(Circular) (Six-base) MAPSORT of: pFN21AB9500.seq Check: 7174 from: 1 to: 6730 pFN21AB9500 6730 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4540 278 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4073 1937 Size: 2136 4594 Acc65I G'GTAC_C Cuts at: 4009 4051 4009 Size: 42 6688 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3879 4793 6177 6445 1632 Size: 2247 914 1384 268 1917 Fragments arranged by size: 2247 1917 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 2264 820 Size: 197 1247 5286 Fragments arranged by size: 5286 1247 197 AflIII A'CryG_T Cuts at: 1203 5629 6606 1203 Size: 4426 977 1327 Fragments arranged by size: 4426 1327 977 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 2965 5459 2965 Size: 2494 4236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6730 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6730 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2889 2921 2889 Size: 32 6698 AlwNI CAG_nnn'CTG Cuts at: 4473 6045 4473 Size: 1572 5158 ApaLI G'TGCA_C Cuts at: 2163 4787 5943 2163 Size: 2624 1156 2950 Fragments arranged by size: 2950 2624 1156 ApoI r'AATT_y Cuts at: 1493 2280 2928 3653 3664 3763 3937 4039 4265 Size: 787 648 725 11 99 174 102 226 Cuts at: 4265 4417 6526 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 787 725 648 226 174 152 102 99 11 AseI AT'TA_AT Cuts at: 160 1032 2916 5284 160 Size: 872 1884 2368 1606 Fragments arranged by size: 2368 1884 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 4055 1870 Size: 73 2112 4545 Fragments arranged by size: 4545 2112 73 BamHI G'GATC_C Cuts at: 4060 4427 4060 Size: 367 6363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2135 4009 4051 5506 618 Size: 325 217 627 348 1874 42 1455 1842 Fragments arranged by size: 1874 1842 1455 627 348 325 217 42 BanII G_rGCy'C Cuts at: 1391 2712 4049 1391 Size: 1321 1337 4072 Fragments arranged by size: 4072 1337 1321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2385 3725 3758 3890 928 Size: 971 486 1340 33 132 3768 Fragments arranged by size: 3768 1340 971 486 132 33 BbvCI CC'TCA_GC Cuts at: 4025 4025 Size: 6730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2666 2700 4921 4955 1903 Size: 34 729 34 2221 34 3678 Fragments arranged by size: 3678 2221 729 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4622 5832 4622 Size: 1210 5520 BclI T'GATC_A Cuts at: 1548 3147 3702 1548 Size: 1599 555 4576 Fragments arranged by size: 4576 1599 555 BfrBI ATG'CAT Cuts at: 2489 2977 2489 Size: 488 6242 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5341 136 Size: 107 122 71 631 605 3669 1525 Fragments arranged by size: 3669 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2566 3390 6725 2566 Size: 824 3335 2571 Fragments arranged by size: 3335 2571 824 BlpI GC'TnA_GC Cuts at: 2688 4147 2688 Size: 1459 5271 Bme1580I G_kGCm'C Cuts at: 1790 2138 2167 4791 5947 1790 Size: 348 29 2624 1156 2573 Fragments arranged by size: 2624 2573 1156 348 29 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6730 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3000 4461 5419 Size: 416 53 83 399 24 1583 1461 958 Cuts at: 5419 442 Size: 1753 Fragments arranged by size: 1753 1583 1461 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2145 5390 1239 Size: 906 3245 2579 Fragments arranged by size: 3245 2579 906 Bpu10I CC'TnA_GC Cuts at: 3971 4025 3971 Size: 54 6676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2559 4855 5720 6018 2559 Size: 2296 865 298 3271 Fragments arranged by size: 3271 2296 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3436 3555 5393 882 Size: 617 1937 119 1838 2219 Fragments arranged by size: 2219 1937 1838 617 119 BsaAI yAC'GTr Cuts at: 493 1204 4521 493 Size: 711 3317 2702 Fragments arranged by size: 3317 2702 711 BsaBI GATnn'nnATC Cuts at: 6724 6724 Size: 6730 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4537 4919 5554 275 Size: 53 83 186 936 3004 382 635 1451 Fragments arranged by size: 3004 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2294 3759 5163 5561 5835 5982 6393 2294 Size: 1465 1404 398 274 147 411 2631 Fragments arranged by size: 2631 1465 1404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2748 2778 2889 2919 1178 Size: 30 165 30 1345 30 111 30 4989 Fragments arranged by size: 4989 1345 165 111 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2769 3401 1186 Size: 1583 632 4515 Fragments arranged by size: 4515 1583 632 BseYI C'CCAG_C Cuts at: 5933 6429 5933 Size: 496 6234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4180 4941 5969 1540 Size: 275 186 2179 761 1028 2301 Fragments arranged by size: 2301 2179 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2167 4791 4876 5947 2167 Size: 2624 85 1071 2950 Fragments arranged by size: 2950 2624 1071 85 BsmI GAATG_Cn' Cuts at: 2491 3608 4241 4334 2491 Size: 1117 633 93 4887 Fragments arranged by size: 4887 1117 633 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2167 2712 4049 4791 4876 5947 Size: 399 348 29 545 1337 742 85 1071 Cuts at: 5947 1391 Size: 2174 Fragments arranged by size: 2174 1337 1071 742 545 399 348 85 29 BspEI T'CCGG_A Cuts at: 3759 6393 3759 Size: 2634 4096 BspHI T'CATG_A Cuts at: 3501 4619 6349 3501 Size: 1118 1730 3882 Fragments arranged by size: 3882 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4085 844 Size: 3241 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4617 1394 Size: 429 2794 3507 Fragments arranged by size: 3507 2794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3792 5225 5399 65 Size: 1183 2544 1433 174 1396 Fragments arranged by size: 2544 1433 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3575 5374 5561 1700 Size: 110 1765 1799 187 2869 Fragments arranged by size: 2869 1799 1765 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6730 BssSI C'ACGA_G Cuts at: 2790 4790 5802 2790 Size: 2000 1012 3718 Fragments arranged by size: 3718 2000 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2698 794 Size: 1904 4826 BstBI TT'CG_AA Cuts at: 5534 5534 Size: 6730 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6730 BstXI CCAn_nnnn'nTGG Cuts at: 2024 3171 4020 2024 Size: 1147 849 4734 Fragments arranged by size: 4734 1147 849 BstYI r'GATC_y Cuts at: 1972 2566 3160 3390 4060 4427 4812 4829 6281 Size: 594 594 230 670 367 385 17 1452 Cuts at: 6281 6367 6379 6725 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 670 594 594 385 367 346 230 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6178 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5991 Fragments arranged by size: 5991 683 56 BtsI GCAGTG_nn' Cuts at: 2447 4246 5040 5060 2447 Size: 1799 794 20 4117 Fragments arranged by size: 4117 1799 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6730 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6695 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5737 809 Size: 507 4421 1802 Fragments arranged by size: 4421 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2743 3475 3831 Size: 54 1425 50 275 18 913 732 356 Cuts at: 3831 3910 4177 5066 6695 8 Size: 79 267 889 1629 43 Fragments arranged by size: 1629 1425 913 889 732 356 275 267 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4177 1537 Size: 275 2365 4090 Fragments arranged by size: 4090 2365 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3329 3434 3855 4660 1353 Size: 1976 105 421 805 3423 Fragments arranged by size: 3423 1976 805 421 105 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5317 5691 5837 387 Size: 1064 138 3728 374 146 1280 Fragments arranged by size: 3728 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2145 3879 4793 5390 6177 6445 1239 Size: 393 513 1734 914 597 787 268 1524 Fragments arranged by size: 1734 1524 914 787 597 513 393 268 EcoRI G'AATT_C Cuts at: 3763 3937 4039 4417 3763 Size: 174 102 378 6076 Fragments arranged by size: 6076 378 174 102 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3538 3833 3538 Size: 295 6435 FspI TGC'GCA Cuts at: 5236 5236 Size: 6730 HaeII r_GCGC'y Cuts at: 1678 5877 1678 Size: 4199 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2951 2983 2992 Size: 32 135 32 488 32 859 32 9 Cuts at: 2992 3024 3395 3427 3435 3467 5371 5403 5445 Size: 32 371 32 8 32 1904 32 42 Cuts at: 5445 5477 1373 Size: 32 2626 Fragments arranged by size: 2626 1904 859 488 371 135 42 32 32 32 32 32 32 32 32 32 9 8 HincII GTy'rAC Cuts at: 669 1657 1938 2116 3011 4074 4320 669 Size: 988 281 178 895 1063 246 3079 Fragments arranged by size: 3079 1063 988 895 281 246 178 HindIII A'AGCT_T Cuts at: 748 3235 748 Size: 2487 4243 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KpnI G_GTAC'C Cuts at: 4013 4055 4013 Size: 42 6688 MfeI C'AATT_G Cuts at: 3930 4329 3930 Size: 399 6331 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2151 2431 2580 5844 6028 1305 Size: 846 280 149 3264 184 2007 Fragments arranged by size: 3264 2007 846 280 184 149 MscI TGG'CCA Cuts at: 10 64 1489 1832 2745 3477 3833 3912 10 Size: 54 1425 343 913 732 356 79 2828 Fragments arranged by size: 2828 1425 913 732 356 343 79 54 MslI CAynn'nnrTG Cuts at: 518 1166 2022 2141 4688 5047 5206 518 Size: 648 856 119 2547 359 159 2042 Fragments arranged by size: 2547 2042 856 648 359 159 119 MspA1I CmG'CkG Cuts at: 1606 3629 4146 4823 5971 6216 1606 Size: 2023 517 677 1148 245 2120 Fragments arranged by size: 2120 2023 1148 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6178 NdeI CA'TA_TG Cuts at: 387 2019 387 Size: 1632 5098 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 4177 4177 Size: 6730 NsiI A_TGCA'T Cuts at: 2491 2979 2491 Size: 488 6242 NspI r_CATG'y Cuts at: 1132 1229 2033 4088 5633 1132 Size: 97 804 2055 1545 2229 Fragments arranged by size: 2229 2055 1545 804 97 PacI TTA_AT'TAA Cuts at: 3981 3981 Size: 6730 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6730 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6730 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2889 2921 4799 4831 6312 6344 2889 Size: 32 1878 32 1481 32 3275 Fragments arranged by size: 3275 1878 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2993 1613 Size: 1380 5350 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2757 2789 2757 Size: 32 6698 PstI C_TGCA'G Cuts at: 830 2872 3535 4082 830 Size: 2042 663 547 3478 Fragments arranged by size: 3478 2042 663 547 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 3629 3629 Size: 6730 SalI G'TCGA_C Cuts at: 1936 4072 1936 Size: 2136 4594 SbfI CC_TGCA'GG Cuts at: 4082 4082 Size: 6730 ScaI AGT'ACT Cuts at: 4978 6690 4978 Size: 1712 5018 SfcI C'TryA_G Cuts at: 826 1045 2811 2868 3181 3531 4078 5213 5894 Size: 219 1766 57 313 350 547 1135 681 Cuts at: 5894 6085 826 Size: 191 1471 Fragments arranged by size: 1766 1471 1135 681 547 350 313 219 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 4057 1872 Size: 2185 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 2574 4834 5735 5997 820 Size: 197 926 321 310 2260 901 262 1553 Fragments arranged by size: 2260 1553 926 901 321 310 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 4088 1132 Size: 2956 3774 SspI AAT'ATT Cuts at: 5 52 2367 4654 5 Size: 47 2315 2287 2081 Fragments arranged by size: 2315 2287 2081 47 StuI AGG'CCT Cuts at: 1517 3179 1517 Size: 1662 5068 StyI C'CwwG_G Cuts at: 513 1065 3033 4169 513 Size: 552 1968 1136 3074 Fragments arranged by size: 3074 1968 1136 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 4757 4774 4927 5112 5557 325 Size: 1255 1215 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 1215 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2515 4976 6688 Size: 274 80 33 51 1443 538 2461 1712 Cuts at: 6688 96 Size: 138 Fragments arranged by size: 2461 1712 1443 538 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6690 6722 6690 Size: 32 6698 Tth111I GACn'n_nGTC Cuts at: 1316 2834 1316 Size: 1518 5212 XbaI T'CTAG_A Cuts at: 2908 4066 2908 Size: 1158 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6730 XmaI C'CCGG_G Cuts at: 1870 4055 1870 Size: 2185 4545 XmnI GAAnn'nnTTC Cuts at: 3816 4859 3816 Size: 1043 5687 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4538 276 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI