677 597 Cuts at: 5156 5943 6211 1239 Size: 787 268 1524 Fragments arranged by size: 1677 1524 787 597 577 473 393 268 110 50 40 EcoRI G'AATT_C Cuts at: 3397 3805 4183 3397 Size: 408 378 5710 Fragments arranged by size: 5710 408 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2670 3464 2670 Size: 794 5702 FspI TGC'GCA Cuts at: 5002 5002 Size: 6496 HaeII r_GCGC'y Cuts at: 1678 2694 5643 1678 Size: 1016 2949 2531 Fragments arranged by size: 2949 2531 1016 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5137 5169 5211 5243 1373 Size: 32 135 32 3565 32 42 32 2626 Fragments arranged by size: 3565 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3840 4086 669 Size: 988 281 1902 246 3079 Fragments arranged by size: 3079 1902 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6496 HpaI GTT'AAC Cuts at: 4086 4086 Size: 6496 KpnI G_GTAC'C Cuts at: 3821 3821 Size: 6496 MfeI C'AATT_G Cuts at: 4095 4095 Size: 6496 MluI A'CGCG_T Cuts at: 6372 6372 Size: 6496 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3100 3457 5610 5794 1305 Size: 1795 357 2153 184 2007 Fragments arranged by size: 2153 2007 1795 357 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4674 Fragments arranged by size: 4674 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3629 3635 4454 4813 4972 518 Size: 648 2463 6 819 359 159 2042 Fragments arranged by size: 2463 2042 819 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2029 3573 3912 4589 5737 5982 1606 Size: 423 1544 339 677 1148 245 2120 Fragments arranged by size: 2120 1544 1148 677 423 339 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2829 3630 513 Size: 552 939 825 801 3379 Fragments arranged by size: 3379 939 825 801 552 NdeI CA'TA_TG Cuts at: 387 2414 387 Size: 2027 4469 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6496 NotI GC'GGCC_GC Cuts at: 3943 3943 Size: 6496 NsiI A_TGCA'T Cuts at: 3012 3012 Size: 6496 NspI r_CATG'y Cuts at: 1132 1229 2152 3854 5399 1132 Size: 97 923 1702 1545 2229 Fragments arranged by size: 2229 1702 1545 923 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6496 PciI A'CATG_T Cuts at: 5395 5395 Size: 6496 PflMI CCAn_nnn'nTGG Cuts at: 1108 2062 3313 1108 Size: 954 1251 4291 Fragments arranged by size: 4291 1251 954 PmeI GTTT'AAAC Cuts at: 3800 3800 Size: 6496 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4565 4597 6078 6110 4565 Size: 32 1481 32 4951 Fragments arranged by size: 4951 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6496 PsiI TTA'TAA Cuts at: 3170 4066 3170 Size: 896 5600 PstI C_TGCA'G Cuts at: 830 3150 3706 3848 830 Size: 2320 556 142 3478 Fragments arranged by size: 3478 2320 556 142 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6496 PvuII CAG'CTG Cuts at: 3573 3573 Size: 6496 SacI G_AGCT'C Cuts at: 3756 3756 Size: 6496 SacII CC_GC'GG Cuts at: 2030 2030 Size: 6496 SalI G'TCGA_C Cuts at: 1936 3838 1936 Size: 1902 4594 SbfI CC_TGCA'GG Cuts at: 3848 3848 Size: 6496 ScaI AGT'ACT Cuts at: 4744 6456 4744 Size: 1712 4784 SexAI A'CCwGG_T Cuts at: 3352 3352 Size: 6496 SfcI C'TryA_G Cuts at: 826 1045 3146 3702 3844 4979 5660 5851 826 Size: 219 2101 556 142 1135 681 191 1471 Fragments arranged by size: 2101 1471 1135 681 556 219 191 142 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6496 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6496 SmaI CCC'GGG Cuts at: 1872 2641 3823 1872 Size: 769 1182 4545 Fragments arranged by size: 4545 1182 769 SmlI C'TyrA_G Cuts at: 820 1017 1943 2730 3711 4600 5501 5763 820 Size: 197 926 787 981 889 901 262 1553 Fragments arranged by size: 1553 981 926 901 889 787 262 197 SnaBI TAC'GTA Cuts at: 493 4287 493 Size: 3794 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6496 SphI G_CATG'C Cuts at: 1132 2152 3854 1132 Size: 1020 1702 3774 Fragments arranged by size: 3774 1702 1020 SspI AAT'ATT Cuts at: 5 52 2845 4420 5 Size: 47 2793 1575 2081 Fragments arranged by size: 2793 2081 1575 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6496 StyI C'CwwG_G Cuts at: 513 1065 2004 2829 3630 3666 3935 513 Size: 552 939 825 801 36 269 3074 Fragments arranged by size: 3074 939 825 801 552 269 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2244 4523 4540 4693 4878 5323 325 Size: 1255 664 2279 17 153 185 445 1498 Fragments arranged by size: 2279 1498 1255 664 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2381 3260 3698 Size: 274 80 33 51 1443 404 879 438 Cuts at: 3698 4742 6454 96 Size: 1044 1712 138 Fragments arranged by size: 1712 1443 1044 879 438 404 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6456 6488 6456 Size: 32 6464 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6496 XbaI T'CTAG_A Cuts at: 3832 3832 Size: 6496 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3314 3314 Size: 6496 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6496 XmaI C'CCGG_G Cuts at: 1870 2639 3821 1870 Size: 769 1182 4545 Fragments arranged by size: 4545 1182 769 XmnI GAAnn'nnTTC Cuts at: 2523 4625 2523 Size: 2102 4394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4304 276 Size: 53 83 186 936 2770 2468 Fragments arranged by size: 2770 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI