(Circular) (Six-base) MAPSORT of: pFN21AB9498.seq Check: 4954 from: 1 to: 6538 pFN21AB9498 6538 bp With 182 enzymes: SgfI * February 27, 2009 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4348 278 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3881 1937 Size: 1944 4594 Acc65I G'GTAC_C Cuts at: 3859 3859 Size: 6538 AclI AA'CG_TT Cuts at: 1563 4665 5038 1563 Size: 3102 373 3063 Fragments arranged by size: 3102 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4601 5985 6253 1632 Size: 2969 1384 268 1917 Fragments arranged by size: 2969 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6341 AflIII A'CryG_T Cuts at: 1203 5437 6414 1203 Size: 4234 977 1327 Fragments arranged by size: 4234 1327 977 AgeI A'CCGG_T Cuts at: 2697 5369 2697 Size: 2672 3866 AhdI GACnn_n'nnGTC Cuts at: 5267 5267 Size: 6538 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6538 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6538 AlwNI CAG_nnn'CTG Cuts at: 4281 5853 4281 Size: 1572 4966 ApaLI G'TGCA_C Cuts at: 4595 5751 4595 Size: 1156 5382 ApoI r'AATT_y Cuts at: 1493 2345 2432 2499 3847 4073 4225 6334 1493 Size: 852 87 67 1348 226 152 2109 1697 Fragments arranged by size: 2109 1697 1348 852 226 152 87 67 AseI AT'TA_AT Cuts at: 160 1032 5092 160 Size: 872 4060 1606 Fragments arranged by size: 4060 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 AvaI C'yCGr_G Cuts at: 1870 1943 2244 2955 3069 3204 3863 1870 Size: 73 301 711 114 135 659 4545 Fragments arranged by size: 4545 711 659 301 135 114 73 BamHI G'GATC_C Cuts at: 3868 4235 3868 Size: 367 6171 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2197 3859 5314 618 Size: 325 217 627 410 1662 1455 1842 Fragments arranged by size: 1842 1662 1455 627 410 325 217 BanII G_rGCy'C Cuts at: 1391 2608 2939 3497 3857 1391 Size: 1217 331 558 360 4072 Fragments arranged by size: 4072 1217 558 360 331 BbeI G_GCGC'C Cuts at: 2201 2201 Size: 6538 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2048 2264 2907 928 Size: 971 149 216 643 4559 Fragments arranged by size: 4559 971 643 216 149 BbvCI CC'TCA_GC Cuts at: 2014 2070 2014 Size: 56 6482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4729 4763 1903 Size: 34 2792 34 3678 Fragments arranged by size: 3678 2792 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4430 5640 4430 Size: 1210 5328 BclI T'GATC_A Cuts at: 1548 2518 3406 1548 Size: 970 888 4680 Fragments arranged by size: 4680 970 888 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5149 136 Size: 107 122 71 631 605 337 3140 1525 Fragments arranged by size: 3140 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 3716 6533 3716 Size: 2817 3721 BlpI GC'TnA_GC Cuts at: 3955 3955 Size: 6538 Bme1580I G_kGCm'C Cuts at: 1790 2464 4599 5755 1790 Size: 674 2135 1156 2573 Fragments arranged by size: 2573 2135 1156 674 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6538 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3055 3294 3633 Size: 416 53 83 399 24 1638 239 339 Cuts at: 3633 4269 5227 442 Size: 636 958 1753 Fragments arranged by size: 1753 1638 958 636 416 399 339 239 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6538 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2620 2923 3528 3697 5198 1239 Size: 1381 303 605 169 1501 2579 Fragments arranged by size: 2579 1501 1381 605 303 169 Bpu10I CC'TnA_GC Cuts at: 2014 2070 3726 2014 Size: 56 1656 4826 Fragments arranged by size: 4826 1656 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4663 5528 5826 4663 Size: 865 298 5375 Fragments arranged by size: 5375 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3657 5201 882 Size: 617 2158 1544 2219 Fragments arranged by size: 2219 2158 1544 617 BsaAI yAC'GTr Cuts at: 493 1204 2387 4329 493 Size: 711 1183 1942 2702 Fragments arranged by size: 2702 1942 1183 711 BsaBI GATnn'nnATC Cuts at: 2568 2712 2724 2751 6532 2568 Size: 144 12 27 3781 2574 Fragments arranged by size: 3781 2574 144 27 12 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2198 4345 4727 5362 Size: 53 83 186 936 665 2147 382 635 Cuts at: 5362 275 Size: 1451 Fragments arranged by size: 2147 1451 936 665 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2262 2697 4971 5369 5643 5790 6201 2262 Size: 435 2274 398 274 147 411 2599 Fragments arranged by size: 2599 2274 435 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2593 2623 3313 3343 1178 Size: 30 165 30 1190 30 690 30 4373 Fragments arranged by size: 4373 1190 690 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2895 1186 Size: 1709 4829 BseYI C'CCAG_C Cuts at: 2121 2393 5741 6237 2121 Size: 272 3348 496 2422 Fragments arranged by size: 3348 2422 496 272 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2391 3705 1163 Size: 573 655 1314 3996 Fragments arranged by size: 3996 1314 655 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2263 2930 3257 3988 4749 5777 Size: 275 186 262 667 327 731 761 1028 Cuts at: 5777 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 731 667 327 275 262 186 BsiHKAI G_wGCw'C Cuts at: 2939 3497 4599 4684 5755 2939 Size: 558 1102 85 1071 3722 Fragments arranged by size: 3722 1102 1071 558 85 BsmI GAATG_Cn' Cuts at: 3454 3610 4049 4142 3454 Size: 156 439 93 5850 Fragments arranged by size: 5850 439 156 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3642 3642 Size: 6538 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2464 2608 2939 3497 3857 4599 4684 Size: 399 674 144 331 558 360 742 85 Cuts at: 4684 5755 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 674 558 399 360 331 144 85 BspEI T'CCGG_A Cuts at: 6201 6201 Size: 6538 BspHI T'CATG_A Cuts at: 4427 6157 4427 Size: 1730 4808 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3893 844 Size: 3049 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4425 1394 Size: 429 2602 3507 Fragments arranged by size: 3507 2602 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5033 5207 65 Size: 1183 3785 174 1396 Fragments arranged by size: 3785 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2697 5182 5369 1700 Size: 110 887 2485 187 2869 Fragments arranged by size: 2869 2485 887 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6538 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6538 BssSI C'ACGA_G Cuts at: 4598 5610 4598 Size: 1012 5526 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6538 BstBI TT'CG_AA Cuts at: 5342 5342 Size: 6538 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6538 BstXI CCAn_nnnn'nTGG Cuts at: 2121 2121 Size: 6538 BstYI r'GATC_y Cuts at: 1972 3716 3868 4235 4620 4637 6089 6175 6187 Size: 1744 152 367 385 17 1452 86 12 Cuts at: 6187 6533 1972 Size: 346 1977 Fragments arranged by size: 1977 1744 1452 385 367 346 152 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3030 3741 3030 Size: 711 5827 BtgI C'CryG_G Cuts at: 513 1065 2004 2234 3637 513 Size: 552 939 230 1403 3414 Fragments arranged by size: 3414 1403 939 552 230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5799 Fragments arranged by size: 5799 683 56 BtsI GCAGTG_nn' Cuts at: 2226 2292 2379 2836 2857 4054 4848 4868 2226 Size: 66 87 457 21 1197 794 20 3896 Fragments arranged by size: 3896 1197 794 457 87 66 21 20 ClaI AT'CG_AT Cuts at: 1554 2755 1554 Size: 1201 5337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6503 DraI TTT'AAA Cuts at: 3842 3842 Size: 6538 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5545 809 Size: 507 4229 1802 Fragments arranged by size: 4229 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2557 2764 3985 Size: 54 1425 50 275 18 727 207 1221 Cuts at: 3985 4874 6503 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1221 889 727 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3985 1537 Size: 275 2173 4090 Fragments arranged by size: 4090 2173 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2472 2641 2833 4468 1353 Size: 972 147 169 192 1635 3423 Fragments arranged by size: 3423 1635 972 192 169 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5125 5499 5645 387 Size: 1064 138 3536 374 146 1280 Fragments arranged by size: 3536 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2937 3495 2937 Size: 558 5980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2620 2923 3528 3697 4601 5198 5985 Size: 393 988 303 605 169 904 597 787 Cuts at: 5985 6253 1239 Size: 268 1524 Fragments arranged by size: 1524 988 904 787 605 597 393 303 268 169 EcoNI CCTnn'n_nnAGG Cuts at: 2646 2646 Size: 6538 EcoO109I rG'GnC_Cy Cuts at: 2440 2984 3288 2440 Size: 544 304 5690 Fragments arranged by size: 5690 544 304 EcoRI G'AATT_C Cuts at: 2345 2499 3847 4225 2345 Size: 154 1348 378 4658 Fragments arranged by size: 4658 1348 378 154 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2365 2365 Size: 6538 FspI TGC'GCA Cuts at: 5044 5044 Size: 6538 HaeII r_GCGC'y Cuts at: 1678 2201 5685 1678 Size: 523 3484 2531 Fragments arranged by size: 3484 2531 523 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2349 2381 2593 2625 2912 Size: 32 135 32 777 32 212 32 287 Cuts at: 2912 2936 2944 2968 3029 3061 3362 3394 3497 Size: 24 8 24 61 32 301 32 103 Cuts at: 3497 3529 3695 3701 3727 3733 5179 5211 5253 Size: 32 166 6 26 6 1446 32 42 Cuts at: 5253 5285 1373 Size: 32 2626 Fragments arranged by size: 2626 1446 777 301 287 212 166 135 103 61 42 32 32 32 32 32 32 32 32 32 26 24 24 8 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3106 3882 4128 669 Size: 988 281 1168 776 246 3079 Fragments arranged by size: 3079 1168 988 776 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6538 HpaI GTT'AAC Cuts at: 4128 4128 Size: 6538 KasI G'GCGC_C Cuts at: 2197 2197 Size: 6538 KpnI G_GTAC'C Cuts at: 3863 3863 Size: 6538 MfeI C'AATT_G Cuts at: 2205 4137 2205 Size: 1932 4606 MluI A'CGCG_T Cuts at: 6414 6414 Size: 6538 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3407 5652 5836 1305 Size: 2102 2245 184 2007 Fragments arranged by size: 2245 2102 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4716 Fragments arranged by size: 4716 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4496 4855 5014 518 Size: 648 3330 359 159 2042 Fragments arranged by size: 3330 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2121 3113 3175 3954 4631 5779 6024 1606 Size: 515 992 62 779 677 1148 245 2120 Fragments arranged by size: 2120 1148 992 779 677 515 245 62 NarI GG'CG_CC Cuts at: 2198 2198 Size: 6538 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5047 Fragments arranged by size: 5047 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6538 NotI GC'GGCC_GC Cuts at: 3985 3985 Size: 6538 NspI r_CATG'y Cuts at: 1132 1229 3896 5441 1132 Size: 97 2667 1545 2229 Fragments arranged by size: 2667 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6538 PciI A'CATG_T Cuts at: 5437 5437 Size: 6538 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6538 PfoI T'CCnGG_A Cuts at: 2819 3643 2819 Size: 824 5714 PmeI GTTT'AAAC Cuts at: 3842 3842 Size: 6538 PmlI CAC'GTG Cuts at: 2387 2387 Size: 6538 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4607 4639 6120 6152 4607 Size: 32 1481 32 4993 Fragments arranged by size: 4993 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2440 2984 3288 2440 Size: 544 304 5690 Fragments arranged by size: 5690 544 304 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6538 PsiI TTA'TAA Cuts at: 4108 4108 Size: 6538 PspXI vC'TCGA_Gb Cuts at: 3069 3069 Size: 6538 PstI C_TGCA'G Cuts at: 830 3890 830 Size: 3060 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6538 PvuII CAG'CTG Cuts at: 2121 3113 3175 2121 Size: 992 62 5484 Fragments arranged by size: 5484 992 62 SacI G_AGCT'C Cuts at: 2939 3497 2939 Size: 558 5980 SalI G'TCGA_C Cuts at: 1936 3880 1936 Size: 1944 4594 SapI GCTCTTCn'nnn_ Cuts at: 2833 2833 Size: 6538 SbfI CC_TGCA'GG Cuts at: 3890 3890 Size: 6538 ScaI AGT'ACT Cuts at: 3025 4786 6498 3025 Size: 1761 1712 3065 Fragments arranged by size: 3065 1761 1712 SfcI C'TryA_G Cuts at: 826 1045 2616 2875 3143 3886 5021 5702 5893 Size: 219 1571 259 268 743 1135 681 191 Cuts at: 5893 826 Size: 1471 Fragments arranged by size: 1571 1471 1135 743 681 268 259 219 191 SfoI GGC'GCC Cuts at: 2199 2199 Size: 6538 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6538 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6538 SmaI CCC'GGG Cuts at: 1872 3865 1872 Size: 1993 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3069 4642 5543 5805 820 Size: 197 926 1126 1573 901 262 1553 Fragments arranged by size: 1573 1553 1126 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4329 493 Size: 3836 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6538 SphI G_CATG'C Cuts at: 1132 3896 1132 Size: 2764 3774 SspI AAT'ATT Cuts at: 5 52 4462 5 Size: 47 4410 2081 Fragments arranged by size: 4410 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6538 StyI C'CwwG_G Cuts at: 513 1065 2004 3977 513 Size: 552 939 1973 3074 Fragments arranged by size: 3074 1973 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2799 2916 2919 3231 3445 4565 4582 Size: 1255 1219 117 3 312 214 1120 17 Cuts at: 4582 4735 4920 5365 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1219 1120 445 312 214 185 153 117 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3023 4784 6496 Size: 274 80 33 51 1443 1046 1761 1712 Cuts at: 6496 96 Size: 138 Fragments arranged by size: 1761 1712 1443 1046 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2815 2847 3253 3285 3313 3345 6498 6530 2815 Size: 32 406 32 28 32 3153 32 2823 Fragments arranged by size: 3153 2823 406 32 32 32 32 28 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6538 XbaI T'CTAG_A Cuts at: 3340 3874 3340 Size: 534 6004 XhoI C'TCGA_G Cuts at: 1943 3069 1943 Size: 1126 5412 XmaI C'CCGG_G Cuts at: 1870 3863 1870 Size: 1993 4545 XmnI GAAnn'nnTTC Cuts at: 2349 4667 2349 Size: 2318 4220 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4346 276 Size: 53 83 186 936 2812 2468 Fragments arranged by size: 2812 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI