Restriction map for
pFN21AB9518
(Linear) (Six-base) MAPSORT of: KIBB9518.seq Check: 6875 from: 1 to: 1791
KIBB9518.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 140 250 877 1791
Size: 100 40 110 627 914
Fragments arranged by size:
914 627 110 100 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1049 1081 1791
Size: 1049 32 710
Fragments arranged by size:
1049 710 32
ApoI r'AATT_y
Cuts at: 0 976 1096 1281 1392 1552 1791
Size: 976 120 185 111 160 239
Fragments arranged by size:
976 239 185 160 120 111
AvaI C'yCGr_G
Cuts at: 0 634 1791
Size: 634 1157
BanII G_rGCy'C
Cuts at: 0 1199 1751 1791
Size: 1199 552 40
Fragments arranged by size:
1199 552 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 88 599 1195 1566 1791
Size: 88 511 596 371 225
Fragments arranged by size:
596 511 371 225 88
BfrBI ATG'CAT
Cuts at: 0 1005 1791
Size: 1005 786
BglI GCCn_nnn'nGGC
Cuts at: 0 27 1791
Size: 27 1764
BglII A'GATC_T
Cuts at: 0 993 1791
Size: 993 798
Bme1580I G_kGCm'C
Cuts at: 0 292 1791
Size: 292 1499
BmrI ACTGGGnnnn_n'
Cuts at: 0 691 1202 1379 1791
Size: 691 511 177 412
Fragments arranged by size:
691 511 412 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1791
Size: 827 964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1691 1791
Size: 710 981 100
Fragments arranged by size:
981 710 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 304 1791
Size: 304 1487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1657 1687 1791
Size: 1657 30 104
Fragments arranged by size:
1657 104 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1445 1791
Size: 1445 346
BseYI C'CCAG_C
Cuts at: 0 139 785 1568 1791
Size: 139 646 783 223
Fragments arranged by size:
783 646 223 139
BsiHKAI G_wGCw'C
Cuts at: 0 813 1751 1791
Size: 813 938 40
Fragments arranged by size:
938 813 40
BsmI GAATG_Cn'
Cuts at: 0 1552 1791
Size: 1552 239
Bsp1286I G_dGCh'C
Cuts at: 0 292 813 1199 1751 1791
Size: 292 521 386 552 40
Fragments arranged by size:
552 521 386 292 40
BspHI T'CATG_A
Cuts at: 0 1577 1791
Size: 1577 214
BsrBI CCG'CTC
Cuts at: 0 333 1791
Size: 333 1458
BsrFI r'CCGG_y
Cuts at: 0 54 1791
Size: 54 1737
BstAPI GCAn_nnn'nTGC
Cuts at: 0 457 1791
Size: 457 1334
BstYI r'GATC_y
Cuts at: 0 14 993 1791
Size: 14 979 798
Fragments arranged by size:
979 798 14
BtgI C'CryG_G
Cuts at: 0 22 824 1625 1791
Size: 22 802 801 166
Fragments arranged by size:
802 801 166 22
BtsI GCAGTG_nn'
Cuts at: 0 1150 1619 1791
Size: 1150 469 172
Fragments arranged by size:
1150 469 172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 545 580 1791
Size: 545 35 1211
Fragments arranged by size:
1211 545 35
EaeI y'GGCC_r
Cuts at: 0 48 1791
Size: 48 1743
EcoICRI GAG'CTC
Cuts at: 0 1749 1791
Size: 1749 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 140 250 827 877 1791
Size: 100 40 110 577 50 914
Fragments arranged by size:
914 577 110 100 50 40
EcoRI G'AATT_C
Cuts at: 0 1392 1791
Size: 1392 399
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 665 1459 1791
Size: 665 794 332
Fragments arranged by size:
794 665 332
HaeII r_GCGC'y
Cuts at: 0 689 1791
Size: 689 1102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1095 1452 1791
Size: 1095 357 339
Fragments arranged by size:
1095 357 339
MslI CAynn'nnrTG
Cuts at: 0 1624 1630 1791
Size: 1624 6 161
Fragments arranged by size:
1624 161 6
MspA1I CmG'CkG
Cuts at: 0 24 1568 1791
Size: 24 1544 223
Fragments arranged by size:
1544 223 24
NcoI C'CATG_G
Cuts at: 0 824 1625 1791
Size: 824 801 166
Fragments arranged by size:
824 801 166
NdeI CA'TA_TG
Cuts at: 0 409 1791
Size: 409 1382
NsiI A_TGCA'T
Cuts at: 0 1007 1791
Size: 1007 784
NspI r_CATG'y
Cuts at: 0 147 1791
Size: 147 1644
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 1308 1791
Size: 57 1251 483
Fragments arranged by size:
1251 483 57
PsiI TTA'TAA
Cuts at: 0 1165 1791
Size: 1165 626
PstI C_TGCA'G
Cuts at: 0 1145 1701 1791
Size: 1145 556 90
Fragments arranged by size:
1145 556 90
PvuII CAG'CTG
Cuts at: 0 1568 1791
Size: 1568 223
SacI G_AGCT'C
Cuts at: 0 1751 1791
Size: 1751 40
SacII CC_GC'GG
Cuts at: 0 25 1791
Size: 25 1766
SexAI A'CCwGG_T
Cuts at: 0 1347 1791
Size: 1347 444
SfcI C'TryA_G
Cuts at: 0 1141 1697 1791
Size: 1141 556 94
Fragments arranged by size:
1141 556 94
SmaI CCC'GGG
Cuts at: 0 636 1791
Size: 636 1155
SmlI C'TyrA_G
Cuts at: 0 725 1706 1791
Size: 725 981 85
Fragments arranged by size:
981 725 85
SphI G_CATG'C
Cuts at: 0 147 1791
Size: 147 1644
SspI AAT'ATT
Cuts at: 0 840 1791
Size: 840 951
StyI C'CwwG_G
Cuts at: 0 824 1625 1661 1791
Size: 824 801 36 130
Fragments arranged by size:
824 801 130 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 1791
Size: 239 1552
TatI w'GTAC_w
Cuts at: 0 376 1255 1693 1791
Size: 376 879 438 98
Fragments arranged by size:
879 438 376 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1309 1791
Size: 1309 482
XmaI C'CCGG_G
Cuts at: 0 634 1791
Size: 634 1157
XmnI GAAnn'nnTTC
Cuts at: 0 518 1791
Size: 518 1273
Enzymes that do cut:
AcuI AloI ApoI AvaI BanII BbsI BfrBI BglI
BglII Bme1580I BmrI BpmI BpuEI BsaI BsaXI BseRI
BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI BstAPI
BstYI BtgI BtsI CspCI EaeI EcoICRI Eco57MI EcoRI
FalI HaeII MmeI MslI MspA1I NcoI NdeI NsiI
NspI PflMI PsiI PstI PvuII SacI SacII SexAI
SfcI SmaI SmlI SphI SspI StyI TaqII TatI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BlpI BmgBI BmtI BplI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmBI
BspEI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EarI EciI EcoNI EcoO109I EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XhoI
ZraI