(Circular) (Six-base) MAPSORT of: pFN21AB9515.seq Check: 3997 from: 1 to: 6238 pFN21AB9515 6238 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2677 2677 Size: 6238 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4048 278 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2039 2464 2946 3581 1937 Size: 102 425 482 635 4594 Fragments arranged by size: 4594 635 482 425 102 Acc65I G'GTAC_C Cuts at: 2865 3559 2865 Size: 694 5544 AclI AA'CG_TT Cuts at: 1563 4365 4738 1563 Size: 2802 373 3063 Fragments arranged by size: 3063 2802 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2362 4301 5685 5953 1632 Size: 730 1939 1384 268 1917 Fragments arranged by size: 1939 1917 1384 730 268 AfeI AGC'GCT Cuts at: 3343 3343 Size: 6238 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6041 AflIII A'CryG_T Cuts at: 1203 2521 5137 6114 1203 Size: 1318 2616 977 1327 Fragments arranged by size: 2616 1327 1318 977 AgeI A'CCGG_T Cuts at: 5069 5069 Size: 6238 AhdI GACnn_n'nnGTC Cuts at: 4967 4967 Size: 6238 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6238 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6238 AlwNI CAG_nnn'CTG Cuts at: 2261 2590 3981 5553 2261 Size: 329 1391 1572 2946 Fragments arranged by size: 2946 1572 1391 329 ApaLI G'TGCA_C Cuts at: 4295 5451 4295 Size: 1156 5082 ApoI r'AATT_y Cuts at: 1493 2233 2415 2804 3037 3547 3773 3925 6034 Size: 740 182 389 233 510 226 152 2109 Cuts at: 6034 1493 Size: 1697 Fragments arranged by size: 2109 1697 740 510 389 233 226 182 152 AseI AT'TA_AT Cuts at: 160 1032 3411 4792 160 Size: 872 2379 1381 1606 Fragments arranged by size: 2379 1606 1381 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 AvaI C'yCGr_G Cuts at: 1870 1943 3563 1870 Size: 73 1620 4545 Fragments arranged by size: 4545 1620 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2365 2398 2365 Size: 33 6205 BamHI G'GATC_C Cuts at: 3568 3935 3568 Size: 367 5871 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2865 3559 5014 618 Size: 325 217 627 1078 694 1455 1842 Fragments arranged by size: 1842 1455 1078 694 627 325 217 BanII G_rGCy'C Cuts at: 1391 2016 2845 3557 1391 Size: 625 829 712 4072 Fragments arranged by size: 4072 829 712 625 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2738 928 Size: 971 839 4428 Fragments arranged by size: 4428 971 839 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4429 4463 1903 Size: 34 2492 34 3678 Fragments arranged by size: 3678 2492 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4130 5340 4130 Size: 1210 5028 BclI T'GATC_A Cuts at: 1548 1548 Size: 6238 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4849 136 Size: 107 122 71 631 605 3177 1525 Fragments arranged by size: 3177 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6233 6233 Size: 6238 BlpI GC'TnA_GC Cuts at: 3655 3655 Size: 6238 Bme1580I G_kGCm'C Cuts at: 1790 4299 5455 1790 Size: 2509 1156 2573 Fragments arranged by size: 2573 2509 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6238 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3969 4927 442 Size: 416 53 83 399 24 2552 958 1753 Fragments arranged by size: 2552 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6238 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2598 3339 4898 1239 Size: 1359 741 1559 2579 Fragments arranged by size: 2579 1559 1359 741 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4363 5228 5526 4363 Size: 865 298 5075 Fragments arranged by size: 5075 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4901 882 Size: 617 3402 2219 Fragments arranged by size: 3402 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4029 493 Size: 711 2825 2702 Fragments arranged by size: 2825 2702 711 BsaBI GATnn'nnATC Cuts at: 6232 6232 Size: 6238 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3453 4045 4427 5062 Size: 53 83 186 936 1920 592 382 635 Cuts at: 5062 275 Size: 1451 Fragments arranged by size: 1920 1451 936 635 592 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4671 5069 5343 5490 5901 4671 Size: 398 274 147 411 5008 Fragments arranged by size: 5008 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3449 3479 1178 Size: 30 165 30 2046 30 3937 Fragments arranged by size: 3937 2046 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3006 1186 Size: 1820 4418 BseYI C'CCAG_C Cuts at: 3302 5441 5937 3302 Size: 2139 496 3603 Fragments arranged by size: 3603 2139 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5665 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 3688 4449 5477 1540 Size: 275 186 45 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 3243 4299 4384 5455 3243 Size: 1056 85 1071 4026 Fragments arranged by size: 4026 1071 1056 85 BsmI GAATG_Cn' Cuts at: 3749 3842 3749 Size: 93 6145 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2845 3243 3557 4299 4384 5455 Size: 399 226 829 398 314 742 85 1071 Cuts at: 5455 1391 Size: 2174 Fragments arranged by size: 2174 1071 829 742 399 398 314 226 85 BspEI T'CCGG_A Cuts at: 5901 5901 Size: 6238 BspHI T'CATG_A Cuts at: 2455 4127 5857 2455 Size: 1672 1730 2836 Fragments arranged by size: 2836 1730 1672 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2677 3593 844 Size: 1833 916 3489 Fragments arranged by size: 3489 1833 916 BsrBI CCG'CTC Cuts at: 1394 1823 4125 1394 Size: 429 2302 3507 Fragments arranged by size: 3507 2302 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3517 4733 4907 65 Size: 1183 2269 1216 174 1396 Fragments arranged by size: 2269 1396 1216 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 4882 5069 1700 Size: 110 275 2797 187 2869 Fragments arranged by size: 2869 2797 275 187 110 BsrGI T'GTAC_A Cuts at: 96 2874 96 Size: 2778 3460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6238 BssSI C'ACGA_G Cuts at: 2127 4298 5310 2127 Size: 2171 1012 3055 Fragments arranged by size: 3055 2171 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3170 3334 794 Size: 2376 164 3698 Fragments arranged by size: 3698 2376 164 BstBI TT'CG_AA Cuts at: 5042 5042 Size: 6238 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6238 BstYI r'GATC_y Cuts at: 1972 2321 3201 3568 3935 4320 4337 5789 5875 Size: 349 880 367 367 385 17 1452 86 Cuts at: 5875 5887 6233 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 880 385 367 367 349 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2947 2947 Size: 6238 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5686 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5499 Fragments arranged by size: 5499 683 56 BtsI GCAGTG_nn' Cuts at: 3754 4548 4568 3754 Size: 794 20 5424 Fragments arranged by size: 5424 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6238 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6203 DraI TTT'AAA Cuts at: 2491 3542 2491 Size: 1051 5187 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5245 809 Size: 507 3929 1802 Fragments arranged by size: 3929 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3222 3685 4574 Size: 54 1425 50 275 18 1392 463 889 Cuts at: 4574 6203 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1392 889 463 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3685 1537 Size: 275 1873 4090 Fragments arranged by size: 4090 1873 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2169 2351 3243 4168 1353 Size: 816 182 892 925 3423 Fragments arranged by size: 3423 925 892 816 182 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4825 5199 5345 387 Size: 1064 138 3236 374 146 1280 Fragments arranged by size: 3236 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2362 2598 3339 4301 4898 5685 5953 Size: 393 730 236 741 962 597 787 268 Cuts at: 5953 1239 Size: 1524 Fragments arranged by size: 1524 962 787 741 730 597 393 268 236 EcoRI G'AATT_C Cuts at: 2804 3547 3925 2804 Size: 743 378 5117 Fragments arranged by size: 5117 743 378 EcoRV GAT'ATC Cuts at: 2137 2638 2692 2137 Size: 501 54 5683 Fragments arranged by size: 5683 501 54 FspI TGC'GCA Cuts at: 4744 4744 Size: 6238 HaeII r_GCGC'y Cuts at: 1678 3345 5385 1678 Size: 1667 2040 2531 Fragments arranged by size: 2531 2040 1667 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 3412 3444 4879 Size: 32 135 32 623 32 1185 32 1435 Cuts at: 4879 4911 4953 4985 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1435 1185 623 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2040 2365 3582 3828 669 Size: 988 281 102 325 1217 246 3079 Fragments arranged by size: 3079 1217 988 325 281 246 102 HindIII A'AGCT_T Cuts at: 748 748 Size: 6238 HpaI GTT'AAC Cuts at: 3828 3828 Size: 6238 KpnI G_GTAC'C Cuts at: 2869 3563 2869 Size: 694 5544 MfeI C'AATT_G Cuts at: 2022 3837 2022 Size: 1815 4423 MluI A'CGCG_T Cuts at: 2521 6114 2521 Size: 3593 2645 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5352 5536 1305 Size: 4047 184 2007 Fragments arranged by size: 4047 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3224 10 Size: 54 1425 343 1392 3024 Fragments arranged by size: 3024 1425 1392 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4196 4555 4714 518 Size: 648 3030 359 159 2042 Fragments arranged by size: 3030 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3170 3306 3654 4331 5479 5724 1606 Size: 1564 136 348 677 1148 245 2120 Fragments arranged by size: 2120 1564 1148 677 348 245 136 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5686 NdeI CA'TA_TG Cuts at: 387 2674 387 Size: 2287 3951 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6238 NotI GC'GGCC_GC Cuts at: 3685 3685 Size: 6238 NspI r_CATG'y Cuts at: 1132 1229 2875 3298 3596 5141 1132 Size: 97 1646 423 298 1545 2229 Fragments arranged by size: 2229 1646 1545 423 298 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6238 PciI A'CATG_T Cuts at: 5137 5137 Size: 6238 PflMI CCAn_nnn'nTGG Cuts at: 1108 3074 1108 Size: 1966 4272 PmeI GTTT'AAAC Cuts at: 3542 3542 Size: 6238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4307 4339 5820 5852 4307 Size: 32 1481 32 4693 Fragments arranged by size: 4693 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6238 PsiI TTA'TAA Cuts at: 3808 3808 Size: 6238 PstI C_TGCA'G Cuts at: 830 2965 3175 3590 830 Size: 2135 210 415 3478 Fragments arranged by size: 3478 2135 415 210 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6238 PvuII CAG'CTG Cuts at: 3170 3170 Size: 6238 SalI G'TCGA_C Cuts at: 1936 2038 3580 1936 Size: 102 1542 4594 Fragments arranged by size: 4594 1542 102 SbfI CC_TGCA'GG Cuts at: 3590 3590 Size: 6238 ScaI AGT'ACT Cuts at: 3312 4486 6198 3312 Size: 1174 1712 3352 Fragments arranged by size: 3352 1712 1174 SfcI C'TryA_G Cuts at: 826 1045 2782 2961 3171 3586 4721 5402 5593 Size: 219 1737 179 210 415 1135 681 191 Cuts at: 5593 826 Size: 1471 Fragments arranged by size: 1737 1471 1135 681 415 219 210 191 179 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6238 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6238 SmaI CCC'GGG Cuts at: 1872 3565 1872 Size: 1693 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4342 5243 5505 820 Size: 197 926 2399 901 262 1553 Fragments arranged by size: 2399 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4029 493 Size: 3536 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6238 SphI G_CATG'C Cuts at: 1132 3298 3596 1132 Size: 2166 298 3774 Fragments arranged by size: 3774 2166 298 SspI AAT'ATT Cuts at: 5 52 3532 4162 5 Size: 47 3480 630 2081 Fragments arranged by size: 3480 2081 630 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6238 StyI C'CwwG_G Cuts at: 513 1065 2016 3393 3677 513 Size: 552 951 1377 284 3074 Fragments arranged by size: 3074 1377 951 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2667 3420 4265 4282 4435 4620 Size: 1255 480 607 753 845 17 153 185 Cuts at: 4620 5065 325 Size: 445 1498 Fragments arranged by size: 1498 1255 845 753 607 480 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2874 3310 4484 Size: 274 80 33 51 1443 897 436 1174 Cuts at: 4484 6196 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1174 897 436 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6198 6230 6198 Size: 32 6206 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6238 XbaI T'CTAG_A Cuts at: 3574 3574 Size: 6238 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6238 XmaI C'CCGG_G Cuts at: 1870 3563 1870 Size: 1693 4545 XmnI GAAnn'nnTTC Cuts at: 4367 4367 Size: 6238 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4046 276 Size: 53 83 186 936 2512 2468 Fragments arranged by size: 2512 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI