(Circular) (Six-base) MAPSORT of: pFN21AB9517.seq Check: 9981 from: 1 to: 6400 pFN21AB9517 6400 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3241 3241 Size: 6400 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3743 1937 Size: 1806 4594 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 4527 4900 1563 Size: 2964 373 3063 Fragments arranged by size: 3063 2964 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 3377 3599 4463 5847 6115 1632 Size: 531 1214 222 864 1384 268 1917 Fragments arranged by size: 1917 1384 1214 864 531 268 222 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6203 AflIII A'CryG_T Cuts at: 1203 2076 2760 5299 6276 1203 Size: 873 684 2539 977 1327 Fragments arranged by size: 2539 1327 977 873 684 AgeI A'CCGG_T Cuts at: 2982 5231 2982 Size: 2249 4151 AhdI GACnn_n'nnGTC Cuts at: 3131 5129 3131 Size: 1998 4402 AleI CACnn'nnGTG Cuts at: 1166 2486 3492 1166 Size: 1320 1006 4074 Fragments arranged by size: 4074 1320 1006 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6400 AlwNI CAG_nnn'CTG Cuts at: 2293 3041 3537 3550 4143 5715 2293 Size: 748 496 13 593 1572 2978 Fragments arranged by size: 2978 1572 748 593 496 13 ApaI G_GGCC'C Cuts at: 2071 3409 3465 2071 Size: 1338 56 5006 Fragments arranged by size: 5006 1338 56 ApaLI G'TGCA_C Cuts at: 2764 3647 4457 5613 2764 Size: 883 810 1156 3551 Fragments arranged by size: 3551 1156 883 810 ApoI r'AATT_y Cuts at: 1493 2912 3709 3935 4087 6196 1493 Size: 1419 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 1419 797 226 152 AseI AT'TA_AT Cuts at: 160 1032 4954 160 Size: 872 3922 1606 Fragments arranged by size: 3922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 2226 2639 3725 1870 Size: 73 283 413 1086 4545 Fragments arranged by size: 4545 1086 413 283 73 BamHI G'GATC_C Cuts at: 2941 3730 4097 2941 Size: 789 367 5244 Fragments arranged by size: 5244 789 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2493 2547 2635 3721 5176 Size: 325 217 627 706 54 88 1086 1455 Cuts at: 5176 618 Size: 1842 Fragments arranged by size: 1842 1455 1086 706 627 325 217 88 54 BanII G_rGCy'C Cuts at: 1391 2071 2151 3409 3465 3719 1391 Size: 680 80 1258 56 254 4072 Fragments arranged by size: 4072 1258 680 254 80 56 BbeI G_GCGC'C Cuts at: 2551 2551 Size: 6400 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3398 3587 928 Size: 971 1499 189 3741 Fragments arranged by size: 3741 1499 971 189 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4591 4625 1903 Size: 34 2654 34 3678 Fragments arranged by size: 3678 2654 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 5502 4292 Size: 1210 5190 BclI T'GATC_A Cuts at: 1548 3195 1548 Size: 1647 4753 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5011 136 Size: 107 122 71 631 605 3339 1525 Fragments arranged by size: 3339 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6395 6395 Size: 6400 BlpI GC'TnA_GC Cuts at: 2840 3817 2840 Size: 977 5423 Bme1580I G_kGCm'C Cuts at: 1790 2071 2640 2768 3409 3465 3475 3651 4461 Size: 281 569 128 641 56 10 176 810 Cuts at: 4461 5617 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 810 641 569 281 176 128 56 10 BmgBI CAC'GTC Cuts at: 1318 2424 3655 1318 Size: 1106 1231 4063 Fragments arranged by size: 4063 1231 1106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2430 4131 5089 Size: 416 53 83 399 24 1013 1701 958 Cuts at: 5089 442 Size: 1753 Fragments arranged by size: 1753 1701 1013 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6400 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 5060 1239 Size: 896 2925 2579 Fragments arranged by size: 2925 2579 896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3314 4525 5390 5688 3314 Size: 1211 865 298 4026 Fragments arranged by size: 4026 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2108 5063 882 Size: 617 609 2955 2219 Fragments arranged by size: 2955 2219 617 609 BsaAI yAC'GTr Cuts at: 493 1204 2813 3588 4191 493 Size: 711 1609 775 603 2702 Fragments arranged by size: 2702 1609 775 711 603 BsaBI GATnn'nnATC Cuts at: 2669 6394 2669 Size: 3725 2675 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2548 4207 4589 5224 Size: 53 83 186 936 1015 1659 382 635 Cuts at: 5224 275 Size: 1451 Fragments arranged by size: 1659 1451 1015 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 4833 5231 5505 5652 6063 2982 Size: 1851 398 274 147 411 3319 Fragments arranged by size: 3319 1851 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2088 2118 2348 2378 3092 Size: 30 165 30 685 30 230 30 714 Cuts at: 3092 3122 1178 Size: 30 4456 Fragments arranged by size: 4456 714 685 230 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 2356 2612 2743 2798 2831 3113 3224 Size: 989 181 256 131 55 33 282 111 Cuts at: 3224 3260 3359 3455 1186 Size: 36 99 96 4131 Fragments arranged by size: 4131 989 282 256 181 131 111 99 96 55 36 33 BseYI C'CCAG_C Cuts at: 2109 2234 3450 5603 6099 2109 Size: 125 1216 2153 496 2410 Fragments arranged by size: 2410 2153 1216 496 125 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3302 3404 3516 1163 Size: 573 1566 102 112 4047 Fragments arranged by size: 4047 1566 573 112 102 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3144 3850 4611 5639 1540 Size: 275 186 1143 706 761 1028 2301 Fragments arranged by size: 2301 1143 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 2313 2768 3177 3651 4461 4546 5617 2313 Size: 455 409 474 810 85 1071 3096 Fragments arranged by size: 3096 1071 810 474 455 409 85 BsiWI C'GTAC_G Cuts at: 3258 3258 Size: 6400 BsmI GAATG_Cn' Cuts at: 3911 4004 3911 Size: 93 6307 BsmBI CGTCTCn'nnnn_ Cuts at: 2368 2368 Size: 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2151 2313 2640 2768 3177 3409 Size: 399 281 80 162 327 128 409 232 Cuts at: 3409 3465 3475 3651 3719 4461 4546 5617 1391 Size: 56 10 176 68 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 327 281 232 176 162 128 85 80 68 56 10 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 2875 3192 3657 4289 6019 2875 Size: 317 465 632 1730 3256 Fragments arranged by size: 3256 1730 632 465 317 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2844 3241 3755 844 Size: 2000 397 514 3489 Fragments arranged by size: 3489 2000 514 397 BsrBI CCG'CTC Cuts at: 1394 1823 4287 1394 Size: 429 2464 3507 Fragments arranged by size: 3507 2464 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3522 4895 5069 65 Size: 1183 2274 1373 174 1396 Fragments arranged by size: 2274 1396 1373 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2982 3309 3512 5044 5231 1700 Size: 110 1172 327 203 1532 187 2869 Fragments arranged by size: 2869 1532 1172 327 203 187 110 BsrGI T'GTAC_A Cuts at: 96 3108 96 Size: 3012 3388 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6400 BssSI C'ACGA_G Cuts at: 4460 5472 4460 Size: 1012 5388 BstAPI GCAn_nnn'nTGC Cuts at: 794 2268 3427 3540 794 Size: 1474 1159 113 3654 Fragments arranged by size: 3654 1474 1159 113 BstBI TT'CG_AA Cuts at: 5204 5204 Size: 6400 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6400 BstYI r'GATC_y Cuts at: 1972 2203 2941 3730 4097 4482 4499 5951 6037 Size: 231 738 789 367 385 17 1452 86 Cuts at: 6037 6049 6395 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 789 738 385 367 346 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2501 2538 2679 2501 Size: 37 141 6222 Fragments arranged by size: 6222 141 37 BtgI C'CryG_G Cuts at: 513 1065 2400 2460 2712 513 Size: 552 1335 60 252 4201 Fragments arranged by size: 4201 1335 552 252 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3097 487 Size: 683 56 1871 3790 Fragments arranged by size: 3790 1871 683 56 BtsI GCAGTG_nn' Cuts at: 2092 2528 3487 3499 3916 4710 4730 2092 Size: 436 959 12 417 794 20 3762 Fragments arranged by size: 3762 959 794 436 417 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6400 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6365 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DraIII CAC_nnn'GTG Cuts at: 3427 3427 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5407 809 Size: 507 4091 1802 Fragments arranged by size: 4091 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2329 3239 3847 Size: 54 1425 50 275 18 499 910 608 Cuts at: 3847 4736 6365 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 910 889 608 499 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3847 1537 Size: 275 2035 4090 Fragments arranged by size: 4090 2035 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2862 4330 1353 Size: 1509 1468 3423 Fragments arranged by size: 3423 1509 1468 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2812 2924 4987 5361 5507 387 Size: 1064 138 1223 112 2063 374 146 1280 Fragments arranged by size: 2063 1280 1223 1064 374 146 138 112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2163 3377 3599 4463 5060 5847 Size: 393 503 28 1214 222 864 597 787 Cuts at: 5847 6115 1239 Size: 268 1524 Fragments arranged by size: 1524 1214 864 787 597 503 393 268 222 28 EcoNI CCTnn'n_nnAGG Cuts at: 2903 2903 Size: 6400 EcoO109I rG'GnC_Cy Cuts at: 2067 2068 2884 3125 3406 3461 3462 2067 Size: 1 816 241 281 55 1 5005 Fragments arranged by size: 5005 816 281 241 55 1 1 EcoRI G'AATT_C Cuts at: 2912 3709 4087 2912 Size: 797 378 5225 Fragments arranged by size: 5225 797 378 FspI TGC'GCA Cuts at: 4906 4906 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 2235 2551 2847 5547 1678 Size: 557 316 296 2700 2531 Fragments arranged by size: 2700 2531 557 316 296 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2678 2710 3117 3149 5041 Size: 32 135 32 1106 32 407 32 1892 Cuts at: 5041 5073 5115 5147 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1892 1106 407 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3744 3990 669 Size: 988 281 1806 246 3079 Fragments arranged by size: 3079 1806 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6400 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KasI G'GCGC_C Cuts at: 2547 2547 Size: 6400 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 6276 6276 Size: 6400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5514 5698 1305 Size: 4209 184 2007 Fragments arranged by size: 4209 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2331 10 Size: 54 1425 343 499 4079 Fragments arranged by size: 4079 1425 499 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2486 3191 3492 4358 4717 4876 518 Size: 648 1320 705 301 866 359 159 2042 Fragments arranged by size: 2042 1320 866 705 648 359 301 159 MspA1I CmG'CkG Cuts at: 1606 2322 2392 3550 3679 3816 4493 5641 5886 Size: 716 70 1158 129 137 677 1148 245 Cuts at: 5886 1606 Size: 2120 Fragments arranged by size: 2120 1158 1148 716 677 245 137 129 70 NarI GG'CG_CC Cuts at: 2548 2548 Size: 6400 NcoI C'CATG_G Cuts at: 513 1065 2712 513 Size: 552 1647 4201 Fragments arranged by size: 4201 1647 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6400 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6400 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 2080 2764 3630 3758 5303 1132 Size: 97 851 684 866 128 1545 2229 Fragments arranged by size: 2229 1545 866 851 684 128 97 PasI CC'CwG_GG Cuts at: 1348 2963 1348 Size: 1615 4785 PciI A'CATG_T Cuts at: 2076 2760 5299 2076 Size: 684 2539 3177 Fragments arranged by size: 3177 2539 684 PflMI CCAn_nnn'nTGG Cuts at: 1108 2406 1108 Size: 1298 5102 PfoI T'CCnGG_A Cuts at: 2151 2619 2151 Size: 468 5932 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PmlI CAC'GTG Cuts at: 2813 3588 2813 Size: 775 5625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2719 2751 4469 4501 5982 6014 2719 Size: 32 1718 32 1481 32 3105 Fragments arranged by size: 3105 1718 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3125 3125 Size: 6400 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 3970 3970 Size: 6400 PspOMI G'GGCC_C Cuts at: 2067 3405 3461 2067 Size: 1338 56 5006 Fragments arranged by size: 5006 1338 56 PspXI vC'TCGA_Gb Cuts at: 2226 2226 Size: 6400 PstI C_TGCA'G Cuts at: 830 2087 2724 2907 3494 3752 830 Size: 1257 637 183 587 258 3478 Fragments arranged by size: 3478 1257 637 587 258 183 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 PvuII CAG'CTG Cuts at: 2392 3550 3679 2392 Size: 1158 129 5113 Fragments arranged by size: 5113 1158 129 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SanDI GG'GwC_CC Cuts at: 3125 3125 Size: 6400 SapI GCTCTTCn'nnn_ Cuts at: 2862 2862 Size: 6400 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 4648 6360 4648 Size: 1712 4688 SexAI A'CCwGG_T Cuts at: 3423 3423 Size: 6400 SfcI C'TryA_G Cuts at: 826 1045 2083 2720 2903 3490 3748 4883 5564 Size: 219 1038 637 183 587 258 1135 681 Cuts at: 5564 5755 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1038 681 637 587 258 219 191 183 SfoI GGC'GCC Cuts at: 2549 2549 Size: 6400 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 3727 1872 Size: 1855 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2226 3329 4504 5405 5667 820 Size: 197 926 283 1103 1175 901 262 1553 Fragments arranged by size: 1553 1175 1103 926 901 283 262 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SspI AAT'ATT Cuts at: 5 52 4324 5 Size: 47 4272 2081 Fragments arranged by size: 4272 2081 47 StuI AGG'CCT Cuts at: 1517 3354 1517 Size: 1837 4563 StyI C'CwwG_G Cuts at: 513 1065 2712 3839 513 Size: 552 1647 1127 3074 Fragments arranged by size: 3074 1647 1127 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2536 4427 4444 4597 4782 5227 325 Size: 1255 956 1891 17 153 185 445 1498 Fragments arranged by size: 1891 1498 1255 956 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3108 4646 6358 Size: 274 80 33 51 1443 1131 1538 1712 Cuts at: 6358 96 Size: 138 Fragments arranged by size: 1712 1538 1443 1131 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6360 6392 6360 Size: 32 6368 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6400 XbaI T'CTAG_A Cuts at: 3736 3736 Size: 6400 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2443 2935 2443 Size: 492 5908 XhoI C'TCGA_G Cuts at: 1943 2226 1943 Size: 283 6117 XmaI C'CCGG_G Cuts at: 1870 3725 1870 Size: 1855 4545 XmnI GAAnn'nnTTC Cuts at: 2871 4529 2871 Size: 1658 4742 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacI SacII SfiI SrfI SwaI